| Mammalian | shRNA expression | in vitro transcrioption | Supernova series | Gene amplification | Insect cell | Plant |
See also “Viral & Transposon Vector Plasmids (backbone)“.
Mammalian expression
| Catalog num. Clone name Description |
Epitope tag | Antibiotic/visual marker | Promoter | Cloning | Antibiotics (bacteria) |
|---|---|---|---|---|---|
| RDB07939 pEF-BOS Mammalian expression vector with human EF-1a. |
n.a. | n.a. | EF-1 alpha | Restriction enzyme | Amp |
| RDB07940 pEF-BOS-EX Mammalian expression vector with human EF-1a. |
n.a. | n.a. | EF-1 alpha | Restriction enzyme | Amp | RDB18970 pNEF-BOS-EX Mammalian expression vector based on human EF-1 alpha promoter with G418-resistant gene. |
n.a. | G418 | EF-1 alpha | Restriction enzyme | Amp |
| RDB18971 pPEF-BOS-EX Mammalian expression vector based on human EF-1 alpha promoter with puromycin-resistant gene. |
n.a. | puromycin | EF-1 alpha | Restriction enzyme | Amp |
| RDB18972 pEF-Flag-EX Mammalian expression vector based on human EF-1 alpha promoter for an N-terminally Flag tagged recombinant protein. |
FLAG | n.a. | EF-1 alpha | Restriction enzyme | Amp |
| RDB18973 pEF321-Flag-Ex Mammalian expression vector based on human EF-1 alpha promoter. |
FLAG | n.a. | EF-1 alpha | Restriction enzyme | Amp |
| RDB18974 pEF-Fc Expression vector of secreted recombinant chimeric protein for the extracellular region of a membrane protein with human Immunoglobulin heavy chain region. |
n.a. | n.a. | EF-1 alpha | Restriction enzyme | Amp |
| Catalog num. Clone name Description |
Epitope tag | Antibiotic/visual marker | Promoter | Cloning | Antibiotics (bacteria) |
|---|---|---|---|---|---|
| RDB14213 pcDNA3.1-myc-GFP Expression vector of N-terminally myc-GFP-tagged proteins in mammalian cells. |
myc-GFP | Neo | CMV | Restriction enzyme | Amp |
| RDB14401 pCIneoEGFPC2 Plasmid vector for constructing GFP-tagged expression vector. |
EGFP | Neo | CMV | Restriction enzyme | Amp |
| RDB05956 pCMV_S-FLAG Expressing FLAG-tagged fusion protein, CMV promoter. |
FLAG | Neo | CMV | Restriction enzyme | Amp |
| RDB06083 pRSV_S-FLAG Expressing FLAG-tagged fusion protein, RSV promoter. |
FLAG | Neo | RSV | Restriction enzyme | Amp |
| RDB07396 pCMV_S-FLAGc Destination vector to generate eukaryotic expression vector, with FLAG-tag. |
FLAG | Neo | CMV | Gateway(R) | Amp |
| RDB07397 pRSV_s-FLAGc Destination vector to generate eukaryotic expression vector, with FLAG-tag. |
FLAG | Neo | RSV | Gateway(R) | Amp | RDB14218 pcDNA5-FLAG/HA/SBP Expression vector of N-terminally FLAG/HA/SBP tagged proteins in mammalian cells. |
FLAG/HA/SBP | Hygro | CMV | Restriction enzyme | Amp |
| RDB10461 pBRHCAG-cHA-pA Expression vector with CAG promoter, HA-tag, hygromycin selection (hph). |
HA | Hygro | CAG | Restriction enzyme | Amp |
| RDB02136 S-HA-pRc/CMV Expressing HA-tagged fusion protein, CMV promoter. |
HA | Neo | CMV | Restriction enzyme | Amp |
| RDB02139 D-HA-pRc/CMV Expressing a fusion protein tagged with duplicated HA, CMV promoter. |
2xHA | Neo | CMV | Restriction enzyme | Amp |
| RDB07105 pBRPyCAG-cHA-IN Expression vector with CAG promoter, HA-tag, Neoycin selection. |
