Clone Search

Resources for

Clontech Laboratories, Inc. and RIKEN BioResource Research Center concluded license agreement on preservation and distribution of Fluorescent Proteins, DsRed2 and mCherry for academic use

Clones & Vectors for Gene Expression
  Promoter collection
  Epitope tag fusion clones
  SEREX cDNA clone
  HLA antigen cDNA clone
  Nakamura & White RFLP clone

Resources for Gene Analysis
  Cultured microbe
  Cultured animal cell
  Experimental animal
  in vitro experiment
  Plant gene resources

Clone Set & Library
  Genomic clone
  cDNA clone
  Expression clone

Recombinant Virus
  Recombinant Adenovirus
  Shuttle Vector for Recombinant Virus

Genomic DNA
  JCM Microbial Genomic DNA
  BRC Mouse Genomic DNA

Search for resources
  Key word search
  Depositors List
  Gene Set Collection

BRC News

Resources for Gene Analysis

Genome Editing

  • Genome Editing by CRISPR/Cas9
    • Expression vector of Cas9 enzyme
    • Plasmids for the evaluation of the efficiency of genome editing
    • Knock-in markers with CRISPR/Cas9 genome editing

Luminescent protein

  • Nano-lantern luminescent and fluorescent protein
    • Nano lantern (NL) consists of Renilla luciferase and adjacent fluorescence protein. The chemiluminescence of the luciferase provides light source for excitation and enables the fluorescence protein to be observed. Three colors of NLs, yellow, cyan and orange, have been developed. NLs do not require external light source and overcome problems such as autofluorescence, phototoxicity, and photobleaching.
  • Higher intensity luciferases having Green-, Yellow- or Red-emission by using D-luciferin
    • These luciferases have at most four-time maximum luminous intensity than that of widely used luciferase of the Photinus pyralis, a common North American firefly. Further more, Green-, Yellow- or Red-emission can be obtained by using D-luciferin as a substrate of each luciferase.
  • AkaLuc luciferase providing brighter and red-shifted luminescence
    • The artificial bioluminescence system AkaBLI enables noninvasive signal observation in deep tissue of living animals. It was developed by Dr. Atsushi Miyawaki and Dr. Satoshi Iwano of the RIKEN Center for Brain Science, and Dr. Shojiro Maki of the University of Electro-Communications. The AkaBLI consists of an artificial substrate AkaLumine with improved tissue permeability and an artificial luciferase Akaluc optimized to AkaLumine. The intensity of the luminescence of AkaBLI system is 100 to 1000 folds brighter than the conventional systems.

Visualization (FP research tool)

  • Autophagy indicator
    • Chaperone mediated autophagy activity by the GAPDH-HT indicator by Dr. Takahiro Seki’s lab as well as fluorescent protein probes of LC3B accumulation developed by Dr. Noboru Mizushima’s lab, Dr. Itaru Hamachi’s lab and Dr. Keiji Kimura’s lab are available.
  • Calcium-ion sensor
    • Fluorescent protein-based Ca2+ sensors, G-CaMPs, Yellow Cameleons and Pericams developed by Dr. Jin-ichi Nakai’s lab and Dr. Atsushi Miyawaki’s lab, which are composed with calmodulin, fluorescent protein and M13 peptide (CaM binding domain of myosin light chain kinase), are designated to visualize intracellular [Ca2+] dynamics.
    • G-CaMP
    • Pericam
    • Yellow Cameleon
  • Protein concentration in cells
    • GimRET
  • pH sensor
    • Sea cactus GFP: Dual-color-emitting green fluorescent protein used as pH indicators from the sea cactus Cavernularia obesa developed by Dr. Katsunori Ogoh of the Olympus corporation.
  • Cell cycle indicator Fucci
    • To monitor cell cycle progression in living cells, cell cycle indicator Fucci probes deveoped by Dr. Atsushi Miyawaki’s lab are available.
  • Notch signaling reporter
    • The pRBS-EGFP and RBP-J-Venus expression clones deposited by Dr. Makoto Mark Taketo and Dr. Kenji Tanigaki, respectively, allow you monitoring the state of activation of the Notch signaling by fluorescence in living cells.
  • Epigenetics reporter
    • Visualization of histone acetylation: The Histac fluorescent probes deposited by Dr. Kazuki Sasaki allow you monitoring the state of activity of acetylation of histone H4 by fluorescence in living cells.
    • Visualization of methylated DNA: The EGFP-MBD-nls protein recognizes the methylated DNA and you can follow status of the DNA methylation in situ under physiological conditions using the pEGFP-MBD-nls expression clone.
  • Organelle marker/subcellular localization
    • We are providing genetic resources for visualization of organelles such as mitochondria and nucleus. Each clone contains an organelle localization signal sequence fused with fluorescent proteins or epitope tags. Organelles can be detected by fluorescence or by detecting epitope tags with antibodies.
  • Sensor, Indicator
    • RA indicator
    • voltage indicator
    • cAMP indicator
    • bilirubin indicator UnaG, BReleaCa
    • Caspase activity indicator
  • Knock in markers with CRISPR/Cas9 genome editing
    • As a part of Auxin Inducible Degron (AID) System clones, Dr. Masato Kanemaki provides a series of knock-in fluorescent and selection markers with CRISPR/Cas9 genome editing.
  • Fluorescent protein resource
  • Further resources of Visualization (FP research tool)

Mammalian cell

E. coli

Thermus thermophilus HB8

Schizosaccharomyces pombe

Saccharomyces cerevisiae

Other resources

(2006.02.16 T.M.)