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Takara Bio USA, Inc. and RIKEN BioResource Research Center concluded license agreement on preservation and distribution of Fluorescent Proteins, such as DsRed2 and mCherry for academic use

BRC News

Organelle marker

このエントリーをはてなブックマークに追加

Organelle marker

We are providing genetic resources for visualization of organelles such as mitochondria and nucleus. Each clone contains an organelle localization signal sequence fused with fluorescent proteins or epitope tags. Organelles can be detected by fluorescence or by detecting epitope tags with antibodies.
Of those, 12 species of organelles can be observed by Nano-lantern luminescent and fluorescent proteins developed by Drs. Okada and Takai of the RIKEN Center for Biosystems Dynamics Research (BDR) and Nagai and their colleague of the Osaka University [Takai, A. et al., Proc Natl Acad Sci U S A. 112 (14): 4352-4356, 2015]. Nano-lantern is a brighter luminescent protein, consisting of luciferase and fluorescent protein. Three colors (yellow, cyan and orange) of Nano-lanterns are available for each localization signal.

  • Beta-tubulin
    • Nano-lantern clones
      Catalog no. Name of clone Running title
      RDB13338 pYNL-N1_TUBB5 Yellow Nano-lantern for labeling of beta-tubulin by the mouse beta-tubulin class V.
      RDB13339 pCNL-N1_TUBB5 Cyan Nano-lantern for labeling of beta-tubulin by the mouse beta-tubulin class V.
      RDB13340 pONL-N1_TUBB5 Orange Nano-lantern for labeling of beta-tubulin by the mouse beta-tubulin class V.
  • Cytoskeleton and focal adhesion
    • Nano-lantern clones
      Catalog no. Name of clone Running title
      RDB13350 pYNL-N1_vinculin Yellow Nano-lantern with the chicken vinculin.
      RDB13351 pCNL-N1_vinculin Cyan Nano-lantern with the chicken vinculin.
      RDB13352 pONL-N1_vinculin Orange Nano-lantern with the chicken vinculin.
  • Dense-core vesicles
    • Fluorescent protein porbe
      Catalog no. Name of clone Running title
      RDB15118 pNPY-Venus-N1 Venus fluorescent protein targeting localization in neurites. Mammalian expression.
      RDB15119 NPY-Venus/pEF-BOS Venus fluorescent protein targeting localization in neurites. Mammalian expression.
  • F-actin
    • Nano-lantern clones
      Catalog no. Name of clone Running title
      RDB13347 pYNL-N1_ITPKA Yellow Nano-lantern for labeling of F-actin by the rat inositol triphosphate 3-kinase A.
      RDB13348 pCNL-N1_ITPKA Cyan Nano-lantern for labeling of F-actin by the rat inositol triphosphate 3-kinase A.
      RDB13349 pONL-N1_ITPKA Orange Nano-lantern for labeling of F-actin by the rat inositol triphosphate 3-kinase A.
  • Focal adhesions
    • Nano-lantern clones
      Catalog no. Name of clone Running title
      RDB13341 pYNL-N1_zyxin Yellow Nano-lantern for labeling of focal adhesions by the mouse zyxin.
      RDB13342 pCNL-N1_zyxin Cyan Nano-lantern for labeling of focal adhesions by the mouse zyxin.
      RDB13343 pONL-N1_zyxin Orange Nano-lantern for labeling of focal adhesions by the mouse zyxin.
  • Golgi apparatus
    • Epitope-tagged protein probe
      Catalog no. Name of clone Running title
      RDB03423 FLAG-Rab27A Expression vector of mouse Rab27A cDNA which can be used as secretory granule and melanosomme markers.
    • Fluorescent protein porbe
      Catalog no. Name of clone Running title
      RDB13353 pEGFP-C1-mouse Rab33B Expression vector of mouse RAB33B, N-terminal EGFP-tag.
    • Nano-lantern clones
      Catalog no. Name of clone Running title
      RDB13353 pYNL-N1_Golgi Yellow Nano-lantern for labeling of Golgi apparatus by the N-terminal 81 amino acids of human beta 1,4-galactosyltransferase.
      RDB13354 pCNL-N1_Golgi Cyan Nano-lantern for labeling of Golgi apparatus by the N-terminal 81 amino acids of human beta 1,4-galactosyltransferase.
