Our office will be closed for the establishment anniversary on October 28(Mon.).
Thank you for your understanding.
Information to Residents of the European Economic Area (EEA)
News
Revision of Distribution Fees for the Bioresources (2019/09/18 T.M.)
Congratulations to Distinguished Professor Tasuku Honjo for the 2018 Nobel Prize! (2018/10/3 T.M.)
- The 2018 Nobel Prize in Physiology or Medicine for the discovery of cancer therapy by inhibition of negative immune regulation
Genetic resources regarding the prize are available (2018/10/3 T.M.)
- DNA resources deposited by Dr. Honjo
- Research tools to investigate the role of PD-1 in the mouse
- CTLA-4 clones
Dnaconda’s Recommendation
Novel vectors for efficient PCR cloning (2019/10/11 N.N.)
- Novel vectors for efficient cloning of PCR products by TA-cloning, blunt-end cloning and both cloning methods were recently developed and published in April of this year as described below, by Dr. Ken Motohashi of the Kyoto Sangyo University and are now available from us. We are looking forward to hearing your order![more…]
Elucidation of control system for regulating the morphology and orientation of cells in animal tissues. (2019/9/6 N.N.)
- The morphology and orientation of cells in tissues are determined by structural dynamics of the cytoskeleton and polarity of cells. It has been suggested that the structural dynamics of the cytoskeleton is induced during the exchange of signal transduction molecules. However, controlling the structural dynamics by the molecules remained to be elucidated.
[more…]
Fluorescent protein probe that can visualize the multiple inter-organelle contact sites in cells. (2019/3/29 N.N.)
- Divided GFP-fragment have a feature that they emit fluorescence again by reconstitution when each fragment is in close proximity.
[more…]
Resource Information
Function of transcription coactivator TAZ on the regulation of IL1B expression. (2019/5/13 T.M.)
- Involvement of YAP on the phenotype of malignant mesothelioma (MM) has been demonstrated. However, the function of TAZ, a paralog of YAP, in MM cells has not been clarified. [more…]
HMGA1 influences urokinase plasminogen activator system throught the regulation of PLAU and SERPINE1 gene. (2019/4/12 T.M.)
- HMGA1 is a oncofetal architectural transcription factor and has a role in controling genes in many of aspects of neoplastic transformation. [more…]
Quantitative evaluation of autophagic activity(2019/2/1 N.N.)
- In order to visualize autophagosome and observe autophagy, MAP1LC3 (LC3) fused with fluorescent proteins as a probe are used. However, the fluorescence of the probe attenuates during autophagy progresses and it is not suitable for quantitative assay of autophagy.
- Dr. Noboru Mizushima and his colleagues at the University of Tokyo developed a new probe that can expresses GFP-LC3 and RFP-LC3ΔG (lacking glycine (G) at the LC 3 end) simultaneously at equimolar amounts.[more…]
Akaluc luciferase providing brighter and red-shifted luminescence (2018/5/7 N.N.)
- Firefly bioluminescence systems have been commonly used as a imaging tool of biological phenomena. However, it have not been strong enough for imaging signals in deep tissues, because of low permeabilization of substrates. Recently, Dr. Atsushi Miyawaki and Dr. Satoshi Iwano of the RIKEN Center for Brain Science, and Dr. Shojiro Maki of the University of Electro-Communications developed a system of artificial bioluminescence AkaBLI that enables noninvasive signal observation in deep tissue of living animals. [more]
Stable ChIP-seq analysis using transcription factors tagged with biotinylated peptide tag. (2018/2/8 K.N.)
- ChIP-seq is a powerful method of comprehensive analysis of transcription factor (TF) binding sites. However, the bottle neck of ChIP-seq analysis is the quality of antibody, and thus many researchers have been given up. To overcome, authors constructed the vector expressing “biotinylated-tag” transcription factors. With this vector, you can get acceptable level of TF-DNA complex for ChIP-seq analysis by streptavidin-beads for any kinds of TF.[more]
Fluorescent probes for sphingomyelin and cholesterol lipid domains.(2018/02/06 N.N.)
- Lipid rafts are small lipid domains on the cell membrane and are thought to play an important role in signal transduction, endocytosis and more. Because of the lack of appropriate probes to label sphingomyelin and cholesterol individually, detailed analysis of lipid rafts was difficult.[more]
Announcements
- Revision of Distribution Fees for the Bioresources (2019/09/18 T.M.)
- Seeking a Principle Investigator (indefinite-term position), Gene Engineering Division (RIKEN BRC Gene Bank) (2019/07/26 T.M.)
- The extended national holidays (2019.04.15)
- Information to Residents of the European Economic Area (EEA) (2019.04.02 T.M.)
- New Director Appointed (2019.04.01)
- The Recommendation by Science Council of Japan: The treatment of Digital Sequence Information with respect to the Convention on Biological Diversity and the Nagoya Protocol (2018.02.21 T.M.)
- Holiday Closing Announcement (2017.12.19)
- It is great sorrow to announce of Dr. Kaoru Fukami-Kobayashi. (2017.08.05 T.M.)
- Announcement of bioresources developed by the CRISPR/Cas9 technology (2017.08.01 T.M.)
- A new BRC team, Drug-Discovery Cellular Basis Development Team has begun its R&D in Kyoto (2017.07.25 T.M.)
- Quality Examination and Information Release Policy on Our Bioresources (2014.09.01)
- What’s New. Recent information from RIKEN BioResource Research Center
