Clone Search


Resources for

Clontech Laboratories, Inc. and RIKEN BioResource Center concluded license agreement on preservation and distribution of Fluorescent Proteins, DsRed2 and mCherry for academic use

Clones & Vectors for Gene Expression
  Promoter collection
  Epitope tag fusion clones
  SEREX cDNA clone
  HLA antigen cDNA clone
  Nakamura & White RFLP clone

Resources for Gene Analysis
  Cultured microbe
  Cultured animal cell
  Experimental animal
  in vitro experiment
  Plant gene resources

Clone Set & Library
  Genomic clone
  cDNA clone
  Expression clone

Recombinant Virus
  Recombinant Adenovirus
  Shuttle Vector for Recombinant Virus

Genomic DNA
  JCM Microbial Genomic DNA
  BRC Mouse Genomic DNA

Search for resources
  Key word search
  Depositors List
  Gene Set Collection



Dnaconda’s Recommendationdnaconda01

AkaLuc luciferase providing red-shifted luminescence (2018/2/28 T.M.)

Stable ChIP-seq analysis using transcription factors tagged with biotinylated peptide tag. (2018/2/8 K.N.)

  • ChIP-seq is a powerful method of comprehensive analysis of transcription factor (TF) binding sites. However, the bottle neck of ChIP-seq analysis is the quality of antibody, and thus many researchers have been given up. To overcome, authors constructed the vector expressing “biotinylated-tag” transcription factors. With this vector, you can get acceptable level of TF-DNA complex for ChIP-seq analysis by streptavidin-beads for any kinds of TF.[more]

Fluorescent probes for sphingomyelin and cholesterol lipid domains.(2018/02/06 N.N.)

  • Lipid rafts are small lipid domains on the cell membrane and are thought to play an important role in signal transduction, endocytosis and more. Because of the lack of appropriate probes to label sphingomyelin and cholesterol individually, detailed analysis of lipid rafts was difficult.[more]

Hedgehog signal analysis by the Gli recognition element reporter. (2018/2/5 T.M.)

  • To elucidate roles of oxidative stress during the induction of osteoporosis and inhibition of hedgehog signaling, osteoblast differentiation in murine embryonic mesenchymal cell C3H10T1/2 was investigated with a focus on the interaction with mitogen-activated protein kinase (MAPKs) as possible mediator. To monitor Gli-mediated transcription activity in C3H10T1/2 cells under the oxidative stress and application of MAPK inhibitors, luciferase reporter having Gli-transcription factor recognition element was used and the inhibitory effect of JNK1 on Gli transcription was suggested. [more]

Genes promoting hepatocyte differentiation from human iPS cells. (2018/2/5 T.M.)

  • Expression profile of genes was compared between human adult liver and 201B7 cells (iPS cells). Sixteen genes were identified as initial candidates and these expression vectors were constructed. To identify genes promoting hepatocyte differentiation from iPS cells, 210B7 cells ware transfected with these expression vector to screen genes that upregulate alpha-feto protein (AFP) and downregulate Nanog and identified CEBPA, CEBPB, FOXA1 and FOXA3 genes.[more]

Fluorescent Ubiquitination-based Cell Cycle Indicator Fucci expression plasmid (2017/11/15 N.N.)

Previous articles..

Resource Information

Distribution of Host E. coli strains for incorporation of synthetic amino acids. (2017/07/25 N.N.)

  • The host E. coli strains, RFzero-iy strain and B-95.deltaA strain that can produce recombinant proteins incorporating synthetic amino acids by reassignment of the UAG codon.[more…]

Evaluation of chaperone mediated autophagy (CMA) activity by the GAPDH-HT indicator. (2017/06/29 N.N.)

  • “GAPDH-HT indicator” is a CMA marker consisting of HaloTag and GAPDH which is a typical substrate of CMA. The CMA activity in the cells can be evaluated by using GAPDH-HT indicator and an appropriate under the fluorescent-labeled HaloTag ligand.[more]

M-INK, a novel tool for tagging the mature melanosomes.(2017/05/02 N.N.)

  • A novel tool “M-INK” to visualize melanosome in melanocyte and keratinocyte has arrived.[more]

G-CaMP, a fluorescent calcium sensor.(2017/03/16 N.N.)

  • A Fluorescent probes “G-CaMP” for measuring intracellular calcium concentration are available.[more]


Previous articles..