Clone Search

Resources for

Clones & Vectors for Gene Expression
  in E. coli
  in T. thermophilus HB8
  in S. pombe
  in S. cerevisiae
  in Mammalian Cells

Research Tools
  Genome Editing
  Fluorescent Proteins
  Luminescent Proteins
  Sensor & Visualization
  Plant gene resources

Clone Set, Library & Genomic DNA
  Genomic clone
  cDNA clone
  Expression clone
  Libraries
  Genomic DNA

Recombinant Virus
  Recombinant Adenovirus
  Shuttle Vectors

Gene Set Collection
  Autophagy
  Circadian Clock
  Notch Signaling
  Sphingolipid Signaling

Search and Browse
  Key word search
  Browsing by category
  Browsing by species
  Depositors List

Clontech Laboratories, Inc. and RIKEN BioResource Research Center concluded license agreement on preservation and distribution of Fluorescent Proteins, DsRed2 and mCherry for academic use

BRC News

pCMV_tag and pRSV_tag assay-ready clones


pCMV_tag and pRSV_tag assay-ready clones

catalog no. Name of clone Running title Class Cloning site Seq file
RDB02136 S-HA-pRc/CMV Expressing HA-tagged fusion protein, CMV promoter Vector RDB2136.pdf RDB2136z.seq
RDB02139 D-HA-pRc/CMV Expressing a fusion protein tagged with duplicated HA, CMV promoter Vector RDB2139.pdf RDB2139z.seq
RDB02137 S-T7-pRc/CMV Expressing T7-tagged fusion protein, CMV promoter Vector RDB2137.pdf RDB2137z.seq
RDB02138 D-T7-pRc/CMV Expressing a fusion protein tagged with duplicated T7, CMV promoter Vector RDB2138.pdf RDB2138z.seq
RDB02140 S-Myc-pRc/CMV Expressing Myc-tagged fusion protein, CMV promoter Vector RDB2140.pdf RDB2140z.seq
RDB02141 D-Myc-pRc/CMV Expressing a fusion protein tagged with duplicated Myc epitopes, CMV promoter Vector RDB2141.pdf RDB2141z.seq

Expression vectors listed above are constructed by Dr. Yoshihiro Takemoto of Tokyo Medical and Dental University and published in the DNA Cell Biol. 16 (7): 893-896, 1997. You can select one or two copies of epitope tags among the HA, T7 and Myc. The NotI restriction enzyme site is enable you to create expression vectors tagged your gene of interest with these vectors. After the cloning of your gene, the NotI site also allows inserted gene to be moved to another vectors among these 6 vectors.
Please visit the following site for genetic materials deposited by Dr. Takemoto
go to Head


 

catalog no. Name of clone Running title Class Cloning site Seq file
RDB06083 pRSV_S-FLAG Expressing FLAG-tagged fusion protein, RSV promoter Vector RDB6083.pdf RDB6083z.seq

 

go to Head


 

catalog no. Name of clone Running title Class Cloning site Seq file
RDB05956 pCMV_S-FLAG Expressing FLAG-tagged fusion protein, CMV promoter Vector RDB5956.pdf RDB5956z.seq
RDB06072 pCMFlag_lacZ Expression vector of lacZ, with FLAG-tag, control vector DNA clone RDB6072.pdf
RDB06071 pCMFlag_EGFP Expression vector of GFP, with FLAG-tag, control vector DNA clone RDB6072.pdf

pCMV_S-FLAG vector is a mammalian expression vector designed to express proteins with FLAG epitope tag (Met-DYKDDDDK). The NotI restriction enzyme site is enable you to create expression vectors tagged your gene of interest. The first ATG of the FLAG tag is included in the NcoI restriction enzyme site (CCATGG) and the NcoI site is available for cloning in-frame the FLAG tag and your interested gene together into bacterial expression vectors. The EcoRI site abutting upon the FLAG tag allows to excite your interested gene with the FLAG tag. PCR products starting with ATG codon can be cloned in-flame into the blunted NotI restriction site.

 


 

catalog no. Name of clone Running title Class Cloning site Seq file
RDB07396 pCMV_s-FLAGc Destination vector to generate eukaryotic expression vector, with FLAG-tag Vector RDB7396.pdf RDB7396z.seq

pCMV_s-FLAGc vector is a derivative of the pCMV_S-FLAG vector. GatewayR technology offers one-step cloning of your interested gene in Entry clones into the pCMV_s-FLAGc expression vector. Please refer the Entry clones from the Genome Network Project Human cDNA.
 
go to Head


(GRP0049e, 2007.12.05. T.M.)

2018.12.14