蛍光タンパク質リソース

Fluorescent protein resource

Ultramarine

Sirius

Protein	Resource (cat.num.)	Reference
Sirius	Sirius/pcDNA3 (RDB19875)	An ultramarine fluorescent protein with increased photostability and pH insensitivity. Tomosugi, W. et al., Nat. Methods. 6 (5): 351-353 (2009). PMID 19349978.

External information
Sirius in FPbase

Cyan

Kusabira Cyan

青緑色の蛍光を発します。ECFPに比べて黄色への蛍光スペクトルの重なりが少ないです。

Abbr.	Form	Excitation maxima (nm)	Emission maxima (nm)	S1 vector^#	MC1 vector^$	MN1 vector^$	MCLinker vector^*	MNLinker vector^*
Kusabira-Cyan CoralHue™ Fluorescent Protein Kikuchi A et al. Crystal structure of a new cyan fluorescent protein and its hue-shifted variants. Biochemistry 48, 5276-83 (2009) (PMID: 19402703)
KCy1	Dimer	453	486	RDB18310

Midoriishi Cyan

青緑色の蛍光を発します。ECFPに比べて黄色への蛍光スペクトルの重なりが少ないです。

Abbr.	Form	Excitation maxima (nm)	Emission maxima (nm)	S1 vector^#	MC1 vector^$	MN1 vector^$	MCLinker vector^*	MNLinker vector^*
Midoriishi-Cyan CoralHue™ Fluorescent Protein Karasawa S et al. Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem J. 381, 307-312 (2004) (PMID: 15065984)
MiCy1	Dimer	472	495	RDB18265
mMiCy1	Monomer	470	496	RDB18289
hmMiCy1	Monomer	470	496		RDB18290	RDB18291	RDB18292	RDB18288

Protein	Resource (cat.num.)	Reference
mMidoriishi-Cyan	mMiCy1/pRSETB (RDB15241)	Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312 (2004). PubMed PMID 15065984.

External information
Midoriishi Cyan (MiCy) in FPbase
mMidoriishi Cyan (mMiCy) in FPbase

Green

Umikinoko Green

鮮やかな緑色の蛍光を発し、pH安定性を示します。

Abbr.	Form	Excitation maxima (nm)	Emission maxima (nm)	S1 vector^#	MC1 vector^$	MN1 vector^$
Umikinoko-Green CoralHue™ Fluorescent Protein Tsutsui H et al. Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat Methods. 5, 683-685 (2008) (PMID: 18622396)
mUKG1	Monomer	483	499	RDB18306
hmUKG1	Monomer	483	499	RDB18307	RDB18308	RDB18309

External information
mUmikinoko-Green (mUkG) in FPbase

Azami Green

緑色の蛍光を発する蛍光タンパク質です。

Abbr.	Form	Excitation maxima (nm)	Emission maxima (nm)	S1 vector^#	MC1 vector^$	MN1 vector^$	MCLinker vector^*	MNLinker vector^*
Azami-Green CoralHue™ Fluorescent Protein Karasawa S et al. A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J Biol Chem. 278, 34167-34171 (2003) (PMID: 12819206)
AG	Tetramer	492	505	RDB18244
mAG1	Monomer	492	505	RDB18246	RDB18247	RDB18248
hmAG1	Monomer	492	505	RDB18249	RDB18250	RDB18251	RDB18252	RDB18245
hmAG407	Monomer	407	498	RDB18328

Protein	Resource (cat.num.)	Reference
mAzami Green (mAG1)	hmAG1/pRSETB (RDB15230).	A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. Karasawa, S. et al., J. Biol. Chem. 278 (36): 34167-34171 (2003). PubMed PMID 12819206.
mAzami Green (mAG407)	hmAG407/pRSETB (RDB15229)	A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. Karasawa, S. et al., J. Biol. Chem. 278 (36): 34167-34171 (2003). PubMed PMID 12819206.

External information
Azami Green (AG) in FPbase
mAzami Green (mAG) in FPbase

StayGold

Protein	Resource (cat.num.)	Reference
StayGold	(n1)StayGold/pRSET (RDB19605) (n1)StayGold(c4)/pRSET (RDB19606) (n1)oxStayGold/pRSET (RDB19607) (n1)oxStayGold(c4)/pRSET (RDB19608) tdStayGold/pcDNA3 (RDB19609) tdStayGold(c4)/pBS Coupler (RDB19610) tdoxStayGold/pcDNA3 (RDB19611) tdoxStayGold(c4)/pBS Coupler (RDB19612)	A highly photostable and bright green fluorescent protein. Hirano, M. et al., Nat. Biotechnol. Nat. Biotechnol. 40 (7): 1132-1142 (2022). PMID 35468954.

