目次 [Contents]
- 遺伝子材料開発室(DNABank)は、文部科学省・日本学術振興会による科研費「学術変革領域研究(A) 2023-2027年度 細胞外情報を統御するマルチモーダルECM」にコアメンバーとして参画しており、その研究過程で構築・整備されたコラーゲンcDNAの提供を開始いたしました。
- 全コラーゲンファミリー ヒト: 28タイプ 44遺伝子、マウス: 27タイプ 44遺伝子のうち、約9割をカバーするヒト25タイプ 33遺伝子、マウス22タイプ 38遺伝子のコラーゲンcDNAプラスミドを取り揃えております。
- 以下のコラーゲンcDNAプラスミドが提供可能です。各Order No.のリンク先からご依頼いただけます。
- DNABank is a core member of the Grant-in-Aid for Transformative Research Areas (A) Integration of Extracellular Information by Multimodal ECM Activity. Collagen cDNA developed in the course of this research is now available.
- Collagen cDNA plasmids of 25 types and 33 genes in human and 22 types and 38 genes in mouse are available, covering approximately 90% of the total collagen family: 28 types and 44 genes in human and 27 types and 44 genes in mouse.
- The following collagen cDNA plasmids are available. You can request from the link of each Order number.
| Genetic information | Function | Phenotype | cDNA Plasmid Catalog | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Collagen type | Alpha chain | Human (a.a.) | Mouse (a.a.) | RefSeq (Human) | Class | Knockout (Mouse) ＊ | IMPC (Mouse) | Order No. (Human) | Order No. (Mouse) |
| Collagen type 1 | α1 | 1464 | 1453 | NM_000088.4 | fibril | embryonic or adolescent lethality | Col1a1 | HGY018997 | RDB20930 |
| Collagen type 1 | α2 | 1366 | 1372 | NM_000089.4 | fibril | decrease body size | Col1a2 | HGX016923 | RDB20931 |
| Collagen type 2 | α1 | 1487 | 1487 | NM_001844.5 | fibril | decrease body size | Col2a1 | RDB20795 | RDB20932 |
| Collagen type 3 | α1 | 1466 | 1464 | NM_000090.4 | fibril | premature death | Col3a1 | HGX025171 | RDB20933 |
| Collagen type 4 | α1 | 1669 | 1669 | NM_001845.6 | network-forming | embryos developed up to E9.5 | Col4a1 | HKR164952 | RDB20934 |
| Collagen type 4 | α2 | 1712 | 1707 | NM_001846.4 | network-forming | embrionic lethal | Col4a2 | HKR235541 | RDB20935 |
| Collagen type 4 | α3 | 1670 | 1669 | NM_000091.5 | network-forming | premature death | Col4a3 | - | - |
| Collagen type 4 | α4 | 1690 | 1682 | NM_000092.5 | network-forming | N.A. (not applicable) | Col4a4 | RDB20796 | RDB20997 |
| Collagen type 4 | α5 | 1685 | 1691 | NM_033380.3 | network-forming | N.A. (not applicable) | Col4a5 | RDB20797 | RDB20936 |
| Collagen type 4 | α6 | 1691 | 1691 | NM_033641.4 | network-forming | N.A. (not applicable) | Col4a6 | RDB20798 | RDB20937 |
| Collagen type 5 | α1 | 1838 | 1838 | NM_000093.5 | fibril | embrionic lethal | Col5a1 | HKR071631 | RDB20938 |
| Collagen type 5 | α2 | 1499 | 1497 | NM_000393.5 | fibril | poor survive | Col5a2 | RDB20799 | RDB20939 |
| Collagen type 5 | α3 | 1745 | 1739 | NM_015719.4 | fibril | reduced dermal fat, resistant to high-fat diet-induced weight gain, glucose intolerant, insulin-resistant, and hyperglycemic, and these metabolic defects worsened with age. | Col5a3 | RDB20800 | RDB20940 |
| Collagen type 6 | α1 | 1028 | 1025 | NM_001848.3 | beaded filaments | fiber necrosis and phagocytosis, pronounced variation in the fiber diameter, stimulated regeneration of fibers | Col6a1 | RDB20801 | RDB20941 |
| Collagen type 6 | α2 | 1019 | 1034 | NM_001849.4 | beaded filaments | preweaning lethality, incomplete penetrance, preweaning lethality, incomplete penetrance | Col6a2 | HGE096061 | RDB20942 |
| Collagen type 6 | α3 | 3177 | 3284 | NM_004369.4 | beaded filaments | prolonged QT interval, enlarged urinary bladder, enlarged lymph nodes, enlarged lymph nodes, decreased grip strength, decreased body length | Col6a3 | - | RDB20998 |
| Collagen type 6 | α4 | does not exist | 1367 | - | beaded filaments | not significant | Col6a4 | Not exist | RDB20999 |
