Lab Manual


Lab Manual


Handling of genetic materials

  • Stab culture
    • Inoculate the bacteria on an agar plate containing appropriate selective antibiotics and/or nutritional supplements within a few days after you received the plastic vial of stab culture.
    • Pick up bacteria from the middle part of stab culture but not those from the surface of the stab which are not really healthy.
    • CAUTION: The vial should be placed in a refrigerator (4°C) but never in a freezer, otherwise spoiled.
  • BAC Related Information
  • Microbe Genomic DNA Preparation
  • Lentiviral vectors [link]

Sequencing & PCR primer

Protocol by using deposited matelials

Reference of Genetic Materials

  • List of references of genetic materials (promoters, plasmid vectors and so on).
  • List of RDB Clones in Publications
    RDB Clones in Publications is a list of scientific journal and patent application in which provided resources of RIKEN BRC DNA Bank were used. When you publish your research results in a scientific journal, please refer to the origin of materials that were provided from RIKEN BRC and used in your research subject. We appreciate your cooperation.

(MAN0000e 2011.01.12 T.M.)


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