External information
StayGold in FPbase

mStayGold (QC2-6 FIQ)

Protein	Resource (cat.num.)	Reference
mStayGold	pRSETB/mStayGold (RDB20214) pRSETB/mStayGold(c4) (RDB20215) pBS Coupler4/td8ox2StayGold (c4) (RDB20226) pBS Coupler1/td8ox2StayGold (RDB20227)	Ando, R. et al., StayGold variants for molecular fusion and membrane-targeting applications. Nat Methods. 2023 Nov 30. doi: 10.1038/s41592-023-02085-6. Epub ahead of print PMID 38036853.

External information
mStayGold in FPbase

GFP

Protein	Resource (cat.num.)	Reference
GFP	pAGP (RDB19839)	Aequorea green fluorescent protein. Expression of the gene and fluorescence characteristics of the recombinant protein. Inouye, S. and Tsuji, F.I., FEBS Lett. 341 (2-3): 277-280 (1994). PMID 8137953.
GFPS1	pGFPS1 (RDB09057) pUC-GFPS1 (RDB09059)	Cell-free protein synthesis system from Escherichia coli cells cultured at decreased temperatures improves productivity by decreasing DNA template degradation. Seki, E. et al., Anal. Biochem. 377 (2): 156-161. PMID 18375196.

External information
GFP (avGFP) in FPbase

h2-3

Protein	Resource (cat.num.)	Reference
h2-3	h2-3/pRSETb (RDB18219)	Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. Ando, R. et al., bioRxiv (2020) doi: https://doi.org/10.1101/2020.03.30.015156.

External information
h2-3 in FPbase

Gamillus

Protein	Resource (cat.num.)	Reference
Gamillus	Gamillus/pcDNA3 (RDB19864)	Acid-Tolerant Monomeric GFP from Olindias formosa. Shinoda, H. et al., Cell Chem. Biol. 25 (3): 330-338.e7 (2018). PMID 29290624.

External information
Gamillus in FPbase

Yellow

Achilles

Protein	Resource (cat.num.)	Reference
Achilles	Achilles/pRSETB (RDB15982)	Coupling delay controls synchronized oscillation in the segmentation clock. Yoshioka-Kobayashi, K. et al., Nature. 2020 Jan 8. doi: 10.1038/s41586-019-1882-z. [Epub ahead of print]. PMID 31915376.

External information
Achilles in FPbase

Venus

Protein	Resource (cat.num.)	Reference
Venus	Venus/pCS2 (RDB15116) mVenus/pRSETB (RDB15117)	A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nagai, T. et al., Nat. Biotechnol. 20 (1): 87-90 (2002). PubMed PMID 11753368.
Venus	cp49Venus (RDB15120) cp145Venus (RDB15121) cp157Venus (RDB15122) cp173Venus (RDB15123) cp195Venus (RDB15124) cp229Venus (RDB15125)	Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Nagai, T. et al., Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PubMed PMID 15247428.

External information
Venus in FPbase
mVenus in FPbase

Cy11.5

Protein	Resource (cat.num.)	Reference
Cy11.5	Cy11.5/pRSETB (RDB15706)	Concatenation of cyan and yellow fluorescent proteins for efficient resonance energy transfer. Shimozono, S. et al., Biochemistry 45 (20): 6267-6271 (2006). PubMed PMID 16700538.

External information
Cy11.5 in FPbase

Orange

Kusabira Orange

橙色の蛍光を発し、pH安定性を示します。蛍光スペクトルがシャープであるため、通常組み合わせ難い赤色蛍光タンパク質とのマルチカラー蛍光イメージングが可能です。

Abbr.	Form	Excitation maxima (nm)	Emission maxima (nm)	S1 vector^#	MC1 vector^$	MN1 vector^$	MCLinker vector^*	MNLinker vector^*
Kusabira-Orange CoralHue™ Fluorescent Protein Karasawa S et al. Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem J. 381, 307-312 (2004) (PMID: 15065984)
KO1	Dimer	548	561	RDB18253
mKO1	Monomer	548	559	RDB18258	RDB18259	RDB18260
mKO2	Monomer	551	565	RDB18299
hKO1	Dimer	548	561	RDB18254	RDB18255	RDB18256
hmKO1	Monomer	548	559	RDB18261	RDB18262	RDB18263	RDB18264	RDB18257
hmKO2	Monomer	551	565		RDB18300	RDB18301	RDB18302	RDB18298

