概要
HLA抗原は、臓器移植ならびに輸血の際の拒絶反応に関係する蛋白質です。
特に、日本人に頻度の高いHLAクラスI抗原をコードするcDNAクローンを愛知県がんセンター研究所の赤塚美樹博士より寄託して頂きました。他のクラスのヒトHLAやbeta-2 microglobulin (β2m)のcDNAクローンも利用可能です。
HLA Clone List
HLA Class I – cDNA clone
| Allele | Cat. no. | Name of Clone | Frequency in Japanese population (*) |
|---|---|---|---|
| A*01:01 | RDB03368 | Human HLA-A*01:01 cDNA | 0.439 |
| A*01:01 | HGY082896 | IRAL007D24 | |
| A*02:01 | RDB02866 | Human HLA-A*02:01:01 cDNA | 11.223 |
| A*02:06 | RDB02867 | Human HLA-A*02:06 cDNA | 9.432 |
| A*02:07 | RDB02868 | Human HLA-A*02:07 cDNA | 3.241 |
| A*03:01 | RDB03367 | Human HLA-A*03:01 cDNA | 0.434 |
| A*11:01 | RDB02869 | Human HLA-A*11:01:01 cDNA | 8.9 |
| A*24:02 | RDB02871 | Human HLA-A*24:02:01 cDNA | 36.266 |
| A*24:20 | RDB03365 | Human HLA-A*24:20 cDNA | 0.768 |
| A*25:01 | HGX004917 | IRAK012E21 | 0.001 |
| A*26:01 | RDB02870 | Human HLA-A*26:01 cDNA | 7.592 |
| A*26:03 | RDB03366 | Human HLA-A*26:03 cDNA | 2.509 |
| A*31:01 | RDB02872 | Human HLA-A*31:01:02 cDNA | 8.642 |
| A*33:03 | RDB02873 | Human HLA-A*33:03 cDNA | 7.399 |
| HGY080967 | IRAL002G23 | ||
| RDB01176 | pA01 |
*Major Histocompatibility Complex 30 (1): 17-19 (2023).
| Allele | Cat. no. | Name of Clone | Frequency in Japanese population (*) |
|---|---|---|---|
| B*07:02 | RDB02874 | Human HLA-B*07:02:01 cDNA | 5.475 |
| B*08:01 | RDB03369 | Human HLA-B*08:01 cDNA | 0.018 |
| B*15:01 | RDB02875 | Human HLA-B*15:01:01 cDNA | 7.92 |
| B*15:10 | HGX010665 | IRAK026L01 | |
| B*35:01 | RDB02876 | Human HLA-B*35:01:01 cDNA | 8.357 |
| B*38:01 | RDB03370 | Human HLA-B*38:01 cDNA | 0.007 |
| B*39:01 | RDB03371 | Human HLA-B*39:01:01 cDNA | 3.387 |
| B*40:01 | RDB02877 | Human HLA-B*40:01:02 cDNA | 5.56 |
| B*40:02 | RDB02878 | Human HLA-B*40:02 cDNA | 7.801 |
| B*44:03 | RDB02879 | Human HLA-B*44:03:01 cDNA | 6.669 |
| B*46:01 | RDB02880 | Human HLA-B*46:01 cDNA | 4.502 |
| B*48:01 | RDB03372 | Human HLA-B*48:01 cDNA | 2.893 |
| B*51:01 | RDB02881 | Human HLA-B*51:01:01 cDNA | 8.743 |
| B*52:01 | RDB02882 | Human HLA-B*52:01:01 cDNA | 11.015 |
| B*54:01 | RDB03373 | Human HLA-B*54:01 cDNA | 7.577 |
| B*55:02 | RDB03374 | Human HLA-B*55:02 cDNA | 2.473 |
| B*59:01 | RDB03375 | Human HLA-B*59:01 cDNA | 2.012 |
| HGY088861 | IRAL022C13 | ||
| RDB01180 | pB55 |
*Major Histocompatibility Complex 30 (1): 17-19 (2023).
