Fluorescent protein resource
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Ultramarine | Cyan | Green | Yellow | Orange | Red
photoconversion type | photochromism type | Destabilized- and Secreted-FPs | Non-fluorescent chromoprotein
Tools | 耐熱性GFP | pH感受性GFP | Arranged vector
Ultramarine
Sirius
Protein | Resource (cat.num.) | Reference |
---|---|---|
Sirius | Sirius/pcDNA3 (RDB19875) | An ultramarine fluorescent protein with increased photostability and pH insensitivity. Tomosugi, W. et al., Nat. Methods. 6 (5): 351-353 (2009). PMID 19349978. |
External information
Sirius in FPbase
Cyan
Kusabira Cyan
青緑色の蛍光を発します。ECFPに比べて黄色への蛍光スペクトルの重なりが少ないです。
Kusabira-Cyan CoralHue™ Fluorescent Protein | ||||||||
Abbr. | Form | Excitation maxima (nm) | Emission maxima (nm) | S1 vector# | MC1 vector$ | MN1 vector$ | MCLinker vector* | MNLinker vector* |
---|---|---|---|---|---|---|---|---|
KCy1 | Dimer | 453 | 486 | RDB18310 |
Midoriishi Cyan
青緑色の蛍光を発します。ECFPに比べて黄色への蛍光スペクトルの重なりが少ないです。
Midoriishi-Cyan CoralHue™ Fluorescent Protein | ||||||||
Abbr. | Form | Excitation maxima (nm) | Emission maxima (nm) | S1 vector# | MC1 vector$ | MN1 vector$ | MCLinker vector* | MNLinker vector* |
---|---|---|---|---|---|---|---|---|
MiCy1 | Dimer | 472 | 495 | RDB18265 | ||||
mMiCy1 | Monomer | 470 | 496 | RDB18289 | ||||
hmMiCy1 | Monomer | 470 | 496 | RDB18290 | RDB18291 | RDB18292 | RDB18288 |
Protein | Resource (cat.num.) | Reference |
---|---|---|
mMidoriishi-Cyan | mMiCy1/pRSETB (RDB15241) | Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312 (2004). PubMed PMID 15065984. |
External information
Midoriishi Cyan (MiCy) in FPbase
mMidoriishi Cyan (mMiCy) in FPbase
Green
Umikinoko Green
鮮やかな緑色の蛍光を発し、pH安定性を示します。
Umikinoko-Green CoralHue™ Fluorescent Protein | ||||||||
Abbr. | Form | Excitation maxima (nm) | Emission maxima (nm) | S1 vector# | MC1 vector$ | MN1 vector$ | MCLinker vector* | MNLinker vector* |
---|---|---|---|---|---|---|---|---|
mUKG1 | Monomer | 483 | 499 | RDB18306 | ||||
hmUKG1 | Monomer | 483 | 499 | RDB18307 | RDB18308 | RDB18309 |
External information
mUmikinoko-Green (mUkG) in FPbase
Azami Green
緑色の蛍光を発する蛍光タンパク質です。
Azami-Green CoralHue™ Fluorescent Protein | ||||||||
Abbr. | Form | Excitation maxima (nm) | Emission maxima (nm) | S1 vector# | MC1 vector$ | MN1 vector$ | MCLinker vector* | MNLinker vector* |
---|---|---|---|---|---|---|---|---|
AG | Tetramer | 492 | 505 | RDB18244 | ||||
mAG1 | Monomer | 492 | 505 | RDB18246 | RDB18247 | RDB18248 | ||
hmAG1 | Monomer | 492 | 505 | RDB18249 | RDB18250 | RDB18251 | RDB18252 | RDB18245 |
hmAG407 | Monomer | 407 | 498 | RDB18328 |
Protein | Resource (cat.num.) | Reference |
---|---|---|
mAzami Green (mAG1) | hmAG1/pRSETB (RDB15230). | A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. Karasawa, S. et al., J. Biol. Chem. 278 (36): 34167-34171 (2003). PubMed PMID 12819206. |
mAzami Green (mAG407) | hmAG407/pRSETB (RDB15229) | A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. Karasawa, S. et al., J. Biol. Chem. 278 (36): 34167-34171 (2003). PubMed PMID 12819206. |
