Visualization of Organelles
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Organelle marker
Visualizing multiple inter-organelle contact sites
FPs with tissue specific promoter
Organelle marker
We are providing genetic resources for visualization of organelles such as mitochondria and nucleus. Each clone contains an organelle localization signal sequence fused with fluorescent proteins or epitope tags. Organelles can be detected by fluorescence or by detecting epitope tags with antibodies.
Of those, 12 species of organelles can be observed by Nano-lantern luminescent and fluorescent proteins developed by Drs. Okada and Takai of the RIKEN Center for Biosystems Dynamics Research (BDR) and Nagai and their colleague of the Osaka University [Takai, A. et al., Proc Natl Acad Sci U S A. 112 (14): 4352-4356, 2015]. Nano-lantern is a brighter luminescent protein, consisting of luciferase and fluorescent protein. Three colors (yellow, cyan and orange) of Nano-lanterns are available for each localization signal.
Actin (β-actin)
- Fluorescent protein porbe
Catalog no. |
Name of clone |
Descriptiion |
RDB18314 |
pActin-mAG1 |
CoralHue™ β-Actin-targeted monomeric Azami-Green 1 |
RDB18319 |
pActin-hmKO1 |
CoralHue™ β-Actin-targeted monomeric Kusabira-Orange 1 |
Actin (F-actin)
- Fluorescent protein probe
- Nano-lantern clones
Catalog no. |
Name of clone |
Descriptiion |
RDB13348 |
pCNL-N1_ITPKA |
Cyan Nano-lantern for labeling of F-actin by the rat inositol triphosphate 3-kinase A. |
RDB13347 |
pYNL-N1_ITPKA |
Yellow Nano-lantern for labeling of F-actin by the rat inositol triphosphate 3-kinase A. |
RDB13349 |
pONL-N1_ITPKA |
Orange Nano-lantern for labeling of F-actin by the rat inositol triphosphate 3-kinase A. |
Cytoskeleton and focal adhesion
- Nano-lantern clones
Catalog no. |
Name of clone |
Descriptiion |
RDB13351 |
pCNL-N1_vinculin |
Cyan Nano-lantern with the chicken vinculin. |
RDB13350 |
pYNL-N1_vinculin |
Yellow Nano-lantern with the chicken vinculin. |
RDB13352 |
pONL-N1_vinculin |
Orange Nano-lantern with the chicken vinculin. |
Dense-core vesicles
- Fluorescent protein porbe
Catalog no. |
Name of clone |
Descriptiion |
RDB15118 |
pNPY-Venus-N1 |
Venus fluorescent protein targeting localization in neurites. Mammalian expression. |
RDB15119 |
NPY-Venus/pEF-BOS |
Venus fluorescent protein targeting localization in neurites. Mammalian expression. |
Endoplasmic reticulum
- Fluorescent protein porbe
Catalog no. |
Name of clone |
Descriptiion |
RDB19411 |
pFX-SECFP-Sec61 beta |
Expression vector of SECFP with ER-targeting sequence (human SEC61B) in mammalian cells. |
RDB18312 |
pER-mAG1 |
CoralHue™ ER-targeted monomeric Azami-Green 1 |
RDB18326 |
pER-mAG407 |
CoralHue™ ER-targeted monomeric Azami-Green407 |
RDB14673 |
304-SEC71TMD-GFP |
Expression vector of S. cerevisiae SEC71TMD (Sec71_transmembrane domain), fused with GFP for labeling ER. |
RDB19613 |
er-(n2)oxStayGold(c4) |
Vector for ER labeling with fluorescent protein oxStayGold |
RDB18317 |
pER-mKO1 |
CoralHue™ ER-targeted monomeric Kusabira-Orange 1 |
Focal adhesions
- Nano-lantern clones
Catalog no. |
Name of clone |
Descriptiion |
RDB13342 |
pCNL-N1_zyxin |
