Series of plasmids that confere biosynthesis of carotenoids in E. coli.
List of plasmids in Misawa, N. et al. Methods Enzymol. 670: 87-137 (2022). PMID 35871847.
pACYC184 (Cmr)-based plasmid (without IDI)
| Catalog # | Name of resource | Carotenoid biosynthesis genes | Running title | Reference |
|---|---|---|---|---|
| RDB19483 | pACCRT-EB | crtE, crtB | pACYC184 vector-based plasmid for biosynthesis of phytoene in E. coli (crtE, crtB). | Misawa, N. et al. J. Bacteriol. 177 (22): 6575-6584 (1995). PMID 7592436. [link to RRC of NBRP] |
| RDB19484 | pACCRT-EIB | crtE, crtB, crtI | pACYC184 vector-based plasmid for biosynthesis of lycopene in E. coli (crtE, crtB, crtI). | Misawa, N. et al. J. Bacteriol. 177 (22): 6575-6584 (1995). PMID 7592436. [link to RRC of NBRP] |
| RDB19485 | pACCRT-EB-RcI | crtE, crtB, Rc-crtI | pACYC184 vector-based plasmid for biosynthesis of neurosporene in E. coli (crtE, crtB, Rc-crtI). | Takaichi, S. et al. 241 (1): 291-296 (1996). PMID 8898919. [link to RRC of NBRP] |
| RDB19486 | pACCAR16 delta crtX | crtE, crtB, crtI, crtY | pACYC184 vector-based plasmid for biosynthesis of β-carotene in E. coli (crtE, crtB, crtI, crtY). | Misawa, N. et al. J. Bacteriol. 177 (22): 6575-6584 (1995). PMID 7592436. [link to RRC of NBRP] |
| RDB19487 | pACCAR25 delta crtX | crtE, crtB, crtI, crtY, crtZ | pACYC184 vector-based plasmid for biosynthesis of zeaxanthin in E. coli (crtE, crtB, crtI, crtY, crtZ). | Misawa, N. et al. J. Bacteriol. 177 (22): 6575-6584 (1995). PMID 7592436. [link to RRC of NBRP] |
| RDB19488 | pAC-Cantha | crtE, crtB, crtI, crtY, crtW | pACYC184 vector-based plasmid for biosynthesis of canthaxanthin in E. coli (crtE, crtB, crtI, crtY, crtW). | Nishida, Y. et al. Appl. Environ. Microbiol. 71 (8): 4286-4296 (2005). PMID 16085816. [link to RRC of NBRP] |
| RDB19489 | pAC-Asta | crtE, crtB, crtI, crtY, crtZ, crtW | pACYC184 vector-based plasmid for biosynthesis of astaxanthin in E. coli (crtE, crtB, crtI, crtY, crtZ, crtW). | Nishida, Y. et al. Appl. Environ. Microbiol. 71 (8): 4286-4296 (2005). PMID 16085816. [link to RRC of NBRP] |
pRK404 (Tcr)-based plasmid
| Catalog # | Name of resource | Carotenoid biosynthesis genes | Running title | Reference |
|---|---|---|---|---|
| RDB19490 | pRK-idi | XdIDI | Expression plasmid of the isopentenyl diphoshate (IPP) isomerase gene (IDI), which is used to incerease carotenoid production with another carotenogenic plasmid in E. coli (XdIDI). | Albrecht, M. et al. Biotechnology Letters 21: 791?795 (1999). [link to RRC of NBRP] |
pACYC184 (Cmr)-based plasmid (with IDI)
| Catalog # | Name of resource | Carotenoid biosynthesis genes | Running title | Reference |
|---|---|---|---|---|
| RDB19491 | pACHP-Phy | HpIDI, crtE, crtB | pACYC184 vector-based plasmid for biosynthesis of phytoene (addtionally including the HpIDI gene) in E. coli. plasmid pACCRT-EB with HpIDI (HpIDI, crtE, crtB). | Takemura, M. et al. BMC Zoology 6:19 (2021). [link to RRC of NBRP] |
| RDB19492 | pACHP-Lyc | HpIDI, crtE, crtB, crtI | pACYC184 vector-based plasmid for biosynthesis of lycopene (addtionally including the HpIDI gene) in E. coli. Plasmid pACCRT-EIB with HpIDI (HpIDI, crtE, crtB, crtI). | Takemura, M. et al. BMC Zoology 6:19 (2021). [link to RRC of NBRP] |
| RDB19493 | pACHP-Beta | HpIDI, crtE, crtB, crtI, crtY | pACYC184 vector-based plasmid for biosynthesis of β-carotene (addtionally including the HpIDI gene) in E. coli. plasmid pACCAR16ΔcrtX with HpIDI (HpIDI, crtE, crtB, crtI, crtY). | Takemura, M. et al. Planta 241 (3): 699-710 (2015). PMID 25467956. [link to RRC of NBRP] |
| RDB19494 | pACHP-Zea | HpIDI, crtE, crtB, crtI, crtY, crtZ | pACYC184 vector-based plasmid for biosynthesis of zeaxanthin (addtionally including the HpIDI gene) in E. coli. plasmid pACCAR25ΔcrtX with HpIDI (HpIDI, crtE, crtB, crtI, crtY, crtZ). | Takemura, M.et al. Tetrahedron Letters 56(44): 6063-6065 (2015). [link to RRC of NBRP] |
pACYC184 (Tcr)-based plasmid (with IDI)
| Catalog # | Name of resource | Carotenoid biosynthesis genes | Running title | Reference |
|---|---|---|---|---|
