Visualization of Organelles
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Organelle marker
Visualizing multiple inter-organelle contact sites
GPI anchored Reconstitution-Activated Proteins Highlight Intercellular Connections (GRAPHIC)
FPs with tissue specific promoter
Organelle marker
We are providing genetic resources for visualization of organelles such as mitochondria and nucleus. Each clone contains an organelle localization signal sequence fused with fluorescent proteins or epitope tags. Organelles can be detected by fluorescence or by detecting epitope tags with antibodies.
Of those, 12 species of organelles can be observed by Nano-lantern luminescent and fluorescent proteins developed by Drs. Okada and Takai of the RIKEN Center for Biosystems Dynamics Research (BDR) and Nagai and their colleague of the Osaka University [Takai, A. et al., Proc Natl Acad Sci U S A. 112 (14): 4352-4356, 2015]. Nano-lantern is a brighter luminescent protein, consisting of luciferase and fluorescent protein. Three colors (yellow, cyan and orange) of Nano-lanterns are available for each localization signal.
Actin (β-actin)
- Fluorescent protein porbe
Catalog no. Name of clone Running title RDB18314 pActin-mAG1 CoralHue™ β-Actin-targeted monomeric Azami-Green 1 RDB18319 pActin-hmKO1 CoralHue™ β-Actin-targeted monomeric Kusabira-Orange 1
Actin (F-actin)
- Nano-lantern clones
Catalog no. Name of clone Running title RDB13348 pCNL-N1_ITPKA Cyan Nano-lantern for labeling of F-actin by the rat inositol triphosphate 3-kinase A. RDB13347 pYNL-N1_ITPKA Yellow Nano-lantern for labeling of F-actin by the rat inositol triphosphate 3-kinase A. RDB13349 pONL-N1_ITPKA Orange Nano-lantern for labeling of F-actin by the rat inositol triphosphate 3-kinase A.
Cytoskeleton and focal adhesion
- Nano-lantern clones
Catalog no. Name of clone Running title RDB13351 pCNL-N1_vinculin Cyan Nano-lantern with the chicken vinculin. RDB13350 pYNL-N1_vinculin Yellow Nano-lantern with the chicken vinculin. RDB13352 pONL-N1_vinculin Orange Nano-lantern with the chicken vinculin.
Dense-core vesicles
- Fluorescent protein porbe
Catalog no. Name of clone Running title RDB15118 pNPY-Venus-N1 Venus fluorescent protein targeting localization in neurites. Mammalian expression. RDB15119 NPY-Venus/pEF-BOS Venus fluorescent protein targeting localization in neurites. Mammalian expression.
Endoplasmic reticulum
- Fluorescent protein porbe
Catalog no. Name of clone Running title RDB19411 pFX-SECFP-Sec61 beta Expression vector of SECFP with ER-targeting sequence (human SEC61B) in mammalian cells. RDB18312 pER-mAG1 CoralHue™ ER-targeted monomeric Azami-Green 1 RDB18326 pER-mAG407 CoralHue™ ER-targeted monomeric Azami-Green407 RDB14673 304-SEC71TMD-GFP Expression vector of S. cerevisiae SEC71TMD (Sec71_transmembrane domain), fused with GFP for labeling ER. RDB19613 er-(n2)oxStayGold(c4) Vector for ER labeling with fluorescent protein oxStayGold RDB18317 pER-mKO1 CoralHue™ ER-targeted monomeric Kusabira-Orange 1
Focal adhesions
- Nano-lantern clones
Catalog no. Name of clone Running title RDB13342 pCNL-N1_zyxin Cyan Nano-lantern for labeling of focal adhesions by the mouse zyxin. RDB13341 pYNL-N1_zyxin Yellow Nano-lantern for labeling of focal adhesions by the mouse zyxin. RDB13343 pONL-N1_zyxin Orange Nano-lantern for labeling of focal adhesions by the mouse zyxin.
