遺伝子材料開発室 – DNA BANK –

Fucci – Fluorescent Ubiquitination-based Cell Cycle Indicator

Fucci5

Ando, R., et al. Cell Struct. Funct. 48 (2): 135-144 (2023). PMID 37394513.

DNA materials

Cat. no.	Resource name	Description
RDB18220	tFucci(SA)5/pCS2	CMV promoter, hCdt1, AzaleaB5, hGeminin, h2-3
RDB18221	tFucci(CA)5/pCS2	CMV promoter, hCdt1, AzaleaB5, hGeminin, h2-3

Cell line

Cat. no.	Resource name
RCB4917	HeLa/Fucci(SA)5#16
RCB4919	HeLa/Fucci(CA)5#16

Cell cycle indicator Fucci(CA) and Fucci(SA)

Sakaue-Sawano, A. et al., Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

DNA materials

Cat. no.	Resource name	Description
RDB15459	mCherry-hCdt1(1/100)Cy(-)/pcDNA3	CMV promoter, hCdt1, mCherry
RDB15273	mCherry-hCdt1(30/120) / pCSII-EF	EF-1α promoter, hCtd1, mCherry, lentivirus vector
RDB15441	mCherry-hCdt1(1/100)Cy(-)/pCSII-EF	EF-1α promoter, hCtd1, mCherry, lentivirus vector
RDB15442	mCherry-hCdt1(1/100)/pCSII-EF	EF-1α promoter, hCtd1, mCherry, lentivirus vector
RDB15271	mVenus-hGeminin(1/110) / pCSII-EF	EF-1α promoter, hGeminin, mVenus, lentivirus vector
RDB15446	tFucci(CA)2/pCSII-EF	EF-1α promoter, hCtd1, mCherry, hGeminin, mVenus, lentivirus vector
RDB15452	tFucci(CA)2/pCSII-CMV	CMV promoter, hCtd1, mCherry, hGeminin, mVenus, lentivirus vector
RDB15447	tFucci(CA)2.1/pCSII-EF	EF-1α promoter, hCtd1, mCherry, hGeminin, AmCyan, lentivirus vector
RDB15453	tFucci(CA)2.1/pCSII-CMV	CMV promoter, hCtd1, mCherry, hGeminin, AmCyan, lentivirus vector
RDB15448	tFucci(CA)2.2/pCSII-EF	EF-1α promoter, hCtd1, mCherry, hGeminin, mTurquoise, lentivirus vector
RDB15454	tFucci(CA)2.2/pCSII-CMV	CMV promoter, hCtd1, mCherry, hGeminin, mTurquoise, lentivirus vector
RDB15443	tFucci(SA)2/pCSII-EF	EF-1α promoter, hCtd1, mCherry, hGeminin, mVenus, lentivirus vector
RDB15449	tFucci(SA)2/pCSII-CMV	CMV promoter, hCtd1, mCherry, hGeminin, mVenus, lentivirus vector
RDB15444	tFucci(SA)2.1/pCSII-EF	EF-1α promoter, hCtd1, mCherry, hGeminin, AmCyan, lentivirus vector
RDB15450	tFucci(SA)2.1/pCSII-CMV	CMV promoter, hCtd1, mCherry, hGeminin, AmCyan, lentivirus vector
RDB15445	tFucci(SA)2.2/pCSII-EF	EF-1α promoter, hCtd1, mCherry, hGeminin, mTurquoise, lentivirus vector
RDB15451	tFucci(SA)2.2/pCSII-CMV	CMV promoter, hCtd1, mCherry, hGeminin, mTurquoise, lentivirus vector

Cell line

Cat. no.	Resource name
RCB4676	HeLa/Fucci(CA)2
RCB4675	NMuMG/Fucci(CA)2.1

Cell cycle indicator Fucci2a

Ford, M.J. et al., Dev. Cell 47 (4): 509-523.e5 (2018). PMID 30458140.
Mort, R.L. et al., Cell Cycle 13 (17): 2681-2696 (2014). PMID 25486356.

DNA materials

Cat. no.	Resource name	Description
RDB16057	pCDNA5-Frt-CAG-Arl13bCerulean-Fucci2a	CAG promoter, Arl13b, Cerulean, hGem(1/110), mVenus, hCdt1(30/120), mCherry
RDB16058	pRosa26-CAG-floxNeo-Arl13bCerulean-Fucci2a	>CAG promoter, Arl13b, Cerulean, hGem(1/110), mVenus, hCdt1(30/120), mCherry
RDB16059	pCAG-Arl13bCerulean-Fucci2a	>CAG promoter, Arl13b, Cerulean, hGem(1/110), mVenus, hCdt1(30/120), mCherry
RDB13080	pCAG-Fucci2a	CAG promoter, hGem(1/110), mVenus, hCdt1(30/120), mCherry
RDB13081	pROSA-floxNeo-Fucci2a	CAG promoter, hGem(1/110), mVenus, hCdt1(30/120), mCherry
RDB16056	pCDNA5-Frt-Fucci2a	CMV promoter, hGem(1/110), mVenus, hCdt1(30/120), mCherry

Mouse

Cat. no.	Resource name
RBRC 06511	B6;129-Gt(ROSA)26Sor<tm1(Fucci2aR)Jkn>

Cell line

Cat. no.	Resource name
AES0223	MESC Fucci2aR
AES0224	MESC Fucci2a
RCB5029	NIH3T3 Flp Arl13bCerulean-Fucci2a
RCB5030	NIH3T3 Flp Fucci2a
AES0225	MESC Arl13b-Fucci2aR
AES0226	MESC Arl13b-Fucci2a

Cell cycle indicator Fucci2

Sakaue-Sawano, A. et al., BMC Cell Biol. 12: 2 (2011). PMID 21226962.
Sakaue-Sawano, A. et al., Chem. Biol. 15 (12): 1243-1248 (2008). PMID 9101468.

