Empty Backbone Vector
| Mammalian | Virus vector | Insect Cell | Yeast | Plant |
| E. coli | Bifidobacterium | Bacillus |
Mammalian expression vector (empty backbone)
| Catalog num. Clone name Description |
Epitope tag | Antibiotic/visual marker | Promoter | Cloning | Antibiotics (bacteria) |
|---|---|---|---|---|---|
| RDB14213 pcDNA3.1-myc-GFP Expression vector of N-terminally myc-GFP-tagged proteins in mammalian cells. |
myc-GFP | Neo | CMV | Restriction enzyme | Amp |
| RDB14401 pCIneoEGFPC2 Plasmid vector for constructing GFP-tagged expression vector. |
EGFP | Neo | CMV | Restriction enzyme | Amp |
| RDB05956 pCMV_S-FLAG Expressing FLAG-tagged fusion protein, CMV promoter. |
FLAG | Neo | CMV | Restriction enzyme | Amp |
| RDB06083 pRSV_S-FLAG Expressing FLAG-tagged fusion protein, RSV promoter. |
FLAG | Neo | RSV | Restriction enzyme | Amp |
| RDB07396 pCMV_S-FLAGc Destination vector to generate eukaryotic expression vector, with FLAG-tag. |
FLAG | Neo | CMV | Gateway(R) | Amp |
| RDB07397 pRSV_s-FLAGc Destination vector to generate eukaryotic expression vector, with FLAG-tag. |
FLAG | Neo | RSV | Gateway(R) | Amp |
| RDB10461 pBRHCAG-cHA-pA Expression vector with CAG promoter, HA-tag, hygromycin selection (hph). |
HA | Hygro | CAG | Restriction enzyme | Amp |
| RDB02136 S-HA-pRc/CMV Expressing HA-tagged fusion protein, CMV promoter. |
HA | Neo | CMV | Restriction enzyme | Amp |
| RDB02139 D-HA-pRc/CMV Expressing a fusion protein tagged with duplicated HA, CMV promoter. |
2xHA | Neo | CMV | Restriction enzyme | Amp |
| RDB07105 pBRPyCAG-cHA-IN Expression vector with CAG promoter, HA-tag, Neoycin selection. |
HA | Neo | CAG | Restriction enzyme | Amp |
| RDB11391 pPyCAG-cHA-IN Expression vector with CAG promoter, HA-tag, Neomycin selection. |
HA | Neo | CAG | Restriction enzyme | Amp |
| RDB12114 pPyCAG-cHA-IpacflexEGFP Expression vector with CAG promoter, HA-tag, Puromycin selection. GFP marker. |
HA | Puro & EGFP | CAG | Restriction enzyme | Amp |
| RDB07101 pBRPyCAG-cHA-IP Expression vector with CAG promoter, HA-tag, Puromycin selection. |
HA | Puro | CAG | Restriction enzyme | Amp |
| RDB07100 pPyCAG-cHA-IP Expression vector with CAG promoter, HA-tag, Puromycin selection. |
HA | Puro | CAG | Restriction enzyme | Amp |
| RDB10479 pPyPGK-cHA-IP Expression vector with mouse PGK promoter, HA-tag, IRESpuro. |
HA | Puro | PGK | Restriction enzyme | Amp |
| RDB10460 pBRPyCAG-cHA-IP Expression vector with CAG promoter, HA-tag, Puromycin selection. |
HA | Puro | CAG | Restriction enzyme | Amp |
| RDB10435 pBR-CAG-cHA-pA Expression vector with CAG promoter, HA-tag. |
HA | n.a. | CAG | Restriction enzyme | Amp |
| RDB02140 S-Myc-pRc/CMV Expressing Myc-tagged fusion protein, CMV promoter. |
Myc | Neo | CMV | Restriction enzyme | Amp |
| RDB02141 D-Myc-pRc/CMV Expressing a fusion protein tagged with duplicated Myc epitopes, CMV promoter. |
2xMyc | Neo | CMV | Restriction enzyme | Amp |