      RDB13355 pONL-N1_Golgi Orange Nano-lantern for labeling of Golgi apparatus by the N-terminal 81 amino acids of human beta 1,4-galactosyltransferase.
  • Kinetochore
    • Fluorescent protein porbe
      Catalog no. Name of clone Running title
      RDB13516 pGEMHE-mHec1-sfGFP Labeling outer kinetochore. Plasmid for in vitro transcription of mouse Hec1 (Ndc80) in mouse oocyte.
      RDB13518 pGEMHE-tdTomato-mCENP-C Labeling inner kinetochore. Plasmid for in vitro transcription of mouse CENP-C in mouse oocyte.
  • Lysosome
    • Nano-lantern clones
      Catalog no. Name of clone Running title
      RDB13344 pYNL-N1_LAMP1 Yellow Nano-lantern for labeling of lysosome by the rat LAMP1.
      RDB13345 pCNL-N1_LAMP1 Cyan Nano-lantern for labeling of lysosome by the rat LAMP1.
      RDB13346 pONL-N1_LAMP1 Orange Nano-lantern for labeling of lysosome by the rat LAMP1.
    • Related article
  • Melanosome
  • Mitochondria
    • Epitope-tagged protein probe
      Catalog no. Name of clone Running title
      RDB17747 pN1-OPA1(123Nt)-Halo Halo Tag, Mitochondrial intermembrane space localization
      RDB17750 pN1-TOMM20-Halo Halo Tag, Mitochondrial outer membrane localization
    • Fluorescent protein probe
      Catalog no. Name of clone Running title
      RDB15238 mt-mKeima (h)/pIND(SP1) Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal.
      RDB15456 mt-mKeima/pcDNA3 Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal.
    • Nano-lantern clones
      Catalog no. Name of clone Running title
      RDB13329 pYNL-N1_Mito Yellow Nano-lantern for labeling of mitochondria by the mitochondrial targeting sequence from subunit VIII of human cytochrome c oxdase.
      RDB13330 pCNL-N1_Mito Cyan Nano-lantern for labeling of mitochondria by the mitochondrial targeting sequence from subunit VIII of human cytochrome c oxdase.
      RDB13331 pONL-N1_Mito Orange Nano-lantern for labeling of mitochondria by the mitochondrial targeting sequence from subunit VIII of human cytochrome c oxdase.
  • Nuclei
    • Nano-lantern clones
      Catalog no. Name of clone Running title
      RDB13332 pYNL-N1_3xNLS Yellow Nano-lantern for labeling of nuclei by the nuclear localization signal of SV40.
      RDB13333 pCNL-N1_3xNLS Cyan Nano-lantern for labeling of nuclei by the nuclear localization signal of SV40.
      RDB13334 pONL-N1_3xNLS Orange Nano-lantern for labeling of nuclei by the nuclear localization signal of SV40.
  • Nuclei in interphase
    • Nano-lantern clones
      Catalog no. Name of clone Running title
      RDB13362 pYNL-C1_H2B Yellow Nano-lantern for labeling of nuclei in interphase by the human histone H2B.
      RDB13363 pCNL-C1_H2B Cyan Nano-lantern for labeling of nuclei in interphase by the human histone H2B.
      RDB13364 pONL-C1_H2B Orange Nano-lantern for labeling of nuclei in interphase by the human histone H2B.
  • Nucleoli
    • Nano-lantern clones
      Catalog no. Name of clone Running title
      RDB13368 pYNL-C1_fibrillarin Yellow Nano-lantern for labeling of nucleoli by the human fibrillarin.
      RDB13369 pCNL-C1_fibrillarin Cyan Nano-lantern for labeling of nucleoli by the human fibrillarin.
      RDB13370 pONL-C1_fibrillarin Orange Nano-lantern for labeling of nucleoli by the human fibrillarin.
  • Peroxisome
    • Nano-lantern clones
      Catalog no. Name of clone Running title
      RDB13359 pYNL-C1_PTS1 Yellow Nano-lantern for labeling of peroxisome by the peroxisomal targeting signal 1 (tripeptide SKL).
      RDB13335 pYNL-N1_PTS2 Yellow Nano-lantern for labeling of peroxisome by the peroxisomal targeting signal 2.
      RDB13360 pCNL-C1_PTS1 Cyan Nano-lantern for labeling of peroxisome by the peroxisomal targeting signal 1 (tripeptide SKL).
      RDB13336 pCNL-N1_PTS2 Cyan Nano-lantern for labeling of peroxisome by the peroxisomal targeting signal 2.
      RDB13361 pONL-C1_PTS1 Orange Nano-lantern for labeling of peroxisome by the peroxisomal targeting signal 1 (tripeptide SKL).
