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蛍光タンパク質リソース

Fluorescent protein resource

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Cyan | Green | Yellow | Orange | Red
 
photoconversion type | photochromism type | Non-fluorescent chromoprotein
 
Tools | 耐熱性GFP | pH感受性GFP | Sensor & Visualization | Luminescent protein

Cyan

Kusabira Cyan

青緑色の蛍光を発します。ECFPに比べて黄色への蛍光スペクトルの重なりが少ないです。

Kusabira-Cyan CoralHue™ Fluorescent Protein
Kikuchi A et al. Crystal structure of a new cyan fluorescent protein and its hue-shifted variants. Biochemistry 48, 5276-83 (2009) (PMID: 19402703)
Abbr. Form Excitation maxima (nm) Emission maxima (nm) S1 vector# MC1 vector$ MN1 vector$ MCLinker vector* MNLinker vector*
KCy1 Dimer 453 486 RDB18310        

Midoriishi Cyan

青緑色の蛍光を発します。ECFPに比べて黄色への蛍光スペクトルの重なりが少ないです。

Midoriishi-Cyan CoralHue™ Fluorescent Protein
Karasawa S et al. Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem J. 381, 307-312 (2004) (PMID: 15065984)
Abbr. Form Excitation maxima (nm) Emission maxima (nm) S1 vector# MC1 vector$ MN1 vector$ MCLinker vector* MNLinker vector*
MiCy1 Dimer 472 495 RDB18265        
mMiCy1 Monomer 470 496 RDB18289        
hmMiCy1 Monomer 470 496   RDB18290 RDB18291 RDB18292 RDB18288
Protein Resource (cat.num.) Reference
Midoriishi-Cyan mMiCy1/pRSETB (RDB15241) Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer.
Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312 (2004). PubMed PMID 15065984.

CFP

Protein Resource (cat.num.) Reference
CFP pdsCFP (RDB15993) (destabilized FP) Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells.
Ando, H. et al., Sci. Rep. 7 (1): 12642 (2017). PubMed PMID 28974737.

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Green

Umikinoko Green

鮮やかな緑色の蛍光を発し、pH安定性を示します。

Umikinoko-Green CoralHue™ Fluorescent Protein
Tsutsui H et al. Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat Methods. 5, 683-685 (2008) (PMID: 18622396)
Abbr. Form Excitation maxima (nm) Emission maxima (nm) S1 vector# MC1 vector$ MN1 vector$ MCLinker vector* MNLinker vector*
mUKG1 Monomer 483 499 RDB18306        
hmUKG1 Monomer 483 499 RDB18307 RDB18308 RDB18309    

Azami Green

緑色の蛍光を発する蛍光タンパク質です。

Azami-Green CoralHue™ Fluorescent Protein
Karasawa S et al. A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J Biol Chem. 278, 34167-34171 (2003) (PMID: 12819206)
Abbr. Form Excitation maxima (nm) Emission maxima (nm) S1 vector# MC1 vector$ MN1 vector$ MCLinker vector* MNLinker vector*
AG Tetramer 492 505 RDB18244        
mAG1 Monomer 492 505 RDB18246 RDB18247 RDB18248    
hmAG1 Monomer 492 505 RDB18249 RDB18250 RDB18251 RDB18252 RDB18245
hmAG407 Monomer 407 498 RDB18328        
Protein Resource (cat.num.) Reference
Azami Green hmAG407/pRSETB (RDB15229)
hmAG1/pRSETB (RDB15230). 		A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling.
Karasawa, S. et al., J. Biol. Chem. 278 (36): 34167-34171 (2003). PubMed PMID 12819206.

h2-3

Protein Resource (cat.num.) Reference
h2-3 h2-3/pRSETb (RDB18219) Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression.
Ando, R. et al., bioRxiv (2020) doi: https://doi.org/10.1101/2020.03.30.015156.

GFP

Protein Resource (cat.num.) Reference
GFP pdsGFP (RDB15992) (destabilized FP) Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells.
Ando, H. et al., Sci. Rep. 7 (1): 12642 (2017). PubMed PMID 28974737.

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Yellow

Achilles

Protein Resource (cat.num.) Reference
Achilles Achilles/pRSETB (RDB15982) Coupling delay controls synchronized oscillation in the segmentation clock.
Yoshioka-Kobayashi, K. et al., Nature. 2020 Jan 8. doi: 10.1038/s41586-019-1882-z. [Epub ahead of print]. PMID 31915376.