Protein	Resource (cat.num.)	Reference
Kusabira Orange (mKO1)	hmKO1/pRSETB (RDB15312)	Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312 (2004). PubMed PMID 15065984.
Kusabira Orange (mKO2)	hmKO2/pRSETB (RDB15313)	Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312 (2004). PubMed PMID 15065984.
Kusabira Orange kappa	hmKO-K/pRSETB (RDB15314)	Improving membrane voltage measurements using FRET with new fluorescent proteins. Tsutsui, H. et al., Nat. Methods 5 (8): 683-685 (2008). PubMed PMID 18622396.

External information
KO1 in FPbase
mKO1 in FPbase
mKO2 in FPbase
mKOk in FPbase

Red

AzaleaB5

Protein	Resource (cat.num.)	Reference
AzaleaB5	AzaleaB5/pRSETb (RDB18218)	Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. Ando, R. et al., bioRxiv (2020) doi: https://doi.org/10.1101/2020.03.30.015156.

External information
AzaleaB5 in FPbase

Keima570 and Keima-Red

Keima570、Keima-Red: 前者は橙色(蛍光極大570 nm)、後者は赤色(蛍光極大620 nm)の蛍光を発します。共にストークシフト (励起ピークと蛍光ピークの差) が大きく、蛍光観察時に他色の影響を受けにくいため、マルチカラー蛍光イメージングに適しています。さらにレーザーなどの単波長励起による多波長測定にも適しています。

Abbr.	Form	Excitation maxima (nm)	Emission maxima (nm)	S1 vector^#	MCLinker vector^*	MNLinker vector^*
Keima570 CoralHue™ Fluorescent Protein Kogure T et al. A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nature Biotechnology. 24: 577-581 (2006) (PMID: 16648840)
dKeima570	Dimer	440	570	RDB18294
hdKeima570	Dimer	440	570	RDB18295	RDB18296	RDB18293

Keima-Red

Abbr.	Form	Excitation maxima (nm)	Emission maxima (nm)	S1 vector^#	MN1 vector^$	MCLinker vector^*	MNLinker vector^*
Keima-Red CoralHue™ Fluorescent Protein Kogure T et al. A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nature Biotechnology. 24: 577-581 (2006) (PMID: 16648840)
dKeima-Red	Dimer	440	616	RDB18285
mKeima-Red	Monomer	440	620	RDB18280	RDB18281
hdKeima-Red	Dimer	440	616	RDB18286		RDB18287	RDB18284
hmKeima-Red	Monomer	440	620	RDB18282		RDB18283	RDB18279

Protein	Resource (cat.num.)	Reference
Keima-red	tdKeima/pRSETB (RDB15232) mKeima/pcDNA3 (RDB15233) dKeima/pRSETB (RDB15234) dKeima570/pRSETB (RDB15235)	A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581 (2006). PubMed PMID 16648840.
Keima-red	mKeima (h)/pCS2 (RDB15236) dKeima (h)/pCS2 (RDB15237)	A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Katayama, H. et al., Chem. Biol. 18 (8): 1042-1052 (2011). PubMed PMID 21867919.

External information
dKeima in FPbase
mKeima in FPbase
dKeima570 in FPbase

NpF2164g5_BV4

Protein	Resource (cat.num.)	Reference
NpF2164g5_BV4	pCAG-EGFP-NpF2164g5_BV4 (RDB17331)	Rational conversion of chromophore selectivity of cyanobacteriochromes to accept mammalian intrinsic biliverdin. Fushimi, K., et al., Proc. Natl. Acad. Sci. U.S.A. 116 (17): 8301-8309 (2019). PubMed PMID 30948637.

Photoconversion type

Kaede

緑色の蛍光を発します。紫外光(360 nm-410 nm)を照射することで、蛍光が赤色に不可逆に変化します。照射量に応じ、色を段階的に変えることができます。

Abbr.	Form	Excitation maxima (nm)	Emission maxima (nm)	S1 vector^#	MC1 vector^$	MN1 vector^$	MCLinker vector^*	MNLinker vector^*
Kaede CoralHue™ Fluorescent Protein Ando R et al. An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc Natl Acad Sci U S A. (2002) 99(20):12651-6. (PMID: 12271129)
Kaede	Tetramer	508/572	518/580	RDB18241	RDB18242	RDB18243

Protein	Resource (cat.num.)	Reference
Kaede	Kaede/pRSETB (RDB15231)	An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Ando, R. et al., Proc. Natl. Acad. Sci. U.S.A. 99 (20): 12651-12656 (2002). PubMed PMID 12271129.

