Visualization of Organelles
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Organelle marker
Visualizing multiple inter-organelle contact sites
Organelle marker
We are providing genetic resources for visualization of organelles such as mitochondria and nucleus. Each clone contains an organelle localization signal sequence fused with fluorescent proteins or epitope tags. Organelles can be detected by fluorescence or by detecting epitope tags with antibodies.
Of those, 12 species of organelles can be observed by Nano-lantern luminescent and fluorescent proteins developed by Drs. Okada and Takai of the RIKEN Center for Biosystems Dynamics Research (BDR) and Nagai and their colleague of the Osaka University [Takai, A. et al., Proc Natl Acad Sci U S A. 112 (14): 4352-4356, 2015]. Nano-lantern is a brighter luminescent protein, consisting of luciferase and fluorescent protein. Three colors (yellow, cyan and orange) of Nano-lanterns are available for each localization signal.
Actin (β-actin)
- Fluorescent protein porbe
Catalog no. Name of clone Running title RDB18314 pActin-mAG1 CoralHue™ β-Actin-targeted monomeric Azami-Green 1 RDB18319 pActin-hmKO1 CoralHue™ β-Actin-targeted monomeric Kusabira-Orange 1
Actin (F-actin)
- Nano-lantern clones
Catalog no. Name of clone Running title RDB13348 pCNL-N1_ITPKA Cyan Nano-lantern for labeling of F-actin by the rat inositol triphosphate 3-kinase A. RDB13347 pYNL-N1_ITPKA Yellow Nano-lantern for labeling of F-actin by the rat inositol triphosphate 3-kinase A. RDB13349 pONL-N1_ITPKA Orange Nano-lantern for labeling of F-actin by the rat inositol triphosphate 3-kinase A.
Cytoskeleton and focal adhesion
- Nano-lantern clones
Catalog no. Name of clone Running title RDB13351 pCNL-N1_vinculin Cyan Nano-lantern with the chicken vinculin. RDB13350 pYNL-N1_vinculin Yellow Nano-lantern with the chicken vinculin. RDB13352 pONL-N1_vinculin Orange Nano-lantern with the chicken vinculin.
Dense-core vesicles
- Fluorescent protein porbe
Catalog no. Name of clone Running title RDB15118 pNPY-Venus-N1 Venus fluorescent protein targeting localization in neurites. Mammalian expression. RDB15119 NPY-Venus/pEF-BOS Venus fluorescent protein targeting localization in neurites. Mammalian expression.
Endoplasmic reticulum
- Fluorescent protein porbe
Catalog no. Name of clone Running title RDB18312 pER-mAG1 CoralHue™ ER-targeted monomeric Azami-Green 1 RDB18326 pER-mAG407 CoralHue™ ER-targeted monomeric Azami-Green407 RDB18317 pER-mKO1 CoralHue™ ER-targeted monomeric Kusabira-Orange 1
Focal adhesions
- Nano-lantern clones
Catalog no. Name of clone Running title RDB13342 pCNL-N1_zyxin Cyan Nano-lantern for labeling of focal adhesions by the mouse zyxin. RDB13341 pYNL-N1_zyxin Yellow Nano-lantern for labeling of focal adhesions by the mouse zyxin. RDB13343 pONL-N1_zyxin Orange Nano-lantern for labeling of focal adhesions by the mouse zyxin.
Golgi apparatus
- Epitope-tagged protein probe
Catalog no. Name of clone Running title RDB03423 FLAG-Rab27A Expression vector of mouse Rab27A cDNA which can be used as secretory granule and melanosomme markers. - Fluorescent protein porbe
Catalog no. Name of clone Running title RDB13353 pEGFP-C1-mouse Rab33B Expression vector of mouse RAB33B, N-terminal EGFP-tag. - Nano-lantern clones
Catalog no. Name of clone Running title RDB13354 pCNL-N1_Golgi Cyan Nano-lantern for labeling of Golgi apparatus by the N-terminal 81 amino acids of human beta 1,4-galactosyltransferase. RDB13353 pYNL-N1_Golgi Yellow Nano-lantern for labeling of Golgi apparatus by the N-terminal 81 amino acids of human beta 1,4-galactosyltransferase. RDB13355 pONL-N1_Golgi Orange Nano-lantern for labeling of Golgi apparatus by the N-terminal 81 amino acids of human beta 1,4-galactosyltransferase.
Kinetochore
- Fluorescent protein porbe
Catalog no. Name of clone Running title RDB13516 pGEMHE-mHec1-sfGFP Labeling outer kinetochore. Plasmid for in vitro transcription of mouse Hec1 (Ndc80) in mouse oocyte. RDB13518 pGEMHE-tdTomato-mCENP-C Labeling inner kinetochore. Plasmid for in vitro transcription of mouse CENP-C in mouse oocyte.