DNA materials

Cat. no.	Resource name	Description
RDB15457	mCherry-hCdt1(30/120)/pcDNA3	CMV promoter, hCdt1, mCherry
RDB15458	mVenus-hGeminin(1/110)/pcDNA3	CMV promoter, hGeminin, mVenus
RDB15273	mCherry-hCdt1(30/120) / pCSII-EF	EF-1α promoter, hCtd1, mCherry, lentivirus vector
RDB15271	mVenus-hGeminin(1/110) / pCSII-EF	EF-1α promoter, hGeminin, mVenus, lentivirus vector
RDB15274	AmCyan-hGem(1/110) / pCSII-EF	EF-1α promoter, hGeminin, AmCyan, lentivirus vector

Cell line

Cat. no.	Resource name
RCB2867	HeLa/Fucci2
RCB2868	NMuMG/Fucci2

Cell cycle indicator Fucci

Sakaue-Sawano, A. et al., Development 140 (22): 4624-4632 (2013). PMID 24154524.
Aiba, Y, et al., Proc. Natl. Acad. Sci. USA. 107 (27): 12192-12197 (2010). PMID 20547847.
Sakaue-Sawano, A., Cell 132 (3): 487-498 (2008). PMID 18267078.

DNA materials

Cat. no.	Resource name	Description
RDB15267	mKO2-hCdt1(30/120) / pCSII-EF	EF-1α promoter, hCtd1, mKO2, lentivirus vector
RDB15268	mAG-hGeminin(1/110) / pCSII-EF	EF-1α promoter, hGeminin, mAG, lentivirus vector

Mouse

Cat. no.	Resource name
RBRC02706	B6.Cg-Tg (FucciS/G2/M) #504Bsi
RBRC02707	B6.Cg-Tg (FucciG1) #596Bsi
RBRC02704	B6.Cg-Tg(FucciS/G2/M)#474Bsi
RBRC02708	B6.Cg-Tg(FucciG1)#610Bsi
RBRC02705	B6.Cg-Tg(FucciS/G2/M)#492Bsi
RBRC02709	B6.Cg-Tg(FucciG1)#639Bsi

Cell line

Cat. no.	Resource name
RCB2812	HeLa/Fucci
RCB2813	NMuMG/Fucci
RCB2814	COS/Fucci

Cell cycle indicator

Sakaue-Sawano, A. et al., Chem. Biol. 15 (12): 1243-1248 (2008). PMID 19101468.

DNA materials

Cat. no.	Resource name	Description
RDB15269	mCherry-hGeminin(1/110) / pCSII-EF	EF-1α promoter, hGeminin, mCherry, lentivirus vector
RDB15270	mCherry-hGeminin(1/60) / pCSII-EF	EF-1α promoter, hGeminin, mCherry, lentivirus vector
RDB15272	mVenus-hGeminin(1/60) / pCSII-EF	EF-1α promoter, hGeminin, mVenus, lentivirus vector
RDB15274	AmCyan-hGem(1/110)/pCSII-EF	EF-1α promoter, hGeminin, AmCyan, lentivirus vector

Tol2 plasmid

Sugiyama, M. et al., Proc. Natl. Acad. Sci. USA. 106 (49): 20812-20817 (2009). PMID 19923430.

DNA materials

Cat. no.	Resource name
RDB15278	mCherry-zCdt1(1/190) / pT2KXIGdeltain
RDB15279	mVenus-zGeminin (1/100) / pT2KXIGdeltain
RDB15275	mKO2-zCdt1(1/190) / pT2KXIGdeltain
RDB15276	mAG-zGeminin(1/100) / pT2KXIGdeltain
RDB15277	mAG-hGeminin (1/60) / pT2KXIGdeltain

Other Fucci related materials

RIKEN Center for Brain Science (CBS), Dr. Miyawaki lab.

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References

Important notice regarding nomenclature of Fucci

• 宮脇先生からのお知らせです。[link]ご一読いただきますようお願いいたします。

Mouse of the Month

• March 2009: Fucci mice for visualizing cell-cycle in vivo [link]

プレス発表

• 細胞周期の間期（G1・S・G2）を3色で識別する技術の開発 (AMED, 2017) [link]
• 細胞周期の間期（G1・S・G2）を3色で識別する技術の開発 (RIKEN, 2017) [link]
• ヒト細胞におけるミトコンドリアDNAの維持機構を発見 (名古屋大学, 2017) [link]
• 蛍光イメージング技術によって抗がん剤の作用を再評価 (RIKEN, 2011) [link]
• DNA 複製や細胞分裂の様子をリアルタイムで観察する新技術 (JST, 2008) [link]
• DNA 複製や細胞分裂の様子をリアルタイムで観察する新技術 (RIKEN, 2008) [pdf]

(GRP0044j 2017.09.18 T.M.)

2024.09.13