Quality control
We distribute
(1) plasmid and cosmid clones,
(2) BAC, YAC, P1, fosmid, cosmid from genomic clone libraries,
(3) recombinant adenoviruses,
(4) bacterial host,
(5) cDNA library,
(6) DNA clone set,
(7) microbial genome DNA, and
(8) mouse genome DNA.
Each product is tested in order to ensure the reproducibility of experimental results.
1. Plasmid and cosmid clones
Plasmids and cosmids are provided as DNA solution in TE buffer at ambient temperature. Plasmids and cosmids are amplified in transformed bacteria.
Information on nucleotide sequence, protein production and other properties obtained by the depositor/developer are shown in the web catalog.
Quality Test: Based on resource information provided by the depositor/developer, we perform the following tests:
- Performed:
- Growth and antibiotics resistance of the recombinant bacteria in the culture conditions specified by the depositor/developer
- Restriction enzyme mapping
- High Throughput Sequencing and/or Sanger sequencing around vector-insert junction and important portion(s) of vector
- Not Performed:
- Protein production
- Promoter activity, etc.
- Supplemental Tests:
- When any other tests for the quality of the bioresource are performed, the results are shown in the web catalog.
2.BAC, YAC, P1, fosmid, cosmid from genomic clone libraries
BAC, YAC, P1, fosmids, cosmids from genomic clone libraries are provided as recombinant bacteria.
Information on nucleotide sequence and other properties obtained by the depositor/developer are shown in the web catalog.
Quality Test: Based on resource information provided by the depositor/developer, we perform the following tests:
- Performed:
- Growth and antibiotics resistance of the recombinant bacteria in the culture conditions specified by the depositor/developer
- PCR to detect endo sequence registered in the DNA sequence database
- Not Performed:
- Restriction enzyme mapping
- Full length or terminal nucleotide sequencing, etc.
- Supplemental Tests:
- When any other tests for the quality of the bioresource are performed, the results are shown in the web catalog.
3. Recombinant adenoviruses
Recombinant adenovirus is amplified by infection to HEK293 cells. An aliquot of crude lysates of infected cells is provided.
Information on nucleotide sequence and other properties obtained by the depositor/developer are shown in the web catalog.
Quality Test: Based on resource information provided by the depositor/developer, we perform the following tests:
- Performed:
- Restriction enzyme mapping
- PCR amplification of E1 region to confirm that replication competent adenovirus (RCA) is under the detection level
- Nucleotide sequencing of vector-insert junction area and portions of vector sequences
- Not Performed:
- Full length nucleotide sequencing of virus
- Protein production, etc.
- Supplemental Tests:
- When any other tests for the quality of the bioresource are performed, the results are shown in the web catalog.
4.Bacterial host
Bacterial host is shipped as a stab-culture in a plastic vial at ambient temperature. Information on nutrient requirement and other properties obtained by the depositor/developer are shown in the web catalog.
Quality Test: Based on resource information provided by the depositor/developer, we perform the following tests:
- Performed:
- Nutrient requiring, growth and antibiotics resistance of the host bacteria in the culture conditions specified by the depositor/developer
- Not Performed:
- Genotyping, etc.
- Supplemental Tests:
- When any other tests for the quality of the bioresource are performed, the results are shown in the web catalog.
5. cDNA Library
cDNA libraries are amplified once. Information on number of independent clones, range and average of the insert size and other properties obtained by the depositor/developer are shown in the web catalog.
Quality Test: Based on resource information provided by the depositor/developer, we perform the following tests:
- Performed:
- Growth and antibiotics resistance of the recombinant bacteria in the culture conditions specified by the depositor/developer
- Not Performed:
- Number of independent clones
- Range and average of the insert size, etc.
- Supplemental Tests:
- When any other tests for the quality of the bioresource are performed, the results are shown in the web catalog.
6.DNA clone set
Master replica plate (the first copy from the originally deposited plate) is copied from the original set. Provided set is made from the master replica plates as a copy. Information on nucleotide sequence, protein production and other properties obtained by the depositor/developer are shown in the web catalog.
Quality Test: Based on resource information provided by the depositor/developer, we perform the following tests:
- Performed:
- Growth and antibiotics resistance of the recombinant bacteria in the culture conditions specified by the depositor/developer
- Restriction enzyme mapping of randomly picked up clones
- Nucleotide sequencing around vector-insert junction area of randomly picked up clones
- Not Performed:
- Restriction mapping of individual clones of entire set
- Full length or terminal nucleotide sequences of individual clones of the entire set
- Protein production, etc.
- Supplemental Tests:
- When any other tests for the quality of the bioresource are performed, the results are shown in the web catalog.
7. Microbial genome DNA
Microorganisms are cultured by the Microbe Division (JCM). Genomic DNA is extracted by the phenol-chloroform extraction by the Gene Engineering Division. Information on nucleotide sequence and other properties obtained by the depositor/developer are shown in the web catalog.
Quality Test: Based on resource information provided by the depositor/developer, we perform the following tests:
- Performed:
- Concentration (A260) and purity (A260/A280) of genomic DNA measured by a spectrophotometer
- Agarose gel electrophoresis to determine concentration (comparing to standard sample) and DNA fragmentation
- PCR amplification of 16S rRNA gene
- Nucleotide sequence of 16S rRNA gene
- Not Performed:
- Genetic back ground of strain by genetic marker, etc.
- Supplemental Tests:
- When any other tests for the quality of the bioresource are performed, the results are shown in the web catalog.
8.Mouse genome DNA
Mice for DNA extraction are maintained by the Experimental Animal Division. Genomic DNA is extracted by the phenol-chloroform extraction by the Gene Engineering Division. Information on any other properties obtained by the depositor/developer are shown in the web catalog.
Quality Test: Based on resource information provided by the depositor/developer, we perform the following tests:
- Performed:
- Concentration (A260) and purity (A260/A280) of genomic DNA measured by a spectrophotometer
- Agarose gel electrophoresis to determine concentration (comparing to standard sample) and DNA fragmentation
- Not Performed:
- Genetic back ground of a strain by genetic markers, etc.
- Supplemental Tests:
- When any other tests for the quality of the bioresource are performed, the results are shown in the web catalog.
(2014.09.22 T.M.)
2024.04.08