| Collagen type 6 | α5 | 2526 | 2640 | NM_001278298.2 | beaded filaments | N.A. (not applicable) | Col6a5 | - | RDB20991 |
| Collagen type 6 | α6 | 2263 | 1182 | NM_001102608.3 | beaded filaments | N.A. (not applicable) | Col6a6 | - | - |
| Collagen type 7 | α1 | 2944 | 2944 | NM_000094.4 | anchoring fibrils | born with extensive cutaneous blistering, died during the first two weeks | Col7a1 | - | - |
| Collagen type 8 | α1 | 744 | 744 | NM_020351.4 | network-forming | not significant type VIII collagen is required for normal anterior eye development, particularly the formation of a corneal stroma with the appropriate number of fibroblastic cell layers | Col8a1 | HGX005608 | RDB20992 |
| Collagen type 8 | α2 | 703 | 699 | NM_005202.4 | network-forming | N.A. (not applicable) | Col8a2 | RDB20802 | RDB20945 |
| Collagen type 9 | α1 | 921 | 921 | NM_001851.6 | FACITS | no detectable abnormalities at birth but develop a severe degenerative joint disease resembling human osteoarthritis | Col9a1 | - | RDB21000 |
| Collagen type 9 | α2 | 689 | 688 | NM_001852.4 | FACITS | abnormal femur, ear, pelvic girdle bone, ulna, humerus tibia, radius morphology | Col9a2 | RDB20803 | RDB20946 |
| Collagen type 9 | α3 | 684 | 680 | NM_001853.4 | FACITS | abnormal vesicle morphology. Enlarged nodes, spleen. Decrease body length | Col9a3 | HGY090971 | RDB21001 |
| Collagen type 10 | α1 | 680 | 680 | NM_000493.4 | network-forming | No differences were detected between the type X collagen-null mice. mice and humans with dominant acting type X collagen mutations have bone abnormalities. | Col10a1 | RDB20804 | RDB20947 |
| Collagen type 11 | α1 | 1806 | 1804 | NM_001854.4 | fibril | abnormal tail, facial, limb morphology. protruding tongue, preweaning lethality, complete penetrance. increased heart left ventricle size, cleft palate | Col11a1 | - | RDB20948 |
| Collagen type 11 | α2 | 1736 | 1650 | NM_080680.3 | fibril | preweaning lethality | Col11a2 | - | RDB20949 |
| Collagen type 12 | α1 | 3063 | 3065 | NM_004370.6 | FACITS | decreased bone matrix deposition, disorganized and less polarized with disrupted cell-cell interactions, decreased connexin43 expression, and impaired gap junction function | Col12a1 | RDB20989 | RDB21002 |
| Collagen type 13 | α1 | 645 | 739 | NM_001368882.1 | membrane collagen | viable and fertile. Affected skeletal muscles showed abnormal myofibers with a fuzzy plasma membrane-basement membrane interphase along the muscle fiber and at the myotendinous junctions, disorganized myofilaments, and streaming of z-disks. | Col13a1 | - | - |
| Collagen type 14 | α1 | 1796 | 1794 | NM_021110.4 | FACITS | viable, however, mature skin exhibited altered mechanical properties | Col14a1 | - | RDB20950 |
| Collagen type 15 | α1 | 1388 | 1367 | NM_001855.5 | multiplexins | progressive histological changes characteristic for muscular diseases after 3 months of age, and they were more vulnerable than controls to exercise-induced muscle injury. Despite the antiangiogenic role of type XV collagen-derived endostatin, the development of the vasculature appeared normal in the null mice. | Col15a1 | RDB20808 | RDB20951 |
| Collagen type 16 | α1 | 1604 | 1580 | NM_001856.4 | FACITS | Early adult: decreased grip strength(Grip Strength); cataract(Eye Morphology); decreased circulating calcium level, decreased circulating total protein level(clinical chemistry) | Col16a1 | RDB20809 | RDB20952 |
| Collagen type 17 | α1 | 1497 | 1433 | NM_000494.4 | membrane collagen | most mice die within 2 weeks of birth | Col17a1 | RDB20990 | RDB20953 |