Venus

Protein Resource (cat.num.) Reference
Venus Venus/pCS2 (RDB15116)
mVenus/pRSETB (RDB15117) 		A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications.
Nagai, T. et al., Nat. Biotechnol. 20 (1): 87-90 (2002). PubMed PMID 11753368.
Venus cp49Venus (RDB15120)
cp145Venus (RDB15121)
cp157Venus (RDB15122)
cp173Venus (RDB15123)
cp195Venus (RDB15124)
cp229Venus (RDB15125) 		Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins.
Nagai, T. et al., Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PubMed PMID 15247428.
Venus pdsVenus (RDB15994) (destabilized FP) Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells.
Ando, H. et al., Sci. Rep. 7 (1): 12642 (2017). PubMed PMID 28974737.

Cy11.5

Protein Resource (cat.num.) Reference
Cy11.5 Cy11.5/pRSETB (RDB15706) Concatenation of cyan and yellow fluorescent proteins for efficient resonance energy transfer.
Shimozono, S. et al., Biochemistry 45 (20): 6267-6271 (2006). PubMed PMID 16700538.

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Orange

Kusabira Orange

橙色の蛍光を発し、pH安定性を示します。蛍光スペクトルがシャープであるため、通常組み合わせ難い赤色蛍光タンパク質とのマルチカラー蛍光イメージングが可能です。Keima570、Keima-Red: 前者は橙色(蛍光極大570 nm)、後者は赤色(蛍光極大620 nm)の蛍光を発します。共にストークシフト (励起ピークと蛍光ピークの差) が大きく、蛍光観察時に他色の影響を受けにくいため、マルチカラー蛍光イメージングに適しています。さらにレーザーなどの単波長励起による多波長測定にも適しています。

Kusabira-Orange CoralHue™ Fluorescent Protein
Karasawa S et al. Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem J. 381, 307-312 (2004) (PMID: 15065984)
Abbr. Form Excitation maxima (nm) Emission maxima (nm) S1 vector# MC1 vector$ MN1 vector$ MCLinker vector* MNLinker vector*
KO1 Dimer 548 561 RDB18253        
mKO1 Monomer 548 559 RDB18258 RDB18259 RDB18260    
mKO2 Monomer 551 565 RDB18299        
hKO1 Dimer 548 561 RDB18254 RDB18255 RDB18256    
hmKO1 Monomer 548 559 RDB18261 RDB18262 RDB18263 RDB18264 RDB18257
hmKO2 Monomer 551 565   RDB18300 RDB18301 RDB18302 RDB18298
Protein Resource (cat.num.) Reference
Kusabira Orange hmKO1/pRSETB (RDB15312)
hmKO2/pRSETB (RDB15313) 		Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer.
Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312 (2004). PubMed PMID 15065984.
Kusabira Orange hmKO-K/pRSETB (RDB15314) Improving membrane voltage measurements using FRET with new fluorescent proteins.
Tsutsui, H. et al., Nat. Methods 5 (8): 683-685 (2008). PubMed PMID 18622396.

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Red

AzaleaB5

Protein Resource (cat.num.) Reference
AzaleaB5 AzaleaB5/pRSETb (RDB18218) Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression.
Ando, R. et al., bioRxiv (2020) doi: https://doi.org/10.1101/2020.03.30.015156.

Keima570 and Keima-Red

Emitting orange light (maximum fluorescence 570 nm, Keima570) and red light (maximum fluorescence 620 nm, Keima-Red), respectively. Both have large Stokes shift. They are suitable for multicolor fluorescence imaging, because they are hardly affected by other colors. It is also suitable for multi-wavelength measurement by single-wavelength excitation by a laser source.

