Renilla Luciferase Construct

List of cells used

Cells	Organism	Description
B16	mouse	Skin, melanoma
C2C12	mouse	Muscle, myoblast
CHO	hamstar, Chinese	Ovary
COS-7	monkey, African green	Kidney, SV40 transformed, T antigen expressing, derived from CV-1
F9	mouse	Embryonal carcinoma
HEK293	human	Kidney, transformed with adenovirus 5 DNA
HeLa	human	Cervix, epithelial, adenocarcinoma
HepG2	human	Liver, hepatocellular carcinoma
K562	human	Erythro leukemia, bone marrow, chronic myelogenous leukemia (CML)
LNCaP	human	Prostate, metastatic site, left supraclavicular lymph nodecarcinoma
MCF-7	human	Mammary gland, brest, epithelial, metastatic site, pleural effusion adenocarcinoma
MIN7	mouse	Insulinoma (Dr. Miyazaki, Osaka Univ., Grad. Sch. Med.)
MtT/SM	rat	Pituitary (growth hormone, prolactine producing)
NEC8	human	Embryonal carcinoma, testis
NIH3T3	mouse	Normal fibroblast
PC12	rat	Adrenal gland, pheochromocytoma
SaOS-2	human	Bone, osteocarcoma
WI-38	human	Lung, fibroblast, normal

Promoter activity

-, less than 2-fold activity compared with empty pKM2L vector (RDB04026).
+, 2 to 5-fold activity.
++, 5 to 10-fold activity.
actual value, more than 10-fold activity.

CMV cytomegalovirus immediate enhancer/promoter
pKM2L-pvCMV (RDB05551)

Cell	Activity
HeLa	456.0
HepG2	205.8
B16	+++
Saos-2	+
WI38	+

SV40 simian virus 40 early enhancer/promoter region
pKM2L-pvSV40 (RDB05550)

Cell	Activity
HeLa	175.4
HepG2	70.7
COS-7	+++
C2C12	+++
PC12	++
CHO-K1	+++
LNCaP	+++
B16	+++
MCF-7	+++
MtT/SM	+++
MIN7	+++
F9	++
Saos-2	–
HEK293	++
K562	+++
WI38	–
NIH3T3	+++

HSV herpes simplex virus thymidine kinase promoter
pKM2L-pvTK (RDB05549)

Cell	Activity
HeLa	+
HepG2	+
PC12	+
B16	–
MtT/SM	++
Saos-2	–
WI38	–

AFP | alpha-fetoprotein
pKM2L-phAFP (RDB05532)

Cell	Activity
HeLa	–
HepG2	–
HepG2 +dexamethasone	++
Saos-2	–

AKR1B1 | aldose reductase
pKM2L-phAR (RDB05467)

Cell	Activity
HeLa	+
HepG2	++
B16	+
Saos-2	–
HEK293	–
WI38	–

ALOX15 | 15-lipoxygenase
pKM2L-ph15LO (RDB05533)

Cell	Activity
HeLa	–
HepG2	–
K562	+
K562 +TPA	–

ALPP | placental-type alkaline phosphatase 535aa
pKM2L-phPLAP1 (RDB05902)

Cell	Activity
HeLa	–
HepG2	–
CHO-K1	+
NEC8	–
NIH3T3	–

ALPPL2 | placental-like alkaline phosphatase 532aa
pKM2L-phALP1 (RDB05903)

Cell	Activity
HeLa	–
HepG2	–
CHO-K1	+
NEC8	372.4
Saos-2	–

B2M | beta-2 microglobulin
pKM2L-phB2M (RDB05472)

Cell	Activity
HeLa	12.2
HepG2	+
HepG2 +dexamethasone	++
HepG2 heat shock	++
C2C12	++
C2C12 +DM	–
MCF-7	11.8
K562	40.5
K562 +TPA	205.1

BMP5 | bone morphogenetic protein 5
pKM2L-phBMP5 (RDB05475)

Cell	Activity
HeLa	–
HepG2	+
COS-7	–
MCF-7	–

BMP6 | bone morphogenetic protein 6
pKM2L-phBMP6 (RDB05474)

