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Genes involved in the sphingolipid signaling pathway


Fluorescent probes for sphingomyelin and cholesterol lipid domains.(2018/02/06 N.N.)

  • Lipid rafts are small lipid domains on the cell membrane and are thought to play an important role in signal transduction, endocytosis and more. Because of the lack of appropriate probes to label sphingomyelin and cholesterol individually, detailed analysis of lipid rafts was difficult.
  • Dr. Toshihide Kobayashi and his colleagues of the former Lipid Biology Laboratory at RIKEN have developed methods to visualize the lipid rafts using probes of lipid binding proteins fused with fluorescent proteins.
  • DNA Bank provides plasmids of the mCherry and GFP fluorescent probes that can label lipid rafts, sphingomyelin and cholesterol. We are looking forward to receiving your request soon.
  • Reference
    Makino, A. et al. FASEB J., 31 (4): 1301-1322, 2017. [PMID: 27492925]
    Makino, A. et al. FASEB J., 29 (2): 477-493, 2015. [PMID: 25389132]
    Shimada, Y. et al. Eur. J. Biochem., 269 (24): 6195-6203, 2002. [PMID: 12473115]
    Mizuno, H. et al. Chem. Sci. 2: 1548-1553, 2011.

 

Catalog no. Name of clone Running title
RDB13496 pET28/EqtII-EGFP-His6 Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of Eqinatoxin II.
RDB13497 pET28/EqtII(8-69)-EGFP-His6 Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Eqinatoxin II.
RDB13498 pET28/His6-EGFP-NT-Lys Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.
RDB13499 pET28/His6-mCherry-NT-Lys Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.
RDB13501 pCAG/tdiRFP-CAAX Marker of plasma membrane. Mammalian expression plasmid of CAAX box protein.
RDB13958 pMAL/MBP-lysenin Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of MBP-tagged lysenin.
RDB13959 pQE30/His6-Venus-NT-Lys Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of His6-Venus-tagged non toxic lysenin.
RDB13960 pQE30/His6-mRFP-NT-Lys Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of His6-mRFP-tagged non toxic lysenin.
RDB13961 pET28/His6-EGFP-D4 Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of His6-EGFP-tagged perfringolysin theta toxin D4 of C. perfringens.
RDB13962 pET28/PlyA2-EGFP-His6 Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged PlyA2.
RDB13963 pET28/Oly-EGFP-His6 Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged Oly.
RDB13964 pET28/EryA-EGFP-His6 Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged EryA.
RDB14300 pET28/His6-mCherry-D4 Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of His6-mCherry-tagged perfringolysin theta toxin D4 of C. perfringens.
RDB14301 pET28/His6-EGFP-Nakanori Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of His6-EGFP-tagged G. frondosa Nakaniori.
RDB15469 pET28/Dronpa-theta-D4 Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of perfringolysin theta toxin D4 of C. perfringens.
RDB15470 pET28/Dronpa-NT-Lys Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.

Link to KEGG Pathway – Sphingolipid signaling pathway

List of gene resources


(GSB0027e, T.M. 2016.10.28)

2018.02.09