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Calcium Ion Sensor Clones


Calcium-ion sensor clones

Fluorescent protein-based Ca2+ sensors, which are composed with calmodulin, fluorescent protein and M13 peptide (CaM binding domain of myosin light chain kinase), are designated to visualize intracellular [Ca2+] dynamics.
Various improvements of the Ca2+ sensors have been made for optimum temperature, signal/noise ratio, susceptibility and so on.
Many papers using Ca2+ sensors have been published reporting the estimation of calcium concentration.

G-CaMP and R-CaMP
probe name Ca2+affinity (Kd, nM) dynamic range (Fmax/Fmin) features&application refference plasmid clone
(cat. num)
G-CaMP1.6 146 4.9 Brightness improved version. Work at 25oC (hippocampal slice of rat) to 28oC (HEK293 cell, HeLa cell) but not at 37oC (HEK293 cell). PMID 11175727, 16159115, 16537386 pN1-G-Camp1.6 (RDB06749)
G-CaMP2 146 3.9 to 4.9 Heat stable version. Work at 37oC (HEK293 cell, heart of mouse embryo, primary culture of hippocampal astrocytes). PMID 16537386, 22472649 pN1-G-CaMP2 (RDB06750)
G-CaMP-HS 102 4.1 High-Ca2+ affinity version (ie: mammalian cell, zebrafish). PMID 21383146, 27239939 G-CaMP-HS (RDB14607)
G-CaMP6 158 11.4 to 27.9 Sensitivity improved version. Quick ON/OFF response allow to observe neuronal-cell calcium spikes (ie: mouse brain section, mouse B cell, C. elegans). PMID 23240011, 27385517, 27428827 G-CaMP6 (RDB14609)
pMXs-IRES-hNGFR_G-CaMP6 (RDB14513)
G-CaMP7.09 212 19.3 Enhanced brightness allow to observe flashing fluorescence of iPS cell-derived cardiomyocytes of cynomolgus monkey. PMID 27723741 G-CaMP7.09 (RDB14612)
R-CaMP1.07 192 14.4 to 28.7 CaMP with a red fluorescent protein. PMID 22808076, 25419959 R-CaMP1.07 (RDB14608)
Yellow Cameleon
probe name Ca2+affinity (Kd, nM) dynamic range (Fmax/Fmin) features&application refference plasmid clone
(cat. num)
YC2.12 Yellow cameleon     Ca2+ indicator based on calmodulin and FRET by CFP-Venus pair (ie. Purkinje neuron of mouse cerebellar slice). PMID 11753368, 15247428 YC2.12/pCS2 (RDB15132)
YC3.12 Yellow cameleon 1,500   Low-affinity Ca2+ indicator based on calmodulin and FRET by CFP-Venus pair. PMID 15247428 YC3.12/pCS2 (RDB15133)
YC2.60 Yellow cameleon 40 5.6 Ca2+ indicator based on calmodulin and FRET by CFP-Venus pair. PMID 15247428 YC2.60/pRSETB (RDB15134)
YC3.60 Yellow cameleon 250 5.6 Low-affinity Ca2+ indicator based on calmodulin and FRET by CFP-Venus pair (ie. HeLa cells and hippocampal brain slice of transgenic mouse). PMID 15247428 YC3.60/pcDNA3 (15135)
YC3.60/pcDNA3 (15136)
YC3.60pm/pcDNA3 (15137)
YC4.60 Yellow cameleon 58 3.6 Low-affinity Ca2+ indicator based on calmodulin and FRET by CFP-Venus pair. PMID 15247428 YC4.60/ pRSETB (RDB15138)
Pericam
probe name Ca2+affinity (Kd, nM) dynamic range (Fmax/Fmin) features&application refference plasmid clone
(cat. num)
Flash-pericam 700   Designed to work as a single wavelength indicator of intracellular Ca2+. Better at 28-30oC than 37oC. (HeLa cells) PMID 11248055 Flash pericam/pcDNA3 (RDB15139)
Ratiometric-pericam 1,700 10 Heat stable version. Work at 37oC. (HeLa cells) PMID 11248055 Ratiometric pericam/pcDNA3 (RDB15143)
Ratiometric pericam-mt/pcDNA3 (RDB15144)
Ratiometric pericam-nu/pcDNA3 (RDB15145)
Inverse pericam 200   Becoming dimmed with Ca2+. (HeLa cells) PMID 11248055 Inverse pericam/pcDNA3 (RDB15140)

G-CaMP and R-CaMP clones (Dr. Jin-ichi Nakai, Saitama University)

