News
Congratulations to Distinguished Professor Tasuku Honjo for the 2018 Nobel Prize! (2018/10/3 T.M.)
- The 2018 Nobel Prize in Physiology or Medicine for the discovery of cancer therapy by inhibition of negative immune regulation
Genetic resources regarding the prize are available (2018/10/3 T.M.)
- DNA resources deposited by Dr. Honjo
- Research tools to investigate the role of PD-1 in the mouse
- CTLA-4 clones
Dnaconda’s Recommendation
Recombinant protein expression vector in fruit fly cell line, S2. (2019/2/22 T.Y.)
- The Drosophila culture cell (S2) is widely used for recombinant mammalian proteins, because of more chance to get active protein than bacteria and more chance to get free from interactors than mammalian culture cells. Here, authors introduce three additional tools (see below) for original pMT-PURO (cat# RDB08532) to aim more effective transfection and more stable transfectant during cultivation.
[more…]
Expression vector of optogenetics technique (2019/1/11 N.N.)
- New plasmids developed by Dr. Toru Ishizuka of Tohoku University are available.[more…]
mCherry expression vector with Cre/loxP system (2018/12/07 S.H.)
- Dr. Yumiko Hatanaka and her colleagues at Osaka University developed mCherry expression vector with Cre/loxP system.[more…]
Genetic resources to visualize organelle (2018/11/13 N.N.)
- We are providing genetic resources for visualization of 15 organelles such as mitochondria and nucleus. Each clone contains an organelle localization signal sequence fused with fluorescent proteins or epitope tags. Organelles can be detected by fluorescence or by detecting epitope tags with antibodies. [more]
Resource Information
Quantitative evaluation of autophagic activity(2019/2/1 N.N.)
- In order to visualize autophagosome and observe autophagy, MAP1LC3 (LC3) fused with fluorescent proteins as a probe are used. However, the fluorescence of the probe attenuates during autophagy progresses and it is not suitable for quantitative assay of autophagy.
- Dr. Noboru Mizushima and his colleagues at the University of Tokyo developed a new probe that can expresses GFP-LC3 and RFP-LC3ΔG (lacking glycine (G) at the LC 3 end) simultaneously at equimolar amounts.[more…]
Akaluc luciferase providing brighter and red-shifted luminescence (2018/5/7 N.N.)
- Firefly bioluminescence systems have been commonly used as a imaging tool of biological phenomena. However, it have not been strong enough for imaging signals in deep tissues, because of low permeabilization of substrates. Recently, Dr. Atsushi Miyawaki and Dr. Satoshi Iwano of the RIKEN Center for Brain Science, and Dr. Shojiro Maki of the University of Electro-Communications developed a system of artificial bioluminescence AkaBLI that enables noninvasive signal observation in deep tissue of living animals. [more]
Stable ChIP-seq analysis using transcription factors tagged with biotinylated peptide tag. (2018/2/8 K.N.)
- ChIP-seq is a powerful method of comprehensive analysis of transcription factor (TF) binding sites. However, the bottle neck of ChIP-seq analysis is the quality of antibody, and thus many researchers have been given up. To overcome, authors constructed the vector expressing “biotinylated-tag” transcription factors. With this vector, you can get acceptable level of TF-DNA complex for ChIP-seq analysis by streptavidin-beads for any kinds of TF.[more]
Fluorescent probes for sphingomyelin and cholesterol lipid domains.(2018/02/06 N.N.)
- Lipid rafts are small lipid domains on the cell membrane and are thought to play an important role in signal transduction, endocytosis and more. Because of the lack of appropriate probes to label sphingomyelin and cholesterol individually, detailed analysis of lipid rafts was difficult.[more]
Hedgehog signal analysis by the Gli recognition element reporter. (2018/2/5 T.M.)
- To elucidate roles of oxidative stress during the induction of osteoporosis and inhibition of hedgehog signaling, osteoblast differentiation in murine embryonic mesenchymal cell C3H10T1/2 was investigated with a focus on the interaction with mitogen-activated protein kinase (MAPKs) as possible mediator. To monitor Gli-mediated transcription activity in C3H10T1/2 cells under the oxidative stress and application of MAPK inhibitors, luciferase reporter having Gli-transcription factor recognition element was used and the inhibitory effect of JNK1 on Gli transcription was suggested. [more]
Announcements
- The Recommendation by Science Council of Japan: The treatment of Digital Sequence Information with respect to the Convention on Biological Diversity and the Nagoya Protocol (2018.02.21 T.M.)
- Holiday Closing Announcement (2017.12.19)
- It is great sorrow to announce of Dr. Kaoru Fukami-Kobayashi. (2017.08.05 T.M.)
- Announcement of bioresources developed by the CRISPR/Cas9 technology (2017.08.01 T.M.)
- A new BRC team, Drug-Discovery Cellular Basis Development Team has begun its R&D in Kyoto (2017.07.25 T.M.)
- Quality Examination and Information Release Policy on Our Bioresources (2014/09/01)
- What’s New. Recent information from RIKEN BioResource Research Center