Keima570 CoralHue™ Fluorescent Protein
Kogure T et al. A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nature Biotechnology. 24: 577-581 (2006) (PMID: 16648840)
Abbr. Form Excitation maxima (nm) Emission maxima (nm) S1 vector# MC1 vector$ MN1 vector$ MCLinker vector* MNLinker vector*
dKeima570 Dimer 440 570 RDB18294        
hdKeima570 Dimer 440 570 RDB18295     RDB18296 RDB18293

Keima-Red

Keima-Red CoralHue™ Fluorescent Protein
Kogure T et al. A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nature Biotechnology. 24: 577-581 (2006) (PMID: 16648840)
Abbr. Form Excitation maxima (nm) Emission maxima (nm) S1 vector# MC1 vector$ MN1 vector$ MCLinker vector* MNLinker vector*
dKeima-Red Dimer 440 616 RDB18285        
mKeima-Red Monomer 440 620 RDB18280   RDB18281    
hdKeima-Red Dimer 440 616 RDB18286     RDB18287 RDB18284
hmKeima-Red Monomer 440 620 RDB18282     RDB18283 RDB18279
Protein Resource (cat.num.) Reference
Keima-red tdKeima/pRSETB (RDB15232)
mKeima/pcDNA3 (RDB15233)
dKeima/pRSETB (RDB15234)
dKeima570/pRSETB (RDB15235) 		A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy.
Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581 (2006). PubMed PMID 16648840.
Keima-red mKeima (h)/pCS2 (RDB15236)
dKeima (h)/pCS2 (RDB15237) 		A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery.
Katayama, H. et al., Chem. Biol. 18 (8): 1042-1052 (2011). PubMed PMID 21867919.

NpF2164g5_BV4

Protein Resource (cat.num.) Reference
NpF2164g5_BV4 pCAG-EGFP-NpF2164g5_BV4 (RDB17331) Rational conversion of chromophore selectivity of cyanobacteriochromes to accept mammalian intrinsic biliverdin.
Fushimi, K., et al., Proc. Natl. Acad. Sci. U.S.A. 116 (17): 8301-8309 (2019). PubMed PMID 30948637.

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Photoconversion type

Kaede

緑色の蛍光を発します。紫外光(360 nm-410 nm)を照射することで、蛍光が赤色に不可逆に変化します。照射量に応じ、色を段階的に変えることができます。

Kaede CoralHue™ Fluorescent Protein
Ando R et al. An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc Natl Acad Sci U S A. (2002) 99(20):12651-6. (PMID: 12271129)
Abbr. Form Excitation maxima (nm) Emission maxima (nm) S1 vector# MC1 vector$ MN1 vector$ MCLinker vector* MNLinker vector*
Kaede Tetramer 508/572 518/580 RDB18241 RDB18242 RDB18243    
Protein Resource (cat.num.) Reference
Kaede Kaede/pRSETB (RDB15231) An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein.
Ando, R. et al., Proc. Natl. Acad. Sci. U.S.A. 99 (20): 12651-12656 (2002). PubMed PMID 12271129.

Kikume Green-Red

緑色の蛍光を発します。紫外光(360nm-410nm)を照射することで、蛍光が赤色に不可逆に変化します。Kaedeより高いフォトコンバージョン効率(紫外光による波長が変換する効率）を示します。

Kikume Green-Red CoralHue™ Fluorescent Protein
Habuchi S et al. mKikGR, A monomeric photoswitchable fluorescent protein. PLoS ONE 3, e3944 (2008) (PMID: 19079591)
Tsutsui H. et al. Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6, 233-238 (2005) (PMID: 15731765)
Abbr. Form Excitation maxima (nm) Emission maxima (nm) S1 vector# MC1 vector$ MN1 vector$ MCLinker vector* MNLinker vector*
KikGR1 Tetramer 507/583 517/593 RDB18272 RDB18273 RDB18274    
mKikGR1 Monomer 505/580 517/591 RDB18304        
hKikGR1 Tetramer 507/583 517/593 RDB18275 RDB18276 RDB18277 RDB18278 RDB18271
hmKikGR1 Monomer 505/580 517/591       RDB18305 RDB18303
Protein Resource (cat.num.) Reference
Kikume Green-Red KikGR/pRSETB (RDB15259) Semi-rational engineering of a coral fluorescent protein into an efficient highlighter.
Tsutsui, H. et al., EMBO Rep. 6 (3): 233-238 (2005). PubMed PMID 15731765.
Kikume Green-Red mKikGR/pRSETB (RDB15261) mKikGR, a monomeric photoswitchable fluorescent protein.
Habuchi, S. et al., PLoS One 3 (12): e3944 (2008). PubMed PMID 19079591.
h41pkve6 (derivative of Kikume Green-Red) Xpa (RDB15264)
Xpa H62Q (RDB15265) 		A diffraction-quality protein crystal processed as an autophagic cargo.
Tsutsui, H. et al., Mol. Cell 58 (1): 186-193 (2015). PubMed PMID 25773597.