External information
Azami Green (AG) in FPbase
mAzami Green (mAG) in FPbase
StayGold
Protein | Resource (cat.num.) | Reference |
---|---|---|
StayGold | (n1)StayGold/pRSET (RDB19605) (n1)StayGold(c4)/pRSET (RDB19606) (n1)oxStayGold/pRSET (RDB19607) (n1)oxStayGold(c4)/pRSET (RDB19608) tdStayGold/pcDNA3 (RDB19609) tdStayGold(c4)/pBS Coupler (RDB19610) tdoxStayGold/pcDNA3 (RDB19611) tdoxStayGold(c4)/pBS Coupler (RDB19612) |
A highly photostable and bright green fluorescent protein. Hirano, M. et al., Nat. Biotechnol. Nat. Biotechnol. 40 (7): 1132-1142 (2022). PMID 35468954. |
External information
StayGold in FPbase
mStayGold (QC2-6 FIQ)
Protein | Resource (cat.num.) | Reference |
---|---|---|
mStayGold | pRSETB/mStayGold (RDB20214) pRSETB/mStayGold(c4) (RDB20215) |
Ando, R. et al., StayGold variants for molecular fusion and membrane-targeting applications. Nat Methods. 2023 Nov 30. doi: 10.1038/s41592-023-02085-6. Epub ahead of print PMID 38036853. |
External information
mStayGold in FPbase
GFP
Protein | Resource (cat.num.) | Reference |
---|---|---|
GFP | pAGP (RDB19839) | Aequorea green fluorescent protein. Expression of the gene and fluorescence characteristics of the recombinant protein. Inouye, S. and Tsuji, F.I., FEBS Lett. 341 (2-3): 277-280 (1994). PMID 8137953. |
GFPS1 | pGFPS1 (RDB09057) pUC-GFPS1 (RDB09059) |
Cell-free protein synthesis system from Escherichia coli cells cultured at decreased temperatures improves productivity by decreasing DNA template degradation. Seki, E. et al., Anal. Biochem. 377 (2): 156-161. PMID 18375196. |
External information
GFP (avGFP) in FPbase
h2-3
Protein | Resource (cat.num.) | Reference |
---|---|---|
h2-3 | h2-3/pRSETb (RDB18219) | Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. Ando, R. et al., bioRxiv (2020) doi: https://doi.org/10.1101/2020.03.30.015156. |
External information
h2-3 in FPbase
Gamillus
Protein | Resource (cat.num.) | Reference |
---|---|---|
Gamillus | Gamillus/pcDNA3 (RDB19864) | Acid-Tolerant Monomeric GFP from Olindias formosa. Shinoda, H. et al., Cell Chem. Biol. 25 (3): 330-338.e7 (2018). PMID 29290624. |
External information
Gamillus in FPbase
Yellow
Achilles
Protein | Resource (cat.num.) | Reference |
---|---|---|
Achilles | Achilles/pRSETB (RDB15982) | Coupling delay controls synchronized oscillation in the segmentation clock. Yoshioka-Kobayashi, K. et al., Nature. 2020 Jan 8. doi: 10.1038/s41586-019-1882-z. [Epub ahead of print]. PMID 31915376. |
External information
Achilles in FPbase
Venus
Protein | Resource (cat.num.) | Reference |
---|---|---|
Venus | Venus/pCS2 (RDB15116) mVenus/pRSETB (RDB15117) |
A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nagai, T. et al., Nat. Biotechnol. 20 (1): 87-90 (2002). PubMed PMID 11753368. |
Venus | cp49Venus (RDB15120) cp145Venus (RDB15121) cp157Venus (RDB15122) cp173Venus (RDB15123) cp195Venus (RDB15124) cp229Venus (RDB15125) |
Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Nagai, T. et al., Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PubMed PMID 15247428. |