External information
Kaede in FPbase

Kikume Green-Red

緑色の蛍光を発します。紫外光(360nm-410nm)を照射することで、蛍光が赤色に不可逆に変化します。Kaedeより高いフォトコンバージョン効率(紫外光による波長が変換する効率）を示します。

Abbr.	Form	Excitation maxima (nm)	Emission maxima (nm)	S1 vector^#	MC1 vector^$	MN1 vector^$	MCLinker vector^*	MNLinker vector^*
Kikume Green-Red CoralHue™ Fluorescent Protein Habuchi S et al. mKikGR, A monomeric photoswitchable fluorescent protein. PLoS ONE 3, e3944 (2008) (PMID: 19079591) Tsutsui H. et al. Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6, 233-238 (2005) (PMID: 15731765)
KikGR1	Tetramer	507/583	517/593	RDB18272	RDB18273	RDB18274
mKikGR1	Monomer	505/580	517/591	RDB18304
hKikGR1	Tetramer	507/583	517/593	RDB18275	RDB18276	RDB18277	RDB18278	RDB18271
hmKikGR1	Monomer	505/580	517/591				RDB18305	RDB18303

Protein	Resource (cat.num.)	Reference
Kikume Green-Red	KikGR/pRSETB (RDB15259)	Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. Tsutsui, H. et al., EMBO Rep. 6 (3): 233-238 (2005). PubMed PMID 15731765.
Kikume Green-Red	mKikGR/pRSETB (RDB15261)	mKikGR, a monomeric photoswitchable fluorescent protein. Habuchi, S. et al., PLoS One 3 (12): e3944 (2008). PubMed PMID 19079591.
h41pkve6 (derivative of Kikume Green-Red)	Xpa (RDB15264) Xpa H62Q (RDB15265)	A diffraction-quality protein crystal processed as an autophagic cargo. Tsutsui, H. et al., Mol. Cell 58 (1): 186-193 (2015). PubMed PMID 25773597.

External information
Kikume Green-Red (KikGR1) in FPbase
mKikume Green-Red (mKikGR1) in FPbase
Xpa in FPbase

Photochromism type

SuperNova Green

Protein	Resource (cat.num.)	Reference
SuperNova Green	SuperNova Green/pRSETB (RDB19865)	Green monomeric photosensitizing fluorescent protein for photo-inducible protein inactivation and cell ablation. Riani, Y.D. et al., BMC Biol. 2018 Apr 30;16(1):50 PMID 29712573.

External information
SuperNova Green in FPbase

Kohinoor 2.0

Protein	Resource (cat.num.)	Reference
Kohinoor 2.0	Kohinoor 2.0/pcDNA3 (RDB19862)	A photoswitchable fluorescent protein for hours-time-lapse and sub-second-resolved super-resolution imaging. Wazawa, T. et al., Microscopy (Oxf). 70 (4): 340-352 (2021). PMID 33481018.

External information
Kohinoor2.0 in FPbase

Dronpa Green

緑色の蛍光を発します。蛍光の明暗が可逆的に操作でき、503 nm付近の強い光をあてると蛍光が消え、390 nm付近の光をあてると蛍光が回復するフォトクロミック蛍光タンパク質です。Photoactivated localization microscopy (PALM)法による超解像顕微鏡観察に使用できます。

Abbr.	Form	Excitation maxima (nm)	Emission maxima (nm)	S1 vector^#	MC1 vector^$	MN1 vector^$	MCLinker vector^*	MNLinker vector^*
Dronpa Green CoralHue™ Fluorescent Protein Ando R et al. Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306, 1370-1373 (2004) (PMID: 15550670)
DG1	Monomer	503	518	RDB18267	RDB18268	RDB18269
hDG1	Monomer	503	518				RDB18270	RDB18266
DG3	Monomer	491	514	RDB18297

Protein	Resource (cat.num.)	Reference
Dronpa-Green	Dronpa/pRSETB (RDB15256) 22G/pRSETB (RDB15255)	Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Ando, R. et al., Science 306 (5700): 1370-1373 (2004). PubMed PMID 15550670.
Dronpa-Green	Dronpa2/pRSETB (RDB15257) Dronpa3/pRSETB (RDB15258)	Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants. Ando, R. et al., Biophys. J. 92 (12): L97-99 (2007). PubMed PMID 17384059.

