Empty Backbone Vector
| Cloning | Mammalian | Virus vector | Insect Cell | Yeast | Plant |
E. coli, Bifidobacterium, Bacillus のプラスミドベクターと宿主が利用可能です。
Cloning vector
- A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products.
Motohashi, K.
Sci. Rep. 9 (1): 6417 (2019). PubMed PMID 31015513.Catalog num. Clone name Description RDB17479 pCRT Plasmid vector for TA-cloning of PCR-products. RDB17481 pCRZero Plasmid vector for blunt-end cloning of PCR-products. RDB17480 CRZeroT Bifunctional plasmid vector for TA-cloning and blunt-end cloning of PCR-products. 
- Engineering FRET constructs using CFP and YFP.
Shimozono, S., Miyawaki, A.
Methods Cell Biol. 85: 381-393 (2008). PubMed PMID 18155471.Catalog num. Clone name Description RDB15126 pBS coupler 1 Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3]. RDB15127 pBS coupler 2 Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3]. RDB15128 pBS coupler 3 Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3]. RDB15129 pBS coupler 4 Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3]. RDB15130 pBS coupler 5 Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3]. RDB15131 pBS coupler 6 Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3]. -
Catalog num. Clone name Description RDB03699 pURV19-10 Cloning vector based on pUC19 with EcoRV site.
Mammalian expression
| Catalog num. | Clone name | Description |
|---|---|---|
| RDB01379 | pH2Rneo | Expression vector with simian virus 40 (SV40) early promoter |
| RDB01679 | pCALwL | A cassette plasmid to insert cDNA into downstream CAG promoter |
| RDB01862 | pCALNL5 | A cassette to introduce cDNA into mammalian chromosome |
| RDB14218 | pcDNA5-FLAG/HA/SBP | Expression vector of N-terminally FLAG/HA/SBP tagged proteins in mammalian cells. |
| Catalog num. Clone name Description |
Epitope tag | Antibiotic/visual marker | Promoter | Cloning | Antibiotics (bacteria) |
|---|---|---|---|---|---|
| RDB14213 pcDNA3.1-myc-GFP Expression vector of N-terminally myc-GFP-tagged proteins in mammalian cells. |
myc-GFP | Neo | CMV | Restriction enzyme | Amp |
| RDB14401 pCIneoEGFPC2 Plasmid vector for constructing GFP-tagged expression vector. |
EGFP | Neo | CMV | Restriction enzyme | Amp |
| RDB05956 pCMV_S-FLAG Expressing FLAG-tagged fusion protein, CMV promoter. |
FLAG | Neo | CMV | Restriction enzyme | Amp |
| RDB06083 pRSV_S-FLAG Expressing FLAG-tagged fusion protein, RSV promoter. |
FLAG | Neo | RSV | Restriction enzyme | Amp |
| RDB07396 pCMV_S-FLAGc Destination vector to generate eukaryotic expression vector, with FLAG-tag. |
FLAG | Neo | CMV | Gateway(R) | Amp |
| RDB07397 pRSV_s-FLAGc Destination vector to generate eukaryotic expression vector, with FLAG-tag. |
FLAG | Neo | RSV | Gateway(R) | Amp |
| RDB10461 pBRHCAG-cHA-pA Expression vector with CAG promoter, HA-tag, hygromycin selection (hph). |
HA | Hygro | CAG | Restriction enzyme | Amp |
| RDB02136 S-HA-pRc/CMV Expressing HA-tagged fusion protein, CMV promoter. |
HA | Neo | CMV | Restriction enzyme | Amp |
| RDB02139 D-HA-pRc/CMV Expressing a fusion protein tagged with duplicated HA, CMV promoter. |
2xHA | Neo | CMV | Restriction enzyme | Amp |
| RDB07105 pBRPyCAG-cHA-IN Expression vector with CAG promoter, HA-tag, Neoycin selection. |
HA | Neo | CAG | Restriction enzyme | Amp |
| RDB11391 pPyCAG-cHA-IN Expression vector with CAG promoter, HA-tag, Neomycin selection. |
HA | Neo | CAG | Restriction enzyme | Amp |
| RDB12114 pPyCAG-cHA-IpacflexEGFP Expression vector with CAG promoter, HA-tag, Puromycin selection. GFP marker. |
HA | Puro & EGFP | CAG | Restriction enzyme | Amp |
| RDB07101 pBRPyCAG-cHA-IP Expression vector with CAG promoter, HA-tag, Puromycin selection. |
HA | Puro | CAG | Restriction enzyme | Amp |
| RDB07100 pPyCAG-cHA-IP Expression vector with CAG promoter, HA-tag, Puromycin selection. |
HA | Puro | CAG | Restriction enzyme | Amp |
| RDB10479 pPyPGK-cHA-IP Expression vector with mouse PGK promoter, HA-tag, IRESpuro. |
HA | Puro | PGK | Restriction enzyme | Amp |
| RDB10460 pBRPyCAG-cHA-IP Expression vector with CAG promoter, HA-tag, Puromycin selection. |
HA | Puro | CAG | Restriction enzyme | Amp |
| RDB10435 pBR-CAG-cHA-pA Expression vector with CAG promoter, HA-tag. |
HA | n.a. | CAG | Restriction enzyme | Amp |
| RDB02140 S-Myc-pRc/CMV Expressing Myc-tagged fusion protein, CMV promoter. |
