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Biosensor misc.

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Biosensor & Indicator

Curated topics

Bilirubin indicator UnaG, BReleaCa

  • A dual-ligand-modulable fluorescent protein based on UnaG and calmodulin.
    Shitashima, Y., Shimozawa, T., Asahi, T., Miyawaki, A.
    Biochem. Biophys. Res. Commun. 496 (3): 872-879 (2018). PubMed PMID 29395087.

    Catalog no. Name of resource Descriptiion
    RDB15908 pRSETB-BReleaCa Bacteria expression vector of UnaG/CaM hybrid protein, BReleaCa, capable of binding to bilirubin and calcium.
    Catalog no. Name of resource Descriptiion
    RDB15909 pcDNA3-BReleaCa Expression vector of UnaG/CaM hybrid protein, BReleaCa, capable of binding to bilirubin and calcium.
  • Continuous de novo biosynthesis of haem and its rapid turnover to bilirubin are necessary for cytoprotection against cell damage.
    Takeda, T.A., Mu, A., Tai, T.T., Kitajima, S., Taketani, S.
    Sci. Rep. 5: 10488 (2015). PubMed PMID 25990790.

    Catalog no. Name of resource Descriptiion
    RDB13504 pET-His UNAG Expression clone of eel UnaG in E.coli.
  • A bilirubin-inducible fluorescent protein from eel muscle.
    Kumagai, A., Ando, R., Miyatake, H., Greimel, P., Kobayashi, T., Hirabayashi, Y., Shimogori, T., Miyawaki, A.
    Cell 153 (7): 1602-1611(2013). PubMed PMID 23768684.

    Catalog no. Name of resource Descriptiion
    RDB15703 UnaG/pcDNA3-FLAG Expression vector of eel UnaG, bilirubin-inducible fluorescent protein.
    Catalog no. Name of resource Descriptiion
    RDB15704 UnaG/pGEX-2T xpression vector of eel UnaG, bilirubin-inducible fluorescent protein.

Caspase activity indicator

  • A high-throughput method for development of FRET-based indicators for proteolysis.
    Nagai, T., Miyawaki, A.
    Biochem. Biophys. Res. Commun. 319 (1): 72-77 (2004). PubMed PMID 15158444.

    Catalog no. Name of resource Descriptiion
    RDB15705 SCAT3.1/pcDNA4 HMB Expression vector of FRET based apoptosis probe.
  • Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer.
    Karasawa, S., Araki, T., Nagai, T., Mizuno, H., Miyawaki, A.
    Biochem. J. 381 (Pt 1): 307-312 (2004). PubMed PMID 15065984.

    Catalog no. Name of resource Descriptiion
    RDB15240 MiCy-DEVD-mKO1/pCS2 Expression vector of FRET based caspase3 activity monitoring probe.

Concentration of Protein in cells

  • Dependence of fluorescent protein brightness on protein concentration in solution and enhancement of it.
    Morikawa, J.T., Fujita, H., Kitamura, A., Horio, T., Yamamoto, J., Kinjo, M., Sasaki, A., Machiyama, H., Yoshizawa, K., Ichimura, T., Imada, K., Nagai, T., Watanabe, M.T.
    Sci. Rep. 6: 22342, 2016. PubMed PMID: 26956628

    Catalog no. Name of resource Descriptiion
    RDB14203 pPAL7_GimRET (CFP-YFP1G/pPAL7) Expression vector of GimRET (Glycine inserted mutant FRET probe) for E. coli.
    RDB14204 pGimRET (CFP-YFP1G/pECFP) Expression vector of GimRET (Glycine inserted mutant FRET probe) for mammalian cells.

RA indicator

  • Visualization of an endogenous retinoic acid gradient across embryonic development.
    Shimozono, S., Iimura, T., Kitaguchi, T., Higashijima, S., Miyawaki, A.
    Nature 496 (7445): 363-366 (2013). PubMed PMID 23563268.

    Catalog no. Name of resource Descriptiion
    RDB15249 GEPRA-B Genetically encoded indicator for RA, GEPRA, having ligand binding domain from the RAR-beta.
    RDB15250 GEPRA-G Genetically encoded indicator for RA, GEPRA, having ligand binding domain from the RAR-gamma.
    RDB15251 GEPRA-AA Genetically encoded indicator for RA, GEPRA. Low-affinity verson of the GEPRA-B.
    RDB15252 GEPRA-B/ pT2KXIGdeltain Expression vector of genetically encoded indicator for RA, GEPRA. For generation of transgenic zebrafish.
    RDB15253 GEPRA-G/ pT2KXIGdeltain Expression vector of genetically encoded indicator for RA, GEPRA. For generation of transgenic zebrafish.
    RDB15254 GEPRA-AA/ pT2KXIGdeltain Expression vector of genetically encoded indicator for RA, GEPRA. For generation of transgenic zebrafish. Low-affinity verson of the GEPRA-B.

