Empty Backbone Vector
| Cloning | Mammalian | Virus vector | Insect Cell | Yeast | Plant |
E. coli, Bifidobacterium, Bacillus のプラスミドベクターと宿主が利用可能です。
Cloning vector
- Molecular engineering of a PheS counterselection marker for improved operating efficiency in Escherichia coli.
Miyazaki, K.
Biotechniques 58 (2): 86-88 (2015). PubMed PMID 25652032.Catalog# Resource name Description RDB17947 pUC18K_ePAG2 Cloning vector harboring counterselection marker Escherichia coli phenylalanyl-tRNA synthetase a-subunit (ePheS). RDB17948 pUC18Z_ePAG2 Cloning vector harboring counterselection marker Escherichia coli phenylalanyl-tRNA synthetase a-subunit (ePheS). RDB18372 pUC18K_mPAG2 Cloning vector harboring PheS counterselection marker. Kanamycin resistance. RDB18373 pUC18Z_mPAG2 Cloning vector harboring PheS counterselection marker. Zeocin resistance. RDB18374 pUC18C_mPAG2 Cloning vector harboring PheS counterselection marker. Chloramphenicol resistance. - A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products.
Motohashi, K.
Sci. Rep. 9 (1): 6417 (2019). PubMed PMID 31015513.Catalog num. Clone name Description RDB17479 pCRT Plasmid vector for TA-cloning of PCR-products. RDB17481 pCRZero Plasmid vector for blunt-end cloning of PCR-products. RDB17480 pCRZeroT Bifunctional plasmid vector for TA-cloning and blunt-end cloning of PCR-products. - Engineering FRET constructs using CFP and YFP.
Shimozono, S., Miyawaki, A.
Methods Cell Biol. 85: 381-393 (2008). PubMed PMID 18155471.Catalog num. Clone name Description RDB15126 pBS coupler 1 Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3]. RDB15127 pBS coupler 2 Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3]. RDB15128 pBS coupler 3 Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3]. RDB15129 pBS coupler 4 Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3]. RDB15130 pBS coupler 5 Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3]. RDB15131 pBS coupler 6 Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3]. -
Catalog num. Clone name Description RDB03699 pURV19-10 Cloning vector based on pUC19 with EcoRV site.
Cloning vector
- Highly efficient induction of primate iPS cells by combining RNA transfection and chemical compounds.
Watanabe, T., Yamazaki, S., Yoneda, N., Shinohara, H., Tomioka, I., Higuchi, Y., Yagoto, M., Ema, M., Suemizu, H., Kawai, K., Sasaki, E.
Genes Cells 24 (7): 473-484 (2019). PubMed PMID 31099158.Catalog num. Clone name Description RDB18330 phBG Plasmid vector for in-vitro transcrioption, having two copies of 3′ UTR DNA fragment of human hemoglobin subunit beta (HBB)
Mammalian expression
Catalog num. | Clone name | Description |
---|---|---|
RDB01379 | pH2Rneo | Expression vector with simian virus 40 (SV40) early promoter |
RDB01679 | pCALwL | A cassette plasmid to insert cDNA into downstream CAG promoter |
RDB01862 | pCALNL5 | A cassette to introduce cDNA into mammalian chromosome |
RDB14218 | pcDNA5-FLAG/HA/SBP | Expression vector of N-terminally FLAG/HA/SBP tagged proteins in mammalian cells. |
Catalog num. Clone name Description |
Epitope tag | Antibiotic/visual marker | Promoter | Cloning | Antibiotics (bacteria) |
---|---|---|---|---|---|
RDB14213 pcDNA3.1-myc-GFP Expression vector of N-terminally myc-GFP-tagged proteins in mammalian cells. |
