概要
HLA抗原は、臓器移植ならびに輸血の際の拒絶反応に関係する蛋白質です。
特に、日本人に頻度の高いHLAクラスI抗原をコードするcDNAクローンを愛知県がんセンター研究所の赤塚美樹博士より寄託して頂きました。赤塚先生には新しいクローンが同定され次第、寄託して頂いております。各HLAクローンについてのさらに詳しい情報は、HLA Clone Listをご覧下さい。
関連情報
HLA抗原についての詳しい情報は日本組織適合性学会ホームページをご参照下さい。
HLA情報も付属した日本人由来不死化細胞株 (EBV transformed B cell : HEV) は、細胞材料開発室から提供しています。
提供形態
HLAクローンは、プラスミドDNAを抽出し、DNAの制限酵素切断パターンの電気泳動ならびに挿入遺伝子の末端塩基配列の検定の後、DNA溶液(1 μg) として利用者に室温で送付しています。できるだけ早くご使用下さい。
書類の入手と記入
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書式A （遺伝子材料提供依頼書） [Word] 1部 - 書式C
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書式C (生物遺伝資源提供同意書, MTA) 非営利学術目的用 [Word]
書式C (生物遺伝資源提供同意書, MTA) 営利目的用 [Word]2部 - その他の書式
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〒305-0074 茨城県つくば市高野台3-1-1
理化学研究所バイオリソース研究センター
遺伝子材料開発室
E-mail: dnabank.brc@riken.jp
TEL: 029-836-3612 (平日の9:00～12:00 / 13:00～17:00)
FAX: 029-836-9120 - 提供申込の前に、「申込書類の記入と送付」「ご利用にあたって」「遺伝子材料の品質管理」をご覧ください。
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提供手数料：（1本あたり)
非営利学術目的 8,440円
営利目的 16,880円
遺伝子材料提供の概要はこちら。
書類の送付先、問い合わせ先
〒305-0074 茨城県つくば市高野台3-1-1
理化学研究所バイオリソース研究センター
遺伝子材料開発室
E-mail: dnabank.brc@riken.jp
TEL: 029-836-3612 (平日の9:00～12:00 / 13:00～17:00)
FAX: 029-836-9120
HLA Clone List
HLA Class I
| Antigen | Allele | Cat. No. | Name of Clone | Frequency in Japanese population | |||
|---|---|---|---|---|---|---|---|
| freq.1 (%) | freq.2 (%) | freq.3 (%) | freq.4 (%) | ||||
| HLA-A1 | A*01:01 | RDB03368 | Human HLA-A*01:01 cDNA | 1.8 | 0.2 | 0.57 | 0.25 |
| HLA-A2 | A*02:06 | RDB02867 | Human HLA-A*02:06 cDNA | 8.4 | 10.4 | 8.99 | 8.74 |
| HLA-A2 | A*02:07 | RDB02868 | Human HLA-A*02:07 cDNA | 4.0 | 3.4 | 2.87 | 3.39 |
| HLA-A2 | A*02:01:01 | RDB02866 | Human HLA-A*02:01:01 cDNA | 10.6 | 10.9 | 10.71 | 11.64 |
| HLA-A3 | A*03:01 | RDB03367 | Human HLA-A*03:01 cDNA | r | 0.8 | 0.57 | 0.25 |
| HLA-A11 | A*11:01:01 | RDB02869 | Human HLA-A*11:01:01 cDNA | 11.1 | 8.1 | 10.71 | 10.22 |
| HLA-A24 | RDB01176 | pA01 | |||||
| HLA-A24 | A*24:02:01 | RDB02871 | Human HLA-A*24:02:01 cDNA | 32.7 | 35.6 | 36.52 | 36.20 |
| HLA-A24 | A*24:20 | RDB03365 | Human HLA-A*24:20 cDNA | vr | vr | 0.83 | |
| HLA-A26 | A*26:01 | RDB02870 | Human HLA-A*26:01 cDNA | 6.2 | 9.8 | 11.28 | 7.66 |
| HLA-A26 | A*26:03 | RDB03366 | Human HLA-A*26:03 cDNA | 1.3 | 2.1 | 1.91 | 1.47 |
| HLA-A31 | A*31:01:02 | RDB02872 | Human HLA-A*31:01:02 cDNA | 9.3 | 7.7 | 6.88 | 9.14 |
| HLA-A33 | A*33:03 | RDB02873 | Human HLA-A*33:03 cDNA | 12.8 | 7.9 | 7.84 | 6.68 |
| 98.2 | 96.9 | 98.85 | 96.47 | ||||
| Antigen | Allele | Cat. No. | Name of Clone | Frequency in Japanese population | |||
|---|---|---|---|---|---|---|---|
| freq.1 (%) | freq.2 (%) | freq.3 (%) | freq.4 (%) | ||||
| HLA-B7 | B*07:02:01 | RDB02874 | Human HLA-B*07:02:01 cDNA | 5.2 | 5.2 | 3.63 | 5.70 |