Golgi apparatus
- Epitope-tagged protein probe
Catalog no. Name of clone Running title RDB03423 FLAG-Rab27A Expression vector of mouse Rab27A cDNA which can be used as secretory granule and melanosomme markers. - Fluorescent protein porbe
Catalog no. Name of clone Running title RDB19412 pFX-GalT-SECFP Expression vector of SECFP with Golgi-targeting sequence (human B4GALT1) in mammalian cells. RDB15379 pEGFP-C1-mouse Rab33B Expression vector of mouse RAB33B, N-terminal EGFP-tag. RDB14670 316-GNT1-GFP Expression vector of S. cerevisiae GNT1, fused with GFP for labeling medial cisternae. RDB14671 314-GNT1-GFP Expression vector of S. cerevisiae GNT1, fused with GFP for labeling medial cisternae. RDB14672 316-SYS1-GFP Expression vector of S. cerevisiae SYS1, fused with GFP for labeling trans cisternae. RDB08663 316-SEC7-mRFP Plasmid clone of trans-Golgi marker SEC7-mRFP in S. cerevisiae - Nano-lantern clones
Catalog no. Name of clone Running title RDB13354 pCNL-N1_Golgi Cyan Nano-lantern for labeling of Golgi apparatus by the N-terminal 81 amino acids of human beta 1,4-galactosyltransferase. RDB13353 pYNL-N1_Golgi Yellow Nano-lantern for labeling of Golgi apparatus by the N-terminal 81 amino acids of human beta 1,4-galactosyltransferase. RDB13355 pONL-N1_Golgi Orange Nano-lantern for labeling of Golgi apparatus by the N-terminal 81 amino acids of human beta 1,4-galactosyltransferase.
Kinetochore
- Fluorescent protein porbe
Catalog no. Name of clone Running title RDB13516 pGEMHE-mHec1-sfGFP Labeling outer kinetochore. Plasmid for in vitro transcription of mouse Hec1 (Ndc80) in mouse oocyte. RDB13518 pGEMHE-tdTomato-mCENP-C Labeling inner kinetochore. Plasmid for in vitro transcription of mouse CENP-C in mouse oocyte.
Lysosome
- Nano-lantern clones
Catalog no. Name of clone Running title RDB13345 pCNL-N1_LAMP1 Cyan Nano-lantern for labeling of lysosome by the rat LAMP1. RDB13344 pYNL-N1_LAMP1 Yellow Nano-lantern for labeling of lysosome by the rat LAMP1. RDB13346 pONL-N1_LAMP1 Orange Nano-lantern for labeling of lysosome by the rat LAMP1. - Related article
Melanosome
- Epitope-tagged protein probe
Catalog no. Name of clone Running title RDB18649 pEF-HA-M-INK M-INK, a tool for tagging mature melanosomes. RDB17956 pMRX-bsr-mCherry-M-INK M-INK, a tool for tagging mature melanosomes. Retroviral expression vector of mouse Kif1c tagged with mCherry. RDB17955 pMRX-bsr-Myc-M-INK M-INK, a tool for tagging mature melanosomes. Retroviral expression vector of mouse Kif1c tagged with Myc. RDB14634 pEF-T7-GST-Kif1c-tail-879-1100/M-INK M-INK, a novel tool for tagging the mature melanosomes. Expression vector of T7- and GST- tagged C terminal half tail region of the mouse Kif1c. RDB03423 FLAG-Rab27A Expression vector of mouse Rab27A cDNA which can be used as secretory granule and melanosomme markers. - Fluorescent protein porbe
Catalog no. Name of clone Running title RDB15184 pCMV-Mreg delta C/MST-mStr MST tag, a novel melanosome-targeting tag. - Related article
Mitochondria
- Epitope-tagged protein probe
Catalog no. Name of clone Running title RDB17747 pN1-OPA1(123Nt)-Halo Halo Tag, Mitochondrial intermembrane space localization RDB17750 pN1-TOMM20-Halo Halo Tag, Mitochondrial outer membrane localization - Fluorescent protein probe