Cell	Activity
HeLa	–
HepG2	–
PC12	+
PC12 +NGF	+
CHO-K1	–
B16	–

CCNA1 | cyclin A1
pKM2L-phCYCA1 (RDB05482)

Cell	Activity
HeLa	++
HepG2	+
NIH3T3	+
NIH3T3 serum starvation	–

CDC25A | cdc25A
pKM2L-phCDC25A (RDB05476)

Cell	Activity
HeLa	–
HepG2	+
NIH3T3	–
NIH3T3 serum starvation	–

CDC25C | cdc25c
pKM2L-phCDC25C(2) (RDB05908)

Cell	Activity
HeLa	–
HepG2	–
NIH3T3	+
NIH3T3 serum starvation	–

CDKN1A | p21 cyclin dependenk kinase inhibitor/WAF1/CIP1
pKM2L-phP21 (RDB05893)

Cell	Activity
HeLa	–
HepG2	++
NIH3T3	23.3
NIH3T3 serum starvation	13.2

CDKN1B | p27 cyclin-dependent kinase inhibitor
pKM2L-phKIP1 (RDB05508)

Cell	Activity
HeLa	+
HepG2	+
NIH3T3	+
NIH3T3 serum starvation	+

CDKN2A | p16 cyclin-dependent kinase inhibitor 2
pKM2L-phCDKN2(2) (RDB05811)

Cell	Activity
HeLa	–
HepG2	+
NIH3T3	–

CDKN2A | p14 alternative reding frame
pKM2L-phP14ARF (RDB05510)

Cell	Activity
HeLa	–
HepG2	–
COS-7	+
NIH3T3	–

CGA | glycoprotein hormone alpha-subunit
pKM2L-phGCA (RDB05524)

Cell	Activity
HeLa	–
HepG2	+
MtT/SM	16.7

CRHR1 | corticotropin releasing hormone receptor 1
pKM2L-phCRHR1 (RDB05536)

Cell	Activity
HeLa	–
HepG2	–
PC12	++
PC12 +NGF	+
MCF-7	–

CXCR4 | CXC chemokine SDF1 receptor
pKM2L-phCXCR4 (RDB05481)

Cell	Activity
HeLa	–
HepG2	+
K562	+
K562 +TPA	+

DCT | DOPAchrome tautomerase
pKM2L-phDT (RDB05492)

Cell	Activity
HeLa	–
HepG2	–
B16	15.1

E2F1 | transcription factor E2F1
pKM2L-phE2F1(1) (RDB05483)

Cell	Activity
HeLa	–
HepG2	+
NIH3T3	–
NIH3T3 serum starvation	–

E2F1 | transcription factor E2F1
pKM2L-phE2F1(2) (RDB05537)

Cell	Activity
HeLa	–
HepG2	+
NIH3T3	–
NIH3T3 serum starvation	–

EEF1A2 | elongation factor 1 A-2
pKM2L-phEF1A2 (RDB05484)

Cell	Activity
HeLa	–
HepG2	–
COS-7	–
C2C12	+
C2C12 +DM	–
PC12	11.0
PC12 +NGF	++

EGR1 | early growth response protein 1
pKM2L-phEGR1 (RDB05485)

Cell	Activity
HeLa	–
HepG2	342.1
Saos-2	++
K562	702.7
K562 +TPA	1046.0

FASLG | Fas ligand
pKM2L-phFASL (RDB05488)

Cell	Activity
HeLa	–
HepG2	–
K562	+
K562 +TPA	–

GH1 | somatomammotropin
pKM2L-phCS1 (RDB05531)

Cell	Activity
HeLa	–
HepG2	+
MtT/SM	12.0
MIN7	–
NIH3T3	–

GH1 | growth hormone
pKM2L-phGH1(2) (RDB05905)

Cell	Activity
HeLa	+
HepG2	++
MtT/SM	+

HIF1A | hypoxia-inducible factor 1 alpha subunit
pKM2L-phHIF1A (RDB05523)

