Resources for Gene Expression

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Host and empty backbone vector

• E. coli host strain for producing recombinant proteins incorporating synthetic amino acids
• E. coli host strain for selective isotope labeling
• Thermostable host strain Bacillus stearothermophilus K1041 and vector
• Bifidobacterium longum vector

Empty backbone vector

• Cloning vector
• Mammalian cell
• Expression regulation
  • Cre-loxP and FLP-FRT system
  • Tet inducible system
• Insect cell
• Yeast Saccharomyces cerevisiae
• Yeast Schizosaccharomyces pombe
• Virus vector
  • AAV vector
  • Adenovirus
  • Lentivirus vector developed by Dr. Hiroyuki Miyoshi
  • Retrovirus vector

Expression in E. coli

• Aeropyrum pernix gene products
• Pyrococcus horikoshii gene products
• Sulfolobus tokodaii gene products
• Thermus thermophilus HB8 gene products
• Biomass saccharification enzyme

Expression in S. pombe

• S. pombe ORFeome clones

Experiment using Thermus thermophilus HB8

• T. thermophilus Gene Disluption plasmid

Expression in Mammalian Cells

• Human HLA class I
• Human SEREX cDNA

Control vector

• Luciferase, lacZ

Remarks
Please visit the Experimental Plant Division for more plant DNA clones such as pGWB vectors developed by Prof. Nakagawa Lab.

(2008.03.01 T.M.)

2019.11.11