Renilla Luciferase Construct
List of cells used
| Cells | Organism | Description |
|---|---|---|
| B16 | mouse | Skin, melanoma |
| C2C12 | mouse | Muscle, myoblast |
| CHO | hamstar, Chinese | Ovary |
| COS-7 | monkey, African green | Kidney, SV40 transformed, T antigen expressing, derived from CV-1 |
| F9 | mouse | Embryonal carcinoma |
| HEK293 | human | Kidney, transformed with adenovirus 5 DNA |
| HeLa | human | Cervix, epithelial, adenocarcinoma |
| HepG2 | human | Liver, hepatocellular carcinoma |
| K562 | human | Erythro leukemia, bone marrow, chronic myelogenous leukemia (CML) |
| LNCaP | human | Prostate, metastatic site, left supraclavicular lymph nodecarcinoma |
| MCF-7 | human | Mammary gland, brest, epithelial, metastatic site, pleural effusion adenocarcinoma |
| MIN7 | mouse | Insulinoma (Dr. Miyazaki, Osaka Univ., Grad. Sch. Med.) |
| MtT/SM | rat | Pituitary (growth hormone, prolactine producing) |
| NEC8 | human | Embryonal carcinoma, testis |
| NIH3T3 | mouse | Normal fibroblast |
| PC12 | rat | Adrenal gland, pheochromocytoma |
| SaOS-2 | human | Bone, osteocarcoma |
| WI-38 | human | Lung, fibroblast, normal |
Promoter activity
-, less than 2-fold activity compared with empty pKM2L vector (RDB04026).
+, 2 to 5-fold activity.
++, 5 to 10-fold activity.
actual value, more than 10-fold activity.
CMV cytomegalovirus immediate enhancer/promoter
pKM2L-pvCMV (RDB05551)
| Cell | Activity |
|---|---|
| HeLa | 456.0 |
| HepG2 | 205.8 |
| B16 | +++ |
| Saos-2 | + |
| WI38 | + |
SV40 simian virus 40 early enhancer/promoter region
pKM2L-pvSV40 (RDB05550)
| Cell | Activity |
|---|---|
| HeLa | 175.4 |
| HepG2 | 70.7 |
| COS-7 | +++ |
| C2C12 | +++ |
| PC12 | ++ |
| CHO-K1 | +++ |
| LNCaP | +++ |
| B16 | +++ |
| MCF-7 | +++ |
| MtT/SM | +++ |
| MIN7 | +++ |
| F9 | ++ |
| Saos-2 | – |
| HEK293 | ++ |
| K562 | +++ |
| WI38 | – |
| NIH3T3 | +++ |
HSV herpes simplex virus thymidine kinase promoter
pKM2L-pvTK (RDB05549)
| Cell | Activity |
|---|---|
| HeLa | + |
| HepG2 | + |
| PC12 | + |
| B16 | – |
| MtT/SM | ++ |
| Saos-2 | – |
| WI38 | – |
AFP | alpha-fetoprotein
pKM2L-phAFP (RDB05532)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | – |
| HepG2 +dexamethasone | ++ |
| Saos-2 | – |
AKR1B1 | aldose reductase
pKM2L-phAR (RDB05467)
| Cell | Activity |
|---|---|
| HeLa | + |
| HepG2 | ++ |
| B16 | + |
| Saos-2 | – |
| HEK293 | – |
| WI38 | – |
ALOX15 | 15-lipoxygenase
pKM2L-ph15LO (RDB05533)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | – |
| K562 | + |
| K562 +TPA | – |
ALPP | placental-type alkaline phosphatase 535aa
pKM2L-phPLAP1 (RDB05902)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | – |
| CHO-K1 | + |
| NEC8 | – |
| NIH3T3 | – |
ALPPL2 | placental-like alkaline phosphatase 532aa
pKM2L-phALP1 (RDB05903)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | – |
| CHO-K1 | + |
| NEC8 | 372.4 |
| Saos-2 | – |
B2M | beta-2 microglobulin
pKM2L-phB2M (RDB05472)
| Cell | Activity |
|---|---|
| HeLa | 12.2 |
| HepG2 | + |
