Lentivirus Vector
- レンチウイルスベクターの提供申込み
- レンチウイルスベクター Plasmidリスト
- プロトコールとＱ＆Ａ
Plasmidリスト
Packaging Plasmids
| Cat. no. | Plasmid Name | Description |
|---|---|---|
| RDB04394 | pCAG-HIVgp | CAG promoter, HIV-1 gag and pol |
| RDB08256 | pCAG-HIVgp IN-D64V | CAG promoter, HIV-1 gag and pol, integrase mutant |
| RDB08943 | pCAG-HIVgp IN-D64E | CAG promoter, HIV-1 gag and pol, integrase mutant |
| RDB04393 | pCMV-VSV-G-RSV-Rev | CMV promoter, VSV-G, RSV promoter, Rev |
| RDB04392 | pCMV-VSV-G | CMV promoter, VSV-G |
| RDB08121 | pRSV-Rev | RSV promoter, Rev |
Vector Plasmids
cDNA expression – restriction enzyme cloning
| Cat. no. | Plasmid Name | Description |
|---|---|---|
| RDB04377 | CSII-CMV-MCS | CMV promoter |
| RDB04385 | CSII-CMV-MCS-IRES2-Bsd | CMV promoter, IRES, Bsd |
| RDB04383 | CSII-CMV-MCS-IRES2-Venus | CMV promoter, IRES, Venus |
| RDB08257 | CSIV-CMV-MCS-IRES2-Venus | CMV promoter, IRES, Venus, loxP |
| RDB07918 | CSII-CMV-MCS-IRES2-mRFP1 | CMV promoter, IRES, mRFP1 |
| RDB05963 | CS-CA-MCS | CAG promoter |
| RDB17751 | pLBS.CAG | CAG promoter |
| RDB19592 | CSII-CA-MCS-IRES-Puro | CAG promoter, IRES, Puro |
| RDB19593 | CSII-CA-MCS-IRES-Hyg | CAG promoter, IRES, Hyg |
| RDB04378 | CSII-EF-MCS | EF-1α promoter |
| RDB17829 | pLBS.EF | EF-1α promoter |
| RDB17867 | CSII-EF-IRES2-Bsd | EF-1α promoter, IRES, Bsd |
| RDB17866 | CSII-EF-IRES-GFP | EF-1α promoter, IRES, GFP |
| RDB04384 | CSII-EF-MCS-IRES2-Venus | EF-1α promoter, IRES, Venus |
| RDB17308 | CSIIR-EF-MCS-IRES2-Venus | EF-1α promoter, IRES, Venus |
| RDB07915 | CSII-EF-MCS-IRES2-hKO1 | EF-1α promoter, IRES, hKO1 |
| RDB07696 | CS-UbC-MCS-IRES2-Venus | UbC promoter, IRES, Venus |
| RDB08930 | CSIV-UbC-MCS-IRES2-Venus | UbC promoter, IRES, Venus, loxP |
cDNA expression – Gateway® cloning
| Cat. no. | Plasmid Name | Description |
|---|---|---|
| RDB04386 | CSII-CMV-RfA | CMV promoter, Gateway |
| RDB04388 | CSII-CMV-RfA-IRES2-Venus | CMV promoter, Gateway, IRES, Venus |
| RDB08070 | CSII-CMV-RfA-IRES2-hKO1 | CMV promoter, Gateway, IRES, hKO1 |
| RDB07894 | CS-CA-RfA-IRES2-Venus | CAG promoter, Gateway, IRES, Venus |
| RDB04387 | CSII-EF-RfA | EF-1α promoter, Gateway |
| RDB17869 | CSII-EF-IRES2-Bsd-RfA | EF-1α promoter, Gateway, IRES-Bsd |
| RDB12869 | CSII-EF-RfA-IRES-Puro | EF-1α promoter, Gateway, IRES, Puro |
| RDB17868 | CSII-EF-IRES-GFP-RfA | EF-1α promoter, Gateway, IRES-GFP |
