Resarch Tools of Mitophagy
A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag.
Yim, W.W., Yamamoto, H., Mizushima, N.
Elife 11: e78923 (2022). PubMed PMID 35938926.
| Catalog no. | Name of resource | Description |
| RDB19962 | pMRX-IB-pSu9-HaloTag7-mGFP | Retroviral vector for stable expression of pSu9-HaloTag7-mGFP to measure mitophagy activity. |
| RDB19963 | pMRX-IB-HaloTag7-mGFP-KDEL | Retroviral vector for stable expression of HaloTag7-mGFP-KDEL to measure ER-phagy activity. |
Visualizing and Modulating Mitophagy for Therapeutic Studies of Neurodegeneration.
Katayama, H., Hama, H., Nagasawa, K., Kurokawa, H., Sugiyama, M., Ando, R., Funata, M., Yoshida, N., Homma, M., Nishimura, T., Takahashi, M., Ishida, Y., Hioki, H., Tsujihata, Y., Miyawaki, A. Cell 181 (5): 1176-1187.e16 (2020). PubMed PMID 32437660.
| Catalog no. | Name of resource | Description |
| RDB18222 | SRAI_pcDNA3 | Expression vector of fluorescent protein, SRAI (signal-retaining autophagy indicator) for autophagy sensor. |
| RDB18223 | mito-SRAI_pcDNA3 | Expression vector of fluorescent protein, mito-SRAI (signal-retaining autophagy indicator) for quantitative visualizing mitophagy. |
| RDB18684 | pAAV2-TRE-mito-SRAI-BGHpA | AAV2 expression vector of mito-SRAI (signal-retaining autophagy indicator) driven by the tetracyclin-responsive element promoter. |
Glycolytic suppression dramatically changes the intracellular metabolic profile of multiple cancer cell lines in a mitochondrial metabolism-dependent manner.
Shiratori, R., Furuichi, K., Yamaguchi, M., Miyazaki, N., Aoki, H., Chibana, H., Ito, K., Aoki, S. Sci. Rep. 9 (1): 18699 (2019). PubMed PMID 31822748.
| Catalog no. | Name of resource | Description |
| RDB18369 | mitoKeima-Red | Expression vector encoding mitochondrially targeted Keima-Red (mitoKeima-Red). |
A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery.
Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., Miyawaki, A.
Chem. Biol. 18 (8): 1042-1052 (2011). PubMed PMID 21867919.
| Catalog no. | Name of resource | Description |
| RDB15236 | mKeima (h)/pCS2 | Expression vector of monomeric Keima-Red (mKeima). |
| RDB15237 | dKeima (h)/pCS2 | Expression vector of dimeric Keima-Red (dKeima). |
| RDB15238 | mt-mKeima (h)/pIND(SP1) | Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal. |
| RDB15456 | mt-mKeima/pcDNA3 | Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal. |
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