Catalog no. Name of clone Running title RDB18323 pMT-mMiCy1 CoralHue™ Mitochondria-targeted monomeric Midoriishi-Cyan 1 RDB19410 pFX-Tom20-SECFP Expression vector of SECFP with mitochondria-targeting sequence (human TOMM20) in mammalian cells. RDB18311 pMT-mAG1 CoralHue™ Mitochondria-targeted monomeric Azami-Green RDB19415 pFX-mito-EGFP Expression vector of EGFP with mitochondria-targeting sequence (human COX8A) in mammalian cells. RDB19614 mt-(n1)StayGold Vector for Mitochondria labeling with fluorescent protein StayGold RDB18316 pMT-mKO1 CoralHue™ Mitochondria-targeted monomeric Kusabira-Orange 1 RDB18321 pMT-mKeima-Red CoralHue™ Mitochondria-targeted mKeima-Red RDB15238 mt-mKeima (h)/pIND(SP1) Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal. RDB15456 mt-mKeima/pcDNA3 Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal. - Nano-lantern clones
Catalog no. Name of clone Running title RDB13330 pCNL-N1_Mito Cyan Nano-lantern for labeling of mitochondria by the mitochondrial targeting sequence from subunit VIII of human cytochrome c oxdase. RDB13329 pYNL-N1_Mito Yellow Nano-lantern for labeling of mitochondria by the mitochondrial targeting sequence from subunit VIII of human cytochrome c oxdase. RDB13331 pONL-N1_Mito Orange Nano-lantern for labeling of mitochondria by the mitochondrial targeting sequence from subunit VIII of human cytochrome c oxdase.
Nuclei
- Fluorescent protein probe
Catalog no. Name of clone Running title RDB15740 GFP-NLS SV40 Fluorescent nuclear marker accumulated not only in the nucleoplasm but also in the nucleolus. RDB15741 GFP-NLS PARP Fluorescent nuclear marker, broader distribution in nucleus and leaky in cytoplasm. - Nano-lantern clones
Catalog no. Name of clone Running title RDB13333 pCNL-N1_3xNLS Cyan Nano-lantern for labeling of nuclei by the nuclear localization signal of SV40. RDB13332 pYNL-N1_3xNLS Yellow Nano-lantern for labeling of nuclei by the nuclear localization signal of SV40. RDB13334 pONL-N1_3xNLS Orange Nano-lantern for labeling of nuclei by the nuclear localization signal of SV40.
Nuclei in interphase
- Fluorescent protein probe
Catalog no. Name of clone Running title RDB19414 pFX-H2B-SECFP Expression vector of SECFP with nucleus-targeting sequence (human H2B) in mammalian cells. - Nano-lantern clones
Catalog no. Name of clone Running title RDB13363 pCNL-C1_H2B Cyan Nano-lantern for labeling of nuclei in interphase by the human histone H2B. RDB13362 pYNL-C1_H2B Yellow Nano-lantern for labeling of nuclei in interphase by the human histone H2B. RDB13364 pONL-C1_H2B Orange Nano-lantern for labeling of nuclei in interphase by the human histone H2B.
Nucleoli
- Fluorescent protein probe
Catalog no. Name of clone Running title RDB15739 mCherry-fibrillarin Fluorescent nucleolus marker. - Nano-lantern clones
Catalog no. Name of clone Running title RDB13369 pCNL-C1_fibrillarin Cyan Nano-lantern for labeling of nucleoli by the human fibrillarin. RDB13368 pYNL-C1_fibrillarin Yellow Nano-lantern for labeling of nucleoli by the human fibrillarin. RDB13370 pONL-C1_fibrillarin Orange Nano-lantern for labeling of nucleoli by the human fibrillarin.