Cell	Activity
HeLa	–
HepG2	+
HepG2 +CoCl2	–

HK2 | hexokinase II
pKM2L-phHKII (RDB05882)

Cell	Activity
HeLa	–
HepG2	–
C2C12	++
C2C12 +2% horse serum	++

HSP70-1 | 72-kDa heat shock protein
pKM2L-phHSP701 (RDB05541)

Cell	Activity
HeLa	10.2
HepG2	+
HepG2 heat shock	–
HEK293	10.5

ID2 | inhibitor of differentiation-2
pKM2L-phID2 (RDB05519)

Cell	Activity
HeLa	37.3
HeLa +20% FCS	43.4
HepG2	125.2
HepG2 +dexamethasone	151.0
HepG2 heat shock	13.6
COS-7	++
C2C12	++
C2C12 +DM	–
C2C12 +2% horse serum	++
PC12	33.9
PC12 +NGF	23.5
MCF-7	++
K562	65.0
K562 +TPA	44.2

IGF2 | insulin-like growth factor II,P4 promoter
pKM2L-phIGFIIP4 (RDB05528)

Cell	Activity
HeLa	–
HepG2	++
Saos-2	–

IL1B | interleukin 1 beta
pKM2L-phIL1B (RDB05526)

Cell	Activity
HeLa	–
HepG2	–
F9	–
F9 +RA	–
K562	–
K562 +TPA	22.2

INHBA | activin beta-A
pKM2L-phABA (RDB05466)

Cell	Activity
HeLa	–
HepG2	–
CHO-K1	–
NEC8	+
LNCaP	–
LNCaP +Dex	–
B16	–
MtT/SM	+
Saos-2	–
HEK293	–

INS | insulin
pKM2L-phINS (RDB05889)

Cell	Activity
HeLa	–
HepG2	–
MIN7	+

ITGA2 | integrin alpha 2 subunit
pKM2L-phIGNA2 (RDB05527)

Cell	Activity
HeLa	–
HepG2	+
COS-7	+
B16	–
MCF-7	++
F9	+
F9 +RA	++

ITGA6 | alpha 6 integrin sudunit
pKM2L-phITGA6 (RDB05469)

Cell	Activity
HeLa	–
HepG2	++
C2C12	–
C2C12 +DM	–
MCF-7	+
MCF7 +progesterone	–

ITGA6 | alpha 6 integrin sudunit
pKM2L-phITGA6(2) (RDB05912)

Cell	Activity
HeLa	–
HepG2	15.3
MCF-7	+
MCF7 +progesterone	+

ITGAV | integrin alpha v
pKM2L-phIGNA5 (RDB05518)

Cell	Activity
HeLa	–
HepG2	++
F9	17.9
F9 +RA	40.8
K562	29.1
K562 +TPA	++

ITGB3 | integrin beta-3
pKM2L-phIGNB3 (RDB05517)

Cell	Activity
HeLa	–
HepG2	–
COS-7	–
F9	–
F9 +RA	+
Saos-2	–
K562	–
K562 +TPA	+

KIT | c-kit
pKM2L-phKIT (RDB05812)

Cell	Activity
HeLa	+
HepG2	11.1
C2C12	18.2
C2C12 +2% horse serum	18.4
CHO-K1	++
B16	++

KLK3 | proatate-specific antigen/kallikrein 3
pKM2L-phPSA (RDB05546)

Cell	Activity
HeLa	–
HepG2	+
B16	–

KLK3 | proatate-specific antigen/kallikrein 3
pKM2L-phPSA(3) (RDB05910)

Cell	Activity
HeLa	+
HepG2	–
LNCaP	+
LNCaP +Dex	+
B16	+
MCF-7	–

KRT17 | keratin 17
pKM2L-phK17 (RDB05548)

Cell	Activity
HeLa	–
HepG2	–
B16	+

KRT17 | keratin 17
pKM2L-phK17(2) (RDB05907)

Cell	Activity
HeLa	–
HepG2	+
B16	33.3

KRT5 | keratin K5
pKM2L-phK5 (RDB07923)

