Lentivirus Vector

Plasmid List

Packaging Plasmids

Cat. no.	Plasmid Name	Description	Data sheet
RDB04394	pCAG-HIVgp	CAG promoter, HIV-1 gag and pol	[Data sheet]
RDB08256	pCAG-HIVgp IN-D64V	CAG promoter, HIV-1 gag and pol, integrase mutant	[Data sheet]
RDB08943	pCAG-HIVgp IN-D64E	CAG promoter, HIV-1 gag and pol, integrase mutant	[Data sheet]
RDB04393	pCMV-VSV-G-RSV-Rev	CMV promoter, VSV-G, RSV promoter, Rev	[Data sheet]
RDB04392	pCMV-VSV-G	CMV promoter, VSV-G	[Data sheet]
RDB08121	pRSV-Rev	RSV promoter, Rev	[Data sheet]

Vector Plasmids

Fluorescent protein gene expression

Cat. no.	Plasmid Name	Description	Data sheet
RDB04379	CS-CDF-CG-PRE	CMV promoter, EGFP	[Data sheet]
RDB04380	CS-CDF-EG-PRE	EF-1α promoter, EGFP	[Data sheet]
RDB12968	CS-CDF-PGKG-PRE	PGK promoter, EGFP	[Data sheet]
RDB08363	CS-CDF-UbCG-PRE	UbC promoter, EGFP	[Data sheet]
RDB05964	CS-CA-GFP	CAG promoter, EGFP	[Data sheet]
RDB05553	CSII-CMV-Venus	CMV promoter, Venus	[Data sheet]
RDB05552	CSII-EF-Venus	EF-1α promoter, Venus	[Data sheet]
RDB05965	CS-CA-Venus	CAG promoter, Venus	[Data sheet]
RDB07550	CSII-CMV-Venus-NLS	CMV promoter, Venus + nuclear localization signal	[Data sheet]
RDB07563	CSII-EF-Venus-NLS	EF-1α promoter, Venus + nuclear localization signal	[Data sheet]
RDB06386	CSII-CMV-mRFP1	CMV promoter, mRFP1	[Data sheet]
RDB06387	CSII-EF-mRFP1	EF-1α promoter, mRFP1	[Data sheet]
RDB12868	CSII-EF-GFP-IRES-Puro	EF-1α promoter, GFP, IRES, Puromycin resistance	[Data sheet]
RDB12867	CS-EVBsd	EF-1α promoter promoter, Venus, Blasticidin resistance	[Data sheet]
RDB08118	CSII-CMV-Venus-Mito-IRES2-Bsd	CMV promoter, Venus + mitochondrial targeting sequence, IRES, Blasticidin resistance	[Data sheet]

cDNA expression

Cat. no.	Plasmid Name	Description	Data sheet
RDB04377	CSII-CMV-MCS	CMV promoter, Multiple cloning site	[Data sheet]
RDB04378	CSII-EF-MCS	EF-1α promoter, Multiple cloning site	[Data sheet]
RDB05963	CS-CA-MCS	CAG promoter, Multiple cloning site	[Data sheet]
RDB04385	CSII-CMV-MCS-IRES2-Bsd	CMV promoter, Multiple cloning site, IRES, Blasticidin resistance	[Data sheet]
RDB04383	CSII-CMV-MCS-IRES2-Venus	CMV promoter, Multiple cloning site, IRES, Venus	[Data sheet]
RDB04384	CSII-EF-MCS-IRES2-Venus	EF-1α promoter, Multiple cloning site, IRES, Venus	[Data sheet]
RDB07696	CS-UbC-MCS-IRES2-Venus	UbC promoter, Multiple cloning site, IRES, Venus	[Data sheet]
RDB08257	CSIV-CMV-MCS-IRES2-Venus	CMV promoter, Multiple cloning site, IRES, Venus, loxP	[Data sheet]
RDB08930	CSIV-UbC-MCS-IRES2-Venus	UbC promoter, Multiple cloning site, IRES, Venus, loxP	[Data sheet]
RDB07918	CSII-CMV-MCS-IRES2-mRFP1	CMV promoter, Multiple cloning site, IRES, mRFP1	[Data sheet]
RDB07915	CSII-EF-MCS-IRES2-hKO1	EF-1α promoter, Multiple cloning site, IRES, hKO1	[Data sheet]
RDB04386	CSII-CMV-RfA	CMV promoter, Gateway	[Data sheet]
RDB04387	CSII-EF-RfA	EF-1α promoter, Gateway	[Data sheet]
RDB04388	CSII-CMV-RfA-IRES2-Venus	CMV promoter, Gateway, IRES, Venus	[Data sheet]
RDB04389	CSII-EF-RfA-IRES2-Venus	EF-1α promoter, Gateway, IRES, Venus	[Data sheet]
RDB12870	CSII-EF-RfA-IRES2-hKO1	EF-1α promoter, Gateway, IRES, hKO1	[Data sheet]
RDB12869	CSII-EF-RfA-IRES-Puro	EF-1α promoter, Gateway, IRES, Puromycin resistance	[Data sheet]
RDB08255	CS-UbC-RfA-IRES2-Venus	UbC promoter, Gateway, IRES, Venus	[Data sheet]
RDB07894	CS-CA-RfA-IRES2-Venus	CAG promoter, Gateway, IRES, Venus	[Data sheet]
RDB08070	CSII-CMV-RfA-IRES2-hKO1	CMV promoter, Gateway, IRES, hKO1	[Data sheet]
RDB08350	CS-UbC-RfA-IRES2-hKO1	UbC promoter, Gateway, IRES, hKO1	[Data sheet]