Nucleoplasm
- Fluorescent protein probe
Catalog no. Name of clone Running title RDB18325 pNP-hKCy1 CoralHue™ Nucleoplasm-targeted humanized Kusabira-Cyan 1 RDB15746 GFP-NLS NP Fluorescent nuclear marker. RDB18315 pNP-AG CoralHue™ Nucleoplasm-targeted Azami-Green RDB18320 pNP-KO1 CoralHue™ Nucleoplasm-targeted Kusabira-Orange 1 RDB18327 pNP-hdKeima570 CoralHue™ Nucleoplasm-targeted humanized dimeric Keima570 RDB18324 pNP-hdKeima-Red CoralHue™ Nucleoplasm-targeted humanized dimeric Keima-Red
Peroxisome
- Nano-lantern clones
Catalog no. Name of clone Running title RDB13360 pCNL-C1_PTS1 Cyan Nano-lantern for labeling of peroxisome by the peroxisomal targeting signal 1 (tripeptide SKL). RDB13336 pCNL-N1_PTS2 Cyan Nano-lantern for labeling of peroxisome by the peroxisomal targeting signal 2. RDB13359 pYNL-C1_PTS1 Yellow Nano-lantern for labeling of peroxisome by the peroxisomal targeting signal 1 (tripeptide SKL). RDB13335 pYNL-N1_PTS2 Yellow Nano-lantern for labeling of peroxisome by the peroxisomal targeting signal 2. RDB13361 pONL-C1_PTS1 Orange Nano-lantern for labeling of peroxisome by the peroxisomal targeting signal 1 (tripeptide SKL). RDB13337 pONL-N1_PTS2 Orange Nano-lantern for labeling of peroxisome by the peroxisomal targeting signal 2.
Plasma membrane and endosome
- Epitope-tagged protein probe
Catalog no. Name of clone Running title RDB14677 pEF-BOS FLAG-mouse Rab5A Expression vector of mouse Rab5A, which is localized in early endosome. RDB14678 pEF-BOS FLAG-mouse Rab7 Expression vector of mouse Rab7, which is localized in late endosome. - Fluorescent protein probe
Catalog no. Name of clone Running title RDB19416 pFX-EGFP-EEA1 Expression vector of EGFP with early endosome-targeting sequence (human EEA1) in mammalian cells. RDB19417 pFX-EGFP-Rab5 Expression vector of EGFP with early endosome-targeting sequence (mouse RAB5A) in mammalian cells. RDB19418 pFX-EGFP-Rab7 Expression vector of EGFP with late endosome-targeting sequence (mouse RAB7A) in mammalian cells. RDB19419 pFX-EGFP-Rab11 Expression vector of EGFP with recycling endosome-targeting sequence (human RAB11A) in mammalian cells. RDB19214 pET28/His6-EGFP-Lact-C2 Expression vector of His and EGFP-tagged C2 domain of bovine Lactadherin for E. coli. Phosphatidylserine (PS)-specific probe. RDB18313 pPM-mAG1 CoralHue™ Plasma Membrane-targeted monomeric Azami-Green 1. RDB18318 pPM-mKO1 CoralHue™ Plasma Membrane-targeted mKusabira-Orange 1. RDB18322 pPM-mKeima-Red CoralHue™ Plasma Membrane-targeted mKeima-Red. RDB15030 pmStr-C1-mouse Rab11A Expression vector of mouse Rab11A, Recycling endosome marker. RDB13501 pCAG/tdiRFP-CAAX Marker of plasma membrane. Mammalian expression plasmid of CAAX box protein. - Nano-lantern clones
Catalog no. Name of clone Running title RDB13357 pCNL-C1_RhoB Cyan Nano-lantern for labeling of plasma membrane and endosomes by the human RhoB. RDB13366 pCNL-C1_Rab11a Cyan Nano-lantern for labeling of recycling endosomes by the mouse Rab11a. RDB13356 pYNL-C1_RhoB Yellow Nano-lantern for labeling of plasma membrane and endosomes by the human RhoB. RDB13365 pYNL-C1_Rab11a Yellow Nano-lantern for labeling of recycling endosomes by the mouse Rab11a. RDB13358 pONL-C1_RhoB Orange Nano-lantern for labeling of plasma membrane and endosomes by the human RhoB. RDB13367 pONL-C1_Rab11a Orange Nano-lantern for labeling of recycling endosomes by the mouse Rab11a. - Related article
Sphingolipid marker
- Lipid rafts are small lipid domains on the cell membrane and are thought to play an important role in signal transduction, endocytosis and more. We provide fluorescent probes for sphingomyelin and cholesterol lipid domains.