| RDB02137 S-T7-pRc/CMV Expressing T7-tagged fusion protein, CMV promoter. |
T7 | Neo | CMV | Restriction enzyme | Amp |
| RDB02138 D-T7-pRc/CMV Expressing a fusion protein tagged with duplicated T7, CMV promoter. |
2xT7 | Neo | CMV | Restriction enzyme | Amp |
| RDB12113 pPyCAG-BstXI-ZeoP2AEGFP Expression vector with CAG promoter, HA-tag, Bleomycin (Zeo) selection. GFP marker. |
n.a. | Bleo(Zeo) & EGFP | CAG | Restriction enzyme(BstXI) | Amp |
| RDB09315 pPyCAG-IRESzeocinpA (+SVori) Expression vector with CAG promoter, IRES-zeo. |
n.a. | Bleo(Zeo) | CAG | Restriction enzyme (BstXI) | Amp |
| RDB09297 pZPCAGGS Expression vector with CAG promoter, Zeo marker. |
n.a. | Bleo(Zeo) | CAG | Restriction enzyme (BstXI) | Amp |
| RDB12107 pPyCAG-BstXI-IB Mammalian expression vector. Blasticidin resistance. |
n.a. | BSD | CAG | Restriction enzyme(BstXI) | Amp |
| RDB12112 pPyCAG-BstXI-IHisDflexEGFP Expression vector with CAG promoter, HA-tag, HisD selection. GFP marker. |
n.a. | HisD & EGFP | CAG | Restriction enzyme(BstXI) | Amp |
| RDB10322 pPyCAG-BstXI-IRSHisDpA Expression vector of IRES-HisD with CAG promoter. |
n.a. | HisD | CAG | Restriction enzyme (BstXI) | Amp |
| RDB12108 pPyCAG-BstXI-HygP2AEGFP Expression vector with CAG promoter, HA-tag, Hygromycin selection. GFP marker. |
n.a. | Hygro & EGFP | CAG | Restriction enzyme(BstXI) | Amp |
| RDB09233 pPyCAG-BstXI-IREShygropA Expression vector of CAG promoter, IRES-hygromycin resistance. |
n.a. | Hygro | CAG | Restriction enzyme(BstXI) | Amp |
| RDB09694 pCAGGSneo-delta-EcoRI Expression vector with CAG promoter and b-globin poly(A) (with SV40 ori), neo marker. |
n.a. | Neo | CAG | Restriction enzyme | Amp |
| RDB07099 pPyCAG-BstXI-IP Mammalian expression vector. Blasticidin resistance. |
n.a. | Puro | CAG | Restriction enzyme(BstXI) | Amp |
| RDB01968 pBMSA Expression vector, inducible MMTV-LTR promoter. |
n.a. | n.a. | MMTV-LTR | Restriction enzyme (XbaI) | Amp |
| RDB08938 pCAGGS Mammalian expression vector with CAG promoter. |
n.a. | n.a. | CAG | Restriction enzyme | Amp |
| RDB07939 pEF-BOS Mammalian expression vector with human EF-1a. |
n.a. | n.a. | EF-1 alpha | Restriction enzyme | Amp |
| RDB07940 pEF-BOS-EX Mammalian expression vector with human EF-1a. |
n.a. | n.a. | EF-1 alpha | Restriction enzyme | Amp |
| RDB14136 pIDT-SMART(C-TSC) Mammalian xpression vector with the improved high expression cassette C-TSC. |
n.a. | n.a. | CMV-TSC | Restriction enzyme | Kan |
Flurescent protein expression
| Catalog num. Clone name Description |
Antibiotic/visual marker | Promoter | Antibiotics (bacteria) |
|---|---|---|---|
| RDB10118 pmPGKpacECFPpA Expression vector of pac-ECFP with mPGK promoter. |
Puro & ECFP | CAG | Amp |
| RDB06071 pCMFlag_EGFP Expression vector of GFP, with FLAG-tag, control vector. |
FLAG-EGFP | CMV | Amp |
| RDB07071 pdxEFFlag_EGFPit2 Expression vector of EGFP. |
FLAG-EGFP | EF-1 alpha | Amp |
| RDB07073 pdxEFFlag_EGFPit2 Expression vector of EGFP. |