Catalog # Name of clone Publication
RDB06747 pN1-G-CaMP Nakai, J., A high signal-to-noise Ca2+ probe composed of a single green fluorescent protein. Nat. Biotechnol., 19, 137-141 (2001). PMID 11175727.
RDB06748 pN1-G-CaMP1.3 Nakai, J., A high signal-to-noise Ca2+ probe composed of a single green fluorescent protein. Nat. Biotechnol., 19, 137-141 (2001). PMID 11175727.
RDB06749 pN1-G-CaMP1.6 Ohkura, M., Genetically encoded bright Ca2+ probe applicable for dynamic Ca2+ imaging of dendritic spines. Anal. Chem., 77, 5861-5869 (2005). PMID 16159115.
RDB06750 pN1-G-CaMP2 Tallini, Y.N., Imaging cellular signals in the heart in vivo: Cardiac expression of the high-signal Ca2+indicator GCaMP2. Proc. Natl. Acad. Sci. U S A., 103, 4753-4758 (2006). PMID 16537386.
RDB14606 G-CaMP4.1 Shindo, A., Tissue-tissue interaction-triggered calcium elevation is required for cell polarization during Xenopus gastrulation. PLoS One 5 (2): e8897 (2010). PMID 20126393.
RDB14607 G-CaMP-HS Muto, A., Genetic visualization with an improved GCaMP calcium indicator reveals spatiotemporal activation of the spinal motor neurons in zebrafish. Proc. Natl. Acad. Sci. U.S.A. 108 (13): 5425-5430 (2011). PMID 21383146.
RDB14608 R-CaMP1.07 Ohkura, M., An improved genetically encoded red fluorescent Ca2+ indicator for detecting optically evoked action potentials. PLoS One 7 (7): e39933 (2012). PMID 22808076.
RDB14609 G-CaMP6 Ohkura, M., Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. PLoS One 7 (12): e51286 (2012). PMID 23240011.
RDB14610 G-CaMP7 Ohkura, M., Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. PLoS One 7 (12): e51286 (2012). PMID 23240011.
RDB14611 G-CaMP8 Ohkura, M., Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. PLoS One 7 (12): e51286 (2012). PMID 23240011.
RDB14612 G-CaMP7.09 Shiba, Y., Allogeneic transplantation of iPS cell-derived cardiomyocytes regenerates primate hearts. Nature 538 (7625): 388-391 (2016). PMID 27723741.

Related Articles

  • Arizono, M., Bannai, H., Nakamura, K., Niwa, F., Enomoto, M., Matsu-ura, T., Miyamoto, A., Sherwood, M.W., Nakamura, T., Mikoshiba, K. Receptor-Selective Diffusion Barrier Enhances Sensitivity of Astrocytic Processes to Metabotropic Glutamate Receptor Stimulation. Science Signaling, 2012. doi: 10.1126/scisignal.2002498
  • Press Release (2012, in Japanese)
  • Press Release (2008, in Japanese)

Yellow Cameleon clones (Dr. Atsushi Miyawaki, RIKEN BSI)

Catalog # Name of clone Publication
RDB15132 YC2.12/pCS2 Nagai, T., A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20 (1): 87-90 (2002). PMID 11753368. (original).
RDB15134 YC2.60/pRSETB Nagai, T., Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428. (original).
RDB15455 YCX2.60/pCS2 Madisen, L., Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron 85 (5): 942-958 (2015). PMID 25741722. (original).
RDB15133 YC3.12/pCS2 Nagai, T., Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428. (original).
RDB15135 YC3.60/pcDNA3 Nagai, T., Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428. (original).
RDB15136 YC3.60/pRSETB Nagai, T., Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428. (original).
RDB15137 YC3.60pm/pcDNA3 Nagai, T., Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428. (original).
RDB15138 YC4.60/pRSETB Nagai, T., Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PMID 15247428. (original).

Related Articles


Pericam clones (Dr. Atsushi Miyawaki, RIKEN BSI)

Catalog # Name of clone Publication
RDB15139 Flash pericam/pcDNA3 Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055. (original).
RDB15140 Inverse pericam/pcDNA3 Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055. (original).
RDB15141 Split pericam-N/pcDNA3 Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055. (original).
RDB15142 Split pericam-C/pcDNA3 Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055. (original).
RDB15143 Ratiometric pericam/pcDNA3 Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055. (original).
RDB15144 Ratiometric pericam-mt/pcDNA3 Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055. (original).
RDB15145 Ratiometric pericam-nu/pcDNA3 Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055. (original).

Related Articles


(GRP0029e 2012.04.06 T.M. )

2018.07.26