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List of Binding Element


Catalog no. Type Name of clone Running title
RDB02968 DNA clone pTcf7wtluc Reporter plasmid carring TCF-binding consensus sequence
RDB02969 DNA clone pTcf7mtluc Reporter plasmid carring TCF-binding consensus sequence
RDB06776 DNA clone TP1-luc(981-6) (a.k.a. pGa981-6) Reporter construct containing the hexamerized 50 bp EBNA2 response element of the TP-1 promoter.

Our Constructed Element

37 sets of reporter element which consists of wild-type and mutated elements abut Renilla luciferase reporter gene.

Name of wild-type clone Running title Wild-type Mutant Map
forward reverse forward reverse
pAP1/PMA Reporter construct of AP1 responce element/PMA RDB03449   RDB03450 RDB03451 RDB3449.pdf
p3AP1/PMA(F) Reporter construct of AP1 responce element/PMA RDB03452 RDB03453 RDB03454 RDB03455 RDB3452.pdf
pCRE Reporter construct of CRE responce element/PMA RDB03456   RDB03457 RDB03458 RDB3456.pdf
p3CRE Reporter construct of CRE responce element/PMA RDB03459   RDB03460 RDB03461 RDB3459.pdf

Assay was done in a 24-well plate. 0.5 ug each of reporter and explression plasmids, respectivery, were used. Luciferase activity was normalized by that of wildtype (RDB03459) and mutant (RDB03460) reporter, respectively.
pDRE(F) Reporter construct of DRE responce element/PMA RDB03462 RDB03463 RDB03464 RDB03465 RDB3462.pdf
pC/EBP RE-TK hRluc(F) Reporter construct of C/EBP response element RDB05595 RDB05658 RDB05596 RDB05659 RDB5595.pdf
pCACCCBox RE-TK hRluc(F) Reporter construct of CACCCBox(EKLF) response element RDB05618 RDB05677 RDB05619   RDB5618.pdf
pCrAG RE-TK hRluc(F) Reporter construct of CrAG response element RDB05580 RDB05644 RDB05581 RDB05645 RDB5580.pdf
pE-Box RE-TK hRluc(F) Reporter construct of E-Box response element RDB05587 RDB05652 RDB05588   RDB5587.pdf
pE2F RE-TK hRluc(F) Reporter construct of E2F response element RDB05578 RDB05642 RDB05579 RDB05643 RDB5578.pdf
pEgr-1 RE-TK hRluc(F) Reporter construct of Egr-1 response element RDB05597 RDB05660 RDB05598 RDB05661 RDB5597.pdf
pER RE-TK hRluc(F) Reporter construct of ER (estrogen receptor) response element RDB05626 RDB05683 RDB05627 RDB05684 RDB5626.pdf
pEts RE-TK hRluc(F) Reporter construct of Ets response element RDB05602 RDB05665 RDB05603 RDB05666 RDB5602.pdf
pFXR RE-TK hRluc(F) Reporter construct of FXR response element RDB05636 RDB05695 RDB05637 RDB05690 RDB5636.pdf
pGAS RE-TK hRluc(F) Reporter construct of GAS(gamma-interferon activation site) response element RDB05624 RDB05681 RDB05625 RDB05682 RDB5624.pdf
pGATA RE-TK hRluc(F) Reporter construct of GATA response element RDB05599   RDB05600 RDB05662 RDB5599.pdf
pGR RE-TK hRluc(F) Reporter construct of GR (glucocorticoid receptor) response element RDB05628   RDB05629 RDB05685 RDB5628.pdf
pHRE-TK hRluc(F) Reporter construct of HRE(HIF response element) RDB05612 RDB05673   RDB05613 RDB5612.pdf
pHSF RE-TK hRluc(F) Reporter construct of HSF response element RDB05606   RDB05607 RDB05669 RDB5606.pdf
pHTLV RE-TK hRluc(F) Reporter construct of HTLV response element RDB05584 RDB05648 RDB05585 RDB05649 RDB5584.pdf
pISRE-TK hRluc(F) Reporter construct of ISRE (interferon response element) RDB05610   RDB05611 RDB05672 RDB5610.pdf
pMAREI-TK hRluc(F) Reporter construct of MAF response elementI RDB05693 RDB05670 RDB05694 RDB05671 RDB5693.pdf
pMAREII-TK hRluc Reporter construct of MAF response elementII RDB05608   RDB05609   RDB5608.pdf
pMEF2 RE-TK hRluc(F) Reporter construct of MEF2 response element RDB05692 RDB05663 RDB05601 RDB05664 RDB5692.pdf
pMyb-Box RE-TK hRluc(F) Reporter construct of Myb-Box response element RDB05591 RDB05654 RDB05592 RDB05655 RDB5591.pdf
pN-Box RE-TK hRluc(F) Reporter construct of N-Box response element RDB05589 RDB05653 RDB05590   RDB5589.pdf
pNF-kappaB RE-TK hRluc(F) Reporter construct of NF-kappaB response element RDB05576 RDB05640 RDB05577 RDB05641 RDB5576.pdf
pNFAT RE-TK hRluc(F) Reporter construct of NFAT response element RDB05604 RDB05667 RDB05605 RDB05668 RDB5604.pdf
pOCTA RE-TK hRluc(F) Reporter construct of OCTA response element RDB05586 RDB05650 RDB05691 RDB05651 RDB5586.pdf
pp53-Box RE-TK hRluc(F) Reporter construct of p53-Box response element RDB05593 RDB05656 RDB05594 RDB05657 RDB5593.pdf
pRAR RE-TK hRluc(F) Reporter construct of RAR (retinoic acid receptor) response element RDB05634 RDB05688 RDB05635 RDB05689 RDB5634.pdf
pSmad RE-TK hRluc(F) Reporter construct of Smad response element RDB05622 RDB05679 RDB05623 RDB05680 RDB5622.pdf
pSP-1 RE-TK hRluc(F) Reporter construct of SP-1 response element RDB05574 RDB05638 RDB05575 RDB05639 RDB5574.pdf
pSRE-TK hRluc(F) Reporter construct of SRE (serum response element) RDB05582 RDB05646 RDB05583 RDB05647 RDB5582.pdf
pSTAT RE-TK hRluc(F) Reporter construct of STAT response element RDB05616   RDB05617 RDB05676 RDB5616.pdf
pTCCBox RE-TK hRluc(F) Reporter construct of TCCBox(SMYD3) response element RDB05620   RDB05621 RDB05678 RDB5620.pdf
pTCF RE-TK hRluc(F) Reporter construct of TCF response element RDB05614 RDB05674 RDB05615 RDB05675 RDB5614.pdf
pTR RE-TK hRluc(F) Reporter construct of TR (thyroid hormone receptor) response element RDB05630   RDB05631 RDB05686 RDB5630.pdf
pVDR RE-TK hRluc(F) Reporter construct of VDR (vitamin D receptor) response element RDB05632   RDB05633 RDB05687 RDB5632.pdf

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2011.01.27