FLAG-EGFP | EF-1 alpha | Amp |
| RDB07631 pCAEGFP Expression vector of GFP driven by CA promoter. |
EGFP | CAG | Amp |
| RDB08525 pPyCAG-EGFP-IP Expression clone of GFP under the control of CAG promoter, puromycin resistance. |
Puro & EGFP | CAG | Amp |
| RDB08526 pPyCAG-EGFP-IZ Expression clone of GFP under the control of CAG promoter, zeocin resistance. |
Bleo(Zeo) & EGFP | CAG | Amp |
| RDB08527 pPyCAG-EGFP-ID Expression clone of GFP under the control of CAG promoter, HisD resistance. |
HisD & EGFP | CAG | Amp |
| RDB08528 pPyCAG-EGFP-IB Expression clone of GFP under the control of CAG promoter, Blasticidin resistance. |
BSD & EGFP | CAG | Amp |
| RDB08529 pBRPyCAG-EGFP-IH Expression clone of GFP under the control of CAG promoter, hygromycin resistance |
Hygro & EGFP | CAG | Amp |
| RDB08530 pBRPyCAG-EGFP-IN Expression clone of GFP under the control of CAG promoter, neomycin resistance |
Neo & EGFP | CAG | Amp |
| RDB03006 pCAEGFP Expression vectoe of jellyfish GFP |
EGFP | CAG | Amp |
| RDB03956 pCAEGFP Expression vector of EGFP. |
EGFP | CAG | Amp |
Vector for marker knock-in (genome editing)
| Catalog num. Clone name Description |
Epitope tag | Antiviotics marker | Antibiotics (bacterial) |
|---|---|---|---|
| RDB13925 pMK282 (mCherry2-Bsr) A plasmid for construction of C-terminal mCherry tagging donors. |
mCherry2-Bsr | Bsr | Amp |
| RDB13924 pMK281 (mCherry2-Hygro) A plasmid for construction of C-terminal mCherry tagging donors. |
mCherry2-Hygro | Hygro | Amp |
| RDB13923 pMK280 (mCherry2-NeoR) A plasmid for construction of C-terminal mCherry tagging donors. |
mCherry2-NeoR | Neo | Amp |
| RDB13922 pMK279 (mClover-Bsr) A plasmid for construction of C-terminal mClover tagging donors. |
mClover-Bsr | Bsr | Amp |
| RDB13921 pMK278 (mClover-Hygro) A plasmid for construction of C-terminal mClover tagging donors. |
mClover-Hygro | Hygro | Amp |
| RDB13920 pMK277 (mClover-NeoR) A plasmid for construction of C-terminal mClover tagging donors. |
mClover-Neo | Neo | Amp |
| RDB13928 pMK285 (Stag-3FLAG-Bsr) A plasmid for construction of C-terminal Stag-3FLAG tagging donors. |
Stag-3FLAG-Bsr | Bsr | Amp |
| RDB13927 pMK284 (Stag-FLAG-Hygro) A plasmid for construction of C-terminal Stag-3FLAG tagging donors. |
Stag-FLAG-Hygro | Hygro | Amp |
| RDB13926 pMK283 (Stag-3FLAG-Neo) A plasmid for construction of C-terminal Stag-3FLAG tagging donors. |
Stag-3FLAG-Neo | Neo | Amp |
| Catalog num. | Clone name | Description |
|---|---|---|
| RDB01379 | pH2Rneo | Expression vector with simian virus 40 (SV40) early promoter |
| RDB01679 | pCALwL | A cassette plasmid to insert cDNA into downstream CAG promoter |
| RDB01862 | pCALNL5 | A cassette to introduce cDNA into mammalian chromosome |
組換えウイルス構築用
Adenovirus
- RIKEN BRC DNA Bank provides hundreds of shuttle vectors to generate recombinant adenovirus. We also provide vectors to construct shuttle vector for recombinant adenovirus.