| HLA-B8 | B*08:01 | RDB03369 | Human HLA-B*08:01 cDNA | ||||
| HLA-B15 | B*15:01:01 | RDB02875 | Human HLA-B*15:01:01 cDNA | 6.5 | 7.2 | 9.37 | 7.51 |
| HLA-B35 | B*35:01:01 | RDB02876 | Human HLA-B*35:01:01 cDNA | 7.8 | 8.6 | 8.80 | 7.76 |
| HLA-B38 | B*38:01 | RDB03370 | Human HLA-B*38:01 cDNA | ||||
| HLA-B39 | B*39:01:01 | RDB03371 | Human HLA-B*39:01:01 cDNA | 3.5 | r | 4.02 | 3.44 |
| HLA-B40 | B*40:01:02 | RDB02877 | Human HLA-B*40:01:02 cDNA | 6.1 | 5.1 | 5.35 | 5.40 |
| HLA-B40 | B*40:02 | RDB02878 | Human HLA-B*40:02 cDNA | 6.1 | 8.2 | 8.41 | 8.25 |
| HLA-B44 | B*44:01 | RDB01178 | AM2.60 | ||||
| HLA-B44 | B*44:03:01 | RDB02879 | Human HLA-B*44:03:01 cDNA | 12.2 | 6.9 | 6.69 | 6.24 |
| HLA-B46 | B*46:01 | RDB02880 | Human HLA-B*46:01 cDNA | 6.1 | 3.8 | 3.44 | 4.96 |
| HLA-B48 | B*48:01 | RDB03372 | Human HLA-B*48:01 cDNA | 4.3 | 2.7 | 5.54 | 2.65 |
| HLA-B51 | B*51:01:01 | RDB02881 | Human HLA-B*51:01:01 cDNA | 7.0 | 7.9 | 6.88 | 8.35 |
| HLA-B52 | B*52:01:01 | RDB02882 | Human HLA-B*52:01:01 cDNA | 10.4 | 13.7 | 0.19 | 10.90 |
| HLA-B54 | B*54:01 | RDB03373 | Human HLA-B*54:01 cDNA | 7.0 | 6.5 | 7.07 | 8.79 |
| HLA-B55 | B*55:02 | RDB03374 | Human HLA-B*55:02 cDNA | 2.2 | 3.2 | 2.87 | 1.92 |
| HLA-B55 | RDB01180 | pB55 | |||||
| HLA-B59 | B*59:01 | RDB03375 | Human HLA-B*59:01 cDNA | 0.9 | 1.7 | 1.72 | 1.96 |
| 85.3 | 80.7 | 73.98 | 83.83 | ||||
| Antigen | Allele | Cat. No. | Name of Clone | Frequency in Japanese population | |||
|---|---|---|---|---|---|---|---|
| freq.1 (%) | freq.2 (%) | freq.3 (%) | freq.4 (%) | ||||
| HLA-Cw1 | C*01:02 | RDB02883 | Human HLA-C*01:02 cDNA | 17.0 | 16.63 | ||
| HLA-Cw1 | C*01:03 | RDB03376 | Human HLA-C*01:03 cDNA | r | |||
| HLA-Cw3 | C*03:03:01 | RDB02884 | Human HLA-C*03:03:01 cDNA | 7.8 | 13.19 | ||
| HLA-Cw3 | C*03:04:01 | RDB02885 | Human HLA-C*03:04:01 cDNA | 11.3 | 13.77 | ||
| HLA-Cw4 | C*04:01:01 | RDB02886 | Human HLA-C*04:01:01 cDNA | 6.5 | 4.40 | ||
| HLA-Cw6 | C*06:02 | RDB03377 | Human HLA-C*06:02 cDNA | 1.7 | 1.34 | ||
| HLA-Cw7 | C*07:02 | RDB02887 | Human HLA-C*07:02 cDNA | 11.3 | 10.33 | ||
| HLA-Cw8 | C*08:01 | RDB02888 | Human HLA-C*08:01 cDNA | 10.9 | 9.75 | ||
| HLA-Cw12 | C*12:02:02 | RDB02889 | Human HLA-C*12:02:02 cDNA | 10.4 | 11.09 | ||
| HLA-Cw14 | C*14:02:01 | RDB03378 | Human HLA-C*14:02:01 cDNA | 5.7 | 4.78 | ||
| HLA-Cw14 | C*14:03 | RDB02890 | Human HLA-C*14:03 cDNA | 12.2 | 7.07 | ||
| HLA-Cw15 | C*15:02:01 | RDB03379 | Human HLA-C*15:02:01 cDNA | 1.7 | 4.40 | ||
| HLA-Cx 52 (Cw 12) | C*12:01 | RDB01177 | pAC201 | ||||
| HLA-Cx52 (Cw12) | C*12:01 | RDB01179 | pC68 | ||||
| 96.5 | 96.75 | ||||||
| HLA-G | RDB01246 | HLA-G.7.0 E | |||||
Freq.1: frequency in Japanese population (N=117), Tokunaga K. et al. Immunogenetics 1997; 46(3)199-205.