- Nakanori: sphingomyelin and cholesterol lipid domain (lipid raft)
Catalog no. Name of clone Running title RDB14301 pET28/His6-EGFP-Nakanori Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of His6-EGFP-tagged G. frondosa Nakaniori. - D4 toxin: cholesterol rich domain
Catalog no. Name of clone Running title RDB13961 pET28/His6-EGFP-D4 Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of His6-EGFP-tagged perfringolysin theta toxin D4 of C. perfringens. RDB14300 pET28/His6-mCherry-D4 Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of His6-mCherry-tagged perfringolysin theta toxin D4 of C. perfringens. RDB15469 pET28/Dronpa-theta-D4 Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of perfringolysin theta toxin D4 of C. perfringens. - lysenin: sphingomyelin
Catalog no. Name of clone Running title RDB13498 pET28/His6-EGFP-NT-Lys Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin. RDB13499 pET28/His6-mCherry-NT-Lys Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin. RDB13958 pMAL/MBP-lysenin Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of MBP-tagged lysenin. RDB13959 pQE30/His6-Venus-NT-Lys Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of His6-Venus-tagged non toxic lysenin. RDB13960 pQE30/His6-mRFP-NT-Lys Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of His6-mRFP-tagged non toxic lysenin. RDB15470 pET28/Dronpa-NT-Lys Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin. RDB19208 pHalo-NT-Lys Mammalian expression vector of HaloTag(R) 7-tagged nontoxic Lysenin. RDB19215 pET28/His6-NT-Lys Expression vector of His-tagged nontoxic Lysenin for E. coli. - Other markers
Catalog no. Name of clone Running title RDB13496 pET28/EqtII-EGFP-His6 Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of Eqinatoxin II. RDB13497 pET28/EqtII(8-69)-EGFP-His6 Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Eqinatoxin II. RDB13962 pET28/PlyA2-EGFP-His6 Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged PlyA2. RDB13963 pET28/Oly-EGFP-His6 Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged Oly. RDB13964 pET28/EryA-EGFP-His6 Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged EryA. - Related article
Tubulin (β-tubulin)
- Fluorescent protein probe
Catalog no. Name of clone Running title RDB19413 pFX-beta-tubulin-SECFP Expression vector of SECFP with microtuble-targeting sequence (human TUBB4B) in mammalian cells. - Nano-lantern clones
Catalog no. Name of clone Running title RDB13339 pCNL-N1_TUBB5 Cyan Nano-lantern for labeling of beta-tubulin by the mouse beta-tubulin class V. RDB13338 pYNL-N1_TUBB5 Yellow Nano-lantern for labeling of beta-tubulin by the mouse beta-tubulin class V. RDB13340 pONL-N1_TUBB5 Orange Nano-lantern for labeling of beta-tubulin by the mouse beta-tubulin class V.
Visualizing multiple inter-organelle contact sites
Fluorescent protein probe that can visualize the multiple inter-organelle contact sites in cells.
- Divided GFP-fragment have a feature that they emit fluorescence again by reconstitution when each fragment is in close proximity. When labelled organelles such as mitochondria and ERs by the divided GFPs exist together, the contact sites in cells are visualized by the reconstituted fluorescent protein. The vectors of fluorescent protein probes developed by Dr. Yasushi Tamura of the Yamagata University, Dr. Toshiya Endo of the Kyoto Sangyo University and their colleague are available from the DNA Bank.
- Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system.
Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y.
Sci. Rep. 8 (1): 6175 (2018). PubMed PMID 29670150. - Protrudin and PDZD8 contribute to neuronal integrity by promoting lipid extraction required for endosome maturation.
Shirane, M., Wada, M., Morita, K., Hayashi, N., Kunimatsu, R., Matsumoto, Y., Matsuzaki, F., Nakatsumi, H., Ohta, K., Tamura, Y., Nakayama, K.I.
Nat. Commun. 11 (1): 4576 (2020). PubMed PMID 32917905. - Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells.
Tashiro, S., Kakimoto, Y., Shinmyo, M., Fujimoto, S., Tamura, Y.