| HepG2 +dexamethasone | ++ |
| HepG2 heat shock | ++ |
| C2C12 | ++ |
| C2C12 +DM | – |
| MCF-7 | 11.8 |
| K562 | 40.5 |
| K562 +TPA | 205.1 |
BMP5 | bone morphogenetic protein 5
pKM2L-phBMP5 (RDB05475)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | + |
| COS-7 | – |
| MCF-7 | – |
BMP6 | bone morphogenetic protein 6
pKM2L-phBMP6 (RDB05474)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | – |
| PC12 | + |
| PC12 +NGF | + |
| CHO-K1 | – |
| B16 | – |
CCNA1 | cyclin A1
pKM2L-phCYCA1 (RDB05482)
| Cell | Activity |
|---|---|
| HeLa | ++ |
| HepG2 | + |
| NIH3T3 | + |
| NIH3T3 serum starvation | – |
CDC25A | cdc25A
pKM2L-phCDC25A (RDB05476)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | + |
| NIH3T3 | – |
| NIH3T3 serum starvation | – |
CDC25C | cdc25c
pKM2L-phCDC25C(2) (RDB05908)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | – |
| NIH3T3 | + |
| NIH3T3 serum starvation | – |
CDKN1A | p21 cyclin dependenk kinase inhibitor/WAF1/CIP1
pKM2L-phP21 (RDB05893)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | ++ |
| NIH3T3 | 23.3 |
| NIH3T3 serum starvation | 13.2 |
CDKN1B | p27 cyclin-dependent kinase inhibitor
pKM2L-phKIP1 (RDB05508)
| Cell | Activity |
|---|---|
| HeLa | + |
| HepG2 | + |
| NIH3T3 | + |
| NIH3T3 serum starvation | + |
CDKN2A | p16 cyclin-dependent kinase inhibitor 2
pKM2L-phCDKN2(2) (RDB05811)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | + |
| NIH3T3 | – |
CDKN2A | p14 alternative reding frame
pKM2L-phP14ARF (RDB05510)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | – |
| COS-7 | + |
| NIH3T3 | – |
CGA | glycoprotein hormone alpha-subunit
pKM2L-phGCA (RDB05524)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | + |
| MtT/SM | 16.7 |
CRHR1 | corticotropin releasing hormone receptor 1
pKM2L-phCRHR1 (RDB05536)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | – |
| PC12 | ++ |
| PC12 +NGF | + |
| MCF-7 | – |
CXCR4 | CXC chemokine SDF1 receptor
pKM2L-phCXCR4 (RDB05481)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | + |
| K562 | + |
| K562 +TPA | + |
DCT | DOPAchrome tautomerase
pKM2L-phDT (RDB05492)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | – |
| B16 | 15.1 |
E2F1 | transcription factor E2F1
pKM2L-phE2F1(1) (RDB05483)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | + |
| NIH3T3 | – |
| NIH3T3 serum starvation | – |
E2F1 | transcription factor E2F1
pKM2L-phE2F1(2) (RDB05537)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | + |
| NIH3T3 | – |
| NIH3T3 serum starvation | – |
EEF1A2 | elongation factor 1 A-2
pKM2L-phEF1A2 (RDB05484)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | – |
| COS-7 | – |
| C2C12 | + |
| C2C12 +DM | – |
| PC12 | 11.0 |
| PC12 +NGF | ++ |
EGR1 | early growth response protein 1
pKM2L-phEGR1 (RDB05485)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | 342.1 |
| Saos-2 | ++ |
| K562 | 702.7 |
| K562 +TPA | 1046.0 |
FASLG | Fas ligand
pKM2L-phFASL (RDB05488)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | – |
| K562 | + |
| K562 +TPA | – |
