Plasmid List

Lentivirus vector top [English]

Lentivirus Vector

Plasmid List

Packaging Plasmids

Cat. no. Plasmid Name Description Data sheet
RDB04394 pCAG-HIVgp CAG promoter, HIV-1 gag and pol [Data sheet]
RDB08256 pCAG-HIVgp IN-D64V CAG promoter, HIV-1 gag and pol, integrase mutant [Data sheet]
RDB08943 pCAG-HIVgp IN-D64E CAG promoter, HIV-1 gag and pol, integrase mutant [Data sheet]
RDB04393 pCMV-VSV-G-RSV-Rev CMV promoter, VSV-G, RSV promoter, Rev [Data sheet]
RDB04392 pCMV-VSV-G CMV promoter, VSV-G [Data sheet]
RDB08121 pRSV-Rev RSV promoter, Rev [Data sheet]

Vector Plasmids

Fluorescent protein gene expression

Cat. no. Plasmid Name Description Data sheet
RDB04379 CS-CDF-CG-PRE CMV promoter, EGFP [Data sheet]
RDB04380 CS-CDF-EG-PRE EF-1α promoter, EGFP [Data sheet]
RDB12968 CS-CDF-PGKG-PRE PGK promoter, EGFP [Data sheet]
RDB08363 CS-CDF-UbCG-PRE UbC promoter, EGFP [Data sheet]
RDB05964 CS-CA-GFP CAG promoter, EGFP [Data sheet]
RDB05553 CSII-CMV-Venus CMV promoter, Venus [Data sheet]
RDB05552 CSII-EF-Venus EF-1α promoter, Venus [Data sheet]
RDB05965 CS-CA-Venus CAG promoter, Venus [Data sheet]
RDB07550 CSII-CMV-Venus-NLS CMV promoter, Venus + nuclear localization signal [Data sheet]
RDB07563 CSII-EF-Venus-NLS EF-1α promoter, Venus + nuclear localization signal [Data sheet]
RDB06386 CSII-CMV-mRFP1 CMV promoter, mRFP1 [Data sheet]
RDB06387 CSII-EF-mRFP1 EF-1α promoter, mRFP1 [Data sheet]
RDB12868 CSII-EF-GFP-IRES-Puro EF-1α promoter, GFP, IRES, Puromycin resistance [Data sheet]
RDB12867 CS-EVBsd EF-1α promoter promoter, Venus, Blasticidin resistance [Data sheet]
RDB08118 CSII-CMV-Venus-Mito-IRES2-Bsd CMV promoter, Venus + mitochondrial targeting sequence, IRES, Blasticidin resistance [Data sheet]

cDNA expression

Cat. no. Plasmid Name Description Data sheet
RDB04377 CSII-CMV-MCS CMV promoter, Multiple cloning site [Data sheet]
RDB04378 CSII-EF-MCS EF-1α promoter, Multiple cloning site [Data sheet]
RDB05963 CS-CA-MCS CAG promoter, Multiple cloning site [Data sheet]
RDB04385 CSII-CMV-MCS-IRES2-Bsd CMV promoter, Multiple cloning site, IRES, Blasticidin resistance [Data sheet]
RDB04383 CSII-CMV-MCS-IRES2-Venus CMV promoter, Multiple cloning site, IRES, Venus [Data sheet]
RDB04384 CSII-EF-MCS-IRES2-Venus EF-1α promoter, Multiple cloning site, IRES, Venus [Data sheet]
RDB07696 CS-UbC-MCS-IRES2-Venus UbC promoter, Multiple cloning site, IRES, Venus [Data sheet]
RDB08257 CSIV-CMV-MCS-IRES2-Venus CMV promoter, Multiple cloning site, IRES, Venus, loxP [Data sheet]
RDB08930 CSIV-UbC-MCS-IRES2-Venus UbC promoter, Multiple cloning site, IRES, Venus, loxP [Data sheet]
RDB07918 CSII-CMV-MCS-IRES2-mRFP1 CMV promoter, Multiple cloning site, IRES, mRFP1 [Data sheet]
RDB07915 CSII-EF-MCS-IRES2-hKO1 EF-1α promoter, Multiple cloning site, IRES, hKO1 [Data sheet]
RDB04386 CSII-CMV-RfA CMV promoter, Gateway [Data sheet]
RDB04387 CSII-EF-RfA EF-1α promoter, Gateway [Data sheet]
RDB04388 CSII-CMV-RfA-IRES2-Venus CMV promoter, Gateway, IRES, Venus [Data sheet]
RDB04389 CSII-EF-RfA-IRES2-Venus EF-1α promoter, Gateway, IRES, Venus [Data sheet]
RDB12870 CSII-EF-RfA-IRES2-hKO1 EF-1α promoter, Gateway, IRES, hKO1 [Data sheet]
RDB12869 CSII-EF-RfA-IRES-Puro EF-1α promoter, Gateway, IRES, Puromycin resistance [Data sheet]
RDB08255 CS-UbC-RfA-IRES2-Venus UbC promoter, Gateway, IRES, Venus [Data sheet]
RDB07894 CS-CA-RfA-IRES2-Venus CAG promoter, Gateway, IRES, Venus [Data sheet]
RDB08070 CSII-CMV-RfA-IRES2-hKO1 CMV promoter, Gateway, IRES, hKO1 [Data sheet]
RDB08350 CS-UbC-RfA-IRES2-hKO1 UbC promoter, Gateway, IRES, hKO1 [Data sheet]