HA | Neo | CAG | Restriction enzyme | Amp |
| RDB11391 pPyCAG-cHA-IN Expression vector with CAG promoter, HA-tag, Neomycin selection. |
HA | Neo | CAG | Restriction enzyme | Amp |
| RDB12114 pPyCAG-cHA-IpacflexEGFP Expression vector with CAG promoter, HA-tag, Puromycin selection. GFP marker. |
HA | Puro & EGFP | CAG | Restriction enzyme | Amp |
| RDB07101 pBRPyCAG-cHA-IP Expression vector with CAG promoter, HA-tag, Puromycin selection. |
HA | Puro | CAG | Restriction enzyme | Amp |
| RDB07100 pPyCAG-cHA-IP Expression vector with CAG promoter, HA-tag, Puromycin selection. |
HA | Puro | CAG | Restriction enzyme | Amp |
| RDB10479 pPyPGK-cHA-IP Expression vector with mouse PGK promoter, HA-tag, IRESpuro. |
HA | Puro | PGK | Restriction enzyme | Amp |
| RDB10460 pBR-PyCAG-cHA-IP SVOri Expression vector with CAG promoter, HA-tag, Puromycin selection. |
HA | Puro | CAG | Restriction enzyme | Amp |
| RDB10435 pBR-CAG-cHA-pA Expression vector with CAG promoter, HA-tag. |
HA | n.a. | CAG | Restriction enzyme | Amp |
| RDB02140 S-Myc-pRc/CMV Expressing Myc-tagged fusion protein, CMV promoter. |
Myc | Neo | CMV | Restriction enzyme | Amp |
| RDB02141 D-Myc-pRc/CMV Expressing a fusion protein tagged with duplicated Myc epitopes, CMV promoter. |
2xMyc | Neo | CMV | Restriction enzyme | Amp |
| RDB02137 S-T7-pRc/CMV Expressing T7-tagged fusion protein, CMV promoter. |
T7 | Neo | CMV | Restriction enzyme | Amp |
| RDB02138 D-T7-pRc/CMV Expressing a fusion protein tagged with duplicated T7, CMV promoter. |
2xT7 | Neo | CMV | Restriction enzyme | Amp |
| RDB12113 pPyCAG-BstXI-ZeoP2AEGFP Expression vector with CAG promoter, HA-tag, Bleomycin (Zeo) selection. GFP marker. |
n.a. | Bleo(Zeo) & EGFP | CAG | Restriction enzyme(BstXI) | Amp |
| RDB09315 pPyCAG-IRESzeocinpA (+SVori) Expression vector with CAG promoter, IRES-zeo. |
n.a. | Bleo(Zeo) | CAG | Restriction enzyme (BstXI) | Amp |
| RDB09297 pZPCAGGS Expression vector with CAG promoter, Zeo marker. |
n.a. | Bleo(Zeo) | CAG | Restriction enzyme (BstXI) | Amp |
| RDB12107 pPyCAG-BstXI-IB Mammalian expression vector. Blasticidin resistance. |
n.a. | BSD | CAG | Restriction enzyme(BstXI) | Amp |
| RDB12112 pPyCAG-BstXI-IHisDflexEGFP Expression vector with CAG promoter, HA-tag, HisD selection. GFP marker. |
n.a. | HisD & EGFP | CAG | Restriction enzyme(BstXI) | Amp |
| RDB10322 pPyCAG-BstXI-IRSHisDpA Expression vector of IRES-HisD with CAG promoter. |
n.a. | HisD | CAG | Restriction enzyme (BstXI) | Amp |
| RDB12108 pPyCAG-BstXI-HygP2AEGFP Expression vector with CAG promoter, HA-tag, Hygromycin selection. GFP marker. |
n.a. | Hygro & EGFP | CAG | Restriction enzyme(BstXI) | Amp |
| RDB09233 pPyCAG-BstXI-IREShygropA Expression vector of CAG promoter, IRES-hygromycin resistance. |
n.a. | Hygro | CAG | Restriction enzyme(BstXI) | Amp |
| RDB09694 pCAGGSneo-delta-EcoRI Expression vector with CAG promoter and b-globin poly(A) (with SV40 ori), neo marker. |
n.a. | Neo | CAG | Restriction enzyme | Amp |
| RDB07099 pPyCAG-BstXI-IP Mammalian expression vector. Blasticidin resistance. |