shRNA expression

Cat. no.	Plasmid Name	Description	Data sheet
RDB04395	pENTR4-H1	Gateway entry vector, Human H1 promoter	[Data sheet]
RDB04390	CS-RfA-CG	Gateway, CMV promoter, EGFP	[Data sheet]
RDB12884	CSIV-RfA-CG	Gateway, CMV promoter, EGFP, loxP	[Data sheet]
RDB04391	CS-RfA-EG	Gateway, EF-1α promoter, EGFP	[Data sheet]
RDB12885	CS-RfA-UbCG	Gateway, UbC promoter, EGFP	[Data sheet]
RDB05747	CS-RfA-CMV-mRFP1	Gateway, CMV promoter, mRFP1	[Data sheet]
RDB05736	CS-RfA-EF-mRFP1	Gateway, EF-1α promoter, mRFP1	[Data sheet]
RDB06090	CS-RfA-EVBsd	Gateway, EF-1α promoter, Venus-2A-Blasticidin	[Data sheet]
RDB08240	CS-CDF-CG-PRE-RfA	CMV promoter, EGFP, Gateway in the U3 region	[Data sheet]
RDB07722	CS-CDF-EG-PRE-RfA	EF-1α promoter, EGFP, Gateway in the U3 region	[Data sheet]
RDB08492	CSII-CMV-mRFP1-RfA	CMV promoter, mRFP1, Gateway in the U3 region	[Data sheet]
RDB12128	CSII-EF-mRFP1-RfA	EF-1α promoter, mRFP1, Gateway in the U3 region	[Data sheet]
RDB12882	CSIV-RfA	Gateway, loxP	[Data sheet]

Tet-inducible shRNA expression

Cat. no.	Plasmid Name	Description	Data sheet
RDB07916	pENTR4-H1tetOx1	Gateway entry vector, Human H1 promoter, tet operator	[Data sheet]
RDB07917	CS-RfA-ETBsd	Gateway, EF-1α promoter, TetR-2A-Blasticidin	[Data sheet]
RDB08020	CS-RfA-ETV	Gateway, EF-1α promoter, TetR-2A-Venus	[Data sheet]
RDB08362	CS-RfA-ETR	Gateway, EF-1α promoter, TetR-2A-mRFP1	[Data sheet]

Tet-inducible cDNA expression

Cat. no.	Plasmid Name	Description	Data sheet
RDB12876	CSIV-TRE-RfA-CMV-KT	Tet-responsive promoter, Gateway, CMV promoter, hKO1-2A-rtTA, loxP	[Data sheet]
RDB12878	CSIV-TRE-RfA-UbC-KT	Tet-responsive promoter, Gateway, UbC promoter, hKO1-2A-rtTA, loxP	[Data sheet]
RDB12872	CSIV-RfA-TRE-EF-KT	Tet-responsive promoter, Gateway, EF-1α promoter, hKO1-2A-rtTA, loxP	[Data sheet]