Rab11: active Rab11 visualization

  • RBD11, a bioengineered Rab11-binding module for visualizing and analyzing endogenous Rab11.
    Osaki F, Matsui T, Hiragi S, Homma Y, Fukuda M.
    J. Cell Sci. 134 (7): jcs257311 (2021). PubMed PMID 33712449.

    Catalog no. Name of resource Descriptiion
    RDB18788 pMRX-puro-EGFP-RBD11 Retroviral expression vector for visualization of active Rab11: mouse Rab11-specific binding domain (RBD11) tagged with EGFP.
    RDB18789 pMRX-puro-EGFP-RBD11-mut Retroviral expression vector of negative control for visualization of active Rab11: mouse Rab11-specific binding domain (RBD11) mutant tagged with EGFP.
    RDB18790 pMRX-puro-EGFP-2xRBD11 Retroviral expression vector of negative control for visualization of active Rab11: mouse Rab11-specific binding domain (RBD11) mutant tagged with EGFP.
    RDB18791 pMRX-puro-EGFP-2xRBD11-mut Retroviral expression vector of negative control for visualization of active Rab11: mouse Rab11-specific binding domain (RBD11) mutant tagged with EGFP.
    RDB18792 pMRX-puro-EGFP-FM-RBD11 Retroviral expression vector of active Rab11 trapper: FM-tagged mouse Rab11-specific binding domain (RBD 11) tagged with EGFP.
    RDB18793 pMRX-puro-EGFP-FM-RBD11-mut Retroviral expression vector of negative control of active Rab11 trapper: FM-tagged mouse Rab11-specific binding domain (RBD 11) mutant tagged with EGFP.

Temperature indicator

  • A highly-sensitive genetically encoded temperature indicator exploiting a temperature-responsive elastin-like polypeptide.
    Vu, C.Q., Fukushima, S.I., Wazawa, T., Nagai, T.
    Sci. Rep. 11 (1): 16519 (2021). PubMed PMID 34389773.

    Catalog no. Name of resource Descriptiion
    RDB19861 ELP-TEMP/pcDNA3.1(-) Vector of ELP-TEMP, a genetically encoded highly-sensitive temperature indicator, for mammalian expression.
  • Genetically encoded ratiometric fluorescent thermometer with wide range and rapid response.
    Nakano, M., Arai, Y., Kotera, I., Okabe, K., Kamei, Y., Nagai, T.
    PLoS One 12 (2): e0172344 (2017). PubMed PMID 28212432.

    Catalog no. Name of resource Descriptiion
    RDB19869 gTEMP/pcDNA3 Vector of gTEMP, a genetically-encoded temperature indicator, for mammalian expression.

Voltage indicator

  • Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging.
    Inagaki, S., Tsutsui, H., Suzuki, K., Agetsuma, M., Arai, Y., Jinno, Y., Bai, G., Daniels, M.J., Okamura, Y., Matsuda, T., Nagai, T.
    Sci. Rep. 7: 42398 (2017). PubMed PMID 28205521.

    Catalog no. Name of resource Descriptiion
    RDB19881 LOTUS-V/pcDNA3 Vector of LOTUS-V, a fluorescent protein, for mammalian expression.
  • Improving membrane voltage measurements using FRET with new fluorescent proteins.
    Tsutsui, H., Karasawa, S., Okamura, Y., Miyawaki, A.
    Nat. Methods 5 (8): 683-685 (2008). PubMed PMID 18622396.

    Catalog no. Name of resource Descriptiion
    RDB15262 Mermaid Voltage indicator Mermaid.
  • Improved detection of electrical activity with a voltage probe based on a voltage-sensing phosphatase.
    Tsutsui, H., Jinno, Y., Tomita, A., Niino, Y., Yamada, Y., Mikoshiba, K., Miyawaki, A., Okamura, Y.
    J. Physiol. 591 (18): 4427-4437 (2013). PubMed PMID 23836686.

    Catalog no. Name of resource Descriptiion
    RDB15263 Mermaid2 Voltage probe Mermaid2.

2025.05.03 (T.M.)



 

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