Cyan Nano-lantern for labeling of focal adhesions by the mouse zyxin. |
RDB13341 |
pYNL-N1_zyxin |
Yellow Nano-lantern for labeling of focal adhesions by the mouse zyxin. |
RDB13343 |
pONL-N1_zyxin |
Orange Nano-lantern for labeling of focal adhesions by the mouse zyxin. |
Golgi apparatus
- Epitope-tagged protein probe
Catalog no. |
Name of clone |
Descriptiion |
RDB03423 |
FLAG-Rab27A |
Expression vector of mouse Rab27A cDNA which can be used as secretory granule and melanosomme markers. |
- Fluorescent protein porbe
Catalog no. |
Name of clone |
Descriptiion |
RDB19412 |
pFX-GalT-SECFP |
Expression vector of SECFP with Golgi-targeting sequence (human B4GALT1) in mammalian cells. |
RDB20222 |
pcDNA3/td5StayGold(c4)=GianCreg |
Expression vector of td5StayGold for labeling the Golgi apparatus. |
RDB15379 |
pEGFP-C1-mouse Rab33B |
Expression vector of mouse RAB33B, N-terminal EGFP-tag. |
RDB14670 |
316-GNT1-GFP |
Expression vector of S. cerevisiae GNT1, fused with GFP for labeling medial cisternae. |
RDB14671 |
314-GNT1-GFP |
Expression vector of S. cerevisiae GNT1, fused with GFP for labeling medial cisternae. |
RDB14672 |
316-SYS1-GFP |
Expression vector of S. cerevisiae SYS1, fused with GFP for labeling trans cisternae. |
RDB08663 |
316-SEC7-mRFP |
Plasmid clone of trans-Golgi marker SEC7-mRFP in S. cerevisiae |
- Nano-lantern clones
Catalog no. |
Name of clone |
Descriptiion |
RDB13354 |
pCNL-N1_Golgi |
Cyan Nano-lantern for labeling of Golgi apparatus by the N-terminal 81 amino acids of human beta 1,4-galactosyltransferase. |
RDB13353 |
pYNL-N1_Golgi |
Yellow Nano-lantern for labeling of Golgi apparatus by the N-terminal 81 amino acids of human beta 1,4-galactosyltransferase. |
RDB13355 |
pONL-N1_Golgi |
Orange Nano-lantern for labeling of Golgi apparatus by the N-terminal 81 amino acids of human beta 1,4-galactosyltransferase. |
Kinetochore
- Fluorescent protein porbe
Catalog no. |
Name of clone |
Descriptiion |
RDB20285 |
pGEMHE-mHec1-sfGFP |
Labeling outer kinetochore. Plasmid for in vitro transcription of mouse Hec1 (Ndc80) in mouse oocyte. |
RDB13518 |
pGEMHE-tdTomato-mCENP-C |
Labeling inner kinetochore. Plasmid for in vitro transcription of mouse CENP-C in mouse oocyte. |
Lysosome
- Nano-lantern clones
Catalog no. |
Name of clone |
Descriptiion |
RDB13345 |
pCNL-N1_LAMP1 |
Cyan Nano-lantern for labeling of lysosome by the rat LAMP1. |
RDB13344 |
pYNL-N1_LAMP1 |
Yellow Nano-lantern for labeling of lysosome by the rat LAMP1. |
RDB13346 |
pONL-N1_LAMP1 |
Orange Nano-lantern for labeling of lysosome by the rat LAMP1. |
- Related article
Melanosome
- Epitope-tagged protein probe
Catalog no. |
Name of clone |
Descriptiion |
RDB18649 |
pEF-HA-M-INK |
M-INK, a tool for tagging mature melanosomes. |
RDB17956 |
pMRX-bsr-mCherry-M-INK |
M-INK, a tool for tagging mature melanosomes. Retroviral expression vector of mouse Kif1c tagged with mCherry. |
RDB17955 |
pMRX-bsr-Myc-M-INK |
M-INK, a tool for tagging mature melanosomes. Retroviral expression vector of mouse Kif1c tagged with Myc. |
RDB14634 |
pEF-T7-GST-Kif1c-tail-879-1100/M-INK |
M-INK, a novel tool for tagging the mature melanosomes. Expression vector of T7- and GST- tagged C terminal half tail region of the mouse Kif1c. |