External information
Venus in FPbase
mVenus in FPbase
Cy11.5
Protein | Resource (cat.num.) | Reference |
---|---|---|
Cy11.5 | Cy11.5/pRSETB (RDB15706) | Concatenation of cyan and yellow fluorescent proteins for efficient resonance energy transfer. Shimozono, S. et al., Biochemistry 45 (20): 6267-6271 (2006). PubMed PMID 16700538. |
External information
Cy11.5 in FPbase
Orange
Kusabira Orange
橙色の蛍光を発し、pH安定性を示します。蛍光スペクトルがシャープであるため、通常組み合わせ難い赤色蛍光タンパク質とのマルチカラー蛍光イメージングが可能です。
Kusabira-Orange CoralHue™ Fluorescent Protein | ||||||||
Abbr. | Form | Excitation maxima (nm) | Emission maxima (nm) | S1 vector# | MC1 vector$ | MN1 vector$ | MCLinker vector* | MNLinker vector* |
---|---|---|---|---|---|---|---|---|
KO1 | Dimer | 548 | 561 | RDB18253 | ||||
mKO1 | Monomer | 548 | 559 | RDB18258 | RDB18259 | RDB18260 | ||
mKO2 | Monomer | 551 | 565 | RDB18299 | ||||
hKO1 | Dimer | 548 | 561 | RDB18254 | RDB18255 | RDB18256 | ||
hmKO1 | Monomer | 548 | 559 | RDB18261 | RDB18262 | RDB18263 | RDB18264 | RDB18257 |
hmKO2 | Monomer | 551 | 565 | RDB18300 | RDB18301 | RDB18302 | RDB18298 |
Protein | Resource (cat.num.) | Reference |
---|---|---|
Kusabira Orange (mKO1) | hmKO1/pRSETB (RDB15312) | Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312 (2004). PubMed PMID 15065984. |
Kusabira Orange (mKO2) | hmKO2/pRSETB (RDB15313) | Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312 (2004). PubMed PMID 15065984. |
Kusabira Orange kappa | hmKO-K/pRSETB (RDB15314) | Improving membrane voltage measurements using FRET with new fluorescent proteins. Tsutsui, H. et al., Nat. Methods 5 (8): 683-685 (2008). PubMed PMID 18622396. |
External information
KO1 in FPbase
mKO1 in FPbase
mKO2 in FPbase
mKOk in FPbase
Red
AzaleaB5
Protein | Resource (cat.num.) | Reference |
---|---|---|
AzaleaB5 | AzaleaB5/pRSETb (RDB18218) | Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. Ando, R. et al., bioRxiv (2020) doi: https://doi.org/10.1101/2020.03.30.015156. |
External information
AzaleaB5 in FPbase
Keima570 and Keima-Red
Keima570、Keima-Red: 前者は橙色(蛍光極大570 nm)、後者は赤色(蛍光極大620 nm)の蛍光を発します。共にストークシフト (励起ピークと蛍光ピークの差) が大きく、蛍光観察時に他色の影響を受けにくいため、マルチカラー蛍光イメージングに適しています。さらにレーザーなどの単波長励起による多波長測定にも適しています。
Keima570 CoralHue™ Fluorescent Protein | ||||||||
Abbr. | Form | Excitation maxima (nm) | Emission maxima (nm) | S1 vector# | MC1 vector$ | MN1 vector$ | MCLinker vector* | MNLinker vector* |
---|---|---|---|---|---|---|---|---|
dKeima570 | Dimer | 440 | 570 | RDB18294 | ||||
hdKeima570 | Dimer | 440 | 570 | RDB18295 | RDB18296 | RDB18293 |
Keima-Red
Keima-Red CoralHue™ Fluorescent Protein Kogure T et al. A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nature Biotechnology. 24: 577-581 (2006) (PMID: 16648840) |
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Abbr. | Form | Excitation maxima (nm) | Emission maxima (nm) | S1 vector# | MC1 vector$ | MN1 vector$ | MCLinker vector* | MNLinker vector* |
---|---|---|---|---|---|---|---|---|
dKeima-Red | Dimer | 440 | 616 | RDB18285 | ||||
mKeima-Red | Monomer | 440 | 620 | RDB18280 | RDB18281 | |||