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Photochromism type

Dronpa Green

緑色の蛍光を発します。蛍光の明暗が可逆的に操作でき、503 nm付近の強い光をあてると蛍光が消え、390 nm付近の光をあてると蛍光が回復するフォトクロミック蛍光タンパク質です。Photoactivated localization microscopy (PALM)法による超解像顕微鏡観察に使用できます。

Dronpa Green CoralHue™ Fluorescent Protein
Ando R et al. Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306, 1370-1373 (2004) (PMID: 15550670)
Abbr. Form Excitation maxima (nm) Emission maxima (nm) S1 vector# MC1 vector$ MN1 vector$ MCLinker vector* MNLinker vector*
DG1 Monomer 503 518 RDB18267 RDB18268 RDB18269    
hDG1 Monomer 503 518       RDB18270 RDB18266
DG3 Monomer 491 514 RDB18297        
Protein Resource (cat.num.) Reference
Dronpa-Green Dronpa/pRSETB (RDB15256)
22G/pRSETB (RDB15255) 		Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting.
Ando, R. et al., Science 306 (5700): 1370-1373 (2004). PubMed PMID 15550670.
Dronpa-Green Dronpa2/pRSETB (RDB15257)
Dronpa3/pRSETB (RDB15258) 		Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants.
Ando, R. et al., Biophys. J. 92 (12): L97-99 (2007). PubMed PMID 17384059.

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Timer type

Kusabira Green-Orange

Protein Resource (cat.num.) Reference
Kusabira Green Orange mK-GO (RDB15244) Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein.
Tsuboi, T. et al., Mol. Biol. Cell. 21 (1): 87-94 (2010). PubMed PMID 19889833.

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Non-fluorescent chromoprotein

cjBlue

Protein Resource (cat.num.) Reference
cjBlue cjBlue/pRSETB (RDB15242)
cjBlue Y64L/pRSETB (RDB15243) 		Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus.
Chan, M.C. et al., J. Biol. Chem. 281 (49): 37813-37819 (2006). PubMed PMID 17028187.

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耐熱性GFP

  • ウミサボテン由来の高い熱安定性を有する GFPです。100℃、10 分の加熱処理後でも充分な蛍光を有しています。
    参考文献. 熱安定性を有する蛍光タンパク質およびそれを利用する方法　特開2014-60947

      25℃ 70℃ 100℃
    A. victoria由来GFP 1072 1087 629
    WT 4770 4229 703
    Th1wt 8067 7236 2162
    Th2wt 7668 7525 3711
    上段、加熱処理前。下段、100℃ 10分加熱後。
    それぞれ室温で観察。     		図の蛍光強度を数値化したもの。

    (注) 画像、ならびにデータは、オリンパス株式会社から提供を受け、編集して掲載。
     

    Catalog no. Name of clone Characteristic
    RDB14364 pCoGFP-Th1wt wild type
    RDB14366 pCoGFP-Th2wt wild type
    RDB14365 pCoGFP-Th1co Optimize codons for transfection into mammalian cells
    RDB14367 pCoGFP-Th2co Optimize codons for transfection into mammalian cells

 

pH感受性GFP

pH に応じて青色 (456nm) から緑色 (507nm) に変化する蛍光を有する ウミサボテン由来のGFP です。哺乳動物細胞内における pH センサーとして活用できます。
Ogoh, K. et al. Dual-color-emitting green fluorescent protein from the sea cactus Cavernularia obesa and its use as a pH indicator for fluorescence microscopy. Luminescence 28 (4): 582-591, 2013.

362nm で励起した各種 pH 感受性 GFP の pH4 – 11 における青色から緑色への蛍光推移をイメージしたイラスト。詳しくは、Ogoh, K. et al., 2013 の Figure 6 をご覧ください。
Catalog no. Name of clone Characteristic misc.
RDB14368 pCoGFP-wt wild type GFP cDNA is cloned
in pUC19 vector.
RDB14369 pCoGFP-mam From blue to green at pH 5 – 6
RDB14370 pCoGFP-V0 From blue to green at pH 5 – 6
RDB14371 pCoGFP-V1 From blue to green at pH 6 – 7
RDB14372 pCoGFP-V2 From blue to green at pH 7 – 8
RDB14373 pCoGFP-V3 From blue to green at pH 9 – 10
RDB14374 pCoGFP-V4 From blue to green at pH 9 – 10