| Allele | Cat. no. | Name of Clone | Frequency in Japanese population (*) |
|---|---|---|---|
| C*01:02 | RDB02883 | Human HLA-C*01:02 cDNA | 17.225 |
| C*01:03 | RDB03376 | Human HLA-C*01:03 cDNA | 0.34 |
| C*03:03 | RDB02884 | Human HLA-C*03:03:01 cDNA | 13.753 |
| C*03:04 | RDB02885 | Human HLA-C*03:04:01 cDNA | 12.205 |
| C*04:01 | RDB02886 | Human HLA-C*04:01:01 cDNA | 4.324 |
| C*06:02 | RDB03377 | Human HLA-C*06:02 cDNA | 0.816 |
| C*07:01 | HGX011896 | IRAK029M08 | 0.065 |
| C*07:02 | RDB02887 | Human HLA-C*07:02 cDNA | 12.705 |
| C*07:02 | HGY088291 | IRAL020M03 | |
| C*08:01 | RDB02888 | Human HLA-C*08:01 cDNA | 7.382 |
| C*12:01 | RDB01179 | pC68 | |
| C*12:02 | RDB02889 | Human HLA-C*12:02:02 cDNA | 11.003 |
| C*12:03 | HGX007733 | IRAK019F13 | 0.087 |
| C*14:02 | RDB03378 | Human HLA-C*14:02:01 cDNA | 6.853 |
| C*14:03 | RDB02890 | Human HLA-C*14:03 cDNA | 6.514 |
| C*15:02 | RDB03379 | Human HLA-C*15:02:01 cDNA | 3.054 |
| C*16:01 | HGY082329 | IRAL005N17 | 0.004 |
| C*16:01 | HGY085701 | IRAL014E05 | |
| HGY088506 | IRAL021E10 |
*Major Histocompatibility Complex 30 (1): 17-19 (2023).
| Allele | Cat. no. | Name of Clone |
|---|---|---|
| E*01:01 | HGY081676 | IRAL004D04 |
| E*01:01 | HGY085250 | IRAL013C02 |
| E*01:03 | HGY096623 | IRAL041J07 |
| F*01:01 | HGX054355 | IRAK135O19 |
| F*01:01 | HGY090322 | IRAL025N10 |
| HGY013281 | IRAK033D09 | |
| HGY094732 | IRAL036N20 |
HLA Class I – genomic DNA clone
| Antigen | Allele | Cat. No. | Name of Clone |
|---|---|---|---|
| HLA-B44 | B*44:01 | RDB01178 | AM2.60 |
| HLA-Cx 52 (Cw 12) | C*12:01 | RDB01177 | pAC201 |
| HLA-G | RDB01246 | HLA-G.7.0 E |
HLA Class II
| Allele | Cat. no. | Name of Clone |
|---|---|---|
| DRB1*04:10 | HGY096962 | IRAL042G18 |
| DRB1*07:01 | HGY082333 | IRAL005N21 |
| DRB1*13:02 | HGY088361 | IRAL020P01 |
| DRB1*13:03 | HGY088606 | IRAL021I14 |
| DRB1*15:01 | HGX027583 | IRAK068P23 |
| DRB3*01:01 | HGX005065 | IRAK012L01 |
| DRB4*01:03 | HGY086487 | IRAL016D15 |
| DRB5*01:01 | HGY090353 | IRAL025O17 |
| DRB5*01:02 | RDB01222 | TOK H5 DR beta |
| DQB1*03:02 | HGY091784 | IRAL029H16 |
| DPB1*01:01 | HGX010616 | IRAK026I24 |
| DPB1*17:01 | HGY093632 | IRAL034B08 |
| DPB1*23:01 | HGY088362 | IRAL020P02 |
| DRA*01:02 | HGX025864 | IRAK064K24 |
| DQA1*05:01 | HGX005175 | IRAK012P15 |
| DQA1*05:03 | RDB01239 | DQA10501 AMALA-4 |
| DPA1*02:01 | HGY088364 | IRAL020P04 |
| DMA*01:01 | HGY087766 | IRAL019G22 |
| DMA*01:01 | HGY094834 | IRAL037B10 |
| DMB*01:01 | HGX010628 | IRAK026J12 |
| DOA*01:01 | HGX010612 | IRAK026I20 |
| DOB*01:01 | HGY087568 | IRAL018P08 |
| RDB01235 | K^b DRB1 0803 | |
| RDB01236 | K^b DRB1 1201 | |
| RDB01237 | K^b DRB1 1302 | |