hdKeima-Red | Dimer | 440 | 616 | RDB18286 | RDB18287 | RDB18284 | ||
hmKeima-Red | Monomer | 440 | 620 | RDB18282 | RDB18283 | RDB18279 |
Protein | Resource (cat.num.) | Reference |
---|---|---|
Keima-red | tdKeima/pRSETB (RDB15232) mKeima/pcDNA3 (RDB15233) dKeima/pRSETB (RDB15234) dKeima570/pRSETB (RDB15235) |
A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581 (2006). PubMed PMID 16648840. |
Keima-red | mKeima (h)/pCS2 (RDB15236) dKeima (h)/pCS2 (RDB15237) |
A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Katayama, H. et al., Chem. Biol. 18 (8): 1042-1052 (2011). PubMed PMID 21867919. |
External information
dKeima in FPbase
mKeima in FPbase
dKeima570 in FPbase
NpF2164g5_BV4
Protein | Resource (cat.num.) | Reference |
---|---|---|
NpF2164g5_BV4 | pCAG-EGFP-NpF2164g5_BV4 (RDB17331) | Rational conversion of chromophore selectivity of cyanobacteriochromes to accept mammalian intrinsic biliverdin. Fushimi, K., et al., Proc. Natl. Acad. Sci. U.S.A. 116 (17): 8301-8309 (2019). PubMed PMID 30948637. |
Photoconversion type
Kaede
緑色の蛍光を発します。紫外光(360 nm-410 nm)を照射することで、蛍光が赤色に不可逆に変化します。照射量に応じ、色を段階的に変えることができます。
Kaede CoralHue™ Fluorescent Protein Ando R et al. An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc Natl Acad Sci U S A. (2002) 99(20):12651-6. (PMID: 12271129) |
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Abbr. | Form | Excitation maxima (nm) | Emission maxima (nm) | S1 vector# | MC1 vector$ | MN1 vector$ | MCLinker vector* | MNLinker vector* |
---|---|---|---|---|---|---|---|---|
Kaede | Tetramer | 508/572 | 518/580 | RDB18241 | RDB18242 | RDB18243 |
Protein | Resource (cat.num.) | Reference |
---|---|---|
Kaede | Kaede/pRSETB (RDB15231) | An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Ando, R. et al., Proc. Natl. Acad. Sci. U.S.A. 99 (20): 12651-12656 (2002). PubMed PMID 12271129. |
External information
Kaede in FPbase
Kikume Green-Red
緑色の蛍光を発します。紫外光(360nm-410nm)を照射することで、蛍光が赤色に不可逆に変化します。Kaedeより高いフォトコンバージョン効率(紫外光による波長が変換する効率)を示します。
Kikume Green-Red CoralHue™ Fluorescent Protein Habuchi S et al. mKikGR, A monomeric photoswitchable fluorescent protein. PLoS ONE 3, e3944 (2008) (PMID: 19079591) Tsutsui H. et al. Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6, 233-238 (2005) (PMID: 15731765) |
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Abbr. | Form | Excitation maxima (nm) | Emission maxima (nm) | S1 vector# | MC1 vector$ | MN1 vector$ | MCLinker vector* | MNLinker vector* |
---|---|---|---|---|---|---|---|---|
KikGR1 | Tetramer | 507/583 | 517/593 | RDB18272 | RDB18273 | RDB18274 | ||
mKikGR1 | Monomer | 505/580 | 517/591 | RDB18304 | ||||
hKikGR1 | Tetramer | 507/583 | 517/593 | RDB18275 | RDB18276 | RDB18277 | RDB18278 | RDB18271 |
hmKikGR1 | Monomer | 505/580 | 517/591 | RDB18305 | RDB18303 |
Protein | Resource (cat.num.) | Reference |
---|---|---|
Kikume Green-Red | KikGR/pRSETB (RDB15259) | Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. Tsutsui, H. et al., EMBO Rep. 6 (3): 233-238 (2005). PubMed PMID 15731765. |