Cell	Activity
HeLa	–
HepG2	–
B16	–
MCF-7	+

LCP1 | L-plastin
pKM2L-phLPLT (RDB05516)

Cell	Activity
HeLa	–
HepG2	+
MCF-7	–
Saos-2	–
K562	20.4
K562 +TPA	103.0

MBP | myelin basic protein (MBP)
pKM2L-phMBP (RDB05890)

Cell	Activity
HeLa	–
HepG2	–
PC12	–
PC12 +NGF	+

MC1R | melanocortin 1 receptor
pKM2L-phMC1R (RDB05515)

Cell	Activity
HeLa	–
HepG2	–
B16	–
K562	+
K562 +TPA	–

MDK | midkine
pKM2L-phMK (RDB05514)

Cell	Activity
HeLa	–
HepG2	++
CHO-K1	+
B16	++
MCF-7	++
Saos-2	–

MMP1 | collagenase-1
pKM2L-phMMP1 (RDB05478)

Cell	Activity
HeLa	–
HepG2	+
C2C12	33.2
B16	11.3
K562	++
K562 +TPA	++
NIH3T3	45.4

MMP2 | collagenase type IV
pKM2L-phCLG4 (RDB05479)

Cell	Activity
HeLa	–
HepG2	44.5
C2C12	+
C2C12 +DM	–
B16	+
MCF-7	–
Saos-2	–
NIH3T3	46.9

MSX2 | MSX2(Hox8)
pKM2L-phMSX2 (RDB05915)

Cell	Activity
HeLa	++
HepG2	42.8
CHO-K1	15.4
B16	65.3

MUC1 | breast carcinoma-associated antigen
pKM2L-phDF3 (RDB05538)

Cell	Activity
HeLa	–
HepG2	–
CHO-K1	–
MCF-7	++
Saos-2	–

MYH7 | cardiac beta-myosin heavy chain
pKM2L-phBMHC (RDB05901)

Cell	Activity
HeLa	–
HepG2	+
C2C12	–

MYL2 | Sus scrofa myosin regulatory light chain ventricular isoform
pKM2L-phMLC2V (RDB05900)

Cell	Activity
HeLa	–
HepG2	–
C2C12	+
C2C12 +2% horse serum	+

NFKBIA | I kappa B-alpha/(MAD3)
pKM2L-phIKBA (RDB05520)

Cell	Activity
HeLa	–
HeLa +TNFalpha	–
HepG2	++
F9	–
F9 +RA	–

NR4A1 | TR3 orphan receptor
pKM2L-phTR3 (RDB05884)

Cell	Activity
HeLa	–
HepG2	+
CHO-K1	10.9
NEC8	+
LNCaP	11.2
LNCaP +Dex	++

OAT | ornithine aminotransferase
pKM2L-phOAT (RDB05513)

Cell	Activity
HeLa	–
HepG2	++

P4HB | proly 4-hydroxylase beta-subunit and disulfide isomerase
pKM2L-phP4HB (RDB05511)

Cell	Activity
HeLa	+
HepG2	18.9

PAX3 | PAX3
pKM2L-phPAX3 (RDB05914)

Cell	Activity
HeLa	+
HepG2	+++
COS-7	++
B16	++

PAX6 | Paired box gene 6
pKM2L-phPAX6 (RDB05898)

Cell	Activity
HeLa	–
HepG2	–
COS-7	++
K562	++
K562 +TPA	+

PCSK2 | proprotein convertase2
pKM2L-phPC2 (RDB05885)

Cell	Activity
HeLa	–
HepG2	–
PC12	++
PC12 +NGF	+

PDK4 | pyruvate dehydrogenase kinase isoform 4
pKM2L-phPDK4 (RDB05505)

Cell	Activity
HeLa	–
HepG2	+
HepG2 +dexamethasone	+
COS-7	+
C2C12	25.5
C2C12 +DM	14.6

PLAU | urokinase plasminoden activtor
pKM2L-phUPA (RDB05547)

Cell	Activity
HeLa	–
HepG2	++
F9	+
F9 +RA	+
Saos-2	–

PPP1R12A | myosin phosphatase target subunit 1
pKM2L-phMYPT1 (RDB05535)