shRNA expression

Cat. no. Plasmid Name Description Data sheet
RDB04395 pENTR4-H1 Gateway entry vector, Human H1 promoter [Data sheet]
RDB04390 CS-RfA-CG Gateway, CMV promoter, EGFP [Data sheet]
RDB12884 CSIV-RfA-CG Gateway, CMV promoter, EGFP, loxP [Data sheet]
RDB04391 CS-RfA-EG Gateway, EF-1α promoter, EGFP [Data sheet]
RDB12885 CS-RfA-UbCG Gateway, UbC promoter, EGFP [Data sheet]
RDB05747 CS-RfA-CMV-mRFP1 Gateway, CMV promoter, mRFP1 [Data sheet]
RDB05736 CS-RfA-EF-mRFP1 Gateway, EF-1α promoter, mRFP1 [Data sheet]
RDB06090 CS-RfA-EVBsd Gateway, EF-1α promoter, Venus-2A-Blasticidin [Data sheet]
RDB08240 CS-CDF-CG-PRE-RfA CMV promoter, EGFP, Gateway in the U3 region [Data sheet]
RDB07722 CS-CDF-EG-PRE-RfA EF-1α promoter, EGFP, Gateway in the U3 region [Data sheet]
RDB08492 CSII-CMV-mRFP1-RfA CMV promoter, mRFP1, Gateway in the U3 region [Data sheet]
RDB12128 CSII-EF-mRFP1-RfA EF-1α promoter, mRFP1, Gateway in the U3 region [Data sheet]
RDB12882 CSIV-RfA Gateway, loxP [Data sheet]

Tet-inducible shRNA expression

Cat. no. Plasmid Name Description Data sheet
RDB07916 pENTR4-H1tetOx1 Gateway entry vector, Human H1 promoter, tet operator [Data sheet]
RDB07917 CS-RfA-ETBsd Gateway, EF-1α promoter, TetR-2A-Blasticidin [Data sheet]
RDB08020 CS-RfA-ETV Gateway, EF-1α promoter, TetR-2A-Venus [Data sheet]
RDB08362 CS-RfA-ETR Gateway, EF-1α promoter, TetR-2A-mRFP1 [Data sheet]

Tet-inducible cDNA expression

Cat. no. Plasmid Name Description Data sheet
RDB12876 CSIV-TRE-RfA-CMV-KT Tet-responsive promoter, Gateway, CMV promoter, hKO1-2A-rtTA, loxP [Data sheet]
RDB12878 CSIV-TRE-RfA-UbC-KT Tet-responsive promoter, Gateway, UbC promoter, hKO1-2A-rtTA, loxP [Data sheet]
RDB12872 CSIV-RfA-TRE-EF-KT Tet-responsive promoter, Gateway, EF-1α promoter, hKO1-2A-rtTA, loxP [Data sheet]