Kikume Green-Red | mKikGR/pRSETB (RDB15261) | mKikGR, a monomeric photoswitchable fluorescent protein. Habuchi, S. et al., PLoS One 3 (12): e3944 (2008). PubMed PMID 19079591. |
h41pkve6 (derivative of Kikume Green-Red) | Xpa (RDB15264) Xpa H62Q (RDB15265) |
A diffraction-quality protein crystal processed as an autophagic cargo. Tsutsui, H. et al., Mol. Cell 58 (1): 186-193 (2015). PubMed PMID 25773597. |
External information
Kikume Green-Red (KikGR1) in FPbase
mKikume Green-Red (mKikGR1) in FPbase
Xpa in FPbase
Photochromism type
SuperNova Green
Protein | Resource (cat.num.) | Reference |
---|---|---|
SuperNova Green | SuperNova Green/pRSETB (RDB19865) | Green monomeric photosensitizing fluorescent protein for photo-inducible protein inactivation and cell ablation. Riani, Y.D. et al., BMC Biol. 2018 Apr 30;16(1):50 PMID 29712573. |
External information
SuperNova Green in FPbase
Kohinoor 2.0
Protein | Resource (cat.num.) | Reference |
---|---|---|
Kohinoor 2.0 | Kohinoor 2.0/pcDNA3 (RDB19862) | A photoswitchable fluorescent protein for hours-time-lapse and sub-second-resolved super-resolution imaging. Wazawa, T. et al., Microscopy (Oxf). 70 (4): 340-352 (2021). PMID 33481018. |
External information
Kohinoor2.0 in FPbase
Dronpa Green
緑色の蛍光を発します。蛍光の明暗が可逆的に操作でき、503 nm付近の強い光をあてると蛍光が消え、390 nm付近の光をあてると蛍光が回復するフォトクロミック蛍光タンパク質です。Photoactivated localization microscopy (PALM)法による超解像顕微鏡観察に使用できます。
Dronpa Green CoralHue™ Fluorescent Protein Ando R et al. Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306, 1370-1373 (2004) (PMID: 15550670) |
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Abbr. | Form | Excitation maxima (nm) | Emission maxima (nm) | S1 vector# | MC1 vector$ | MN1 vector$ | MCLinker vector* | MNLinker vector* |
---|---|---|---|---|---|---|---|---|
DG1 | Monomer | 503 | 518 | RDB18267 | RDB18268 | RDB18269 | ||
hDG1 | Monomer | 503 | 518 | RDB18270 | RDB18266 | |||
DG3 | Monomer | 491 | 514 | RDB18297 |
Protein | Resource (cat.num.) | Reference |
---|---|---|
Dronpa-Green | Dronpa/pRSETB (RDB15256) 22G/pRSETB (RDB15255) |
Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Ando, R. et al., Science 306 (5700): 1370-1373 (2004). PubMed PMID 15550670. |
Dronpa-Green | Dronpa2/pRSETB (RDB15257) Dronpa3/pRSETB (RDB15258) |
Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants. Ando, R. et al., Biophys. J. 92 (12): L97-99 (2007). PubMed PMID 17384059. |
External information
Dronpa in FPbase
Dronpa-2 in FPbase
Dronpa-3 in FPbase
22G in FPbase
rsGamillus
Protein | Resource (cat.num.) | Reference |
---|---|---|
rsGamillus | rsGamillus-S/pcDNA3 (RDB19867) rsGamillus-F/pcDNA3 (RDB19868) |
Acid-Tolerant Reversibly Switchable Green Fluorescent Protein for Super-resolution Imaging under Acidic Conditions. Shinoda, H. et al., Cell Chem. Biol. 26 (10): 1469-1479.e6 (2019). PMID 31422907. |
SPOON
Protein | Resource (cat.num.) | Reference |
---|---|---|
SPOON | SPOON/pcDNA3 (RDB19863) | Spontaneously Blinking Fluorescent Protein for Simple Single Laser Super-Resolution Live Cell Imaging. Arai, Y. et al., ACS Chem. Biol. 13 (8): 1938-1943 (2018). PMID 29963852. |