GH1 | somatomammotropin
pKM2L-phCS1 (RDB05531)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | + |
| MtT/SM | 12.0 |
| MIN7 | – |
| NIH3T3 | – |
GH1 | growth hormone
pKM2L-phGH1(2) (RDB05905)
| Cell | Activity |
|---|---|
| HeLa | + |
| HepG2 | ++ |
| MtT/SM | + |
HIF1A | hypoxia-inducible factor 1 alpha subunit
pKM2L-phHIF1A (RDB05523)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | + |
| HepG2 +CoCl2 | – |
HK2 | hexokinase II
pKM2L-phHKII (RDB05882)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | – |
| C2C12 | ++ |
| C2C12 +2% horse serum | ++ |
HSP70-1 | 72-kDa heat shock protein
pKM2L-phHSP701 (RDB05541)
| Cell | Activity |
|---|---|
| HeLa | 10.2 |
| HepG2 | + |
| HepG2 heat shock | – |
| HEK293 | 10.5 |
ID2 | inhibitor of differentiation-2
pKM2L-phID2 (RDB05519)
| Cell | Activity |
|---|---|
| HeLa | 37.3 |
| HeLa +20% FCS | 43.4 |
| HepG2 | 125.2 |
| HepG2 +dexamethasone | 151.0 |
| HepG2 heat shock | 13.6 |
| COS-7 | ++ |
| C2C12 | ++ |
| C2C12 +DM | – |
| C2C12 +2% horse serum | ++ |
| PC12 | 33.9 |
| PC12 +NGF | 23.5 |
| MCF-7 | ++ |
| K562 | 65.0 |
| K562 +TPA | 44.2 |
IGF2 | insulin-like growth factor II,P4 promoter
pKM2L-phIGFIIP4 (RDB05528)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | ++ |
| Saos-2 | – |
IL1B | interleukin 1 beta
pKM2L-phIL1B (RDB05526)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | – |
| F9 | – |
| F9 +RA | – |
| K562 | – |
| K562 +TPA | 22.2 |
INHBA | activin beta-A
pKM2L-phABA (RDB05466)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | – |
| CHO-K1 | – |
| NEC8 | + |
| LNCaP | – |
| LNCaP +Dex | – |
| B16 | – |
| MtT/SM | + |
| Saos-2 | – |
| HEK293 | – |
INS | insulin
pKM2L-phINS (RDB05889)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | – |
| MIN7 | + |
ITGA2 | integrin alpha 2 subunit
pKM2L-phIGNA2 (RDB05527)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | + |
| COS-7 | + |
| B16 | – |
| MCF-7 | ++ |
| F9 | + |
| F9 +RA | ++ |
ITGA6 | alpha 6 integrin sudunit
pKM2L-phITGA6 (RDB05469)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | ++ |
| C2C12 | – |
| C2C12 +DM | – |
| MCF-7 | + |
| MCF7 +progesterone | – |
ITGA6 | alpha 6 integrin sudunit
pKM2L-phITGA6(2) (RDB05912)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | 15.3 |
| MCF-7 | + |
| MCF7 +progesterone | + |
ITGAV | integrin alpha v
pKM2L-phIGNA5 (RDB05518)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | ++ |
| F9 | 17.9 |
| F9 +RA | 40.8 |
| K562 | 29.1 |
| K562 +TPA | ++ |
ITGB3 | integrin beta-3
pKM2L-phIGNB3 (RDB05517)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | – |
| COS-7 | – |
| F9 | – |
| F9 +RA | + |
| Saos-2 | – |
| K562 | – |
| K562 +TPA | + |
KIT | c-kit
pKM2L-phKIT (RDB05812)
| Cell | Activity |
|---|---|
| HeLa | + |
| HepG2 | 11.1 |
| C2C12 | 18.2 |
| C2C12 +2% horse serum | 18.4 |
| CHO-K1 | ++ |
| B16 | ++ |
KLK3 | proatate-specific antigen/kallikrein 3
pKM2L-phPSA (RDB05546)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | + |
| B16 | – |