| HGY096628 | IRAL041J12 | |
| RDB01238 | DRB30301 EMJ-4 | |
| RDB01223 | YT158 | |
| RDB01228 | pg DQ1B | |
| RDB01230 | pg DQ1BS | |
| RDB01242 | DQB10503 EK2-4 | |
| RDB01234 | DPB0202 | |
| RDB01232 | DRA2EH | |
| HGX044148 | IRAK110G04 | |
| HGX069737 | IRAK174F17 | |
| RDB01241 | DQA10101 KAS116 3-6 | |
| RDB01233 | DPA 02022 |
Link to HLA gene resources
| Gene Symbol | Product Name | Synonyms |
|---|---|---|
| HLA-A | major histocompatibility complex, class I, A | HLAA |
| HLA-B | major histocompatibility complex, class I, B | AS|B-4901|HLAB |
| HLA-C | major histocompatibility complex, class I, C | D6S204|HLA-JY3|HLAC|HLC-C|MHC|PSORS1 |
| HLA-E | major histocompatibility complex, class I, E | HLA-6.2|QA1 |
| HLA-F | major histocompatibility complex, class I, F | CDA12|HLA-5.4|HLA-CDA12|HLAF |
| HLA-G | major histocompatibility complex, class I, G | MHC-G |
| HLA-DMA | major histocompatibility complex, class II, DM alpha | D6S222E|DMA|HLADM|RING6 |
| HLA-DMB | major histocompatibility complex, class II, DM beta | D6S221E|RING7 |
| HLA-DOA | major histocompatibility complex, class II, DO alpha | HLA-DNA|HLA-DZA|HLADZ |
| HLA-DOB | major histocompatibility complex, class II, DO beta | DOB|HLA_DOB |
| HLA-DPA1 | major histocompatibility complex, class II, DP alpha 1 | DP(W3)|DP(W4)|HLA-DP1A|HLADP|HLASB|PLT1 |
| HLA-DPB1 | major histocompatibility complex, class II, DP beta 1 | DPB1|HLA-DP|HLA-DP1B|HLA-DPB |
| HLA-DPB2 | major histocompatibility complex, class II, DP beta 2 (pseudogene) | DP2B|DPB2|DPbeta2|HLA-DP2B |
| HLA-DQA1 | major histocompatibility complex, class II, DQ alpha 1 | CELIAC1|DQ-A1|HLA-DQA |
| HLA-DQB1 | major histocompatibility complex, class II, DQ beta 1 | CELIAC1|HLA-DQB|IDDM1 |
| HLA-DQB2 | major histocompatibility complex, class II, DQ beta 2 | HLA-DQB1|HLA-DXB |
| HLA-DRA | major histocompatibility complex, class II, DR alpha | HLA-DRA1 |
| HLA-DRB1 | major histocompatibility complex, class II, DR beta 1 | DRB1|HLA-DR1B|HLA-DRB|SS1 |
| HLA-DRB3 | major histocompatibility complex, class II, DR beta 3 | HLA-DR1B|HLA-DR3B |
| HLA-DRB4 | major histocompatibility complex, class II, DR beta 4 | DR4|DRB4|HLA-DR4B |
| HLA-DRB5 | major histocompatibility complex, class II, DR beta 5 | – |
関連情報
HLA抗原についての詳しい情報は日本組織適合性学会ホームページをご参照下さい。
HLA情報も付属した日本人由来不死化細胞株 (EBV transformed B cell : HEV) は、細胞材料開発室から提供しています。
Journals
- Akatsuka Y., Morishima Y., Kuzushima K., Kodera Y., Takahashi T. (2007) Clinical relevance of a newly identified HLA-A24-restricted minor histocompatibility antigen epitope derived from BCL2A1, ACC-1, in patients receiving HLA genotypically matched unrelated bone marrow transplant.