RDB03423 |
FLAG-Rab27A |
Expression vector of mouse Rab27A cDNA which can be used as secretory granule and melanosomme markers. |
- Fluorescent protein porbe
- Related article
Mitochondria
- Epitope-tagged protein probe
Catalog no. |
Name of clone |
Descriptiion |
RDB17747 |
pN1-OPA1(123Nt)-Halo |
Halo Tag, Mitochondrial intermembrane space localization |
RDB17750 |
pN1-TOMM20-Halo |
Halo Tag, Mitochondrial outer membrane localization |
- Fluorescent protein probe
Catalog no. |
Name of clone |
Descriptiion |
RDB18323 |
pMT-mMiCy1 |
CoralHue™ Mitochondria-targeted monomeric Midoriishi-Cyan 1 |
RDB19410 |
pFX-Tom20-SECFP |
Expression vector of SECFP with mitochondria-targeting sequence (human TOMM20) in mammalian cells. |
RDB18311 |
pMT-mAG1 |
CoralHue™ Mitochondria-targeted monomeric Azami-Green |
RDB19415 |
pFX-mito-EGFP |
Expression vector of EGFP with mitochondria-targeting sequence (human COX8A) in mammalian cells. |
RDB19614 |
mt-(n1)StayGold |
Vector for Mitochondria labeling with fluorescent protein StayGold |
RDB20228 |
pcDNA3/Cox8a=mStayGold |
Expression vector of mStayGold as a fluorescent marker of the inner mitochondrial membrane. |
RDB18316 |
pMT-mKO1 |
CoralHue™ Mitochondria-targeted monomeric Kusabira-Orange 1 |
RDB15238 |
mt-mKeima (h)/pIND(SP1) |
Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal. |
RDB15456 |
mt-mKeima/pcDNA3 |
Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal. |
- Nano-lantern clones
Catalog no. |
Name of clone |
Descriptiion |
RDB13330 |
pCNL-N1_Mito |
Cyan Nano-lantern for labeling of mitochondria by the mitochondrial targeting sequence from subunit VIII of human cytochrome c oxdase. |
RDB13329 |
pYNL-N1_Mito |
Yellow Nano-lantern for labeling of mitochondria by the mitochondrial targeting sequence from subunit VIII of human cytochrome c oxdase. |
RDB13331 |
pONL-N1_Mito |
Orange Nano-lantern for labeling of mitochondria by the mitochondrial targeting sequence from subunit VIII of human cytochrome c oxdase. |
Nuclei
- Fluorescent protein probe
Catalog no. |
Name of clone |
Descriptiion |
RDB15740 |
GFP-NLS SV40 |
Fluorescent nuclear marker accumulated not only in the nucleoplasm but also in the nucleolus. |
RDB15741 |
GFP-NLS PARP |
Fluorescent nuclear marker, broader distribution in nucleus and leaky in cytoplasm. |
- Nano-lantern clones
Catalog no. |
Name of clone |
Descriptiion |
RDB13333 |
pCNL-N1_3xNLS |
Cyan Nano-lantern for labeling of nuclei by the nuclear localization signal of SV40. |
RDB13332 |
pYNL-N1_3xNLS |
Yellow Nano-lantern for labeling of nuclei by the nuclear localization signal of SV40. |
RDB13334 |
pONL-N1_3xNLS |
Orange Nano-lantern for labeling of nuclei by the nuclear localization signal of SV40. |
Nuclei in interphase
- Fluorescent protein probe
Catalog no. |
Name of clone |
Descriptiion |
RDB19414 |
pFX-H2B-SECFP |
Expression vector of SECFP with nucleus-targeting sequence (human H2B) in mammalian cells. |
- Nano-lantern clones
Catalog no. |
Name of clone |
Descriptiion |
RDB13363 |
pCNL-C1_H2B |
Cyan Nano-lantern for labeling of nuclei in interphase by the human histone H2B. |
RDB13362 |
pYNL-C1_H2B |