n.a. | Puro | CAG | Restriction enzyme(BstXI) | Amp |
| RDB01968 pBMSA Expression vector, inducible MMTV-LTR promoter. |
n.a. | n.a. | MMTV-LTR | Restriction enzyme (XbaI) | Amp |
| RDB08938 pCAGGS Mammalian expression vector with CAG promoter. |
n.a. | n.a. | CAG | Restriction enzyme | Amp |
| RDB14136 pIDT-SMART(C-TSC) Mammalian xpression vector with the improved high expression cassette C-TSC. |
n.a. | n.a. | CMV-TSC | Restriction enzyme | Kan |
| RDB19684 pMWcmvT7-KpnI-pA Infusion/Gibson assembly platform vector for dual ussage mammalian expression and T7 In Vitro Transcription. Bacterial strictly low copy number plasmid (pMW219 backbone). |
n.a. | n.a. | CMV | Infusion/Gibson assembly | Kan |
| RDB19683 pBRcmvT7-KpnI-pA Infusion/Gibson assembly platform vectorfor dual ussage mammalian expression and T7 In Vitro Transcription. Bacterial relatively low copy number plasmid (pBR322 backbone). Tetracyclin resistance gene has been truncated. |
n.a. | n.a. | CMV | Infusion/Gibson assembly | Amp |
| RDB19682 pcmvT7-KpnI-polyA Infusion/Gibson assembly platform vector for dual ussage mammalian expression and T7 In Vitro Transcription. Bacterial high copy number plasmid (pCDNA3 backbone). |
n.a. | Neo | CMV | Infusion/Gibson assembly | Amp |
| Catalog num. | Clone name | Description |
|---|---|---|
| RDB01379 | pH2Rneo | Expression vector with simian virus 40 (SV40) early promoter |
| RDB01679 | pCALwL | A cassette plasmid to insert cDNA into downstream CAG promoter |
| RDB01862 | pCALNL5 | A cassette to introduce cDNA into mammalian chromosome |
Vector for marker knock-in (genome editing)
- Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors.
Natsume, T., Kiyomitsu, T., Saga, Y., Kanemaki, M.T.
Cell Rep. 15 (1): 210-218 (2016). PubMed PMID 27052166.Selection marker Attached tag at the N-terminus mClover mCherry2 S tag-3FLAG Hygro pMK376 pMK377 pMK375 Selection marker Attached tag at the C-terminus mClover mCherry2 S tag-3FLAG Neo pMK277 pMK280 pMK283 Hygro pMK278 pMK281 pMK284 BSD pMK388 pMK389 pMK390 Bsr pMK279 pMK282 pMK285 Please refer Fig. 4 of Natsume, T., Cell Reports 15, 210-218 (2016) for an instruction about how to use these clones.
shRNA expression
| Catalog num. | Clone name | Description |
|---|---|---|
| RDB04395 | pENTR4-H1 | Expression vector of RNAi driven by human H1 promoter. |
| RDB07916 | pENTR4-H1tetOx1 | Expression vector of RNAi driven by human H1 promoter. |
See also Gateway® destination vector in “Lentivirus Vector Plasmid“.
in vitro transcrioption
- Highly efficient induction of primate iPS cells by combining RNA transfection and chemical compounds.
Watanabe, T., Yamazaki, S., Yoneda, N., Shinohara, H., Tomioka, I., Higuchi, Y., Yagoto, M., Ema, M., Suemizu, H., Kawai, K., Sasaki, E.
Genes Cells 24 (7): 473-484 (2019). PubMed PMID 31099158.Catalog num. Clone name Description RDB18330 phBG Plasmid vector for in-vitro transcrioption, having two copies of 3′ UTR DNA fragment of human hemoglobin subunit beta (HBB)
Supernova series
Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo.
Luo, W., Mizuno, H., Iwata, R., Nakazawa, S., Yasuda, K., Itohara, S,, Iwasato, T.
