Biosensor & Indicator
Autophagy
- Chaperone mediated autophagy activity by the GAPDH-HT indicator by Dr. Takahiro Seki’s lab as well as fluorescent protein probes of LC3B accumulation developed by Dr. Noboru Mizushima’s lab, Dr. Itaru Hamachi’s lab and Dr. Keiji Kimura’s lab are available.
Mitophagy
- Dr. Miyawaki Atsushi and Dr. Katayama Hiroyuki of RIKEN Center for Brain Science (CBS) and Dr. Hioki Hiroyuki of Juntendo University and their colleagues have recently developed mito-SRAI, a fluorescent sensor that can quantitatively visualize mitophagy both in live and fixed conditions.
Fucci cell cycle indicator
- To monitor cell cycle progression in living cells, cell cycle indicator Fucci probes deveoped by Dr. Atsushi Miyawaki’s lab are available.
Calcium-ion
- Fluorescent protein-based Ca2+ sensors, G-CaMPs, Yellow Cameleons and Pericams developed by Dr. Jin-ichi Nakai’s lab and Dr. Atsushi Miyawaki’s lab, which are composed with calmodulin, fluorescent protein and M13 peptide (CaM binding domain of myosin light chain kinase), are designated to visualize intracellular [Ca2+] dynamics.
- G-CaMP
- Pericam
- Yellow Cameleon
Sphingolipid
- Lipid rafts are small lipid domains on the cell membrane and are thought to play an important role in signal transduction, endocytosis and more. We provide fluorescent probes for sphingomyelin and cholesterol lipid domains.
- Nakanori: sphingomyelin and cholesterol lipid domain (lipid raft)
- D4 toxin: cholesterol rich domain
- lysenin: sphingomyelin
Organelle marker
- We are providing genetic resources for visualization of organelles such as mitochondria and nucleus. Each clone contains an organelle localization signal sequence fused with fluorescent proteins or epitope tags. Organelles can be detected by fluorescence or by detecting epitope tags with antibodies.
Inter-organelle contact sites
- Divided GFP-fragment have a feature that they emit fluorescence again by reconstitution when each fragment is in close proximity. When labelled organelles such as mitochondria and ERs by the divided GFPs exist together, the contact sites in cells are visualized by the reconstituted fluorescent protein. The vectors of fluorescent protein probes developed by Dr. Yasushi Tamura of the Yamagata University, Dr. Toshiya Endo of the Kyoto Sangyo University and their colleague are available from the DNA Bank.
- Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system.
Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y.
Sci. Rep. 8 (1): 6175 (2018). PubMed PMID 29670150.
FPs with tissue specific promoter
ATP imaging
cAMP imaging
pH sensor
Epigenetics
Sensor misc.
2025.05.03 (T.M. GRP0058e)