myc-GFP | Neo | CMV | Restriction enzyme | Amp |
RDB14401 pCIneoEGFPC2 Plasmid vector for constructing GFP-tagged expression vector. |
EGFP | Neo | CMV | Restriction enzyme | Amp |
RDB05956 pCMV_S-FLAG Expressing FLAG-tagged fusion protein, CMV promoter. |
FLAG | Neo | CMV | Restriction enzyme | Amp |
RDB06083 pRSV_S-FLAG Expressing FLAG-tagged fusion protein, RSV promoter. |
FLAG | Neo | RSV | Restriction enzyme | Amp |
RDB07396 pCMV_S-FLAGc Destination vector to generate eukaryotic expression vector, with FLAG-tag. |
FLAG | Neo | CMV | Gateway(R) | Amp |
RDB07397 pRSV_s-FLAGc Destination vector to generate eukaryotic expression vector, with FLAG-tag. |
FLAG | Neo | RSV | Gateway(R) | Amp |
RDB10461 pBRHCAG-cHA-pA Expression vector with CAG promoter, HA-tag, hygromycin selection (hph). |
HA | Hygro | CAG | Restriction enzyme | Amp |
RDB02136 S-HA-pRc/CMV Expressing HA-tagged fusion protein, CMV promoter. |
HA | Neo | CMV | Restriction enzyme | Amp |
RDB02139 D-HA-pRc/CMV Expressing a fusion protein tagged with duplicated HA, CMV promoter. |
2xHA | Neo | CMV | Restriction enzyme | Amp |
RDB07105 pBRPyCAG-cHA-IN Expression vector with CAG promoter, HA-tag, Neoycin selection. |
HA | Neo | CAG | Restriction enzyme | Amp |
RDB11391 pPyCAG-cHA-IN Expression vector with CAG promoter, HA-tag, Neomycin selection. |
HA | Neo | CAG | Restriction enzyme | Amp |
RDB12114 pPyCAG-cHA-IpacflexEGFP Expression vector with CAG promoter, HA-tag, Puromycin selection. GFP marker. |
HA | Puro & EGFP | CAG | Restriction enzyme | Amp |
RDB07101 pBRPyCAG-cHA-IP Expression vector with CAG promoter, HA-tag, Puromycin selection. |
HA | Puro | CAG | Restriction enzyme | Amp |
RDB07100 pPyCAG-cHA-IP Expression vector with CAG promoter, HA-tag, Puromycin selection. |
HA | Puro | CAG | Restriction enzyme | Amp |
RDB10479 pPyPGK-cHA-IP Expression vector with mouse PGK promoter, HA-tag, IRESpuro. |
HA | Puro | PGK | Restriction enzyme | Amp |
RDB10460 pBR-PyCAG-cHA-IP SVOri Expression vector with CAG promoter, HA-tag, Puromycin selection. |
HA | Puro | CAG | Restriction enzyme | Amp |
RDB10435 pBR-CAG-cHA-pA Expression vector with CAG promoter, HA-tag. |
HA | n.a. | CAG | Restriction enzyme | Amp |
RDB02140 S-Myc-pRc/CMV Expressing Myc-tagged fusion protein, CMV promoter. |
Myc | Neo | CMV | Restriction enzyme | Amp |
RDB02141 D-Myc-pRc/CMV Expressing a fusion protein tagged with duplicated Myc epitopes, CMV promoter. |
2xMyc | Neo | CMV | Restriction enzyme | Amp |
RDB02137 S-T7-pRc/CMV Expressing T7-tagged fusion protein, CMV promoter. |
T7 | Neo | CMV | Restriction enzyme | Amp |
RDB02138 D-T7-pRc/CMV Expressing a fusion protein tagged with duplicated T7, CMV promoter. |
2xT7 | Neo | CMV | Restriction enzyme | Amp |
RDB12113 pPyCAG-BstXI-ZeoP2AEGFP Expression vector with CAG promoter, HA-tag, Bleomycin (Zeo) selection. GFP marker. |
n.a. | Bleo(Zeo) & EGFP | CAG | Restriction enzyme(BstXI) | Amp |
RDB09315 pPyCAG-IRESzeocinpA (+SVori) Expression vector with CAG promoter, IRES-zeo. |
n.a. | Bleo(Zeo) | CAG | Restriction enzyme (BstXI) | Amp |
RDB09297 pZPCAGGS Expression vector with CAG promoter, Zeo marker. |
n.a. | Bleo(Zeo) | CAG | Restriction enzyme (BstXI) | Amp |
RDB12107 pPyCAG-BstXI-IB Mammalian expression vector. Blasticidin resistance. |