| RDB04389 | CSII-EF-RfA-IRES2-Venus | EF-1α promoter, Gateway, IRES, Venus |
| RDB12870 | CSII-EF-RfA-IRES2-hKO1 | EF-1α promoter, Gateway, IRES, hKO1 |
| RDB08255 | CS-UbC-RfA-IRES2-Venus | UbC promoter, Gateway, IRES, Venus |
| RDB08350 | CS-UbC-RfA-IRES2-hKO1 | UbC promoter, Gateway, IRES, hKO1 |
Tet-inducible cDNA expression
| Cat. no. | Plasmid Name | Description |
|---|---|---|
| RDB12876 | CSIV-TRE-RfA-CMV-KT | Tet-responsive promoter, Gateway, CMV promoter, hKO1-2A-rtTA, loxP |
| RDB12878 | CSIV-TRE-RfA-UbC-KT | Tet-responsive promoter, Gateway, UbC promoter, hKO1-2A-rtTA, loxP |
| RDB12872 | CSIV-RfA-TRE-EF-KT | Tet-responsive promoter, Gateway, EF-1α promoter, hKO1-2A-rtTA, loxP |
Fluorescent protein gene expression
| Cat. no. | Plasmid Name | Description |
|---|---|---|
| RDB04379 | CS-CDF-CG-PRE | CMV promoter, EGFP |
| RDB05964 | CS-CA-GFP | CAG promoter, EGFP |
| RDB04380 | CS-CDF-EG-PRE | EF-1α promoter, EGFP |
| RDB12968 | CS-CDF-PGKG-PRE | PGK promoter, EGFP |
| RDB08363 | CS-CDF-UbCG-PRE | UbC promoter, EGFP |
| RDB15324 | CS-CMV-Bsd-IRES-GFP | CMV promoter, Bsd, IRES-GFP |
| RDB12868 | CSII-EF-GFP-IRES-Puro | EF-1α promoter, GFP, IRES-Puromycin resistance |
| RDB05553 | CSII-CMV-Venus | CMV promoter, Venus |
| RDB05552 | CSII-EF-Venus | EF-1α promoter, Venus |
| RDB05965 | CS-CA-Venus | CAG promoter, Venus |
| RDB07550 | CSII-CMV-Venus-NLS | CMV promoter, Venus-NLS |
| RDB07563 | CSII-EF-Venus-NLS | EF-1α promoter, Venus-NLS |
| RDB12867 | CS-EVBsd | EF-1α promoter promoter, Venus, Blasticidin resistance |
| RDB08118 | CSII-CMV-Venus-Mito-IRES2-Bsd | CMV promoter, Venus + mitochondrial targeting sequence, IRES, Blasticidin resistance |
| RDB06386 | CSII-CMV-mRFP1 | CMV promoter, mRFP1 |
| RDB06387 | CSII-EF-mRFP1 | EF-1α promoter, mRFP1 |
| RDB17830 | pLBS-mScarlet | EF-1α promoter, mScarlet |
| RDB17822 | pLBS.EF-iRFP | EF-1α promoter, iRFP |
Tet-inducible GFP expression
| Cat. no. | Plasmid Name | Description |
|---|---|---|
| RDB12875 | CSIV-TRE-GFP-CMV-KT | Tet-responsive promoter, EGFP, CMV promoter, hKO1-2A-rtTA, loxP |
| RDB12877 | CSIV-TRE-GFP-UbC-KT | Tet-responsive promoter, EGFP, UbC promoter, hKO1-2A-rtTA, loxP |
| RDB12871 | CSIV-GFP-TRE-EF-KT | Tet-responsive promoter, EGFP, EF-1α promoter, hKO1-2A-rtTA, loxP |
shRNA expression
| Cat. no. | Plasmid Name | Description |
|---|---|---|