Cancer Science 98 (8): 1139-1146. - Nishida T, Akatsuka Y, Morishima Y, Hamajima N, Tsujimura K, Kuzushima K, Kodera Y, Takahashi T. (2004) Clinical relevance of a newly identified HLA-A24-restricted minor histocompatibility antigen epitope derived from BCL2A1, ACC-1, in patients receiving HLA genotypically matched unrelated bone marrow transplant.
Br. J. Haematol. 124 (5): 629-635. - Akatsuka Y, Nishida T, Kondo E, Miyazaki M, Taji H, Iida H, Tsujimura K, Yazaki M, Naoe T, Morishima Y, Kodera Y, Kuzushima K, Takahashi T. (2003) Identification of a Polymorphic Gene, BCL2A1, Encoding Two Novel Hematopoietic Lineage-specific Minor Histocompatibility Antigens.J. Exp. Med. 197 (11): 1489-1500.
- Akatsuka Y, Goldberg TA, Kondo E, Martin EG, Obata Y, Morishima Y, Takahashi T, Hansen JA (2002) Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines.
Tissue Antigens, 59 (6): 502-511. - Akatsuka Y, Kondo E, Taji H, Morishima Y, Yazaki M, Obata Y, Kodera Y, Riddell SR, Takahashi T. (2002) Targeted cloning of cytotoxic T cells specific for minor histocompatibility antigens restricted by HLA class I molecules of interest.
Transplantation, 74: 1773-1780. - Ando H, Miyata Y, Takata H, Inoko H. (1993) Identification of alloantisera reacting with HLA-C blank (Cx52) using a mouse L-cell transfected with the HLA-Cw*1201 allele.
Hum Immunol. 36 (2): 76-80 - Senju S, Kimura A, Yasunami M, Kamikawaji N, Yoshizumi H, Nishimura Y, Sasazuki T. (1992) Allele-specific expression of the cytoplasmic exon of HLA-DQB1 gene.
Immunogenetics 36 (5): 319-325. - Zemmour J, Parham P. (1991) HLA class I nucleotide sequences, 1991.
Tissue Antigens 37 (4): 174-180. Erratum in: Tissue Antigens. 1992 Nov;40(5):221-228. - Fujisawa K, Kamikawaji N, Yasunami M, Kimura A, Nishimura Y, Sasazuki T. (1991) High precursor frequency of human T cells reactive to HLA-DQ molecules expressed on mouse L cell transfectants.
Eu.r J Immunol. 21 (10): 2341-2347. - Kamikawaji N, Fujisawa K, Yoshizumi H, Fukunaga M, Yasunami M, Kimura A, Nishimura Y, Sasazuki T. (1991) HLA-DQ-restricted CD4+ T cells specific to streptococcal antigen present in low but not in high responders.
J Immunol. 146 (8): 2560-2567. - Tamaki J. (1992)[Analysis of polymorphism at the human class I MHC locus, HLA-G]
Hokkaido Igaku Zasshi (Hokkaido J. Med. Sci.) 67 (5): 583-594. Japanese. - Nishimura Y, Iwanaga T, Inamitsu T, Yanagawa Y, Yasunami M, Kimura A, Hirokawa K, Sasazuki T. (1990) Expression of the human MHC, HLA-DQW6 genes alters the immune response in C57BL/6 mice.
J Immunol. 1990 Jul 1;145(1):353-60. - Takata H, Inoko H, Ando A, Haranaka M, Watanabe B, Tsuji K, Iri H. (1988) Cloning and analysis of HLA class I cDNA encoding a new HLA-C specificity Cx52.
Immunogenetics 28 (4): 265-270.
Links
- Find more MHCI clones by KEGG
- EBV transformed B cell lines derived from Japanese (HEV) with information of HLA haplotypes
- Japanese Society for Histocomaptibility and Immunogenetics
- International Histocompatibility Workshop Group
- HLA Infomatics Group – The Anthony Nolan Trust
(GSB0016j 2004.08.23 T.M.)
2024.07.24