Front. Cell Dev. Biol. 8: 571388 (2020). PubMed PMID 33330450.Split-GFP probe plasmid usable in cultured cells
Catalog # Name of resource 1 Name of reource 2 Localization RDB16041 pMM80 pcDNA3.1_Tom20N-3xFLAG-GFP1-10 mitochondria RDB16042 pMM82 pcDNA3.1-Tom70N-3xFLAG-GFP1-10 mitochondria RDB18662 pMM83 pcDNA3.1-Tom70N-3xFLAG-GFP11 mitochondria RDB18665 pMM189 pIRESNeo3-TOMM70 1-70-3xFLAG-GFP11 mitochondria RDB18663 pMM86 pcDNA3.1-ERj1N-V5-GFP1-10 endoplasmic reticulum RDB16047 pMM87 pcDNA3.1_ERj1N-V5-GFP11 endoplasmic reticulum RDB16048 pMM89 pcDNA3.1_Sec63N-V5-GFP11 endoplasmic reticulum RDB18655 pMM104 pcDNA3.1_Lamp1-HA_GFP11 lysosome Split-GFP probe plasmid usable in budding yeast (for genome integration)
Catalog # Name of resource 1 Name of reource 2 Localization DNA size (bp) RDB18656 pYC135 pBS-GPDp-Ifa38-V5-GFP11-CyC1ter-natNT2 endoplasmic reticulum ca. 3,700 RDB18657 pYC136 pBS-GPDp-Erg6-V5-GFP11-CyC1ter-natNT2 lipid droplets ca. 3,800 RDB18658 pYC137 pBS-GPDp-DPP1-V5-GFP11-CyC1ter-natNT2 vacuole ca. 3,600 RDB18659 pYC141 pBS-GPDp-Ifa38-GFP1-10-CyC1ter-hphMX endoplasmic reticulum ca. 4,500 RDB18660 pYC143 pBS-GPDp-Tom71-GFP1-10-CyC1ter-hphMX mitochondria ca. 5,300 RDB18661 pYC144 pBS-GPDp-Pex3N-GFP1-10-CyC1ter-hphMX peroxisome ca. 3,600 Split-GFP probe plasmid usable in budding yeast (for plasmid expressionn)
Catalog # Name of resource 1 Name of reource 2 Localization Marker gene RDB16037 pSFL22 pRS316-GPDp-Tom71-GFP1-10 mitochondria URA3 RDB16038 pSFL23 pRS314-GPDp-Tom71-GFP11 mitochondria TRP1 RDB16039 pSFL75 pRS314-GPDp-Tom71-V5-GFP11 mitochondria TRP1 RDB16040 pSFL15 pRS314-GPDp-YNR021W-GFP11 endoplasmic reticulum TRP1 RDB16043 pSFL16 pRS316-GPDp-Ifa38-GFP1-10 endoplasmic reticulum URA3 RDB16044 pSFL18 pRS314-GPDp-Ifa38-GFP11 endoplasmic reticulum TRP1 RDB16045 pSFL86 pRS313-GPDp-Ifa38-3xFLAG-GFP11 endoplasmic reticulum HIS3 RDB16046 pSFL59 pRS314-GPDp-Ifa38-mCherry-GFP11 endoplasmic reticulum TRP1 RDB16049 pSFL19 pRS316-GPDp-Vph1-GFP1-10 vacuole URA3 RDB16050 pSFL108 pRS314-GPDp-Dpp1-V5-GFP11 vacuole TRP1 RDB16051 pSFL26 pRS316-GPDp-Pex3N-GFP1-10 peroxisome URA3 RDB16052 pSFL96 pRS316-GPDp-Pex3N-3xFLAG-GFP11 peroxisome URA3 RDB16053 pSFL28 pRS316-GPDp-Erg6-GFP1-10 lipid droplets URA3 RDB16054 pSFL67 pRS314-GPDp-Erg6-3xFLAG-GFP11 lipid droplets TRP1 RDB18571 pSFL17 pRS315-GPDp-Ifa38-GFP1-10 endoplasmic reticulum LEU2 RDB18572 pSFL61 pRS316-GPDp-Ifa38-mCherry-GFP11 endoplasmic reticulum URA3 RDB18573 pSFL66 pRS314-GPDp-Ifa38-3xFLAG-GFP11 endoplasmic reticulum TRP1 RDB18574 pSFL68 pRS314-GPDp-Erg6-V5-GFP11 lipid droplets TRP1 RDB18577 pSFL76 pRS314-GPDp-Ifa38-V5-GFP11 endoplasmic reticulum TRP1 Backborn constructs
Catalog # Name of resource Descriptiion RDB18567 pSFL9 pRS316-GPDp-GFP1-10 Expression vector of the first ten beta-strands (GFP1-10). RDB18568 pSFL10 pRS314-GPDp-GFP1-10 Expression vector of the first ten beta-strands (GFP1-10). RDB18570 pSFL12 pRS314-GPDp-GFP11 Expression vector of the the last 11th beta-strand (GFP11). RDB18575 pSFL73 pRS314-GPDp-3xFLAG-GFP11 Expression vector of 3xFLAG-GFP11. RDB18576 pSFL74 pRS314-GPDp-V5-GFP11 Expression vector of V5-GFP11.