| RDB04395 | pENTR4-H1 | Gateway entry vector, Human H1 promoter |
| RDB12882 | CSIV-RfA | Gateway, loxP |
| RDB04390 | CS-RfA-CG | Gateway, CMV promoter, EGFP |
| RDB12884 | CSIV-RfA-CG | Gateway, CMV promoter, EGFP, loxP |
| RDB04391 | CS-RfA-EG | Gateway, EF-1α promoter, EGFP |
| RDB12885 | CS-RfA-UbCG | Gateway, UbC promoter, EGFP |
| RDB05747 | CS-RfA-CMV-mRFP1 | Gateway, CMV promoter, mRFP1 |
| RDB05736 | CS-RfA-EF-mRFP1 | Gateway, EF-1α promoter, mRFP1 |
| RDB06090 | CS-RfA-EVBsd | Gateway, EF-1α promoter, Venus-2A-Blasticidin |
| RDB08240 | CS-CDF-CG-PRE-RfA | CMV promoter, EGFP, Gateway in the U3 region |
| RDB07722 | CS-CDF-EG-PRE-RfA | EF-1α promoter, EGFP, Gateway in the U3 region |
| RDB08492 | CSII-CMV-mRFP1-RfA | CMV promoter, mRFP1, Gateway in the U3 region |
| RDB12128 | CSII-EF-mRFP1-RfA | EF-1α promoter, mRFP1, Gateway in the U3 region |
Tet-inducible shRNA expression
| Cat. no. | Plasmid Name | Description |
|---|---|---|
| RDB07916 | pENTR4-H1tetOx1 | Gateway entry vector, Human H1 promoter, tet operator |
| RDB07917 | CS-RfA-ETBsd | Gateway, EF-1α promoter, TetR-2A-Blasticidin |
| RDB08020 | CS-RfA-ETV | Gateway, EF-1α promoter, TetR-2A-Venus |
| RDB08362 | CS-RfA-ETR | Gateway, EF-1α promoter, TetR-2A-mRFP1 |
For generating iPS cells (Human)
| Cat. no. | Plasmid Name | Description |
|---|---|---|
| RDB08323 | CSIV-CMV-hOct3/4-IRES2-Venus | CMV promoter, hOct3/4, IRES, Venus, loxP |
| RDB08324 | CSIV-CMV-hSox2-IRES2-Venus | CMV promoter, hSox2, IRES, Venus, loxP |
| RDB08325 | CSIV-CMV-hKlf4-IRES2-Venus | CMV promoter, hKlf4, IRES, Venus, loxP |
| RDB08326 | CSIV-CMV-hc-Myc-IRES2-Venus | CMV promoter, hc-Myc, IRES, Venus, loxP |
| RDB12903 | CSIV-CMV-hLin28-IRES2-Venus | CMV promoter, hLin28, IRES, Venus, loxP |
| RDB08327 | CSIV-CMV-SV40LT-IRES2-Venus | CMV promoter, SV40 LT, IRES, Venus, loxP |
| RDB12908 | CSII-EF-hOct3/4-IRES2-Venus | EF-1α promoter, hOct3/4, IRES, Venus |
| RDB12909 | CSII-EF-hSox2-IRES2-Venus | EF-1α promoter, hSox2, IRES, Venus |
| RDB12910 | CSII-EF-hKlf4-IRES2-Venus | EF-1α promoter, hKlf4, IRES, Venus |
| RDB12911 | CSII-EF-hc-Myc-IRES2-Venus | EF-1α promoter, hc-Myc, IRES, Venus |
| RDB12913 | CS-UbC-hOct3/4-IRES2-Venus | UbC promoter, hOct3/4, IRES, Venus |
| RDB12915 | CS-UbC-hSox2-IRES2-Venus | UbC promoter, hSox2, IRES, Venus |
| RDB12917 | CS-UbC-hKlf4-IRES2-Venus | UbC promoter, hKlf4, IRES, Venus |
