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Clontech Laboratories, Inc. and RIKEN BioResource Research Center concluded license agreement on preservation and distribution of Fluorescent Proteins, DsRed2 and mCherry for academic use

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Dnaconda’s Recommendationdnaconda01


AkaLuc luciferase providing brighter and red-shifted luminescence (2018/5/7 N.N.)

  • Firefly bioluminescence systems have been commonly used as a imaging tool of biological phenomena. However, it have not been strong enough for imaging signals in deep tissues, because of low permeabilization of substrates. Recently, Dr. Atsushi Miyawaki and Dr. Satoshi Iwano of the RIKEN Center for Brain Science, and Dr. Shojiro Maki of the University of Electro-Communications developed a system of artificial bioluminescence AkaBLI that enables noninvasive signal observation in deep tissue of living animals. [more]

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Stable ChIP-seq analysis using transcription factors tagged with biotinylated peptide tag. (2018/2/8 K.N.)

  • ChIP-seq is a powerful method of comprehensive analysis of transcription factor (TF) binding sites. However, the bottle neck of ChIP-seq analysis is the quality of antibody, and thus many researchers have been given up. To overcome, authors constructed the vector expressing “biotinylated-tag” transcription factors. With this vector, you can get acceptable level of TF-DNA complex for ChIP-seq analysis by streptavidin-beads for any kinds of TF.[more]

Fluorescent probes for sphingomyelin and cholesterol lipid domains.(2018/02/06 N.N.)

  • Lipid rafts are small lipid domains on the cell membrane and are thought to play an important role in signal transduction, endocytosis and more. Because of the lack of appropriate probes to label sphingomyelin and cholesterol individually, detailed analysis of lipid rafts was difficult.[more]


Hedgehog signal analysis by the Gli recognition element reporter. (2018/2/5 T.M.)

  • To elucidate roles of oxidative stress during the induction of osteoporosis and inhibition of hedgehog signaling, osteoblast differentiation in murine embryonic mesenchymal cell C3H10T1/2 was investigated with a focus on the interaction with mitogen-activated protein kinase (MAPKs) as possible mediator. To monitor Gli-mediated transcription activity in C3H10T1/2 cells under the oxidative stress and application of MAPK inhibitors, luciferase reporter having Gli-transcription factor recognition element was used and the inhibitory effect of JNK1 on Gli transcription was suggested. [more]


Genes promoting hepatocyte differentiation from human iPS cells. (2018/2/5 T.M.)

  • Expression profile of genes was compared between human adult liver and 201B7 cells (iPS cells). Sixteen genes were identified as initial candidates and these expression vectors were constructed. To identify genes promoting hepatocyte differentiation from iPS cells, 210B7 cells ware transfected with these expression vector to screen genes that upregulate alpha-feto protein (AFP) and downregulate Nanog and identified CEBPA, CEBPB, FOXA1 and FOXA3 genes.[more]

Fluorescent Ubiquitination-based Cell Cycle Indicator Fucci expression plasmid (2017/11/15 N.N.)

The 2017 Nobel Prize in Physiology or Medicine (2017.10.03 T.M.)

  • Genetic resources for Circadian clock genes are available.
  • Looking forward to receiving your request.
    Plase visit Circadian clock genes [link].

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