External information
SPOON in FPbase
SuperNova
Protein | Resource (cat.num.) | Reference |
---|---|---|
SuperNova | SuperNova/pRSETB (RDB19866) | SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation. Takemoto, K. et al., Sci. Rep. 3: 2629 (2013). PMID 24043132. |
External information
SuperNova Red in FPbase
Timer type
Kusabira Green-Orange
Protein | Resource (cat.num.) | Reference |
---|---|---|
mKusabira Green Orange | mK-GO (RDB15244) | Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Tsuboi, T. et al., Mol. Biol. Cell. 21 (1): 87-94 (2010). PubMed PMID 19889833. |
External information
mKusabira Green Orange (mK-GO) in FPbase
Destabilized- and Secreted-FPs
Protein | Resource (cat.num.) | Reference |
---|---|---|
CFP | pdsCFP (RDB15993) (destabilized FP) | Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Ando, H. et al., Sci. Rep. 7 (1): 12642 (2017). PubMed PMID 28974737. |
GFP | pdsGFP (RDB15992) (destabilized FP) | Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Ando, H. et al., Sci. Rep. 7 (1): 12642 (2017). PubMed PMID 28974737. |
Venus | pdsVenus (RDB15994) (destabilized FP) | Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Ando, H. et al., Sci. Rep. 7 (1): 12642 (2017). PubMed PMID 28974737. |
Protein | Resource (cat.num.) | Reference |
---|---|---|
GFP | pMRX-bsr-ss-sfGFP (RDB19002) (secreted FP) | Knockout analysis of Rab6 effector proteins revealed the role of VPS52 in the secretory pathway. Homma, Y., Fukuda, M., Biochem0 Biophys. Res. Commun. 561: 151-157 (2021). PubMed PMID 34023780. |
FPs with signal sequence and tags
Protein | Resource (cat.num.) | Reference |
---|---|---|
GFP | pcDNA3.1-myc-GFP (RDB14213) (myc-tagged FP) | Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. Nishi, K. et al., RNA 19 (1): 17-35 (2013). PMID 23150874. (original). |
GFP | pCMFlag_EGFP (RDB06071) (FLAG-tagged FP) | unpublished |
Non-fluorescent chromoprotein
cjBlue
Protein | Resource (cat.num.) | Reference |
---|---|---|
cjBlue | cjBlue/pRSETB (RDB15242) cjBlue Y64L/pRSETB (RDB15243) |
Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. Chan, M.C. et al., J. Biol. Chem. 281 (49): 37813-37819 (2006). PubMed PMID 17028187. |
耐熱性GFP
- ウミサボテン由来の高い熱安定性を有する GFPです。100℃、10 分の加熱処理後でも充分な蛍光を有しています。
参考文献. 熱安定性を有する蛍光タンパク質およびそれを利用する方法 特開2014-6094725℃ 70℃ 100℃ A. victoria由来GFP 1072 1087 629 WT 4770 4229 703 Th1wt 8067 7236 2162 Th2wt 7668 7525 3711 上段、加熱処理前。下段、100℃ 10分加熱後。
それぞれ室温で観察。図の蛍光強度を数値化したもの。 (注) 画像、ならびにデータは、オリンパス株式会社から提供を受け、編集して掲載。
Catalog no. Name of clone Characteristic RDB14364 pCoGFP-Th1wt wild type RDB14366 pCoGFP-Th2wt wild type RDB14365 pCoGFP-Th1co Optimize codons for transfection into mammalian cells RDB14367 pCoGFP-Th2co Optimize codons for transfection into mammalian cells
pH感受性GFP
pH に応じて青色 (456nm) から緑色 (507nm) に変化する蛍光を有する ウミサボテン由来のGFP です。哺乳動物細胞内における pH センサーとして活用できます。
Ogoh, K. et al. Dual-color-emitting green fluorescent protein from the sea cactus Cavernularia obesa and its use as a pH indicator for fluorescence microscopy. Luminescence 28 (4): 582-591, 2013.