External information
Dronpa in FPbase
Dronpa-2 in FPbase
Dronpa-3 in FPbase
22G in FPbase

rsGamillus

Protein	Resource (cat.num.)	Reference
rsGamillus	rsGamillus-S/pcDNA3 (RDB19867) rsGamillus-F/pcDNA3 (RDB19868)	Acid-Tolerant Reversibly Switchable Green Fluorescent Protein for Super-resolution Imaging under Acidic Conditions. Shinoda, H. et al., Cell Chem. Biol. 26 (10): 1469-1479.e6 (2019). PMID 31422907.

SPOON

Protein	Resource (cat.num.)	Reference
SPOON	SPOON/pcDNA3 (RDB19863)	Spontaneously Blinking Fluorescent Protein for Simple Single Laser Super-Resolution Live Cell Imaging. Arai, Y. et al., ACS Chem. Biol. 13 (8): 1938-1943 (2018). PMID 29963852.

External information
SPOON in FPbase

SuperNova

Protein	Resource (cat.num.)	Reference
SuperNova	SuperNova/pRSETB (RDB19866)	SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation. Takemoto, K. et al., Sci. Rep. 3: 2629 (2013). PMID 24043132.

External information
SuperNova Red in FPbase

Timer type

Kusabira Green-Orange

Protein	Resource (cat.num.)	Reference
mKusabira Green Orange	mK-GO (RDB15244)	Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Tsuboi, T. et al., Mol. Biol. Cell. 21 (1): 87-94 (2010). PubMed PMID 19889833.

External information
mKusabira Green Orange (mK-GO) in FPbase

Destabilized- and Secreted-FPs

Protein	Resource (cat.num.)	Reference
CFP	pdsCFP (RDB15993) (destabilized FP)	Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Ando, H. et al., Sci. Rep. 7 (1): 12642 (2017). PubMed PMID 28974737.
GFP	pdsGFP (RDB15992) (destabilized FP)	Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Ando, H. et al., Sci. Rep. 7 (1): 12642 (2017). PubMed PMID 28974737.
Venus	pdsVenus (RDB15994) (destabilized FP)	Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Ando, H. et al., Sci. Rep. 7 (1): 12642 (2017). PubMed PMID 28974737.

Protein	Resource (cat.num.)	Reference
GFP	pMRX-bsr-ss-sfGFP (RDB19002) (secreted FP)	Knockout analysis of Rab6 effector proteins revealed the role of VPS52 in the secretory pathway. Homma, Y., Fukuda, M., Biochem0 Biophys. Res. Commun. 561: 151-157 (2021). PubMed PMID 34023780.

FPs with signal sequence and tags

Protein	Resource (cat.num.)	Reference
GFP	pcDNA3.1-myc-GFP (RDB14213) (myc-tagged FP)	Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. Nishi, K. et al., RNA 19 (1): 17-35 (2013). PMID 23150874. (original).
GFP	pCMFlag_EGFP (RDB06071) (FLAG-tagged FP)	unpublished

Non-fluorescent chromoprotein

cjBlue

Protein	Resource (cat.num.)	Reference
cjBlue	cjBlue/pRSETB (RDB15242) cjBlue Y64L/pRSETB (RDB15243)	Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. Chan, M.C. et al., J. Biol. Chem. 281 (49): 37813-37819 (2006). PubMed PMID 17028187.

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耐熱性GFP

ウミサボテン由来の高い熱安定性を有する GFPです。100℃、10 分の加熱処理後でも充分な蛍光を有しています。
参考文献. 熱安定性を有する蛍光タンパク質およびそれを利用する方法　特開2014-60947

		25℃	70℃	100℃
	A. victoria由来GFP	1072	1087	629
	WT	4770	4229	703
	Th1wt	8067	7236	2162
	Th2wt	7668	7525	3711
上段、加熱処理前。下段、100℃ 10分加熱後。それぞれ室温で観察。	図の蛍光強度を数値化したもの。

(注) 画像、ならびにデータは、オリンパス株式会社から提供を受け、編集して掲載。

Catalog no.	Name of clone	Characteristic
RDB14364	pCoGFP-Th1wt	wild type
RDB14366	pCoGFP-Th2wt	wild type
RDB14365	pCoGFP-Th1co	Optimize codons for transfection into mammalian cells
RDB14367	pCoGFP-Th2co	Optimize codons for transfection into mammalian cells

pH感受性GFP

pH に応じて青色 (456nm) から緑色 (507nm) に変化する蛍光を有するウミサボテン由来のGFP です。哺乳動物細胞内における pH センサーとして活用できます。
Ogoh, K. et al. Dual-color-emitting green fluorescent protein from the sea cactus Cavernularia obesa and its use as a pH indicator for fluorescence microscopy. Luminescence 28 (4): 582-591, 2013.