Yellow Nano-lantern for labeling of nuclei in interphase by the human histone H2B. |
RDB13364 |
pONL-C1_H2B |
Orange Nano-lantern for labeling of nuclei in interphase by the human histone H2B. |
Nucleoli
- Fluorescent protein probe
Catalog no. |
Name of clone |
Descriptiion |
RDB15739 |
mCherry-fibrillarin |
Fluorescent nucleolus marker. |
- Nano-lantern clones
Catalog no. |
Name of clone |
Descriptiion |
RDB13369 |
pCNL-C1_fibrillarin |
Cyan Nano-lantern for labeling of nucleoli by the human fibrillarin. |
RDB13368 |
pYNL-C1_fibrillarin |
Yellow Nano-lantern for labeling of nucleoli by the human fibrillarin. |
RDB13370 |
pONL-C1_fibrillarin |
Orange Nano-lantern for labeling of nucleoli by the human fibrillarin. |
Nucleoplasm
- Fluorescent protein probe
Catalog no. |
Name of clone |
Descriptiion |
RDB18325 |
pNP-hKCy1 |
CoralHue™ Nucleoplasm-targeted humanized Kusabira-Cyan 1 |
RDB15746 |
GFP-NLS NP |
Fluorescent nuclear marker. |
RDB18315 |
pNP-AG |
CoralHue™ Nucleoplasm-targeted Azami-Green |
RDB18320 |
pNP-KO1 |
CoralHue™ Nucleoplasm-targeted Kusabira-Orange 1 |
RDB18327 |
pNP-hdKeima570 |
CoralHue™ Nucleoplasm-targeted humanized dimeric Keima570 |
RDB18324 |
pNP-hdKeima-Red |
CoralHue™ Nucleoplasm-targeted humanized dimeric Keima-Red |
Peroxisome
- Nano-lantern clones
Catalog no. |
Name of clone |
Descriptiion |
RDB13360 |
pCNL-C1_PTS1 |
Cyan Nano-lantern for labeling of peroxisome by the peroxisomal targeting signal 1 (tripeptide SKL). |
RDB13336 |
pCNL-N1_PTS2 |
Cyan Nano-lantern for labeling of peroxisome by the peroxisomal targeting signal 2. |
RDB13359 |
pYNL-C1_PTS1 |
Yellow Nano-lantern for labeling of peroxisome by the peroxisomal targeting signal 1 (tripeptide SKL). |
RDB13335 |
pYNL-N1_PTS2 |
Yellow Nano-lantern for labeling of peroxisome by the peroxisomal targeting signal 2. |
RDB13361 |
pONL-C1_PTS1 |
Orange Nano-lantern for labeling of peroxisome by the peroxisomal targeting signal 1 (tripeptide SKL). |
RDB13337 |
pONL-N1_PTS2 |
Orange Nano-lantern for labeling of peroxisome by the peroxisomal targeting signal 2. |
Plasma membrane and endosome
- Epitope-tagged protein probe
Catalog no. |
Name of clone |
Descriptiion |
RDB14677 |
pEF-BOS FLAG-mouse Rab5A |
Expression vector of mouse Rab5A, which is localized in early endosome. |
RDB14678 |
pEF-BOS FLAG-mouse Rab7 |
Expression vector of mouse Rab7, which is localized in late endosome. |
- Fluorescent protein probe
Catalog no. |
Name of clone |
Descriptiion |
RDB19416 |
pFX-EGFP-EEA1 |
Expression vector of EGFP with early endosome-targeting sequence (human EEA1) in mammalian cells. |
RDB19417 |
pFX-EGFP-Rab5 |
Expression vector of EGFP with early endosome-targeting sequence (mouse RAB5A) in mammalian cells. |
RDB19418 |
pFX-EGFP-Rab7 |
Expression vector of EGFP with late endosome-targeting sequence (mouse RAB7A) in mammalian cells. |
RDB19419 |
pFX-EGFP-Rab11 |
Expression vector of EGFP with recycling endosome-targeting sequence (human RAB11A) in mammalian cells. |
RDB19214 |
pET28/His6-EGFP-Lact-C2 |
Expression vector of His and EGFP-tagged C2 domain of bovine Lactadherin for E. coli. Phosphatidylserine (PS)-specific probe. |
RDB18313 |
pPM-mAG1 |
CoralHue™ Plasma Membrane-targeted monomeric Azami-Green 1. |