Tet-inducible GFP expression

Cat. no. Plasmid Name Description Data sheet
RDB12875 CSIV-TRE-GFP-CMV-KT Tet-responsive promoter, EGFP, CMV promoter, hKO1-2A-rtTA, loxP [Data sheet]
RDB12877 CSIV-TRE-GFP-UbC-KT Tet-responsive promoter, EGFP, UbC promoter, hKO1-2A-rtTA, loxP [Data sheet]
RDB12871 CSIV-GFP-TRE-EF-KT Tet-responsive promoter, EGFP, EF-1α promoter, hKO1-2A-rtTA, loxP [Data sheet]

For generating iPS cells (Human)

Cat. no. Plasmid Name Description Data sheet
RDB08323 CSIV-CMV-hOct3/4-IRES2-Venus CMV promoter, hOct3/4, IRES, Venus, loxP [Data sheet]
RDB08324 CSIV-CMV-hSox2-IRES2-Venus CMV promoter, hSox2, IRES, Venus, loxP [Data sheet]
RDB08325 CSIV-CMV-hKlf4-IRES2-Venus CMV promoter, hKlf4, IRES, Venus, loxP [Data sheet]
RDB08326 CSIV-CMV-hc-Myc-IRES2-Venus CMV promoter, hc-Myc, IRES, Venus, loxP [Data sheet]
RDB12903 CSIV-CMV-hLin28-IRES2-Venus CMV promoter, hLin28, IRES, Venus, loxP [Data sheet]
RDB08327 CSIV-CMV-SV40LT-IRES2-Venus CMV promoter, SV40 LT, IRES, Venus, loxP [Data sheet]
RDB12908 CSII-EF-hOct3/4-IRES2-Venus EF-1α promoter, hOct3/4, IRES, Venus [Data sheet]
RDB12909 CSII-EF-hSox2-IRES2-Venus EF-1α promoter, hSox2, IRES, Venus [Data sheet]
RDB12910 CSII-EF-hKlf4-IRES2-Venus EF-1α promoter, hKlf4, IRES, Venus [Data sheet]
RDB12911 CSII-EF-hc-Myc-IRES2-Venus EF-1α promoter, hc-Myc, IRES, Venus [Data sheet]
RDB12913 CS-UbC-hOct3/4-IRES2-Venus UbC promoter, hOct3/4, IRES, Venus [Data sheet]
RDB12915 CS-UbC-hSox2-IRES2-Venus UbC promoter, hSox2, IRES, Venus [Data sheet]
RDB12917 CS-UbC-hKlf4-IRES2-Venus UbC promoter, hKlf4, IRES, Venus [Data sheet]
RDB12919 CS-UbC-hc-Myc-IRES2-Venus UbC promoter, hc-Myc, IRES, Venus [Data sheet]
RDB12920 CSIV-TRE-hOct3/4-CMV-KT Tet-responsive promoter, hOct3/4, CMV promoter, hKO1-2A-rtTA, loxP [Data sheet]
RDB12921 CSIV-TRE-hSox2-CMV-KT Tet-responsive promoter, hSox2, CMV promoter, hKO1-2A-rtTA, loxP [Data sheet]
RDB12922 CSIV-TRE-hKlf4-CMV-KT Tet-responsive promoter, hKlf4, CMV promoter, hKO1-2A-rtTA, loxP [Data sheet]
RDB12923 CSIV-TRE-hc-Myc-CMV-KT Tet-responsive promoter, hc-Myc, CMV promoter, hKO1-2A-rtTA, loxP [Data sheet]
RDB12924 CSIV-TRE-hLin28-CMV-KT Tet-responsive promoter, hLin28, CMV promoter, hKO1-2A-rtTA, loxP [Data sheet]
RDB12927 CSIV-TRE-SV40LT-CMV-KT Tet-responsive promoter, SV40 LT, CMV promoter, hKO1-2A-rtTA, loxP [Data sheet]
RDB12946 CS-hp53-shRNA-CG Human H1 promoter, hp53-shRNA, CMV promoter, EGFP [Data sheet]

For generating iPS cells (Mouse)