Lysosome
- Nano-lantern clones
Catalog no. Name of clone Running title RDB13345 pCNL-N1_LAMP1 Cyan Nano-lantern for labeling of lysosome by the rat LAMP1. RDB13344 pYNL-N1_LAMP1 Yellow Nano-lantern for labeling of lysosome by the rat LAMP1. RDB13346 pONL-N1_LAMP1 Orange Nano-lantern for labeling of lysosome by the rat LAMP1. - Related article
Melanosome
- Epitope-tagged protein probe
Catalog no. Name of clone Running title RDB14634 pEF-T7-GST-Kif1c-tail-879-1100/M-INK M-INK, a novel tool for tagging the mature melanosomes. Expression vector of T7- and GST- tagged C terminal half tail region of the mouse Kif1c. RDB03423 FLAG-Rab27A Expression vector of mouse Rab27A cDNA which can be used as secretory granule and melanosomme markers. - Fluorescent protein porbe
Catalog no. Name of clone Running title RDB15184 pCMV-Mreg delta C/MST-mStr MST tag, a novel melanosome-targeting tag. - Related article
Mitochondria
- Epitope-tagged protein probe
Catalog no. Name of clone Running title RDB17747 pN1-OPA1(123Nt)-Halo Halo Tag, Mitochondrial intermembrane space localization RDB17750 pN1-TOMM20-Halo Halo Tag, Mitochondrial outer membrane localization - Fluorescent protein probe
Catalog no. Name of clone Running title RDB18323 pMT-mMiCy1 CoralHue™ Mitochondria-targeted monomeric Midoriishi-Cyan 1 RDB18311 pMT-mAG1 CoralHue™ Mitochondria-targeted monomeric Azami-Green RDB18316 pMT-mKO1 CoralHue™ Mitochondria-targeted monomeric Kusabira-Orange 1 RDB18321 pMT-mKeima-Red CoralHue™ Mitochondria-targeted mKeima-Red RDB15238 mt-mKeima (h)/pIND(SP1) Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal. RDB15456 mt-mKeima/pcDNA3 Expression vector of monomeric Keima-Red (mKeima) with mitochondria localization signal. - Nano-lantern clones
Catalog no. Name of clone Running title RDB13330 pCNL-N1_Mito Cyan Nano-lantern for labeling of mitochondria by the mitochondrial targeting sequence from subunit VIII of human cytochrome c oxdase. RDB13329 pYNL-N1_Mito Yellow Nano-lantern for labeling of mitochondria by the mitochondrial targeting sequence from subunit VIII of human cytochrome c oxdase. RDB13331 pONL-N1_Mito Orange Nano-lantern for labeling of mitochondria by the mitochondrial targeting sequence from subunit VIII of human cytochrome c oxdase.
Nuclei
- Nano-lantern clones
Catalog no. Name of clone Running title RDB13333 pCNL-N1_3xNLS Cyan Nano-lantern for labeling of nuclei by the nuclear localization signal of SV40. RDB13332 pYNL-N1_3xNLS Yellow Nano-lantern for labeling of nuclei by the nuclear localization signal of SV40. RDB13334 pONL-N1_3xNLS Orange Nano-lantern for labeling of nuclei by the nuclear localization signal of SV40.
Nuclei in interphase
- Nano-lantern clones
Catalog no. Name of clone Running title RDB13363 pCNL-C1_H2B Cyan Nano-lantern for labeling of nuclei in interphase by the human histone H2B. RDB13362 pYNL-C1_H2B Yellow Nano-lantern for labeling of nuclei in interphase by the human histone H2B. RDB13364 pONL-C1_H2B Orange Nano-lantern for labeling of nuclei in interphase by the human histone H2B.
Nucleoli
- Nano-lantern clones
Catalog no. Name of clone Running title RDB13369 pCNL-C1_fibrillarin Cyan Nano-lantern for labeling of nucleoli by the human fibrillarin. RDB13368 pYNL-C1_fibrillarin Yellow Nano-lantern for labeling of nucleoli by the human fibrillarin. RDB13370 pONL-C1_fibrillarin Orange Nano-lantern for labeling of nucleoli by the human fibrillarin.