(注) 画像、ならびにデータは、オリンパス株式会社から提供を受け、編集して掲載。
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References

Sensor & Visualization

  • Sensor & Visualization
    • Bilirubin indicator UnaG, BReleaCa
    • cAMP indicator
    • Caspase activity indicator
    • pH sensor
    • Protein concentration in cells
    • RA indicator
    • Voltage indicator
  • Autophagy indicator
    • Chaperone mediated autophagy activity by the GAPDH-HT indicator by Dr. Takahiro Seki’s lab as well as fluorescent protein probes of LC3B accumulation developed by Dr. Noboru Mizushima’s lab, Dr. Itaru Hamachi’s lab and Dr. Keiji Kimura’s lab are available.
  • Calcium-ion sensor
    • Fluorescent protein-based Ca2+ sensors, G-CaMPs, Yellow Cameleons and Pericams developed by Dr. Jin-ichi Nakai’s lab and Dr. Atsushi Miyawaki’s lab, which are composed with calmodulin, fluorescent protein and M13 peptide (CaM binding domain of myosin light chain kinase), are designated to visualize intracellular [Ca2+] dynamics.
    • G-CaMP
    • Pericam
    • Yellow Cameleon
  • Cell cycle indicator Fucci
    • To monitor cell cycle progression in living cells, cell cycle indicator Fucci probes deveoped by Dr. Atsushi Miyawaki’s lab are available.
  • Epigenetics reporter
    • Visualization of histone acetylation: The Histac fluorescent probes deposited by Dr. Kazuki Sasaki allow you monitoring the state of activity of acetylation of histone H4 by fluorescence in living cells.
    • Visualization of methylated DNA: The EGFP-MBD-nls protein recognizes the methylated DNA and you can follow status of the DNA methylation in situ under physiological conditions using the pEGFP-MBD-nls expression clone.
  • Knock in markers with CRISPR/Cas9 genome editing
    • As a part of Auxin Inducible Degron (AID) System clones, Dr. Masato Kanemaki provides a series of knock-in fluorescent and selection markers with CRISPR/Cas9 genome editing.
  • Notch signaling reporter
    • The pRBS-EGFP and RBP-J-Venus expression clones deposited by Dr. Makoto Mark Taketo and Dr. Kenji Tanigaki, respectively, allow you monitoring the state of activation of the Notch signaling by fluorescence in living cells.
  • Organelle marker/subcellular localization
  • Sphingolipid marker
    • Lipid rafts are small lipid domains on the cell membrane and are thought to play an important role in signal transduction, endocytosis and more. We provide fluorescent probes for sphingomyelin and cholesterol lipid domains.
    • Nakanori: sphingomyelin and cholesterol lipid domain (lipid raft)
    • D4 toxin: cholesterol rich domain
    • lysenin: sphingomyelin

Luminescent protein

  • Nano-lantern luminescent and fluorescent protein
    • Nano lantern (NL) consists of Renilla luciferase and adjacent fluorescence protein. The chemiluminescence of the luciferase provides light source for excitation and enables the fluorescence protein to be observed. Three colors of NLs, yellow, cyan and orange, have been developed. NLs do not require external light source and overcome problems such as autofluorescence, phototoxicity, and photobleaching.
  • Higher intensity luciferases having Green-, Yellow- or Red-emission by using D-luciferin
    • These luciferases have at most four-time maximum luminous intensity than that of widely used luciferase of the Photinus pyralis, a common North American firefly. Further more, Green-, Yellow- or Red-emission can be obtained by using D-luciferin as a substrate of each luciferase.
  • AkaLuc luciferase providing brighter and red-shifted luminescence
    • The artificial bioluminescence system AkaBLI enables noninvasive signal observation in deep tissue of living animals. It was developed by Dr. Atsushi Miyawaki and Dr. Satoshi Iwano of the RIKEN Center for Brain Science, and Dr. Shojiro Maki of the University of Electro-Communications. The AkaBLI consists of an artificial substrate AkaLumine with improved tissue permeability and an artificial luciferase Akaluc optimized to AkaLumine. The intensity of the luminescence of AkaBLI system is 100 to 1000 folds brighter than the conventional systems.

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(GRP0050j 2016.11.16 N.N.)

2021.03.28



 

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