RDB18318 |
pPM-mKO1 |
CoralHue™ Plasma Membrane-targeted mKusabira-Orange 1. |
RDB18322 |
pPM-mKeima-Red |
CoralHue™ Plasma Membrane-targeted mKeima-Red. |
RDB15030 |
pmStr-C1-mouse Rab11A |
Expression vector of mouse Rab11A, Recycling endosome marker. |
RDB13501 |
pCAG/tdiRFP-CAAX |
Marker of plasma membrane. Mammalian expression plasmid of CAAX box protein. |
- Nano-lantern clones
Catalog no. |
Name of clone |
Descriptiion |
RDB13357 |
pCNL-C1_RhoB |
Cyan Nano-lantern for labeling of plasma membrane and endosomes by the human RhoB. |
RDB13366 |
pCNL-C1_Rab11a |
Cyan Nano-lantern for labeling of recycling endosomes by the mouse Rab11a. |
RDB13356 |
pYNL-C1_RhoB |
Yellow Nano-lantern for labeling of plasma membrane and endosomes by the human RhoB. |
RDB13365 |
pYNL-C1_Rab11a |
Yellow Nano-lantern for labeling of recycling endosomes by the mouse Rab11a. |
RDB13358 |
pONL-C1_RhoB |
Orange Nano-lantern for labeling of plasma membrane and endosomes by the human RhoB. |
RDB13367 |
pONL-C1_Rab11a |
Orange Nano-lantern for labeling of recycling endosomes by the mouse Rab11a. |
- Related article
Sphingolipid marker
- Lipid rafts are small lipid domains on the cell membrane and are thought to play an important role in signal transduction, endocytosis and more. We provide fluorescent probes for sphingomyelin and cholesterol lipid domains.
- Nakanori: sphingomyelin and cholesterol lipid domain (lipid raft)
Catalog no. |
Name of clone |
Descriptiion |
RDB14301 |
pET28/His6-EGFP-Nakanori |
Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of His6-EGFP-tagged G. frondosa Nakaniori. |
- D4 toxin: cholesterol rich domain
Catalog no. |
Name of clone |
Descriptiion |
RDB13961 |
pET28/His6-EGFP-D4 |
Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of His6-EGFP-tagged perfringolysin theta toxin D4 of C. perfringens. |
RDB14300 |
pET28/His6-mCherry-D4 |
Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of His6-mCherry-tagged perfringolysin theta toxin D4 of C. perfringens. |
RDB15469 |
pET28/Dronpa-theta-D4 |
Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of perfringolysin theta toxin D4 of C. perfringens. |
- lysenin: sphingomyelin
Catalog no. |
Name of clone |
Descriptiion |
RDB13498 |
pET28/His6-EGFP-NT-Lys |
Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin. |
RDB13499 |
pET28/His6-mCherry-NT-Lys |
Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin. |
RDB13958 |
pMAL/MBP-lysenin |
Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of MBP-tagged lysenin. |
RDB13959 |
pQE30/His6-Venus-NT-Lys |
Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of His6-Venus-tagged non toxic lysenin. |
RDB13960 |
pQE30/His6-mRFP-NT-Lys |
Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of His6-mRFP-tagged non toxic lysenin. |
RDB15470 |
pET28/Dronpa-NT-Lys |
Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin. |
RDB19208 |
pHalo-NT-Lys |
Mammalian expression vector of HaloTag(R) 7-tagged nontoxic Lysenin. |
RDB19215 |
pET28/His6-NT-Lys |
Expression vector of His-tagged nontoxic Lysenin for E. coli. |
- Other markers
Catalog no. |
Name of clone |
Descriptiion |
RDB13496 |
pET28/EqtII-EGFP-His6 |
Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of Eqinatoxin II. |
RDB13497 |
pET28/EqtII(8-69)-EGFP-His6 |
Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Eqinatoxin II. |
RDB13962 |
pET28/PlyA2-EGFP-His6 |
Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged PlyA2. |
RDB13963 |
pET28/Oly-EGFP-His6 |
Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged Oly. |
RDB13964 |
pET28/EryA-EGFP-His6 |
Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged EryA. |
- Related article
Tubulin (β-tubulin)
- Fluorescent protein probe
Catalog no. |
Name of clone |
Descriptiion |
RDB19413 |
pFX-beta-tubulin-SECFP |
Expression vector of SECFP with microtuble-targeting sequence (human TUBB4B) in mammalian cells. |
- Nano-lantern clones
Catalog no. |
Name of clone |
Descriptiion |
RDB13339 |
pCNL-N1_TUBB5 |
Cyan Nano-lantern for labeling of beta-tubulin by the mouse beta-tubulin class V. |
RDB13338 |
pYNL-N1_TUBB5 |
Yellow Nano-lantern for labeling of beta-tubulin by the mouse beta-tubulin class V. |
RDB13340 |
pONL-N1_TUBB5 |
Orange Nano-lantern for labeling of beta-tubulin by the mouse beta-tubulin class V. |
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Visualizing multiple inter-organelle contact sites
Evaluating intracellular organelle (mitochondria-associated membrane)
- Novel reporters of mitochondria-associated membranes (MAM), MAMtrackers, demonstrate MAM disruption as a common pathological feature in amyotrophic lateral sclerosis.
Sakai, S., Watanabe, S., Komine, O., Sobue, A., Yamanaka, K.
FASEB J. 35 (7): e21688 (2021). PubMed PMID 34143516.
Catalog no. |
Name of clone |
Descriptiion |
RDB19895 |
pMAMtracker-Luc |
A reporter plasmid based on split-Luciferase for evaluating intracellular organelle (mitochondria-associated membrane). |
RDB19896 |
pMAMtracker-Green |
A reporter plasmid based on split-GFP for evaluating intracellular organelle (mitochondria-associated membrane). |
Fluorescent protein probe that can visualize the multiple inter-organelle contact sites in cells.
- Divided GFP-fragment have a feature that they emit fluorescence again by reconstitution when each fragment is in close proximity. When labelled organelles such as mitochondria and ERs by the divided GFPs exist together, the contact sites in cells are visualized by the reconstituted fluorescent protein. The vectors of fluorescent protein probes developed by Dr. Yasushi Tamura of the Yamagata University, Dr. Toshiya Endo of the Kyoto Sangyo University and their colleague are available from the DNA Bank.
- Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system.
Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y.
Sci. Rep. 8 (1): 6175 (2018). PubMed PMID 29670150.
- Protrudin and PDZD8 contribute to neuronal integrity by promoting lipid extraction required for endosome maturation.
Shirane, M., Wada, M., Morita, K., Hayashi, N., Kunimatsu, R., Matsumoto, Y., Matsuzaki, F., Nakatsumi, H., Ohta, K., Tamura, Y., Nakayama, K.I.
Nat. Commun. 11 (1): 4576 (2020). PubMed PMID 32917905.
- Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells.
Tashiro, S., Kakimoto, Y., Shinmyo, M., Fujimoto, S., Tamura, Y.
Front. Cell Dev. Biol. 8: 571388 (2020). PubMed PMID 33330450.