| Collagen type 18 | α1 | 1516 | 1315 | NM_130445.2 | multiplexins | Early adult: increased circulating triglyceride and decreased circulating glucose, HDL cholesterol level(Plasm Chemistry); increased circulating free fatty acids level(Clinical Chemistry); abnormal iris pigmentation, retina(Eye Morphology) etc. | Col18a1 | HKR041680 | RDB20954 |
| Collagen type 19 | α1 | 1142 | 1136 | NM_001858.6 | FACITS | lethargy (8 - 12 month old mutants display lethargy) | Col19a1 | RDB20811 | RDB21003 |
| Collagen type 20 | α1 | 1284 | 1320 | NM_020882.4 | FACITS | N.A. (not applicable) | Col20a1 | HGY030489 | RDB20955 |
| Collagen type 21 | α1 | 957 | NM_030820.4 | FACITS | N.A. (not applicable) | - | RDB20812 | Not exist | |
| Collagen type 22 | α1 | 1626 | 1613 | NM_152888.3 | FACITS | N.A. (not applicable) | Col22a1 | RDB20813 | RDB21004 |
| Collagen type 23 | α1 | 540 | 532 | NM_173465.4 | membrane collagen | N.A. (not applicable) | Col23a1 | RDB20814 | - |
| Collagen type 24 | α1 | 1714 | 1733 | NM_152890.7 | fibril | decreased body length | Col24a1 | RDB20815 | RDB20957 |
| Collagen type 25 | α1 | 642 | 666 | NM_198721.4 | membrane collagen | despite being viable at E18.5, mice die shortly after birth | Col25a1 | RDB20816 | - |
| Collagen type 26 | α1 | 439 | 440 | NM_001278563.3 | type XXVI collagen | N.A. (not applicable) | Col26a1 | RDB20817 | RDB21005 |
| Collagen type 27 | α1 | 1860 | 1845 | NM_032888.4 | fibril | embryonic growth retardation, abnormal placenta morphology | Col27a1 | RDB20818 | RDB20958 |
| Collagen type 28 | α1 | 1125 | 1141 | NM_001037763.3 | type XXVIII collagen | N.A. (not applicable) | Col28a1 | - | RDB20959 |
＊ 参考: Biochemistry of Collagens, Laminins and Elastin Structure, Function and Biomarkers (Second Edition)
[Reference: Biochemistry of Collagens, Laminins and Elastin Structure, Function and Biomarkers (Second Edition)]
細胞外マトリックス関連プラスミドリスト
[Extracellular Matrix-associated plasmids List]
マルチモーダルECMプロジェクトにて構築された、基底膜の構成タンパク質群および抗体データベースを公開いたします。ぜひ利活用ください。
We are pleased to release the basement membrane constitutive proteins and antibody database developed by the Multimodal ECM Project.
- KS(-)mFN1 (#RDB20960), mouse fibronectin1 cDNA
- KS(-)mTnc (#RDB21068), mouse tenascin C cDNA
- KS(-)mNcan (#RDB21067), mouse neurocan cDNA
- KS(-)mAcan (#RDB21066), mouse aggrecan cDNA
- KS(-)mCsn2 (#RDB21065), mouse casein beta cDNA
- KS(-)hPerlecan (#RDB20926), human Perlecan cDNA
- KS(-)mPerlecan (#RDB20927), mouse Perlecan cDNA
提供価格 1本 (1μg) あたり [Available price Per tube (1 μg)]
| 国内 Local (Japan) |
国外 Overseas |
|
|---|---|---|
| 非営利学術目的 Non-profit & Academic |
11,000円 (税込) 11,000 yen (incl. tax) |
22,000円 22,000 yen |
| 営利目的 For-profit |
22,000円 (税込) 22,000 yen (incl. tax) |
44,000円 44,000 yen |
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君はコラーゲンを知ってるか？[Do you know about collagen?]
-
ヒトのコラーゲンの中で、最もアミノ酸の数が多い(分子が大きい)ものはどれでしょう？ [Which human collagen has the largest number of amino acids (the largest molecule)?]
→ Collagen type 6のα鎖 (COL6A3)です: 3,177 a.a. [Collagen type VI alpha-3 chain (COL6A3), with 3,177 a.a.] -
最大コラーゲン遺伝子（分子）が大きいのは、ヒトとマウスのどちらでしょう？ [Which has the larger maximum collagen gene (molecule), humans or mice?]
→ マウスです。マウスのCollagen type 6のα鎖 (COL6A3)が3,284 a.a.あり、ヒトの3,177 a.a.を超えて全コラーゲン中トップの大きさを誇ります。[Mice. The mouse collagen type VI alpha-3 chain (COL6A3) consists of 3,284 a.a., surpassing the human version (3,177 a.a.) to rank as the largest among all collagens.] -
ヒトに存在してマウスに存在しないコラーゲン遺伝子は？マウスに存在してヒトに存在しないコラーゲン遺伝子は？ [Which collagen genes exist in humans but not in mice, and vice versa?]
→ ヒトに存在してマウスに存在しないコラーゲン遺伝子: Collagen type 21 α1です。マウスに存在してヒトに存在しないコラーゲン遺伝子: Collagen type 6 α4です。[In humans but not mice: Collagen type XXI alpha-1 (COL21A1). In mice but not humans: Collagen type VI alpha-4 (COL6A4).]
