Gene Engineering Division – DNA BANK –

Here is a list of reporter constructs deposited by individual researchers and constructed by the Gene Engineering Division.

Clones deposited by individual researchers

• Hedgehog signal cascade
  8×3’Gli-BS-delta51-LucII (cat# RDB08061) is for monitoring the Hedgehog signal activation in mammalian cells. It has responsive elements of Gli family transcription factors. A control reporters 8xm3’Gli-BS-delta51-LucII (cat# RDB08062) harboring the mutated elements and an empty vector delta51-LucII (cat# RDB08068) are also available.
• Notch signal cascade
  pGa981-6 (cat# RDB06776) has EBNA2 response elements of the TP1 promoter. HES-1-Luc-Reporter (cat# RDB06775) has a promoter of mouse Hairy and enhancer of split 1 (HES-1). Both are suitable for monitoring the Notch signal activation in mammalian cells.
• Wnt/beta-catenin signal cascade
  pTcf7wtluc (cat# RDB02968) is for monitoring the Wnt/beta-catenin signal activation in mammalian cells. It has seven repeates of the TCF-binding consensus sequence. A control reporter pTcf7mtluc (cat# RDB02969) harboring the mutated elements is also available.

Clones constructed by the Gene Engineering Division

• We amplified upstream region of genes by PCR, cloned the amplified fragment into Renilla luciferase and firefly luciferase vectors, respectively, and then their terminal sequences were confirmed.
• Renilla luciferase collection were analysed by using 18 cell lines.
• Firefly luciferase collection were analysed by using HeLa, HepG2 and Hep3B cells.

Miscellaneous

• Higher intensity luciferase

(GRP0013e 2009.02.20 T.M.)

2023.06.08