Sci. Rep. 6: 35747 (2016). PubMed PMID 27775045.
NMDAR-regulated dynamics of layer 4 neuronal dendrites during thalamocortical reorganization in neonates.
Mizuno, H., Luo, W., Tarusawa, E., Saito, Y.M., Sato, T., Yoshimura, Y., Itohara, S., Iwasato, T.
Neuron 82 (2): 365-379 (2014). PubMed PMID 24685175.
| Catalog # | Name of clone | Running title |
| RDB15043 | pK031.TRE-Cre (Supernova) | Cre-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Cre recombinase. |
| RDB15045 | pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova) | Cre-based Supernova series. Expression vector of loxP-stop-loxP-EGFP under the CAG promoter. |
| RDB15046 | pK036.TRE-Flpe-WPRE (Supernova) | Flippase (Flpe)-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Flpe recombinase. |
| RDB15047 | pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova) | Flippase (Flpe)-based Supernova series. Expression vector of FRT-stop-FRT-EGFP under the CAG promoter. |
| RDB15050 | pK170.AAV-TRE-Cre-WPRE (Supernova) | Adeno-associated virus (AAV)-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Cre recombinase. |
| RDB15051 | pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova) | Supernova series, a versatile vector system for single-cell labeling in vivo. Expression vector of loxP-stop-loxP-Cas9 under the CAG promoter. |
| RDB15053 | pK073.TRE-Dre-WPRE (Supernova) | Dre-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Dre recombinase. |
| RDB15055 | pK224.CAG-Rox-STOP-Rox-GFP-ires-tTA-WPRE (Supernova) | Dre-based Supernova series. Expression vector of Rox-stop-Rox-GFP under the CAG promoter. |
| RDB15056 | pK039.CAG-loxP-stop-loxP-AmCyan-ires-tTA-WPRE (Supernova) | Cre-based Supernova series. Cre-mediated recombination, a loxP-stop-loxP is excised and the downstream AmCyan is expressed. |
| RDB15057 | pK238.U6-gRNACreb1-CAG-loxP-stop-loxP-hspCas9 (Supernova) | Supernova series, a versatile vector system for single-cell labeling in vivo. Expression vector of both loxP-stop-loxP-Cas9 and gRNA (Creb1 as the target gene) under the CAG promoter. |
| RDB15058 | pK177.CAG-loxP-stop-loxP-mir30 (GFP RNAi) (Supernova) | Cre-based Supernova series, a supernova-mediated RNAi enables sparsely labeled cell-specific gene knockdown without using genetically altered mice. |
| RDB15059 | pK178.CAG-loxP-stop-loxP-mir30 (GFP RNAi scramble control) (Supernova) | Cre-based Supernova series, a supernova-mediated RNAi enables sparsely labeled cell-specific gene knockdown without using genetically altered mice. |
| RDB15061 | pK229.AAV-EF1alpha-EGFP-WPRE (Supernova) | Adeno-associated virus (AAV)-based Supernova series. This clone will be used as a control in AAV-based Supernova experiments. |
Plasmid for IR/MAR gene amplification method and related plasmid.