Freq.2: frequency in Japanese population (N=493), Tanaka H. et al. Clinical Transplants 1996; 139-144.
Freq.3: frequency in Japanese population (N=523), Nakajima, et al. MHC Vol.8 No.1 p1-32.
Freq.4: frequency in Japanese population (N=1018), Azuma F., et al., MHC, p166, 2004.
r: rare; vr: very rare.
| Gene Symbol | Product Name | Synonyms |
|---|---|---|
| HLA-A | major histocompatibility complex, class I, A | HLAA |
| HLA-B | major histocompatibility complex, class I, B | AS|B-4901|HLAB |
| HLA-C | major histocompatibility complex, class I, C | D6S204|HLA-JY3|HLAC|HLC-C|MHC|PSORS1 |
| HLA-E | major histocompatibility complex, class I, E | HLA-6.2|QA1 |
| HLA-F | major histocompatibility complex, class I, F | CDA12|HLA-5.4|HLA-CDA12|HLAF |
| HLA-G | major histocompatibility complex, class I, G | MHC-G |
HLA Class II
| Antigen | Allele | Cat. No. | Name of Clone |
|---|---|---|---|
| HLA DR beta 5 | RDB01222 | TOK H5 DR beta | |
| HLA-DR beta 4 | RDB01223 | YT158 | |
| HLA-DQA1 | RDB01227 | pgDQ4A | |
| HLA-DQB1 | RDB01228 | pg DQ1B | |
| HLA-DQB1 | RDB01230 | pg DQ1BS | |
| HLA-DRA | RDB01232 | DRA2EH | |
| HLA-DPA1 | RDB01233 | DPA 02022 | |
| HLA-DPB1 | RDB01234 | DPB0202 | |
| HLA-DRB1 | RDB01235 | K^b DRB1 0803 | |
| HLA-DRB1 | RDB01236 | K^b DRB1 1201 | |
| HLA-DRB1 | RDB01237 | K^b DRB1 1302 | |
| HLA-DRB3 | RDB01238 | DRB30301 EMJ-4 | |
| HLA-DQA1 | RDB01239 | DQA10501 AMALA-4 | |
| HLA-DQB1 | RDB01240 | DQB10301 AMALA-4 | |
| HLA-DQA1 | RDB01241 | DQA10101 KAS116 3-6 | |
| HLA-DQB1 | RDB01242 | DQB10503 EK2-4 |
| Gene Symbol | Product Name | Synonyms |
|---|---|---|
| HLA-DMA | major histocompatibility complex, class II, DM alpha | D6S222E|DMA|HLADM|RING6 |
| HLA-DMB | major histocompatibility complex, class II, DM beta | D6S221E|RING7 |
| HLA-DOA | major histocompatibility complex, class II, DO alpha | HLA-DNA|HLA-DZA|HLADZ |
| HLA-DOB | major histocompatibility complex, class II, DO beta | DOB|HLA_DOB |
| HLA-DPA1 | major histocompatibility complex, class II, DP alpha 1 | DP(W3)|DP(W4)|HLA-DP1A|HLADP|HLASB|PLT1 |
| HLA-DPB1 | major histocompatibility complex, class II, DP beta 1 | DPB1|HLA-DP|HLA-DP1B|HLA-DPB |
| HLA-DPB2 | major histocompatibility complex, class II, DP beta 2 (pseudogene) | DP2B|DPB2|DPbeta2|HLA-DP2B |
| HLA-DQA1 | major histocompatibility complex, class II, DQ alpha 1 | CELIAC1|DQ-A1|HLA-DQA |
| HLA-DQB1 | major histocompatibility complex, class II, DQ beta 1 | CELIAC1|HLA-DQB|IDDM1 |
| HLA-DQB2 | major histocompatibility complex, class II, DQ beta 2 | HLA-DQB1|HLA-DXB |
| HLA-DRA | major histocompatibility complex, class II, DR alpha | HLA-DRA1 |
| HLA-DRB1 | major histocompatibility complex, class II, DR beta 1 | DRB1|HLA-DR1B|HLA-DRB|SS1 |
| HLA-DRB3 | major histocompatibility complex, class II, DR beta 3 | HLA-DR1B|HLA-DR3B |
| HLA-DRB4 | major histocompatibility complex, class II, DR beta 4 | DR4|DRB4|HLA-DR4B |
| HLA-DRB5 | major histocompatibility complex, class II, DR beta 5 | – |
References for HLA
- Akatsuka Y., Morishima Y., Kuzushima K., Kodera Y., Takahashi T. (2007) Clinical relevance of a newly identified HLA-A24-restricted minor histocompatibility antigen epitope derived from BCL2A1, ACC-1, in patients receiving HLA genotypically matched unrelated bone marrow transplant.
