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  • The 2017 Nobel Prize in Physiology or Medicine (2017.10.03 T.M.)
    • Genetic resources for Circadian clock genes are available.
    • Looking forward to receiving your request.
      Plase visit Circadian clock genes [link].

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Stable ChIP-seq analysis using transcription factors tagged with biotinylated peptide tag. (2018/2/16 K.N.)

  • ChIP-seq is a powerful method of comprehensive analysis of transcription factor (TF) binding sites. However, the bottle neck of ChIP-seq analysis is the quality of antibody, and thus many researchers have been given up. To overcome, authors constructed the vector expressing “biotinylated-tag” transcription factors. With this vector, you can get acceptable level of TF-DNA complex for ChIP-seq analysis by streptavidin-beads for any kinds of TF.[more]

Fluorescent probes for sphingomyelin and cholesterol lipid domains.(2018/02/06 N.N.)

  • Lipid rafts are small lipid domains on the cell membrane and are thought to play an important role in signal transduction, endocytosis and more. Because of the lack of appropriate probes to label sphingomyelin and cholesterol individually, detailed analysis of lipid rafts was difficult.[more]

Hedgehog signal analysis by the Gli recognition element reporter. (2018/2/5 T.M.)

  • To elucidate roles of oxidative stress during the induction of osteoporosis and inhibition of hedgehog signaling, osteoblast differentiation in murine embryonic mesenchymal cell C3H10T1/2 was investigated with a focus on the interaction with mitogen-activated protein kinase (MAPKs) as possible mediator. To monitor Gli-mediated transcription activity in C3H10T1/2 cells under the oxidative stress and application of MAPK inhibitors, luciferase reporter having Gli-transcription factor recognition element was used and the inhibitory effect of JNK1 on Gli transcription was suggested. [more]

Genes promoting hepatocyte differentiation from human iPS cells. (2018/2/5 T.M.)

  • Expression profile of genes was compared between human adult liver and 201B7 cells (iPS cells). Sixteen genes were identified as initial candidates and these expression vectors were constructed. To identify genes promoting hepatocyte differentiation from iPS cells, 210B7 cells ware transfected with these expression vector to screen genes that upregulate alpha-feto protein (AFP) and downregulate Nanog and identified CEBPA, CEBPB, FOXA1 and FOXA3 genes.[more]

Fluorescent Ubiquitination-based Cell Cycle Indicator Fucci expression plasmid (2017/11/15 N.N.)

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Distribution of Host E. coli strains for incorporation of synthetic amino acids. (2017/07/25 N.N.)

  • The host E. coli strains, RFzero-iy strain and B-95.deltaA strain that can produce recombinant proteins incorporating synthetic amino acids by reassignment of the UAG codon.[more…]

Evaluation of chaperone mediated autophagy (CMA) activity by the GAPDH-HT indicator. (2017/06/29 N.N.)

  • “GAPDH-HT indicator” is a CMA marker consisting of HaloTag and GAPDH which is a typical substrate of CMA. The CMA activity in the cells can be evaluated by using GAPDH-HT indicator and an appropriate under the fluorescent-labeled HaloTag ligand.[more]

M-INK, a novel tool for tagging the mature melanosomes.(2017/05/02 N.N.)

  • A novel tool “M-INK” to visualize melanosome in melanocyte and keratinocyte has arrived.[more]

G-CaMP, a fluorescent calcium sensor.(2017/03/16 N.N.)

  • A Fluorescent probes “G-CaMP” for measuring intracellular calcium concentration are available.[more]


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