| RDB12919 | CS-UbC-hc-Myc-IRES2-Venus | UbC promoter, hc-Myc, IRES, Venus |
| RDB12920 | CSIV-TRE-hOct3/4-CMV-KT | Tet-responsive promoter, hOct3/4, CMV promoter, hKO1-2A-rtTA, loxP |
| RDB12921 | CSIV-TRE-hSox2-CMV-KT | Tet-responsive promoter, hSox2, CMV promoter, hKO1-2A-rtTA, loxP |
| RDB12922 | CSIV-TRE-hKlf4-CMV-KT | Tet-responsive promoter, hKlf4, CMV promoter, hKO1-2A-rtTA, loxP |
| RDB12923 | CSIV-TRE-hc-Myc-CMV-KT | Tet-responsive promoter, hc-Myc, CMV promoter, hKO1-2A-rtTA, loxP |
| RDB12924 | CSIV-TRE-hLin28-CMV-KT | Tet-responsive promoter, hLin28, CMV promoter, hKO1-2A-rtTA, loxP |
| RDB12927 | CSIV-TRE-SV40LT-CMV-KT | Tet-responsive promoter, SV40 LT, CMV promoter, hKO1-2A-rtTA, loxP |
| RDB12946 | CS-hp53-shRNA-CG | Human H1 promoter, hp53-shRNA, CMV promoter, EGFP |
For generating iPS cells (Mouse)
| Cat. no. | Plasmid Name | Description |
|---|---|---|
| RDB08513 | CSII-CMV-mOct3/4-IRES2-Venus | CMV promoter, mOct3/4, IRES, Venus |
| RDB08514 | CSII-CMV-mSox2-IRES2-Venus | CMV promoter, mSox2, IRES, Venus |
| RDB08515 | CSII-CMV-mKlf4-IRES2-Venus | CMV promoter, mKlf4, IRES, Venus |
| RDB07992 | CSII-CMV-mc-Myc-IRES2-Venus | CMV promoter, mc-Myc, IRES, Venus |
| RDB12953 | CSIV-CMV-mc-Myc-IRES2-Venus | CMV promoter, mc-Myc, IRES, Venus, loxP |
| RDB12955 | CSII-EF-mOct3/4-IRES2-Venus | EF-1α promoter, mOct3/4, IRES, Venus |
| RDB12960 | CS-UbC-mOct3/4-IRES2-Venus | UbC promoter, mOct3/4, IRES, Venus |
| RDB12954 | CSIV-CMV-mOKS-IRES2-Venus | CMV promoter, mOct3/4-2A-mKlf4-2A-mSox2, IRES, Venus, loxP |
| RDB12961 | CSIV-TRE-mOct3/4-CMV-KT | Tet-responsive promoter, mOct3/4, CMV promoter, hKO1-2A-rtTA, loxP |
| RDB12962 | CSIV-TRE-mSox2-CMV-KT | Tet-responsive promoter, mSox2, CMV promoter, hKO1-2A-rtTA, loxP |
| RDB12963 | CSIV-TRE-mKlf4-CMV-KT | Tet-responsive promoter, mKlf4, CMV promoter, hKO1-2A-rtTA, loxP |
| RDB12964 | CSIV-TRE-mc-Myc-CMV-KT | Tet-responsive promoter, mc-Myc, CMV promoter, hKO1-2A-rtTA, loxP |
| RDB12966 | CS-mp53-shRNA-CG | Human H1 promoter, mp53-shRNA, CMV promoter, EGFP |
For establishing immortalized human erythroid progenitor cell lines
| Cat. no. | Plasmid Name | Description |
|---|---|---|
| RDB17719 | CSIV-TRE-HPV-E6/E7-UbC-KT | Tet-responsive promoter, HPV-E6/E7, UbC promoter, hKO1-2A-rtTA, loxP |
Fucci related lentivirus vector – developed by Dr. Miyawaki’s laboratory
Please visit Fucci.
(GRP0068j)
2024.09.24
