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Clontech Laboratories, Inc. and RIKEN BioResource Center concluded license agreement on preservation and distribution of Fluorescent Proteins, DsRed2 and mCherry for academic use

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  • The 2017 Nobel Prize in Physiology or Medicine (2017.10.03 T.M.)
    • Genetic resources for Circadian clock genes are available.
    • Looking forward to receiving your request.
      Plase visit here for resources [link].

Dnaconda’s Recommendationdnaconda01

Fluorescent Ubiquitination-based Cell Cycle Indicator Fucci expression plasmid (2017/11/15 N.N.)

  • Dr. Atsushi Miyawaki and his colleagues at RIKEN Brain Science Institute (RIKEN BSI) developed Fluorescent Ubiquitination-based Cell Cycle Indicators, Fucci and Fucci2 that allow real-time observation of cell cycle.
  • DNABank provides Fucci2 plasmids for transfection into cells, for targeting into mouse R26 locus, and for establishment of zebrafish strains. Mouse strains and cell lines containing Fucci or Fucci2 are also available from the RIKEN BRC.
  • Reference :
    Fucci – Fluorescent Ubiquitination-based Cell Cycle Indicator [link]
    A window to embryonic development (RIKEN, 2010) [link]
    The color and the shape (RIKEN, 2009) [link]
    Waiting for the light to change (RIKEN, 2008) [link]
    [link]
    Sakaue-Sawano, A. et al. Mol. Cell 68 (3): 626-640.e5, 2017. [PMID: 29107535]
    Mort, R.L. et al. Cell Cycle 13 (17): 2681-2696 ,2014. [PMID: 25486356]
    Sakaue-Sawano, A. et al. BMC. Cell Biol. 12: 2, 2011. [PMID: 21226962]
    Sugiyama, M. et al. Proc. Natl. Acad. Sci. USA. 106 (49): 20812-20817, 2009. [PMID: 19923430]

Distribution of Host E. coli strains for incorporation of synthetic amino acids. (2017/07/25 N.N.)

  • The host E. coli strains, RFzero-iy strain and B-95.deltaA strain that can produce recombinant proteins incorporating synthetic amino acids by reassignment of the UAG codon.[more…]

Evaluation of chaperone mediated autophagy (CMA) activity by the GAPDH-HT indicator. (2017/06/29 N.N.)

  • “GAPDH-HT indicator” is a CMA marker consisting of HaloTag and GAPDH which is a typical substrate of CMA. The CMA activity in the cells can be evaluated by using GAPDH-HT indicator and an appropriate under the fluorescent-labeled HaloTag ligand.[more]

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M-INK, a novel tool for tagging the mature melanosomes.(2017/05/02 N.N.)

  • A novel tool “M-INK” to visualize melanosome in melanocyte and keratinocyte has arrived.[more]

G-CaMP, a fluorescent calcium sensor.(2017/03/16 N.N.)

  • A Fluorescent probes “G-CaMP” for measuring intracellular calcium concentration are available.[more]

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