- 大腸菌の取扱い
- プラスミド、コスミド、BACクローン
- ゲノムDNA
- 哺乳動物培養細胞
- 組換えウイルス
- Technical notes
- 寄託された遺伝子材料を用いたプロトコール
- Reference of Genetic Materials
大腸菌の取扱い
- 大腸菌コンピテントセル
- 大腸菌組換え蛋白質の調製
- スタブ寒天からの菌の培養
白金耳でスタブ寒天の菌の生えているところに突き刺して菌をとり、寒天プレートにストリークし、培養します。
プラスミド、コスミド、BAC クローン
ゲノムDNA
哺乳動物培養細胞
組換えウイルス
Technical notes
Sequencing & PCR primer
- Sequencing & PCR primers
- Primers for PCR of rRNA gene (Microbe Genomic DNA Information)
- List of STS Markers for Detection of Virus Genes
寄託された遺伝子材料を用いたプロトコール
- 組換えガレクチンタンパク質の発現と精製
Resource page Protocol Lectin (GSB0048) – Gene Set Collection 西 望「組換えガレクチンタンパク質の発現と精製に関するノート」 Glycoforum Vol.23 (5), A15 (2020). DOI: https://doi.org/10.32285/glycoforum.23A15J. - アカネズミ（Apodemus speciosus）染色体特異的 DNA プローブを用いた FISH による染色体ペインティング法 (久保田 善久博士) [pdf]
Kawagoshi, T., Chromosomal Aberrations in Large Japanese Field Mice (Apodemus speciosus) Captured near Fukushima Dai-ichi Nuclear Power Plant. Environ. Sci. Technol. 51 (8): 4632-4641 (2017). PMID 28363014.Catalog # Name of clone Running title RDB16699 Painting probe DNA for A. speciosus chromosome 1 Painting probe DNA for A. speciosus chromosome 1. RDB16700 Painting probe DNA for A. speciosus chromosome 2 Painting probe DNA for A. speciosus chromosome 2. RDB16701 Painting probe DNA for A. speciosus chromosome 3 and 4 Painting probe DNA for A. speciosus chromosome 3 and 4. RDB16702 Painting probe DNA for A. speciosus chromosome 5 Painting probe DNA for A. speciosus chromosome 5. RDB16703 Painting probe DNA for A. speciosus chromosome 6 Painting probe DNA for A. speciosus chromosome 6. RDB16704 Painting probe DNA for A. speciosus chromosome 7 Painting probe DNA for A. speciosus chromosome 7. RDB16705 Painting probe DNA for A. speciosus chromosome 8 Painting probe DNA for A. speciosus chromosome 8. RDB16706 Painting probe DNA for A. speciosus X chromosome Painting probe DNA for A. speciosus X chromosome. - Purification of Recombinant Tubulin by Dr. Etsuko Muto [pdf]
Catalog # Resource name Description RDB12753 pFBD-Hs alpha1CGH/beta3CGF Plasmid clone for baculoviral expression of alpha1 and beta3 tubulin. RDB12754 pFBD-Hs alpha1(K40R)CGH/beta3CGF Plasmid clone for baculoviral expression of alpha1 and beta3 tubulin. RDB14530 pFBD-Hs alpha1 CFXaGH/beta3 CFXaGF Expression vector of His tagged human tubulin alpha-1and Flag tagged human tubulin beta-3. RDB14531 pFBD-Hs alpha1(K40R) CFXaGH/beta3 CFXaGF Expression vector of His tagged human tubulin alpha-1(mutant, K40R, constitutive active form) and Flag tagged human tubulin beta-3. - Expression of heterologous proteins in Drosophila Schneider S2 (S2)
Resource page Protocol Empty Backbone for Insect Cell Experiment A single plasmid transfection that offers a significant advantage associated with puromycin selection in Drosophila Schneider S2 cells expressing heterologous proteins.
Iwaki, T., Castellino, F.J.
Cytotechnology 57 (1), 45-49, (2008). PubMed PMID 19003171. - Fluorescence in situ hybridization using bacterial artificial chromosome (BAC) clones
Resource page Protocol Chinese hamster genomic BAC library clone Fluorescence in situ hybridization using bacterial artificial chromosome (BAC) clones for the analysis of chromosome rearrangement in Chinese hamster ovary cells.
Cao, Y., Kimura, S., Itoi, T., Honda, K., Ohtake, H., Omasa, T.
Methods 56 (3): 418-423 (2012). PubMed PMID 22100493. - Visualization of sphingolipids
Resource page Protocol Genes involved in the sphingolipid signaling pathway Dynamics of sphingomyelin-and cholesterol-enriched lipid domains during cytokinesis.
Abe, M., Kobayashi, T.
Methods Cell Biol. 137: 15-24 (2017). PubMed PMID 28065303. - Optogenetic technology using cultured cell lines
Resource page Protocol Genetic Resources for Optgenetics Research Myogenic Maturation by Optical-Training in Cultured Skeletal Muscle Cells.
Asano, T., Ishizuka, T., Yawo, H.
Methods Mol. Biol. 1668: 135-145 (2017). PubMed PMID 28842907. - Cell-free protein synthesis with noncanonical amino acids using Escherichia coli cell extract
Resource page Protocol E. coli strain for producing recombinant proteins incorporating synthetic amino acids Cell-Free Protein Synthesis for Multiple Site-Specific Incorporation of Noncanonical Amino Acids Using Cell Extracts from RF-1 Deletion E. coli Strains.
Seki, E., Yanagisawa, T., Yokoyama, S.
Methods Mol. Biol. 1728: 49-65 (2018). PubMed PMID 29404990. - Cell cycle observation by using Fluorescence Ubiquitination Cell Cycle Indicator (Fucci)
Resource page Protocol Fucci – Fluorescent Ubiquitination-based Cell Cycle Indicator Methods to study the proliferation and differentiation of cardiac side population (CSP) cells.
Sereti, K.I., Oikonomopoulos, A., Unno, K., Liao, R.
Methods Mol. Biol. 1036: 95-106 (2013). PubMed PMID 23807790. - Transduction of gene to fission yeast by pDUAL and pDUAL2 vectors
Resource page Protocol Expression Vector Backbone of S. pombe Heterologous gene expression by chromosomal integration in fission yeast.
Matsuyama, A., Yoshida, M.
Methods Mol. Biol. 824: 433-450 (2012). PubMed PMID 22160913. - E. coli-based in-cell NMR experiment
Resource page Protocol Thermus thermophilus Expression plasmid In-cell NMR of intrinsically disordered proteins in prokaryotic cells.
Ito, Y., Mikawa, T., Smith, B.O.
Methods Mol. Biol., 895: 19-31 (2012). PubMed PMID 22760309.
Reference of Genetic Materials
- List of references of genetic materials (promoters, plasmid vectors and so on).
- 利用者による学術論文の成果
遺伝子材料開発室より入手したＤＮＡやウイルス株を用いて成果を論文発表される場合、必ず理研BRCならびに文部科学省ナショナルバイオリソースプロジェクトへの謝辞 (Acknowledgement) をAcknowledgement欄やMaterials & Methods欄に記載して頂くようお願い申し上げます。 - プロトコル、マニュアル (理研BRC ウェブサイト) [link]
(MAN0000j 2016.06.21 T.M.)
2021.03.29