n.a. | BSD | CAG | Restriction enzyme(BstXI) | Amp |
RDB12112 pPyCAG-BstXI-IHisDflexEGFP Expression vector with CAG promoter, HA-tag, HisD selection. GFP marker. |
n.a. | HisD & EGFP | CAG | Restriction enzyme(BstXI) | Amp |
RDB10322 pPyCAG-BstXI-IRSHisDpA Expression vector of IRES-HisD with CAG promoter. |
n.a. | HisD | CAG | Restriction enzyme (BstXI) | Amp |
RDB12108 pPyCAG-BstXI-HygP2AEGFP Expression vector with CAG promoter, HA-tag, Hygromycin selection. GFP marker. |
n.a. | Hygro & EGFP | CAG | Restriction enzyme(BstXI) | Amp |
RDB09233 pPyCAG-BstXI-IREShygropA Expression vector of CAG promoter, IRES-hygromycin resistance. |
n.a. | Hygro | CAG | Restriction enzyme(BstXI) | Amp |
RDB09694 pCAGGSneo-delta-EcoRI Expression vector with CAG promoter and b-globin poly(A) (with SV40 ori), neo marker. |
n.a. | Neo | CAG | Restriction enzyme | Amp |
RDB07099 pPyCAG-BstXI-IP Mammalian expression vector. Blasticidin resistance. |
n.a. | Puro | CAG | Restriction enzyme(BstXI) | Amp |
RDB01968 pBMSA Expression vector, inducible MMTV-LTR promoter. |
n.a. | n.a. | MMTV-LTR | Restriction enzyme (XbaI) | Amp |
RDB08938 pCAGGS Mammalian expression vector with CAG promoter. |
n.a. | n.a. | CAG | Restriction enzyme | Amp |
RDB07939 pEF-BOS Mammalian expression vector with human EF-1a. |
n.a. | n.a. | EF-1 alpha | Restriction enzyme | Amp |
RDB07940 pEF-BOS-EX Mammalian expression vector with human EF-1a. |
n.a. | n.a. | EF-1 alpha | Restriction enzyme | Amp |
RDB14136 pIDT-SMART(C-TSC) Mammalian xpression vector with the improved high expression cassette C-TSC. |
n.a. | n.a. | CMV-TSC | Restriction enzyme | Kan |
Flurescent protein expression
Catalog num. Clone name Description |
Antibiotic/visual marker | Promoter | Antibiotics (bacteria) |
---|---|---|---|
RDB10118 pmPGKpacECFPpA Expression vector of pac-ECFP with mPGK promoter. |
Puro & ECFP | CAG | Amp |
RDB06071 pCMFlag_EGFP Expression vector of GFP, with FLAG-tag, control vector. |
FLAG-EGFP | CMV | Amp |
RDB07071 pdxEFFlag_EGFPit2 Expression vector of EGFP. |
FLAG-EGFP | EF-1 alpha | Amp |
RDB07073 pdxEFFlag_EGFPit2 Expression vector of EGFP. |
FLAG-EGFP | EF-1 alpha | Amp |
RDB07631 pCAEGFP Expression vector of GFP driven by CA promoter. |
EGFP | CAG | Amp |
RDB08525 pPyCAG-EGFP-IP Expression clone of GFP under the control of CAG promoter, puromycin resistance. |
Puro & EGFP | CAG | Amp |
RDB08526 pPyCAG-EGFP-IZ Expression clone of GFP under the control of CAG promoter, zeocin resistance. |
Bleo(Zeo) & EGFP | CAG | Amp |
RDB08527 pPyCAG-EGFP-ID Expression clone of GFP under the control of CAG promoter, HisD resistance. |
HisD & EGFP | CAG | Amp |
RDB08528 pPyCAG-EGFP-IB Expression clone of GFP under the control of CAG promoter, Blasticidin resistance. |
BSD & EGFP | CAG | Amp |
RDB08529 pBRPyCAG-EGFP-IH Expression clone of GFP under the control of CAG promoter, hygromycin resistance |
Hygro & EGFP | CAG | Amp |
RDB08530 pBRPyCAG-EGFP-IN Expression clone of GFP under the control of CAG promoter, neomycin resistance |
Neo & EGFP | CAG | Amp |
RDB03006 pCAEGFP Expression vectoe of jellyfish GFP |
EGFP | CAG | Amp |
RDB03956 pCAEGFP Expression vector of EGFP. |
EGFP | CAG | Amp |
Vector for marker knock-in (genome editing)
- Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors.