362nm で励起した各種 pH 感受性 GFP の pH4 – 11 における青色から緑色への蛍光推移をイメージしたイラスト。詳しくは、Ogoh, K. et al., 2013 の Figure 6 をご覧ください。 |
Catalog no. | Name of clone | Characteristic | misc. |
---|---|---|---|
RDB14368 | pCoGFP-wt | wild type | GFP cDNA is cloned in pUC19 vector. |
RDB14369 | pCoGFP-mam | From blue to green at pH 5 – 6 | |
RDB14370 | pCoGFP-V0 | From blue to green at pH 5 – 6 | |
RDB14371 | pCoGFP-V1 | From blue to green at pH 6 – 7 | |
RDB14372 | pCoGFP-V2 | From blue to green at pH 7 – 8 | |
RDB14373 | pCoGFP-V3 | From blue to green at pH 9 – 10 | |
RDB14374 | pCoGFP-V4 | From blue to green at pH 9 – 10 |
(注) 画像、ならびにデータは、オリンパス株式会社から提供を受け、編集して掲載。
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Arranged vector
Catalog num. Clone name Description |
Antibiotic/visual marker | Promoter | Antibiotics (bacteria) |
---|---|---|---|
RDB10118 pmPGKpacECFPpA Expression vector of pac-ECFP with mPGK promoter. |
Puro & ECFP | CAG | Amp |
RDB06071 pCMFlag_EGFP Expression vector of GFP, with FLAG-tag, control vector. |
FLAG-EGFP | CMV | Amp |
RDB07071 pdxEFFlag_EGFPit2 Expression vector of EGFP. |
FLAG-EGFP | EF-1 alpha | Amp |
RDB07073 pdxEFFlag_EGFPit2 Expression vector of EGFP. |
FLAG-EGFP | EF-1 alpha | Amp |
RDB08525 pPyCAG-EGFP-IP Expression clone of GFP under the control of CAG promoter, puromycin resistance. |
Puro & EGFP | CAG | Amp |
RDB08526 pPyCAG-EGFP-IZ Expression clone of GFP under the control of CAG promoter, zeocin resistance. |
Bleo(Zeo) & EGFP | CAG | Amp |
RDB08527 pPyCAG-EGFP-ID Expression clone of GFP under the control of CAG promoter, HisD resistance. |
HisD & EGFP | CAG | Amp |
RDB08528 pPyCAG-EGFP-IB Expression clone of GFP under the control of CAG promoter, Blasticidin resistance. |
BSD & EGFP | CAG | Amp |
RDB08529 pBRPyCAG-EGFP-IH Expression clone of GFP under the control of CAG promoter, hygromycin resistance |
Hygro & EGFP | CAG | Amp |
RDB08530 pBRPyCAG-EGFP-IN Expression clone of GFP under the control of CAG promoter, neomycin resistance |
Neo & EGFP | CAG | Amp |
RDB03006 pCAEGFP Expression vector of EGFP driven by CA promoter. |
EGFP | CAG | Amp |
References
- FPbase
- Table of Fluorochromes (Salk Institute for Biological Studies)
(GRP0050j 2016.11.16 N.N.)
2023.12.18