Cancer Science 98 (8): 1139-1146. - Nishida T, Akatsuka Y, Morishima Y, Hamajima N, Tsujimura K, Kuzushima K, Kodera Y, Takahashi T. (2004) Clinical relevance of a newly identified HLA-A24-restricted minor histocompatibility antigen epitope derived from BCL2A1, ACC-1, in patients receiving HLA genotypically matched unrelated bone marrow transplant.
Br. J. Haematol. 124 (5): 629-635. - Akatsuka Y, Nishida T, Kondo E, Miyazaki M, Taji H, Iida H, Tsujimura K, Yazaki M, Naoe T, Morishima Y, Kodera Y, Kuzushima K, Takahashi T. (2003) Identification of a Polymorphic Gene, BCL2A1, Encoding Two Novel Hematopoietic Lineage-specific Minor Histocompatibility Antigens.J. Exp. Med. 197 (11): 1489-1500.
- Akatsuka Y, Goldberg TA, Kondo E, Martin EG, Obata Y, Morishima Y, Takahashi T, Hansen JA (2002) Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines.
Tissue Antigens, 59 (6): 502-511. - Akatsuka Y, Kondo E, Taji H, Morishima Y, Yazaki M, Obata Y, Kodera Y, Riddell SR, Takahashi T. (2002) Targeted cloning of cytotoxic T cells specific for minor histocompatibility antigens restricted by HLA class I molecules of interest.
Transplantation, 74: 1773-1780. - Ando H, Miyata Y, Takata H, Inoko H. (1993) Identification of alloantisera reacting with HLA-C blank (Cx52) using a mouse L-cell transfected with the HLA-Cw*1201 allele.
Hum Immunol. 36 (2): 76-80 - Senju S, Kimura A, Yasunami M, Kamikawaji N, Yoshizumi H, Nishimura Y, Sasazuki T. (1992) Allele-specific expression of the cytoplasmic exon of HLA-DQB1 gene.
Immunogenetics 36 (5): 319-325. - Zemmour J, Parham P. (1991) HLA class I nucleotide sequences, 1991.
Tissue Antigens 37 (4): 174-180. Erratum in: Tissue Antigens. 1992 Nov;40(5):221-228. - Fujisawa K, Kamikawaji N, Yasunami M, Kimura A, Nishimura Y, Sasazuki T. (1991) High precursor frequency of human T cells reactive to HLA-DQ molecules expressed on mouse L cell transfectants.
Eu.r J Immunol. 21 (10): 2341-2347. - Kamikawaji N, Fujisawa K, Yoshizumi H, Fukunaga M, Yasunami M, Kimura A, Nishimura Y, Sasazuki T. (1991) HLA-DQ-restricted CD4+ T cells specific to streptococcal antigen present in low but not in high responders.
J Immunol. 146 (8): 2560-2567. - Tamaki J. (1992)[Analysis of polymorphism at the human class I MHC locus, HLA-G]
Hokkaido Igaku Zasshi (Hokkaido J. Med. Sci.) 67 (5): 583-594. Japanese. - Nishimura Y, Iwanaga T, Inamitsu T, Yanagawa Y, Yasunami M, Kimura A, Hirokawa K, Sasazuki T. (1990) Expression of the human MHC, HLA-DQW6 genes alters the immune response in C57BL/6 mice.
J Immunol. 1990 Jul 1;145(1):353-60. - Takata H, Inoko H, Ando A, Haranaka M, Watanabe B, Tsuji K, Iri H. (1988) Cloning and analysis of HLA class I cDNA encoding a new HLA-C specificity Cx52.
Immunogenetics 28 (4): 265-270.
Related Information
(GSB0016j 2004.08.23 T.M.)
2018.11.15