362nm で励起した各種 pH 感受性 GFP の pH4 – 11 における青色から緑色への蛍光推移をイメージしたイラスト。詳しくは、Ogoh, K. et al., 2013 の Figure 6 をご覧ください。

Catalog no.	Name of clone	Characteristic	misc.
RDB14368	pCoGFP-wt	wild type	GFP cDNA is cloned in pUC19 vector.
RDB14369	pCoGFP-mam	From blue to green at pH 5 – 6
RDB14370	pCoGFP-V0	From blue to green at pH 5 – 6
RDB14371	pCoGFP-V1	From blue to green at pH 6 – 7
RDB14372	pCoGFP-V2	From blue to green at pH 7 – 8
RDB14373	pCoGFP-V3	From blue to green at pH 9 – 10
RDB14374	pCoGFP-V4	From blue to green at pH 9 – 10

(注) 画像、ならびにデータは、オリンパス株式会社から提供を受け、編集して掲載。
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Arranged vector

Catalog num. Clone name Description	Antibiotic/visual marker	Promoter	Antibiotics (bacteria)
RDB10118 pmPGKpacECFPpA Expression vector of pac-ECFP with mPGK promoter.	Puro & ECFP	CAG	Amp
RDB06071 pCMFlag_EGFP Expression vector of GFP, with FLAG-tag, control vector.	FLAG-EGFP	CMV	Amp
RDB07071 pdxEFFlag_EGFPit2 Expression vector of EGFP.	FLAG-EGFP	EF-1 alpha	Amp
RDB07073 pdxEFFlag_EGFPit2 Expression vector of EGFP.	FLAG-EGFP	EF-1 alpha	Amp
RDB08525 pPyCAG-EGFP-IP Expression clone of GFP under the control of CAG promoter, puromycin resistance.	Puro & EGFP	CAG	Amp
RDB08526 pPyCAG-EGFP-IZ Expression clone of GFP under the control of CAG promoter, zeocin resistance.	Bleo(Zeo) & EGFP	CAG	Amp
RDB08527 pPyCAG-EGFP-ID Expression clone of GFP under the control of CAG promoter, HisD resistance.	HisD & EGFP	CAG	Amp
RDB08528 pPyCAG-EGFP-IB Expression clone of GFP under the control of CAG promoter, Blasticidin resistance.	BSD & EGFP	CAG	Amp
RDB08529 pBRPyCAG-EGFP-IH Expression clone of GFP under the control of CAG promoter, hygromycin resistance	Hygro & EGFP	CAG	Amp
RDB08530 pBRPyCAG-EGFP-IN Expression clone of GFP under the control of CAG promoter, neomycin resistance	Neo & EGFP	CAG	Amp
RDB03006 pCAEGFP Expression vector of EGFP driven by CA promoter.	EGFP	CAG	Amp

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References

FPbase
Table of Fluorochromes (Salk Institute for Biological Studies)

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(GRP0050j 2016.11.16 N.N.)

2024.12.04

Fluorescent protein resource

Ultramarine

Sirius

Cyan

Kusabira Cyan

Midoriishi Cyan

Green

Umikinoko Green

Azami Green

StayGoldnewImage(2022,5,13)

mStayGold (QC2-6 FIQ)newImage(2023,12,18)

GFP

h2-3

Gamillus

Yellow

Achilles

Venus

Cy11.5

Orange

Kusabira Orange

Red

AzaleaB5

Keima570 and Keima-Red

Keima-Red

NpF2164g5_BV4

Photoconversion type

Kaede

Kikume Green-Red

Photochromism type

SuperNova Green

Kohinoor 2.0

Dronpa Green

rsGamillus

SPOON

SuperNova

Timer type

Kusabira Green-Orange

Destabilized- and Secreted-FPs

FPs with signal sequence and tags

Non-fluorescent chromoprotein

cjBlue

耐熱性GFP

pH感受性GFP

Arranged vector

References

StayGold

mStayGold (QC2-6 FIQ)