| RDB19495 | pAC-HIEB | HpIDI, crtE, crtB | pACYC184 vector-based plasmid for biosynthesis of phytoene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB). | Misawa, N. et al. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP] |
| RDB19496 | pAC-HIEBI | HpIDI, crtE, crtB, crtI | pACYC184 vector-based plasmid for biosynthesis of lycopene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI). | Takemura, M. et al. BMC Zoology 6:19 (2021). [link to RRC of NBRP] |
| RDB19497 | pAC-HIEBIA | HpIDI, crtE, crtB, crtI, cruA | pACYC184 vector-based plasmid for biosynthesis of γ-carotene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, cruA). | Takemura, M. et al. Synth. Biol. (Oxf) 6 (1): ysab012 (2021). PMID 34712837. [link to RRC of NBRP] |
| RDB19498 | pAC-HIEBIY | HpIDI, crtE, crtB, crtI, crtY | pACYC184 vector-based plasmid for biosynthesis of β-carotene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY). | Misawa, N. et al. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP] |
| RDB19499 | pAC-HIEBIYZ | HpIDI, crtE, crtB, crtI, crtY, crtZ | pACYC184 vector-based plasmid for biosynthesis of zeaxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY, crtZ). | Misawa, N. et al. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP] |
| RDB19500 | pAC-HIEBIYZW | HpIDI, crtE, crtB, crtI, crtY, crtZ, crtW | pACYC184 vector-based plasmid for biosynthesis of astaxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY, crtZ, crtW). | Misawa, N. et al. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP] |
| RDB19501 | pAC-HIEBIYZG | HpIDI, crtE, crtB, crtI, crtY, crtZ, crtG | pACYC184 vector-based plasmid for biosynthesis of nostoxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY, crtZ, crtG). | Misawa, N. et al. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP] |
| RDB19502 | pAC-HIEBI-ApCrtYB3 | HpIDI, crtE, crtB, crtI, ApCrtYB3 | pACYC184 vector-based plasmid for biosynthesis of β,γ-carotene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, ApCrtYB3). | Takemura, M. et al. BMC Zoology 6:19 (2021). [link to RRC of NBRP] |
| RDB19503 | pAC-HIEBI-ApCrtYB1 | HpIDI, crtE, crtB, crtI, ApCrtYB1 | pACYC184 vector-based plasmid for biosynthesis of torulene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, ApCrtYB1). | Takemura, M. et al. BMC Zoology 6:19 (2021). [link to RRC of NBRP] |
| RDB19504 | pAC-HIM | HpIDI, crtM | pACYC184 vector-based plasmid for biosynthesis of 15-cis-4,4′-diapophytoene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtM). | Takemura, M. et al. Microb. Cell Fact. 20 (1): 194 (2021). PMID 34627253. [link to RRC of NBRP] |
| RDB19505 | pAC-HIMN | HpIDI, crtM, crtN | pACYC184 vector-based plasmid for biosynthesis of 4,4′-diaponeurosporene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtM, crtN). | Takemura, M. et al. Microb. Cell Fact. 20 (1): 194 (2021). PMID 34627253. [link to RRC of NBRP] |
pRK404 (Tcr)-based plasmid (with IDI)
| Catalog # | Name of resource | Carotenoid biosynthesis genes | Running title | Reference |
|---|---|---|---|---|
| RDB19506 | pRK-HIEB | HpIDI, crtE, crtB | pRK404 vector-based plasmid for biosynthesis of phytoene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB). | Albrecht, M. et al. Biotechnology Letters 21: 791?795 (1999). [link to RRC of NBRP] |
| RDB19507 | pRK-HIEBI | HpIDI, crtE, crtB, crtI | pRK404 vector-based plasmid for biosynthesis of lycopene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI). | Misawa, N. et al. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP] |
| RDB19508 | pRK-HIEBIY | HpIDI, crtE, crtB, crtI, crtY | pRK404 vector-based plasmid for biosynthesis of β-carotene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY). | Misawa, N. et al. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP] |
| RDB19509 | pRK-HIEBIYZ | HpIDI, crtE, crtB, crtI, crtY, crtZ | pRK404 vector-based plasmid for biosynthesis of zeaxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY, crtZ). | Misawa, N. et al. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP] |