Tet-inducible GFP expression

Cat. no.	Plasmid Name	Description	Data sheet
RDB12875	CSIV-TRE-GFP-CMV-KT	Tet-responsive promoter, EGFP, CMV promoter, hKO1-2A-rtTA, loxP	[Data sheet]
RDB12877	CSIV-TRE-GFP-UbC-KT	Tet-responsive promoter, EGFP, UbC promoter, hKO1-2A-rtTA, loxP	[Data sheet]
RDB12871	CSIV-GFP-TRE-EF-KT	Tet-responsive promoter, EGFP, EF-1α promoter, hKO1-2A-rtTA, loxP	[Data sheet]

For generating iPS cells (Human)

Cat. no.	Plasmid Name	Description	Data sheet
RDB08323	CSIV-CMV-hOct3/4-IRES2-Venus	CMV promoter, hOct3/4, IRES, Venus, loxP	[Data sheet]
RDB08324	CSIV-CMV-hSox2-IRES2-Venus	CMV promoter, hSox2, IRES, Venus, loxP	[Data sheet]
RDB08325	CSIV-CMV-hKlf4-IRES2-Venus	CMV promoter, hKlf4, IRES, Venus, loxP	[Data sheet]
RDB08326	CSIV-CMV-hc-Myc-IRES2-Venus	CMV promoter, hc-Myc, IRES, Venus, loxP	[Data sheet]
RDB12903	CSIV-CMV-hLin28-IRES2-Venus	CMV promoter, hLin28, IRES, Venus, loxP	[Data sheet]
RDB08327	CSIV-CMV-SV40LT-IRES2-Venus	CMV promoter, SV40 LT, IRES, Venus, loxP	[Data sheet]
RDB12908	CSII-EF-hOct3/4-IRES2-Venus	EF-1α promoter, hOct3/4, IRES, Venus	[Data sheet]
RDB12909	CSII-EF-hSox2-IRES2-Venus	EF-1α promoter, hSox2, IRES, Venus	[Data sheet]
RDB12910	CSII-EF-hKlf4-IRES2-Venus	EF-1α promoter, hKlf4, IRES, Venus	[Data sheet]
RDB12911	CSII-EF-hc-Myc-IRES2-Venus	EF-1α promoter, hc-Myc, IRES, Venus	[Data sheet]
RDB12913	CS-UbC-hOct3/4-IRES2-Venus	UbC promoter, hOct3/4, IRES, Venus	[Data sheet]
RDB12915	CS-UbC-hSox2-IRES2-Venus	UbC promoter, hSox2, IRES, Venus	[Data sheet]
RDB12917	CS-UbC-hKlf4-IRES2-Venus	UbC promoter, hKlf4, IRES, Venus	[Data sheet]
RDB12919	CS-UbC-hc-Myc-IRES2-Venus	UbC promoter, hc-Myc, IRES, Venus	[Data sheet]
RDB12920	CSIV-TRE-hOct3/4-CMV-KT	Tet-responsive promoter, hOct3/4, CMV promoter, hKO1-2A-rtTA, loxP	[Data sheet]
RDB12921	CSIV-TRE-hSox2-CMV-KT	Tet-responsive promoter, hSox2, CMV promoter, hKO1-2A-rtTA, loxP	[Data sheet]
RDB12922	CSIV-TRE-hKlf4-CMV-KT	Tet-responsive promoter, hKlf4, CMV promoter, hKO1-2A-rtTA, loxP	[Data sheet]
RDB12923	CSIV-TRE-hc-Myc-CMV-KT	Tet-responsive promoter, hc-Myc, CMV promoter, hKO1-2A-rtTA, loxP	[Data sheet]
RDB12924	CSIV-TRE-hLin28-CMV-KT	Tet-responsive promoter, hLin28, CMV promoter, hKO1-2A-rtTA, loxP	[Data sheet]
RDB12927	CSIV-TRE-SV40LT-CMV-KT	Tet-responsive promoter, SV40 LT, CMV promoter, hKO1-2A-rtTA, loxP	[Data sheet]
RDB12946	CS-hp53-shRNA-CG	Human H1 promoter, hp53-shRNA, CMV promoter, EGFP	[Data sheet]

For generating iPS cells (Mouse)