Cat. no. Plasmid Name Description Data sheet
RDB08513 CSII-CMV-mOct3/4-IRES2-Venus CMV promoter, mOct3/4, IRES, Venus [Data sheet]
RDB08514 CSII-CMV-mSox2-IRES2-Venus CMV promoter, mSox2, IRES, Venus [Data sheet]
RDB08515 CSII-CMV-mKlf4-IRES2-Venus CMV promoter, mKlf4, IRES, Venus [Data sheet]
RDB07992 CSII-CMV-mc-Myc-IRES2-Venus CMV promoter, mc-Myc, IRES, Venus [Data sheet]
RDB12953 CSIV-CMV-mc-Myc-IRES2-Venus CMV promoter, mc-Myc, IRES, Venus, loxP [Data sheet]
RDB12955 CSII-EF-mOct3/4-IRES2-Venus EF-1α promoter, mOct3/4, IRES, Venus [Data sheet]
RDB12960 CS-UbC-mOct3/4-IRES2-Venus UbC promoter, mOct3/4, IRES, Venus [Data sheet]
RDB12954 CSIV-CMV-mOKS-IRES2-Venus CMV promoter, mOct3/4-2A-mKlf4-2A-mSox2, IRES, Venus, loxP [Data sheet]
RDB12961 CSIV-TRE-mOct3/4-CMV-KT Tet-responsive promoter, mOct3/4, CMV promoter, hKO1-2A-rtTA, loxP [Data sheet]
RDB12962 CSIV-TRE-mSox2-CMV-KT Tet-responsive promoter, mSox2, CMV promoter, hKO1-2A-rtTA, loxP [Data sheet]
RDB12963 CSIV-TRE-mKlf4-CMV-KT Tet-responsive promoter, mKlf4, CMV promoter, hKO1-2A-rtTA, loxP [Data sheet]
RDB12964 CSIV-TRE-mc-Myc-CMV-KT Tet-responsive promoter, mc-Myc, CMV promoter, hKO1-2A-rtTA, loxP [Data sheet]
RDB12966 CS-mp53-shRNA-CG Human H1 promoter, mp53-shRNA, CMV promoter, EGFP [Data sheet]

Tet-inducible gene expression

Cat. no. Plasmid Name Description Data sheet
RDB17719 CSIV-TRE-HPV-E6/E7-UbC-KT Tet-responsive promoter, HPV-E6/E7, UbC promoter, hKO1-2A-rtTA, loxP [Data sheet]

Vector plasmids developed from Dr. Miyoshi’s construct

Yoshitomi, H. et al., Nat Commun. 9 (1): 3762 (2018).

Cat. no. Plasmid Name Description Data sheet
RDB17308 CSIIR-EF-MCS-IRES2-Venus EF-1α promoter, Multiple cloning site, IRES, Venus [Data sheet]

Higuchi, M. et al., Stem Cell Reports 7 (6): 1116-1129 (2016).

Cat. no. Plasmid Name Description Data sheet
RDB15324 CS-CMV-Bsd-IRES-GFP CMV promoter, Bsd, IRES, GFP [Data sheet]

Higuchi, M. et al., J. Virol. 81 (21): 11900-11907 (2007).

Cat. no. Plasmid Name Description Data sheet
RDB17866 CSII-EF-IRES-GFP EF-1α promoter, IRES, GFP [Data sheet]
RDB17867 CSII-EF-IRES2-Bsd EF-1α promoter, IRES, Bsd [Data sheet]
RDB17868 CSII-EF-IRES-GFP-RfA Gateway, EF-1α promoter, IRES, GFP [Data sheet]
RDB17869 CSII-EF-IRES2-Bsd-RfA Gateway, EF-1α promoter, IRES, Bsd [Data sheet]

Fucci related lentivirus vector – developed by Dr. Miyawaki’s laboratory

Cell cycle indicator Fucci(CA) and Fucci(SA)

Sakaue-Sawano, A. et al., Mol. Cell 68 (3): 626-640.e5 (2017).