Natsume, T., Kiyomitsu, T., Saga, Y., Kanemaki, M.T.
Cell Rep. 15 (1): 210-218 (2016). PubMed PMID 27052166.Selection marker Attached tag at the N-terminus mClover mCherry2 S tag-3FLAG Hygro pMK376 pMK377 pMK375 Selection marker Attached tag at the C-terminus mClover mCherry2 S tag-3FLAG Neo pMK277 pMK280 pMK283 Hygro pMK278 pMK281 pMK284 BSD pMK388 pMK389 pMK390 Bsr pMK279 pMK282 pMK285 Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use these clones.
組換えウイルス構築用
Adenovirus
- RIKEN BRC DNA Bank provides hundreds of shuttle vectors to generate recombinant adenovirus. We also provide vectors to construct shuttle vector for recombinant adenovirus.
- A list of Empty vectors for construction of recombinant adenovirus [link] is available

Retrovirus
RDB no. | Antibiotic/visual marker | Promoter | Cloning | Antibiotics (bacteria) |
---|---|---|---|---|
RDB01699 pRx nZ ires Neo Nls (nuclear localization signal), lacZ, ires, neo |
lacZ-Neo | CMV-retroviral LTR | n.a. | Amp |
RDB03985 pRxhrGFP Retroviral vector of a humanized recombinant GFP, hrGFP. |
GFP | CMV-retroviral LTR | Restriction enzyme | Amp |
RDB03043 pMNSM Retrovirus expression vector in eukaryotic cells. |
Neo | Retroviral LTR | Restriction enzyme | Amp |
AAV
Catalog num. | Clone name | Description |
---|---|---|
RDB15110 | pAAV2-DEST(f) | Gateway(R) destination vector of AAV2 [forword]. |
RDB15111 | pAAV2-DEST(r) | Gateway(R) destination vector of AAV2 [reverse]. |
昆虫培養細胞利用
Catalog num. Clone name Description |
Epitope tag | Antibiotic/visual marker | Cloning | Promoter | Bacterial resistance |
---|---|---|---|---|---|
RDB08532 pMT-PURO Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells. |
His-V5 | Puro | Restriction enzyme | MT | Amp |
RDB12152 pMT-PURO2 Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells |
His-V5 | Puro | Restriction enzyme | MT | Amp |
RDB09002 pMAK17 Expression clone of EGFP under the control of tet inducible promoter. Puromycin serectable. |
– | Puro & DsRed2 | – | tight TRE | Amp |
RDB09003 pMAK42 Expression vector under the control of tet inducible promoter. Puromycin serectable. |
– | Puro & DsRed2 | Restriction enzyme | tight TRE | Amp |
RDB09004 pMT-PURO2G Metametallothionein promoter expression vector with EGFP marker for Schneider 2 (S2) Drosophila cells |
His-V5 | Puro & EGFP | Restriction enzyme | MT | Amp |
RDB09005 pMT-PURO2R Metametallothionein promoter expression vector with DsRED2 marker for Schneider 2 (S2) Drosophila cells |
His-V5 | Puro& DsRed | Restriction enzyme | MT | Amp |
RDB08531 pCoPURO Copia promoter expression vector of puromycin resistant gene for Schneider 2 (S2) Drosophila cells. |
n.a. | Puro | n.a. | copia | Amp |
RDB08035 pUAST-GFPN-lacZ P-element mediated gene transfer vector of GFP-N and lacZ. |
n.a. | GFP-lacZ | n.a. | Gal4-UAS | Amp |
酵母利用
植物材料利用
Catalog num. | Clone name | Description |
---|---|---|
RDB02933 | pBAc3 | pBAc3 enhancer trap vector in rice. |
RDB08246 | pCIYVV-Pst/CP | Plant expression vector constructed from clover yellow vein virus. |
Please visit the Experimental Plant Division for more plant DNA clones such as pGWB vectors developed by Prof. Nakagawa Lab.
(GRP0048j, 2010.04.24 T.M.)
2021.01.06