Myc | Neo | CMV | Restriction enzyme | Amp |
| RDB02141 D-Myc-pRc/CMV Expressing a fusion protein tagged with duplicated Myc epitopes, CMV promoter. |
2xMyc | Neo | CMV | Restriction enzyme | Amp |
| RDB02137 S-T7-pRc/CMV Expressing T7-tagged fusion protein, CMV promoter. |
T7 | Neo | CMV | Restriction enzyme | Amp |
| RDB02138 D-T7-pRc/CMV Expressing a fusion protein tagged with duplicated T7, CMV promoter. |
2xT7 | Neo | CMV | Restriction enzyme | Amp |
| RDB12113 pPyCAG-BstXI-ZeoP2AEGFP Expression vector with CAG promoter, HA-tag, Bleomycin (Zeo) selection. GFP marker. |
n.a. | Bleo(Zeo) & EGFP | CAG | Restriction enzyme(BstXI) | Amp |
| RDB09315 pPyCAG-IRESzeocinpA (+SVori) Expression vector with CAG promoter, IRES-zeo. |
n.a. | Bleo(Zeo) | CAG | Restriction enzyme (BstXI) | Amp |
| RDB09297 pZPCAGGS Expression vector with CAG promoter, Zeo marker. |
n.a. | Bleo(Zeo) | CAG | Restriction enzyme (BstXI) | Amp |
| RDB12107 pPyCAG-BstXI-IB Mammalian expression vector. Blasticidin resistance. |
n.a. | BSD | CAG | Restriction enzyme(BstXI) | Amp |
| RDB12112 pPyCAG-BstXI-IHisDflexEGFP Expression vector with CAG promoter, HA-tag, HisD selection. GFP marker. |
n.a. | HisD & EGFP | CAG | Restriction enzyme(BstXI) | Amp |
| RDB10322 pPyCAG-BstXI-IRSHisDpA Expression vector of IRES-HisD with CAG promoter. |
n.a. | HisD | CAG | Restriction enzyme (BstXI) | Amp |
| RDB12108 pPyCAG-BstXI-HygP2AEGFP Expression vector with CAG promoter, HA-tag, Hygromycin selection. GFP marker. |
n.a. | Hygro & EGFP | CAG | Restriction enzyme(BstXI) | Amp |
| RDB09233 pPyCAG-BstXI-IREShygropA Expression vector of CAG promoter, IRES-hygromycin resistance. |
n.a. | Hygro | CAG | Restriction enzyme(BstXI) | Amp |
| RDB09694 pCAGGSneo-delta-EcoRI Expression vector with CAG promoter and b-globin poly(A) (with SV40 ori), neo marker. |
n.a. | Neo | CAG | Restriction enzyme | Amp |
| RDB07099 pPyCAG-BstXI-IP Mammalian expression vector. Blasticidin resistance. |
n.a. | Puro | CAG | Restriction enzyme(BstXI) | Amp |
| RDB01968 pBMSA Expression vector, inducible MMTV-LTR promoter. |
n.a. | n.a. | MMTV-LTR | Restriction enzyme (XbaI) | Amp |
| RDB08938 pCAGGS Mammalian expression vector with CAG promoter. |
n.a. | n.a. | CAG | Restriction enzyme | Amp |
| RDB07939 pEF-BOS Mammalian expression vector with human EF-1a. |
n.a. | n.a. | EF-1 alpha | Restriction enzyme | Amp |
| RDB07940 pEF-BOS-EX Mammalian expression vector with human EF-1a. |
n.a. | n.a. | EF-1 alpha | Restriction enzyme | Amp |
| RDB14136 pIDT-SMART(C-TSC) Mammalian xpression vector with the improved high expression cassette C-TSC. |
n.a. | n.a. | CMV-TSC | Restriction enzyme | Kan |
Flurescent protein expression
| Catalog num. Clone name Description |
Antibiotic/visual marker | Promoter | Antibiotics (bacteria) |
|---|---|---|---|
| RDB10118 pmPGKpacECFPpA Expression vector of pac-ECFP with mPGK promoter. |
Puro & ECFP | CAG | Amp |
| RDB06071 pCMFlag_EGFP Expression vector of GFP, with FLAG-tag, control vector. |
FLAG-EGFP | CMV | Amp |
| RDB07071 pdxEFFlag_EGFPit2 Expression vector of EGFP. |
FLAG-EGFP | EF-1 alpha | Amp |
| RDB07073 pdxEFFlag_EGFPit2 Expression vector of EGFP. |
FLAG-EGFP | EF-1 alpha | Amp |
| RDB07631 pCAEGFP Expression vector of GFP driven by CA promoter. |
EGFP | CAG | Amp |
| RDB08525 pPyCAG-EGFP-IP Expression clone of GFP under the control of CAG promoter, puromycin resistance. |
Puro & EGFP | CAG | Amp |
| RDB08526 pPyCAG-EGFP-IZ Expression clone of GFP under the control of CAG promoter, zeocin resistance. |
Bleo(Zeo) & EGFP | CAG | Amp |
| RDB08527 pPyCAG-EGFP-ID Expression clone of GFP under the control of CAG promoter, HisD resistance. |
HisD & EGFP | CAG | Amp |
| RDB08528 pPyCAG-EGFP-IB Expression clone of GFP under the control of CAG promoter, Blasticidin resistance. |
BSD & EGFP | CAG | Amp |
| RDB08529 pBRPyCAG-EGFP-IH Expression clone of GFP under the control of CAG promoter, hygromycin resistance |
Hygro & EGFP | CAG | Amp |
| RDB08530 pBRPyCAG-EGFP-IN Expression clone of GFP under the control of CAG promoter, neomycin resistance |
Neo & EGFP | CAG | Amp |
| RDB03006 pCAEGFP Expression vectoe of jellyfish GFP |
EGFP | CAG | Amp |
| RDB03956 pCAEGFP Expression vector of EGFP. |
EGFP | CAG | Amp |
Vector for marker knock-in (genome editing)
- Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors.