Split-GFP probe plasmid usable in cultured cells
First ten beta-strands (GFP1-10)
Catalog # |
Name of resource 1 |
Name of reource 2 |
Localization |
RDB16041 |
pMM80 |
pcDNA3.1_Tom20N-3xFLAG-GFP1-10 |
mitochondria |
RDB16042 |
pMM82 |
pcDNA3.1-Tom70N-3xFLAG-GFP1-10 |
mitochondria |
RDB18663 |
pMM86 |
pcDNA3.1-ERj1N-V5-GFP1-10 |
endoplasmic reticulum |
Last 11th beta-strand (GFP11)
Catalog # |
Name of resource 1 |
Name of reource 2 |
Localization |
RDB18662 |
pMM83 |
pcDNA3.1-Tom70N-3xFLAG-GFP11 |
mitochondria |
RDB18665 |
pMM189 |
pIRESNeo3-TOMM70 1-70-3xFLAG-GFP11 |
mitochondria |
RDB16047 |
pMM87 |
pcDNA3.1_ERj1N-V5-GFP11 |
endoplasmic reticulum |
RDB16048 |
pMM89 |
pcDNA3.1_Sec63N-V5-GFP11 |
endoplasmic reticulum |
RDB18655 |
pMM104 |
pcDNA3.1_Lamp1-HA_GFP11 |
lysosome |
Split-GFP probe plasmid usable in budding yeast (for genome integration)
First ten beta-strands (GFP1-10)
Catalog # |
Name of resource 1 |
Name of reource 2 |
Localization |
DNA size (bp) |
RDB18659 |
pYC141 |
pBS-GPDp-Ifa38-GFP1-10-CyC1ter-hphMX |
endoplasmic reticulum |
ca. 4,500 |
RDB18660 |
pYC143 |
pBS-GPDp-Tom71-GFP1-10-CyC1ter-hphMX |
mitochondria |
ca. 5,300 |
RDB18661 |
pYC144 |
pBS-GPDp-Pex3N-GFP1-10-CyC1ter-hphMX |
peroxisome |
ca. 3,600 |
Last 11th beta-strand (GFP11)
Catalog # |
Name of resource 1 |
Name of reource 2 |
Localization |
DNA size (bp) |
RDB18656 |
pYC135 |
pBS-GPDp-Ifa38-V5-GFP11-CyC1ter-natNT2 |
endoplasmic reticulum |
ca. 3,700 |
RDB18657 |
pYC136 |
pBS-GPDp-Erg6-V5-GFP11-CyC1ter-natNT2 |
lipid droplets |
ca. 3,800 |
RDB18658 |
pYC137 |
pBS-GPDp-DPP1-V5-GFP11-CyC1ter-natNT2 |
vacuole |
ca. 3,600 |
Split-GFP probe plasmid usable in budding yeast (for plasmid expressionn)
First ten beta-strands (GFP1-10)
Catalog # |
Name of resource 1 |
Name of reource 2 |
Localization |
Marker gene |
RDB16037 |
pSFL22 |
pRS316-GPDp-Tom71-GFP1-10 |
mitochondria |
URA3 |
RDB16043 |
pSFL16 |
pRS316-GPDp-Ifa38-GFP1-10 |
endoplasmic reticulum |
URA3 |
RDB16049 |
pSFL19 |
pRS316-GPDp-Vph1-GFP1-10 |
vacuole |
URA3 |
RDB16051 |
pSFL26 |
pRS316-GPDp-Pex3N-GFP1-10 |
peroxisome |
URA3 |
RDB16053 |
pSFL28 |
pRS316-GPDp-Erg6-GFP1-10 |
lipid droplets |
URA3 |
RDB18571 |
pSFL17 |
pRS315-GPDp-Ifa38-GFP1-10 |
endoplasmic reticulum |
LEU2 |
Last 11th beta-strand (GFP11)
Catalog # |
Name of resource 1 |
Name of reource 2 |
Localization |
Marker gene |
RDB16038 |
pSFL23 |
pRS314-GPDp-Tom71-GFP11 |
mitochondria |
TRP1 |
RDB16039 |
pSFL75 |
pRS314-GPDp-Tom71-V5-GFP11 |
mitochondria |
TRP1 |
RDB16040 |
pSFL15 |