GPI anchored Reconstitution-Activated Proteins Highlight Intercellular Connections (GRAPHIC)
- Genetically Encoded Fluorescent Indicator GRAPHIC Delineates Intercellular Connections.
Kinoshita, N., Huang, A.J.Y., McHugh, T.J., Suzuki, S.C., Masai, I., Kim, I.H., Soderling, S.H., Miyawaki, A., Shimogori, T.
iScience 15: 28-38 (2019). PubMed PMID 31026667. - Diffusible GRAPHIC to visualize morphology of cells after specific cell-cell contact.
Kinoshita, N., Huang, A.J.Y., McHugh, T.J., Miyawaki, A., Shimogori, T.
Sci. Rep. 10 (1): 14437 (2020). PubMed PMID 32879377.Catalog no. Name of clone Descriptiion RDB15869 pCSII-CMV-MCS _n-GRAPHIC Lentiviral expression vector of GRAPHIC probe (LZA). RDB15870 pCSII-CMV-MCS_n-GRAPHIC T2A H2B-mCherry Lentiviral expression vector of GRAPHIC probe (LZA). RDB15871 pCSII-CMV-MCS_B (BFP type) n-GRAPHIC T2A H2B-mCherry Lentiviral expression vector of GRAPHIC probe (LZA). RDB15872 pCSII-CMV-MCS _c-GRAPHIC Lentiviral expression vector of GRAPHIC probe (LZB). RDB15873 pCSII-CMV-MCS _c-GRAPHIC T2A H2B-Azurite Lentiviral expression vector of GRAPHIC probe (LZB). RDB15874 pCSII-CMV-MCS _Y (YFP- type) c-GRAPHIC T2A H2B-Azurite Lentiviral expression vector of GRAPHIC probe (LZB). RDB19166 AAV:NT-probe-nuc-mCherry Adeno-associated viral vector encoding the dGRAPHIC NT probe. RDB19167 AAV:CT-probe-cyto-mCherry Adeno-associated viral vector encoding the dGRAPHIC CT probe.
FPs with tissue specific promoter
Synapsin I (SYN) promoter
| Catalog no. | Name of clone | Running title |
|---|---|---|
| RDB15107 | pBSIISK-SYN-GFP-BGHpA | Expression vector of GFP driven by the neuron-specific promoter (human synapsin I (SYN)). |
| RDB15109 | pENTR1A-SYN-GFP-BGHpA(r) | Gateway(R) entry vector of GFP expression unit driven by the neuron-specific promoter (human synapsin I (SYN)). |
| RDB15113 | pAAV2-SYN-GFP-BGHpA | AAV2 expression vector of GFP driven by the neuron-specific promoter (human synapsin I (SYN)). |
Broad expression
| Catalog no. | Name of clone | Running title |
|---|---|---|
| RDB06071 | pCMFlag_EGFP | Expression vector of GFP, with FLAG-tag, control vector |
| RDB15108 | pENTR1A-CMV-GFP-BGHpA(r) | Gateway(R) entry vector of GFP expression unit driven by the ubiquious promoter (CMV) |
| RDB15112 | pAAV2-CMV-GFP-BGHpA | AAV2 expression vector of GFP driven by the ubiquious promoter (CMV) |
(GRP0053e 2018.04.24 T.M.)
2022.05.27