- A list of Empty vectors for construction of recombinant adenovirus [link] is available
Retrovirus
| RDB no. | Antibiotic/visual marker | Promoter | Cloning | Antibiotics (bacteria) |
|---|---|---|---|---|
| RDB01699 pRx nZ ires Neo Nls (nuclear localization signal), lacZ, ires, neo |
lacZ-Neo | CMV-retroviral LTR | n.a. | Amp |
| RDB03985 pRxhrGFP Retroviral vector of a humanized recombinant GFP, hrGFP. |
GFP | CMV-retroviral LTR | Restriction enzyme | Amp |
| RDB03043 pMNSM Retrovirus expression vector in eukaryotic cells. |
Neo | Retroviral LTR | Restriction enzyme | Amp |
AAV
| Catalog num. | Clone name | Description |
|---|---|---|
| RDB15110 | pAAV2-DEST(f) | Gateway(R) destination vector of AAV2 [forword]. |
| RDB15111 | pAAV2-DEST(r) | Gateway(R) destination vector of AAV2 [reverse]. |
昆虫培養細胞利用
| Catalog num. Clone name Description |
Epitope tag | Antibiotic/visual marker | Cloning | Promoter | Bacterial resistance |
|---|---|---|---|---|---|
| RDB08532 pMT-PURO Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells. |
His-V5 | Puro | Restriction enzyme | MT | Amp |
| RDB12152 pMT-PURO2 Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells |
His-V5 | Puro | Restriction enzyme | MT | Amp |
| RDB09004 pMT-PURO2G Metametallothionein promoter expression vector with EGFP marker for Schneider 2 (S2) Drosophila cells |
His-V5 | Puro & EGFP | Restriction enzyme | MT | Amp |
| RDB09005 pMT-PURO2R Metametallothionein promoter expression vector with DsRED2 marker for Schneider 2 (S2) Drosophila cells |
His-V5 | Puro& DsRed | Restriction enzyme | MT | Amp |
| RDB08531 pCoPURO Copia promoter expression vector of puromycin resistant gene for Schneider 2 (S2) Drosophila cells. |
n.a. | Puro | n.a. | copia | Amp |
| RDB08035 pUAST-GFPN-lacZ P-element mediated gene transfer vector of GFP-N and lacZ. |
n.a. | GFP-lacZ | n.a. | Gal4-UAS | Amp |
酵母利用
植物材料利用
| Catalog num. | Clone name | Description |
|---|---|---|
| RDB02933 | pBAc3 | pBAc3 enhancer trap vector in rice. |
| RDB08246 | pCIYVV-Pst/CP | Plant expression vector constructed from clover yellow vein virus. |
培養微生物利用
E. coli利用
- Vectors
Catalog num. Clone name ori Registance (E. coli) Cloning Promoter Tag Amp Tet Cm lac T7 FLAG His GST RDB06084 pETEG5 pBR322 
Restriction Enzyme RDB06211 pT7Tet1 p15A Restriction Enzyme RDB06212 pT7Tet6 p15A Restriction Enzyme RDB07041 pETHFFc pBR322 GatewayR RDB07050 pETF pBR322 Restriction Enzyme RDB07051 pT7FCam p15A Restriction Enzyme Catalog num. Clone name Description RDB15466 pNK E. coli expression vector, PA1lacO-1 promoter, rrnBT1T2 terminator, p15A and pAM alpha1 origin. Catalog num. Clone name Description RDB01911 Lambda GEX5 Lambda phage cloning vector producing GST fusion protein.
Bifidobacterium longum vectors and bacterial host
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Catalog num. Resource name Description RDB15972 pTBR101-CM Bifidobacterium longum shuttle vector RDB15973 pBS423 delta repA Bifidobacterium longum shuttle vector RDB15974 pBS423 Bifidobacterium longum shuttle vector JCM 31944 105-A Host strain of Bifidobacterium longum 105-A available from JCM.