Cat. no.	Plasmid Name	Description	Data sheet
RDB08513	CSII-CMV-mOct3/4-IRES2-Venus	CMV promoter, mOct3/4, IRES, Venus	[Data sheet]
RDB08514	CSII-CMV-mSox2-IRES2-Venus	CMV promoter, mSox2, IRES, Venus	[Data sheet]
RDB08515	CSII-CMV-mKlf4-IRES2-Venus	CMV promoter, mKlf4, IRES, Venus	[Data sheet]
RDB07992	CSII-CMV-mc-Myc-IRES2-Venus	CMV promoter, mc-Myc, IRES, Venus	[Data sheet]
RDB12953	CSIV-CMV-mc-Myc-IRES2-Venus	CMV promoter, mc-Myc, IRES, Venus, loxP	[Data sheet]
RDB12955	CSII-EF-mOct3/4-IRES2-Venus	EF-1α promoter, mOct3/4, IRES, Venus	[Data sheet]
RDB12960	CS-UbC-mOct3/4-IRES2-Venus	UbC promoter, mOct3/4, IRES, Venus	[Data sheet]
RDB12954	CSIV-CMV-mOKS-IRES2-Venus	CMV promoter, mOct3/4-2A-mKlf4-2A-mSox2, IRES, Venus, loxP	[Data sheet]
RDB12961	CSIV-TRE-mOct3/4-CMV-KT	Tet-responsive promoter, mOct3/4, CMV promoter, hKO1-2A-rtTA, loxP	[Data sheet]
RDB12962	CSIV-TRE-mSox2-CMV-KT	Tet-responsive promoter, mSox2, CMV promoter, hKO1-2A-rtTA, loxP	[Data sheet]
RDB12963	CSIV-TRE-mKlf4-CMV-KT	Tet-responsive promoter, mKlf4, CMV promoter, hKO1-2A-rtTA, loxP	[Data sheet]
RDB12964	CSIV-TRE-mc-Myc-CMV-KT	Tet-responsive promoter, mc-Myc, CMV promoter, hKO1-2A-rtTA, loxP	[Data sheet]
RDB12966	CS-mp53-shRNA-CG	Human H1 promoter, mp53-shRNA, CMV promoter, EGFP	[Data sheet]

Tet-inducible gene expression

Cat. no.	Plasmid Name	Description	Data sheet
RDB17719	CSIV-TRE-HPV-E6/E7-UbC-KT	Tet-responsive promoter, HPV-E6/E7, UbC promoter, hKO1-2A-rtTA, loxP	[Data sheet]

Vector plasmids developed from Dr. Miyoshi’s construct

Ozawa, T. et al., iScience 24 (5): 102488 (2021)

Cat. no.	Plasmid Name	Description	Data sheet
RDB19592	CSII-CA-MCS-IRES-Puro	CAG promoter, Multiple cloning site, IRES, Puro	[Data sheet]
RDB19593	CSII-CA-MCS-IRES-Hyg	CAG promoter, Multiple cloning site, IRES, Hyg	[Data sheet]

Yoshitomi, H. et al., Nat Commun. 9 (1): 3762 (2018).

Cat. no.	Plasmid Name	Description	Data sheet
RDB17308	CSIIR-EF-MCS-IRES2-Venus	EF-1α promoter, Multiple cloning site, IRES, Venus	[Data sheet]

Higuchi, M. et al., Stem Cell Reports 7 (6): 1116-1129 (2016).

Cat. no.	Plasmid Name	Description	Data sheet
RDB15324	CS-CMV-Bsd-IRES-GFP	CMV promoter, Bsd, IRES, GFP	[Data sheet]

Higuchi, M. et al., J. Virol. 81 (21): 11900-11907 (2007).

Cat. no.	Plasmid Name	Description	Data sheet
RDB17866	CSII-EF-IRES-GFP	EF-1α promoter, IRES, GFP	[Data sheet]
RDB17867	CSII-EF-IRES2-Bsd	EF-1α promoter, IRES, Bsd	[Data sheet]
RDB17868	CSII-EF-IRES-GFP-RfA	Gateway, EF-1α promoter, IRES, GFP	[Data sheet]
RDB17869	CSII-EF-IRES2-Bsd-RfA	Gateway, EF-1α promoter, IRES, Bsd	[Data sheet]

Fucci related lentivirus vector – developed by Dr. Miyawaki’s laboratory

Please visit Fucci.

Cell cycle indicator Fucci(CA) and Fucci(SA)

Sakaue-Sawano, A. et al., Mol. Cell 68 (3): 626-640.e5 (2017).