pRS314-GPDp-YNR021W-GFP11 |
endoplasmic reticulum |
TRP1 |
RDB16044 |
pSFL18 |
pRS314-GPDp-Ifa38-GFP11 |
endoplasmic reticulum |
TRP1 |
RDB16045 |
pSFL86 |
pRS313-GPDp-Ifa38-3xFLAG-GFP11 |
endoplasmic reticulum |
HIS3 |
RDB16046 |
pSFL59 |
pRS314-GPDp-Ifa38-mCherry-GFP11 |
endoplasmic reticulum |
TRP1 |
RDB16050 |
pSFL108 |
pRS314-GPDp-Dpp1-V5-GFP11 |
vacuole |
TRP1 |
RDB16052 |
pSFL96 |
pRS316-GPDp-Pex3N-3xFLAG-GFP11 |
peroxisome |
URA3 |
RDB16054 |
pSFL67 |
pRS314-GPDp-Erg6-3xFLAG-GFP11 |
lipid droplets |
TRP1 |
RDB18572 |
pSFL61 |
pRS316-GPDp-Ifa38-mCherry-GFP11 |
endoplasmic reticulum |
URA3 |
RDB18573 |
pSFL66 |
pRS314-GPDp-Ifa38-3xFLAG-GFP11 |
endoplasmic reticulum |
TRP1 |
RDB18574 |
pSFL68 |
pRS314-GPDp-Erg6-V5-GFP11 |
lipid droplets |
TRP1 |
RDB18577 |
pSFL76 |
pRS314-GPDp-Ifa38-V5-GFP11 |
endoplasmic reticulum |
TRP1 |
Backborn constructs
First ten beta-strands (GFP1-10)
Last 11th beta-strand (GFP11)
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GPI anchored Reconstitution-Activated Proteins Highlight Intercellular Connections (GRAPHIC)
- Genetically Encoded Fluorescent Indicator GRAPHIC Delineates Intercellular Connections.
Kinoshita, N., Huang, A.J.Y., McHugh, T.J., Suzuki, S.C., Masai, I., Kim, I.H., Soderling, S.H., Miyawaki, A., Shimogori, T.
iScience 15: 28-38 (2019). PubMed PMID 31026667.
- Diffusible GRAPHIC to visualize morphology of cells after specific cell-cell contact.
Kinoshita, N., Huang, A.J.Y., McHugh, T.J., Miyawaki, A., Shimogori, T.
Sci. Rep. 10 (1): 14437 (2020). PubMed PMID 32879377.
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FPs with tissue specific promoter
Synapsin I (SYN) promoter
Catalog no. |
Name of clone |
Descriptiion |
RDB15107 |
pBSIISK-SYN-GFP-BGHpA |
Expression vector of GFP driven by the neuron-specific promoter (human synapsin I (SYN)). |
RDB15109 |
pENTR1A-SYN-GFP-BGHpA(r) |
Gateway(R) entry vector of GFP expression unit driven by the neuron-specific promoter (human synapsin I (SYN)). |
RDB15113 |
pAAV2-SYN-GFP-BGHpA |
AAV2 expression vector of GFP driven by the neuron-specific promoter (human synapsin I (SYN)). |
Broad expression
Catalog no. |
Name of clone |
Descriptiion |
RDB06071 |
pCMFlag_EGFP |
Expression vector of GFP, with FLAG-tag, control vector |
RDB15108 |
pENTR1A-CMV-GFP-BGHpA(r) |
Gateway(R) entry vector of GFP expression unit driven by the ubiquious promoter (CMV) |
RDB15112 |
pAAV2-CMV-GFP-BGHpA |
AAV2 expression vector of GFP driven by the ubiquious promoter (CMV) |
(GRP0053e 2018.04.24 T.M.)
2024.12.04