Nucleoplasm
- Fluorescent protein probe
Catalog no. Name of clone Running title RDB18325 pNP-hKCy1 CoralHue™ Nucleoplasm-targeted humanized Kusabira-Cyan 1 RDB18315 pNP-AG CoralHue™ Nucleoplasm-targeted Azami-Green RDB18320 pNP-KO1 CoralHue™ Nucleoplasm-targeted Kusabira-Orange 1 RDB18327 pNP-hdKeima570 CoralHue™ Nucleoplasm-targeted humanized dimeric Keima570 RDB18324 pNP-hdKeima-Red CoralHue™ Nucleoplasm-targeted humanized dimeric Keima-Red
Peroxisome
- Nano-lantern clones
Catalog no. Name of clone Running title RDB13360 pCNL-C1_PTS1 Cyan Nano-lantern for labeling of peroxisome by the peroxisomal targeting signal 1 (tripeptide SKL). RDB13336 pCNL-N1_PTS2 Cyan Nano-lantern for labeling of peroxisome by the peroxisomal targeting signal 2. RDB13359 pYNL-C1_PTS1 Yellow Nano-lantern for labeling of peroxisome by the peroxisomal targeting signal 1 (tripeptide SKL). RDB13335 pYNL-N1_PTS2 Yellow Nano-lantern for labeling of peroxisome by the peroxisomal targeting signal 2. RDB13361 pONL-C1_PTS1 Orange Nano-lantern for labeling of peroxisome by the peroxisomal targeting signal 1 (tripeptide SKL). RDB13337 pONL-N1_PTS2 Orange Nano-lantern for labeling of peroxisome by the peroxisomal targeting signal 2.
Plasma membrane and endosome
- Epitope-tagged protein probe
Catalog no. Name of clone Running title RDB14677 pEF-BOS FLAG-mouse Rab5A Expression vector of mouse Rab5A, which is localized in early endosome. RDB14678 pEF-BOS FLAG-mouse Rab7 Expression vector of mouse Rab7, which is localized in late endosome. - Fluorescent protein probe
Catalog no. Name of clone Running title RDB18313 pPM-mAG1 CoralHue™ Plasma Membrane-targeted monomeric Azami-Green 1. RDB18318 pPM-mKO1 CoralHue™ Plasma Membrane-targeted mKusabira-Orange 1. RDB18322 pPM-mKeima-Red CoralHue™ Plasma Membrane-targeted mKeima-Red. RDB15030 pmStr-C1-mouse Rab11A Expression vector of mouse Rab11A, Recycling endosome marker. RDB13501 pCAG/tdiRFP-CAAX Marker of plasma membrane. Mammalian expression plasmid of CAAX box protein. - Nano-lantern clones
Catalog no. Name of clone Running title RDB13357 pCNL-C1_RhoB Cyan Nano-lantern for labeling of plasma membrane and endosomes by the human RhoB. RDB13366 pCNL-C1_Rab11a Cyan Nano-lantern for labeling of recycling endosomes by the mouse Rab11a. RDB13356 pYNL-C1_RhoB Yellow Nano-lantern for labeling of plasma membrane and endosomes by the human RhoB. RDB13365 pYNL-C1_Rab11a Yellow Nano-lantern for labeling of recycling endosomes by the mouse Rab11a. RDB13358 pONL-C1_RhoB Orange Nano-lantern for labeling of plasma membrane and endosomes by the human RhoB. RDB13367 pONL-C1_Rab11a Orange Nano-lantern for labeling of recycling endosomes by the mouse Rab11a. - Related article
Sphingolipid marker
- Lipid rafts are small lipid domains on the cell membrane and are thought to play an important role in signal transduction, endocytosis and more. We provide fluorescent probes for sphingomyelin and cholesterol lipid domains.
- Nakanori: sphingomyelin and cholesterol lipid domain (lipid raft)
Catalog no. Name of clone Running title RDB14301 pET28/His6-EGFP-Nakanori Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of His6-EGFP-tagged G. frondosa Nakaniori. - D4 toxin: cholesterol rich domain
Catalog no. Name of clone Running title RDB13961 pET28/His6-EGFP-D4 Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of His6-EGFP-tagged perfringolysin theta toxin D4 of C. perfringens. RDB14300 pET28/His6-mCherry-D4 Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of His6-mCherry-tagged perfringolysin theta toxin D4 of C. perfringens. RDB15469 pET28/Dronpa-theta-D4 Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of perfringolysin theta toxin D4 of C. perfringens. - lysenin: sphingomyelin
Catalog no. Name of clone Running title RDB13498 pET28/His6-EGFP-NT-Lys Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin. RDB13499 pET28/His6-mCherry-NT-Lys Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin. RDB13958 pMAL/MBP-lysenin Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of MBP-tagged lysenin. RDB13959 pQE30/His6-Venus-NT-Lys Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of His6-Venus-tagged non toxic lysenin. RDB13960 pQE30/His6-mRFP-NT-Lys Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of His6-mRFP-tagged non toxic lysenin. RDB15470 pET28/Dronpa-NT-Lys Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin. - Other markers
Catalog no. Name of clone Running title RDB13496 pET28/EqtII-EGFP-His6 Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of Eqinatoxin II. RDB13497 pET28/EqtII(8-69)-EGFP-His6 Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Eqinatoxin II. RDB13962 pET28/PlyA2-EGFP-His6 Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged PlyA2. RDB13963 pET28/Oly-EGFP-His6 Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged Oly. RDB13964 pET28/EryA-EGFP-His6 Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged EryA. - Related article
Tubulin (β-tubulin)
- Nano-lantern clones
Catalog no. Name of clone Running title RDB13339 pCNL-N1_TUBB5 Cyan Nano-lantern for labeling of beta-tubulin by the mouse beta-tubulin class V. RDB13338 pYNL-N1_TUBB5 Yellow Nano-lantern for labeling of beta-tubulin by the mouse beta-tubulin class V. RDB13340 pONL-N1_TUBB5 Orange Nano-lantern for labeling of beta-tubulin by the mouse beta-tubulin class V.