Natsume, T., Kiyomitsu, T., Saga, Y., Kanemaki, M.T.
Cell Rep. 15 (1): 210-218 (2016). PubMed PMID 27052166.Selection marker Attached tag at the N-terminus mClover mCherry2 S tag-3FLAG Hygro pMK376 pMK377 pMK375 Selection marker Attached tag at the C-terminus mClover mCherry2 S tag-3FLAG Neo pMK277 pMK280 pMK283 Hygro pMK278 pMK281 pMK284 BSD pMK388 pMK389 pMK390 Bsr pMK279 pMK282 pMK285 Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use these clones.
組換えウイルス構築用
Adenovirus
- RIKEN BRC DNA Bank provides hundreds of shuttle vectors to generate recombinant adenovirus. We also provide vectors to construct shuttle vector for recombinant adenovirus.
- A list of Empty vectors for construction of recombinant adenovirus [link] is available
Retrovirus
| RDB no. | Antibiotic/visual marker | Promoter | Cloning | Antibiotics (bacteria) |
|---|---|---|---|---|
| RDB01699 pRx nZ ires Neo Nls (nuclear localization signal), lacZ, ires, neo |
lacZ-Neo | CMV-retroviral LTR | n.a. | Amp |
| RDB03985 pRxhrGFP Retroviral vector of a humanized recombinant GFP, hrGFP. |
GFP | CMV-retroviral LTR | Restriction enzyme | Amp |
| RDB03043 pMNSM Retrovirus expression vector in eukaryotic cells. |
Neo | Retroviral LTR | Restriction enzyme | Amp |
AAV
| Catalog num. | Clone name | Description |
|---|---|---|
| RDB15110 | pAAV2-DEST(f) | Gateway(R) destination vector of AAV2 [forword]. |
| RDB15111 | pAAV2-DEST(r) | Gateway(R) destination vector of AAV2 [reverse]. |
昆虫培養細胞利用
| Catalog num. Clone name Description |
Epitope tag | Antibiotic/visual marker | Cloning | Promoter | Bacterial resistance |
|---|---|---|---|---|---|
| RDB08532 pMT-PURO Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells. |
His-V5 | Puro | Restriction enzyme | MT | Amp |
| RDB12152 pMT-PURO2 Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells |
His-V5 | Puro | Restriction enzyme | MT | Amp |
| RDB09004 pMT-PURO2G Metametallothionein promoter expression vector with EGFP marker for Schneider 2 (S2) Drosophila cells |
His-V5 | Puro & EGFP | Restriction enzyme | MT | Amp |
| RDB09005 pMT-PURO2R Metametallothionein promoter expression vector with DsRED2 marker for Schneider 2 (S2) Drosophila cells |
His-V5 | Puro& DsRed | Restriction enzyme | MT | Amp |
| RDB08531 pCoPURO Copia promoter expression vector of puromycin resistant gene for Schneider 2 (S2) Drosophila cells. |
n.a. | Puro | n.a. | copia | Amp |
| RDB08035 pUAST-GFPN-lacZ P-element mediated gene transfer vector of GFP-N and lacZ. |
n.a. | GFP-lacZ | n.a. | Gal4-UAS | Amp |
酵母利用
植物材料利用
| Catalog num. | Clone name | Description |
|---|---|---|
| RDB02933 | pBAc3 | pBAc3 enhancer trap vector in rice. |
| RDB08246 | pCIYVV-Pst/CP | Plant expression vector constructed from clover yellow vein virus. |
Please visit the Experimental Plant Division for more plant DNA clones such as pGWB vectors developed by Prof. Nakagawa Lab.
(GRP0048j, 2010.04.24 T.M.)
2019.11.02