| RDB19510 | pRK-HIEBIYZW | HpIDI, crtE, crtB, crtI, crtY, crtZ, crtW | pRK404 vector-based plasmid for biosynthesis of astaxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY, crtZ, crtW). | Misawa, N. et al. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP] |
| RDB19511 | pRK-HIEBIYZG | HpIDI, crtE, crtB, crtI, crtY, crtZ, crtG | pRK404 vector-based plasmid for biosynthesis of nostoxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY, crtZ, crtG). | Misawa, N. et al. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP] |
| RDB19512 | pRK-HIEBI-MpLCYb delta TP-MpLCYe-Z-Epg | HpIDI, crtE, crtB, crtI, MpLCYb, MpLCYe, crtZ, Pag-crtE | pRK404 vector-based plasmid for biosynthesis of zeinoxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, MpLCYb, MpLCYe, crtZ, Pag-crtE). | Takemura, M. et al. Synth. Biol. (Oxf) 6 (1): ysab012 (2021). PMID 34712837. [link to RRC of NBRP] |
| RDB19513 | pRK-HIEBIYZ-FhCCD2 | HpIDI, crtE, crtB, crtI, crtY, crtZ, FhCCD2 | pRK404 vector-based plasmid for biosynthesis of crocetindial (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY, crtZ, FhCCD2). | Misawa, N. et al. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP] |
| RDB19514 | pRK-HIEBIYZ-FhCCD2-NcALD8 | HpIDI, crtE, crtB, crtI, crtY, crtZ, FhCCD2, NcALD8 | pRK404 vector-based plasmid for biosynthesis of crocetin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY, crtZ, FhCCD2, NcALD8). | Misawa, N. et al. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP] |
Complementary plasmids for a series of carotenoid biosynthesis
| Catalog # | Name of resource | Carotenoid biosynthesis genes | Running title | Reference |
|---|---|---|---|---|
| RDB19515 | pUCBre-W | Bre-crtW | crtW-expression plasmid for production of astaxanthin and other ketocarotenoids when coexpressed with a plasmid to biosynthesize zeaxanthin in E. coli. | Nishida, Y. et al. Appl. Environ. Microbiol. 71 (8): 4286-4296 (2005). PMID 16085816. [link to RRC of NBRP] |
| RDB19516 | pUCBre-O11 | crtG (Bre-crtG) | crtG-expression plasmid for production of nostoxanthin and caloxanthin when coexpressed with a plasmid to biosynthesize zeaxanthin in E. coli. | Nishida, Y. et al. Appl. Environ. Microbiol. 71 (8): 4286-4296 (2005). PMID 16085816. [link to RRC of NBRP] |
| RDB19517 | pUC-ApTor | ApTor | ApTOR-expression plasmid for production of 3,4-didehydrolycopene and lycopene when coexpressed with a plasmid to biosynthesize phytoene in E. coli. | Takemura, M. et al. BMC Zoology 6:19 (2021). [link to RRC of NBRP] |
| RDB19518 | pUC-CaZEP | CaZEP | CaZEP-expression plasmid for production of violaxanthin and antheraxanthin when coexpressed with a plasmid to biosynthesize zeaxanthin in E. coli. | Takemura, M. et al. Appl. Microbiol. Biotechnol. 103 (23-24): 9393-9399 (2019). PMID 31673744. [link to RRC of NBRP] |
| RDB19519 | pETDuet-MpLCYb/MpLCYe | MpLCYb, MpLCYe | MpLCYb- and MpLCYe-expression plasmid for production of α-carotene (and β-carotene) when coexpressed with a plasmid to biosynthesize lycopene in E. coli. | Takemura, M. et al. Planta 241 (3): 699-710 (2015). PMID 25467956. [link to RRC of NBRP] |
| RDB19520 | pRSFDuet-MpBHY | MpBHY | MpBHY-expression plasmid for production of zeinoxanthin (and zeaxanthin) when coexpressed with a plasmid to biosynthesize α-carotene (and β-carotene) in E. coli. | Takemura, M. et al. Planta 241 (3): 699-710 (2015). PMID 25467956. [link to RRC of NBRP] |
| RDB19521 | pRSFDuet-MpBHY/MpCYP97C | MpBHY, MpCYP97C | MpBHY- and MpCYP97C-expression plasmid for production of lutein (and zeaxanthin) when coexpressed with a plasmid to biosynthesize α-carotene (and β-carotene) in E. coli. | Takemura, M. et al. Planta 241 (3): 699-710 (2015). PMID 25467956. [link to RRC of NBRP] |
| RDB19522 | CDF-MpCYP97C-MpLCYe | MpCYP97C, MpLCYe | MpCYP97C- and MpLCYe-expression plasmid for production of lutein when coexpreessd with a plasmid to biosynthsize zeinoxanthin in E. coli. | Takemura, M. et al. Synth. Biol. (Oxf) 6 (1): ysab012 (2021). PMID 34712837. [link to RRC of NBRP] |
Please refer Fig. 1 of Misawa, N. et al. Methods Enzymol. 670: 87-137 (2022) for the structure of plasmids.
(GRP0075e 2023.06.26 T.M.)
2023.06.26