Visualizing multiple inter-organelle contact sites
Fluorescent protein probe that can visualize the multiple inter-organelle contact sites in cells.
- Divided GFP-fragment have a feature that they emit fluorescence again by reconstitution when each fragment is in close proximity. When labelled organelles such as mitochondria and ERs by the divided GFPs exist together, the contact sites in cells are visualized by the reconstituted fluorescent protein. The vectors of fluorescent protein probes developed by Dr. Yasushi Tamura of the Yamagata University, Dr. Toshiya Endo of the Kyoto Sangyo University and their colleague are available from the DNA Bank.
- Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system.
Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y.
Sci. Rep. 8 (1): 6175 (2018). PubMed PMID 29670150.Catalog no. Name of resource Descriptiion RDB16037 Tom71-GFP1-10 Expression vector of S. cerevisiae protein channel TOM71 (Tom 71) for visualizing organelle contact sites in vivo. RDB16038 Tom71-GFP11 Expression vector of S. cerevisiae protein channel TOM71 (Tom 71) for visualizing organelle contact sites in vivo. RDB16039 Tom71-V5-GFP11 Expression vector of S. cerevisiae protein channel TOM71 (Tom 71) for visualizing organelle contact sites in vivo. RDB16040 YNR021W-GFP11 Expression vector of S. cerevisiae endoplasmic reticulum (ER) protein YNR021W (hypothetical protein) for visualizing organelle contact sites in vivo. RDB16041 Tom20N-FLAG-GFP1-10 Expression vector of human translocase of outer mitochondrial membrane 20 (Tom20), N-terminal segments 1-33 for visualizing organelle contact sites in vivo. RDB16042 Tom70N-FLAG-GFP1-10 Expression vector of human translocase of outer mitochondrial membrane 70 (Tom70), N-terminal segments 1-70v RDB16043 Ifa38-GFP1-10 Expression vector of S. cerevisiae ketoreductase (Ifa38) for visualizing organelle contact sites in vivo. RDB16044 Ifa38-GFP11 Expression vector of S. cerevisiae ketoreductase (Ifa38) for visualizing organelle contact sites in vivo. RDB16045 Ifa38-FLAG-GFP11 Expression vector of S. cerevisiae ketoreductase (Ifa38) for visualizing organelle contact sites in vivo. RDB16046 Ifa38-mCherry-GFP11 Expression vector of S. cerevisiae ketoreductase (Ifa38) for visualizing organelle contact sites in vivo. RDB16047 ERj1N-V5-GFP11 Expression vector of human DnaJ Heat Shock Protein Family (Hsp40) Member C1, N-terminal segments 1-200 for visualizing organelle contact sites in vivo. RDB16048 Sec63N-V5-GFP11 Expression vector of human SEC63 homolog, protein translocation regulator (Sec63), N-terminal segments 1-240 for visualizing organelle contact sites in vivo. RDB16049 Vph1-GFP1-10 Expression vector of S. cerevisiae H(+)-transporting V0 sector ATPase subunit a (Vph1) for visualizing organelle contact sites in vivo. RDB16050 Dpp1-V5-GFP11 Expression vector of S. cerevisiae bifunctional diacylglycerol diphosphate phosphatase/phosphatidate phosphatase (Dpp1) for visualizing organelle contact sites in vivo. RDB16051 Pex3N-GFP1-10 Expression vector of S. cerevisiae Pex3p (Pex3), N-terminal segments 1-60 for visualizing organelle contact sites in vivo. RDB16052 Pex3N-FLAG-GFP11 Expression vector of S. cerevisiae Pex3p (Pex3), N-terminal segments 1-60 for visualizing organelle contact sites in vivo. RDB16053 Erg6-GFP1-10 Expression vector of S. cerevisiae sterol 24-C-methyltransferase (Erg6) for visualizing organelle contact sites in vivo. RDB16054 Erg6-FLAG-GFP11 Expression vector of S. cerevisiae sterol 24-C-methyltransferase (Erg6) for visualizing organelle contact sites in vivo.
(GRP0053e 2018.04.24 T.M.)
2020.09.28