      RDB13337 pONL-N1_PTS2 Orange Nano-lantern for labeling of peroxisome by the peroxisomal targeting signal 2.
  • Plasma membrane and endosome
    • Epitope-tagged protein probe
      Catalog no. Name of clone Running title
      RDB14677 pEF-BOS FLAG-mouse Rab5A Expression vector of mouse Rab5A, which is localized in early endosome.
      RDB14678 pEF-BOS FLAG-mouse Rab7 Expression vector of mouse Rab7, which is localized in late endosome.
    • Fluorescent protein probe
      Catalog no. Name of clone Running title
      RDB13501 pCAG/tdiRFP-CAAX Marker of plasma membrane. Mammalian expression plasmid of CAAX box protein.
      RDB15030 pmStr-C1-mouse Rab11A Expression vector of mouse Rab11A, Recycling endosome marker.
    • Nano-lantern clones
      Catalog no. Name of clone Running title
      RDB13356 pYNL-C1_RhoB Yellow Nano-lantern for labeling of plasma membrane and endosomes by the human RhoB.
      RDB13357 pCNL-C1_RhoB Cyan Nano-lantern for labeling of plasma membrane and endosomes by the human RhoB.
      RDB13358 pONL-C1_RhoB Orange Nano-lantern for labeling of plasma membrane and endosomes by the human RhoB.
      RDB13365 pYNL-C1_Rab11a Yellow Nano-lantern for labeling of recycling endosomes by the mouse Rab11a.
      RDB13366 pCNL-C1_Rab11a Cyan Nano-lantern for labeling of recycling endosomes by the mouse Rab11a.
      RDB13367 pONL-C1_Rab11a Orange Nano-lantern for labeling of recycling endosomes by the mouse Rab11a.
    • Related article
  • Sphingolipid marker
    • Lipid rafts are small lipid domains on the cell membrane and are thought to play an important role in signal transduction, endocytosis and more. We provide fluorescent probes for sphingomyelin and cholesterol lipid domains.
    • Nakanori: sphingomyelin and cholesterol lipid domain (lipid raft)
      Catalog no. Name of clone Running title
      RDB14301 pET28/His6-EGFP-Nakanori Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of His6-EGFP-tagged G. frondosa Nakaniori.
    • D4 toxin: cholesterol rich domain
      Catalog no. Name of clone Running title
      RDB13961 pET28/His6-EGFP-D4 Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of His6-EGFP-tagged perfringolysin theta toxin D4 of C. perfringens.
      RDB14300 pET28/His6-mCherry-D4 Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of His6-mCherry-tagged perfringolysin theta toxin D4 of C. perfringens.
      RDB15469 pET28/Dronpa-theta-D4 Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of perfringolysin theta toxin D4 of C. perfringens.
    • lysenin: sphingomyelin
      Catalog no. Name of clone Running title
      RDB13498 pET28/His6-EGFP-NT-Lys Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.
      RDB13499 pET28/His6-mCherry-NT-Lys Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.
      RDB13958 pMAL/MBP-lysenin Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of MBP-tagged lysenin.
      RDB13959 pQE30/His6-Venus-NT-Lys Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of His6-Venus-tagged non toxic lysenin.
      RDB13960 pQE30/His6-mRFP-NT-Lys Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of His6-mRFP-tagged non toxic lysenin.
      RDB15470 pET28/Dronpa-NT-Lys Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin.
    • Other markers
      Catalog no. Name of clone Running title
      RDB13496 pET28/EqtII-EGFP-His6 Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of Eqinatoxin II.
      RDB13497 pET28/EqtII(8-69)-EGFP-His6 Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Eqinatoxin II.
      RDB13962 pET28/PlyA2-EGFP-His6 Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged PlyA2.
      RDB13963 pET28/Oly-EGFP-His6 Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged Oly.
      RDB13964 pET28/EryA-EGFP-His6 Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged EryA.
    • Related article

(GRP0053e 2018.04.24 T.M.)

2020.02.05