Cell	Activity
HeLa	++
HepG2	69.5
C2C12	24.8
C2C12 +DM	–
C2C12 +2% horse serum	28.8

PTGS2 | prostaglandin synthase-2/cyclooxygenase
pKM2L-phPGHS2(1) (RDB05480)

Cell	Activity
HeLa	+
HepG2	–
LNCaP	+
LNCaP +Dex	–
B16	+

PTGS2 | prostaglandin synthase-2/cyclooxygenase
pKM2L-phPGHS2(2) (RDB05504)

Cell	Activity
HeLa	+
HepG2	–
LNCaP	+
LNCaP +Dex	+
B16	–

PTPN1 | protein tyrosine phosphatase 1B
pKM2L-phPTP1B (RDB05493)

Cell	Activity
HeLa	–
HepG2	10.4
Saos-2	–
K562	97.0
K562 +TPA	92.9

PTPN1 | protein tyrosine phosphatase 1B
pKM2L-phPTP1B(2) (RDB05909)

Cell	Activity
HeLa	475.5
HepG2	1861.3
Saos-2	498.8
K562	1302.7
K562 +TPA	609.1

SELL | L-selectin
pKM2L-phLSEL (RDB05895)

Cell	Activity
HeLa	–
HepG2	–
K562	12.7
K562 +TPA	++

SERPINA1 | alpha-1-antitrypsin
pKM2L-phAAT (RDB05465)

Cell	Activity
HeLa	–
HepG2	388.3
HepG2 +dexamethasone	549.6
HepG2 heat shock	32.4
COS-7	–
C2C12	–
C2C12 +DM	–
PC12	–
PC12 +NGF	–
B16	–
MCF-7	–
F9	–
F9 +RA	–

SERPINE1 | plasminogen activator inhibitor 1
pKM2L-phPAI1 (RDB05506)

Cell	Activity
HeLa	–
HepG2	55.8

SLPI | leukoprotease inhibitor
pKM2L-phSLPI (RDB05500)

Cell	Activity
HeLa	+
HepG2	+
CHO-K1	++
NEC8	–

SMAD7 | Smad7
pKM2L-phSMAD7 (RDB05888)

Cell	Activity
HeLa	–
HepG2	21.2
COS-7	66.2
HEK293	81.3

SOX17 | SRY-box 17
pKM2L-phSOX17 (RDB07896)

Cell	Activity
HeLa	–
HepG2	–
COS-7	+
CHO-K1	+
F9	–
F9 +RA	–
HEK293	–

T | Brachyury/T protein
pKM2L-phBRC (RDB05896)

Cell	Activity
HeLa	–
HepG2	–
COS-7	–
K562	+
K562 +TPA	–
WI38	–

TERC | telomerase RNA
pKM2L-phTR (RDB05522)

Cell	Activity
HeLa	–
HepG2	56.4
Saos-2	–

TGFBR2 | transforming growth factor-beta type IIreceptor
pKM2L-phTGFBRII (RDB05497)

Cell	Activity
HeLa	–
HepG2	++

TP53 | p53
pKM2L-phP53 (RDB05507)

Cell	Activity
HeLa	–
HepG2	15.2

TP73 | p73 tumor protein
pKM2L-phTP73 (RDB05529)

Cell	Activity
HeLa	–
HepG2	–
C2C12	–
Saos-2	–
Saos-2 serum starvation	–
NIH3T3	+

TYR | tyrosinase Alu(-)
pKM2L-phTYR(Alu-) (RDB05494)

Cell	Activity
HeLa	–
HepG2	–
HepG2 +dexamethasone	–
HepG2 heat shock	–
C2C12	–
C2C12 +DM	–
PC12	–
PC12 +NGF	–
B16	104.6
MCF-7	–

TYRP1 | tyrosinase-related protein 1
pKM2L-phTRP1 (RDB05883)

Cell	Activity
HeLa	–
HepG2	–
B16	+

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(GRP0034e 2014.05.19 T.M.)

2023.06.08