| Catalog # | Name of resource | Description |
|---|---|---|
| RDB18704 | pSFVdhfr | A clone for the IR/MAR gene amplification method. Co-transfected expression vectors with this clone can be amplified in the transfected cells. |
| RDB18705 | pdeltaBN.AR1 | A clone for the IR/MAR gene amplification method. Co-transfected expression vectors with this clone can be amplified in the transfected cells. |
| RDB18706 | pdeltaBM.AR1.MCS | A clone for the IR/MAR gene amplification method. By cloning an expression unit of interested gene into the MCS, amplified gene expression can be obtained. |
| RDB18707 | pdeltaBM.AR1.d2EGFP | A clone for the IR/MAR gene amplification method. This clone is used for monitoring the gene amplification by observation of GFP fluorescence. |
| RDB18708 | pB-3-31 | Plasmid clone of 3,271 bp human genomic sequence, B-3-31, which is known to enhance the expression of co-transfected expression vectors. |
| RDB18709 | pG5 | Plasmid clone of 972 bp human G5 sequence, which was isolated from beta-globin replication origin as a minimum sequence required for gene amplification. |
| RDB18710 | pC12 | Plasmid clone of 798 bp human C12 sequence, which was isolated from MYC replication origin as a minimum sequence required for gene amplification. |
| RDB18711 | pD8 | Plasmid clone of 2,347 bp Chinese hamster D8 sequence, which was isolated from DHFR replication origin as a minimum sequence required for gene amplification. |
- Araki, Y., Efficient recombinant production in mammalian cells using a novel IR/MAR gene amplification method. PLoS One, 7 (7): e41787 (2012). PMID 22844523. (original). [link to RRC of NBRP]
- Fukuma, M., Cloning and Characterization of a Human Genomic Sequence that Alleviates Repeat-Induced Gene Silencing. PLoS One 11 (4): e0153338 (2016). PMID 27078685. (original). [link to RRC of NBRP]
- Harada, S., Episomal high copy number maintenance of hairpin-capped DNA bearing a replication initiation region in human cells. J. Biol. Chem. 284 (36): 24320-24327 (2009). PMID 19617622. (original). [link to RRC of NBRP]
- Hashizume, T., Dissection of mammalian replicators by a novel plasmid stability assay. J. Cell Biochem. 101 (3): 552-565 (2007). PMID 17226771. (original). [link to RRC of NBRP]
- Okada, N., Dissection of the beta-globin replication-initiation region reveals specific requirements for replicator elements during gene amplification. PLoS One 8 (10): e77350 (2013). PMID 24124615. (original). [link to RRC of NBRP]
- Shimizu, N., Plasmids with a mammalian replication origin and a matrix attachment region initiate the event similar to gene amplification. Cancer Res. 61 (19): 6987-6990 (2001). PMID 11585721. (original). [link to RRC of NBRP]
- Shimizu, N., Amplification of plasmids containing a mammalian replication initiation region is mediated by controllable conflict between replication and transcription. Cancer Res. 63 (17): 5281-5290 (2003). PMID 14500359. (original). [link to RRC of NBRP]
Insect Cell Experiment
| Catalog num. Clone name Description |
Epitope tag | Antibiotic/visual marker | Cloning | Promoter | Bacterial resistance |
|---|---|---|---|---|---|
| RDB08532 pMT-PURO Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells. |
His-V5 | Puro | Restriction enzyme | MT | Amp |
| RDB12152 pMT-PURO2 Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells |
His-V5 | Puro | Restriction enzyme | MT | Amp |
| RDB09002 pMAK17 Expression clone of EGFP under the control of tet inducible promoter. Puromycin serectable. |
– | Puro & DsRed2 | – | tight TRE | Amp |
| RDB09003 pMAK42 Expression vector under the control of tet inducible promoter. Puromycin serectable. |
– | Puro & DsRed2 | Restriction enzyme | tight TRE | Amp |
| RDB09004 pMT-PURO2G Metametallothionein promoter expression vector with EGFP marker for Schneider 2 (S2) Drosophila cells |
His-V5 | Puro & EGFP | Restriction enzyme | MT | Amp |
| RDB09005 pMT-PURO2R Metametallothionein promoter expression vector with DsRED2 marker for Schneider 2 (S2) Drosophila cells |
His-V5 | Puro& DsRed | Restriction enzyme | MT | Amp |
| RDB08531 pCoPURO Copia promoter expression vector of puromycin resistant gene for Schneider 2 (S2) Drosophila cells. |
n.a. | Puro | n.a. | copia | Amp |
| RDB08035 pUAST-GFPN-lacZ P-element mediated gene transfer vector of GFP-N and lacZ. |
n.a. | GFP-lacZ | n.a. | Gal4-UAS | Amp |
Plant Experiment
| Catalog num. | Clone name | Description |
|---|---|---|
| RDB02933 | pBAc3 | pBAc3 enhancer trap vector in rice. |
| RDB08246 | pCIYVV-Pst/CP | Plant expression vector constructed from clover yellow vein virus. |
Please visit the Experimental Plant Division for more plant DNA clones such as pGWB vectors developed by Prof. Nakagawa Lab.
2025.05.03 (T.M.)