Cat. no. Plasmid Name Description Data sheet
RDB15273 mCherry-hCdt1(30/120) / pCSII-EF EF-1α promoter, hCtd1, mCherry [Data sheet]
RDB15441 mCherry-hCdt1(1/100)Cy(-)/pCSII-EF EF-1α promoter, hCtd1, mCherry [Data sheet]
RDB15442 mCherry-hCdt1(1/100)/pCSII-EF EF-1α promoter, hCtd1, mCherry [Data sheet]
RDB15271 mVenus-hGeminin(1/110) / pCSII-EF EF-1α promoter, hGeminin, mVenus [Data sheet]
RDB15446 tFucci(CA)2/pCSII-EF EF-1α promoter, hCtd1, mCherry, hGeminin, mVenus [Data sheet]
RDB15452 tFucci(CA)2/pCSII-CMV CMV promoter, hCtd1, mCherry, hGeminin, mVenus [Data sheet]
RDB15447 tFucci(CA)2.1/pCSII-EF EF-1α promoter, hCtd1, mCherry, hGeminin, AmCyan [Data sheet]
RDB15453 tFucci(CA)2.1/pCSII-CMV CMV promoter, hCtd1, mCherry, hGeminin, AmCyan [Data sheet]
RDB15448 tFucci(CA)2.2/pCSII-EF EF-1α promoter, hCtd1, mCherry, hGeminin, mTurquoise [Data sheet]
RDB15454 tFucci(CA)2.2/pCSII-CMV CMV promoter, hCtd1, mCherry, hGeminin, mTurquoise [Data sheet]
RDB15443 tFucci(SA)2/pCSII-EF EF-1α promoter, hCtd1, mCherry, hGeminin, mVenus [Data sheet]
RDB15449 tFucci(SA)2/pCSII-CMV CMV promoter, hCtd1, mCherry, hGeminin, mVenus [Data sheet]
RDB15444 tFucci(SA)2.1/pCSII-EF EF-1α promoter, hCtd1, mCherry, hGeminin, AmCyan [Data sheet]
RDB15450 tFucci(SA)2.1/pCSII-CMV CMV promoter, hCtd1, mCherry, hGeminin, AmCyan [Data sheet]
RDB15445 tFucci(SA)2.2/pCSII-EF EF-1α promoter, hCtd1, mCherry, hGeminin, mTurquoise [Data sheet]
RDB15451 tFucci(SA)2.2/pCSII-CMV CMV promoter, hCtd1, mCherry, hGeminin, mTurquoise [Data sheet]

Cell cycle indicator Fucci2

Sakaue-Sawano, A. et al., Chem. Biol. 15 (12): 1243-1248 (2008).
Sakaue-Sawano, A. et al., BMC Cell Biol. 12: 2 (2011).

Cat. no. Plasmid Name Description Data sheet
RDB15273 mCherry-hCdt1(30/120) / pCSII-EF EF-1α promoter, hCtd1, mCherry [Data sheet]
RDB15271 mVenus-hGeminin(1/110) / pCSII-EF EF-1α promoter, hGeminin, mVenus [Data sheet]
RDB15274 AmCyan-hGem(1/110) / pCSII-EF EF-1α promoter, hGeminin, AmCyan [Data sheet]

Cell cycle indicator Fucci

Sakaue-Sawano, A., Cell 132 (3): 487-498 (2008).

Cat. no. Plasmid Name Description Data sheet
RDB15267 mKO2-hCdt1(30/120) / pCSII-EF EF-1α promoter, hCtd1, mKO2 [Data sheet]
RDB15268 mAG-hGeminin(1/110) / pCSII-EF EF-1α promoter, hGeminin, mAG [Data sheet]

Cell cycle indicator

Sakaue-Sawano, A. et al., Chem. Biol. 15 (12): 1243-1248 (2008).

Cat. no. Plasmid Name Description Data sheet
RDB15269 mCherry-hGeminin(1/110) / pCSII-EF EF-1α promoter, hGeminin, mCherry [Data sheet]
RDB15270 mCherry-hGeminin(1/60) / pCSII-EF EF-1α promoter, hGeminin, mCherry [Data sheet]
RDB15272 mVenus-hGeminin(1/60) / pCSII-EF EF-1α promoter, hGeminin, mVenus [Data sheet]

(GRP0068e)

2021.04.26



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