Cat. no.	Plasmid Name	Description	Data sheet
RDB15273	mCherry-hCdt1(30/120) / pCSII-EF	EF-1α promoter, hCtd1, mCherry	[Data sheet]
RDB15441	mCherry-hCdt1(1/100)Cy(-)/pCSII-EF	EF-1α promoter, hCtd1, mCherry	[Data sheet]
RDB15442	mCherry-hCdt1(1/100)/pCSII-EF	EF-1α promoter, hCtd1, mCherry	[Data sheet]
RDB15271	mVenus-hGeminin(1/110) / pCSII-EF	EF-1α promoter, hGeminin, mVenus	[Data sheet]
RDB15446	tFucci(CA)2/pCSII-EF	EF-1α promoter, hCtd1, mCherry, hGeminin, mVenus	[Data sheet]
RDB15452	tFucci(CA)2/pCSII-CMV	CMV promoter, hCtd1, mCherry, hGeminin, mVenus	[Data sheet]
RDB15447	tFucci(CA)2.1/pCSII-EF	EF-1α promoter, hCtd1, mCherry, hGeminin, AmCyan	[Data sheet]
RDB15453	tFucci(CA)2.1/pCSII-CMV	CMV promoter, hCtd1, mCherry, hGeminin, AmCyan	[Data sheet]
RDB15448	tFucci(CA)2.2/pCSII-EF	EF-1α promoter, hCtd1, mCherry, hGeminin, mTurquoise	[Data sheet]
RDB15454	tFucci(CA)2.2/pCSII-CMV	CMV promoter, hCtd1, mCherry, hGeminin, mTurquoise	[Data sheet]
RDB15443	tFucci(SA)2/pCSII-EF	EF-1α promoter, hCtd1, mCherry, hGeminin, mVenus	[Data sheet]
RDB15449	tFucci(SA)2/pCSII-CMV	CMV promoter, hCtd1, mCherry, hGeminin, mVenus	[Data sheet]
RDB15444	tFucci(SA)2.1/pCSII-EF	EF-1α promoter, hCtd1, mCherry, hGeminin, AmCyan	[Data sheet]
RDB15450	tFucci(SA)2.1/pCSII-CMV	CMV promoter, hCtd1, mCherry, hGeminin, AmCyan	[Data sheet]
RDB15445	tFucci(SA)2.2/pCSII-EF	EF-1α promoter, hCtd1, mCherry, hGeminin, mTurquoise	[Data sheet]
RDB15451	tFucci(SA)2.2/pCSII-CMV	CMV promoter, hCtd1, mCherry, hGeminin, mTurquoise	[Data sheet]

Cell cycle indicator Fucci2

Sakaue-Sawano, A. et al., Chem. Biol. 15 (12): 1243-1248 (2008).
Sakaue-Sawano, A. et al., BMC Cell Biol. 12: 2 (2011).

Cat. no.	Plasmid Name	Description	Data sheet
RDB15273	mCherry-hCdt1(30/120) / pCSII-EF	EF-1α promoter, hCtd1, mCherry	[Data sheet]
RDB15271	mVenus-hGeminin(1/110) / pCSII-EF	EF-1α promoter, hGeminin, mVenus	[Data sheet]
RDB15274	AmCyan-hGem(1/110) / pCSII-EF	EF-1α promoter, hGeminin, AmCyan	[Data sheet]

Cell cycle indicator Fucci

Sakaue-Sawano, A., Cell 132 (3): 487-498 (2008).

Cat. no.	Plasmid Name	Description	Data sheet
RDB15267	mKO2-hCdt1(30/120) / pCSII-EF	EF-1α promoter, hCtd1, mKO2	[Data sheet]
RDB15268	mAG-hGeminin(1/110) / pCSII-EF	EF-1α promoter, hGeminin, mAG	[Data sheet]

Cell cycle indicator

Sakaue-Sawano, A. et al., Chem. Biol. 15 (12): 1243-1248 (2008).

Cat. no.	Plasmid Name	Description	Data sheet
RDB15269	mCherry-hGeminin(1/110) / pCSII-EF	EF-1α promoter, hGeminin, mCherry	[Data sheet]
RDB15270	mCherry-hGeminin(1/60) / pCSII-EF	EF-1α promoter, hGeminin, mCherry	[Data sheet]
RDB15272	mVenus-hGeminin(1/60) / pCSII-EF	EF-1α promoter, hGeminin, mVenus	[Data sheet]
RDB15274	AmCyan-hGem(1/110)/pCSII-EF	EF-1α promoter, hGeminin, AmCyan	[Data sheet]

(GRP0068e)

2022.07.22