KLK3 | proatate-specific antigen/kallikrein 3
pKM2L-phPSA(3) (RDB05910)
| Cell | Activity |
|---|---|
| HeLa | + |
| HepG2 | – |
| LNCaP | + |
| LNCaP +Dex | + |
| B16 | + |
| MCF-7 | – |
KRT17 | keratin 17
pKM2L-phK17 (RDB05548)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | – |
| B16 | + |
KRT17 | keratin 17
pKM2L-phK17(2) (RDB05907)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | + |
| B16 | 33.3 |
KRT5 | keratin K5
pKM2L-phK5 (RDB07923)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | – |
| B16 | – |
| MCF-7 | + |
LCP1 | L-plastin
pKM2L-phLPLT (RDB05516)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | + |
| MCF-7 | – |
| Saos-2 | – |
| K562 | 20.4 |
| K562 +TPA | 103.0 |
MBP | myelin basic protein (MBP)
pKM2L-phMBP (RDB05890)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | – |
| PC12 | – |
| PC12 +NGF | + |
MC1R | melanocortin 1 receptor
pKM2L-phMC1R (RDB05515)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | – |
| B16 | – |
| K562 | + |
| K562 +TPA | – |
MDK | midkine
pKM2L-phMK (RDB05514)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | ++ |
| CHO-K1 | + |
| B16 | ++ |
| MCF-7 | ++ |
| Saos-2 | – |
MMP1 | collagenase-1
pKM2L-phMMP1 (RDB05478)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | + |
| C2C12 | 33.2 |
| B16 | 11.3 |
| K562 | ++ |
| K562 +TPA | ++ |
| NIH3T3 | 45.4 |
MMP2 | collagenase type IV
pKM2L-phCLG4 (RDB05479)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | 44.5 |
| C2C12 | + |
| C2C12 +DM | – |
| B16 | + |
| MCF-7 | – |
| Saos-2 | – |
| NIH3T3 | 46.9 |
MSX2 | MSX2(Hox8)
pKM2L-phMSX2 (RDB05915)
| Cell | Activity |
|---|---|
| HeLa | ++ |
| HepG2 | 42.8 |
| CHO-K1 | 15.4 |
| B16 | 65.3 |
MUC1 | breast carcinoma-associated antigen
pKM2L-phDF3 (RDB05538)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | – |
| CHO-K1 | – |
| MCF-7 | ++ |
| Saos-2 | – |
MYH7 | cardiac beta-myosin heavy chain
pKM2L-phBMHC (RDB05901)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | + |
| C2C12 | – |
MYL2 | Sus scrofa myosin regulatory light chain ventricular isoform
pKM2L-phMLC2V (RDB05900)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | – |
| C2C12 | + |
| C2C12 +2% horse serum | + |
NFKBIA | I kappa B-alpha/(MAD3)
pKM2L-phIKBA (RDB05520)
| Cell | Activity |
|---|---|
| HeLa | – |
| HeLa +TNFalpha | – |
| HepG2 | ++ |
| F9 | – |
| F9 +RA | – |
NR4A1 | TR3 orphan receptor
pKM2L-phTR3 (RDB05884)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | + |
| CHO-K1 | 10.9 |
| NEC8 | + |
| LNCaP | 11.2 |
| LNCaP +Dex | ++ |
OAT | ornithine aminotransferase
pKM2L-phOAT (RDB05513)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | ++ |
P4HB | proly 4-hydroxylase beta-subunit and disulfide isomerase
pKM2L-phP4HB (RDB05511)
| Cell | Activity |
|---|---|
| HeLa | + |
| HepG2 | 18.9 |
PAX3 | PAX3
pKM2L-phPAX3 (RDB05914)
| Cell | Activity |
|---|---|
| HeLa | + |
| HepG2 | +++ |
| COS-7 | ++ |
| B16 | ++ |
PAX6 | Paired box gene 6
pKM2L-phPAX6 (RDB05898)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | – |
| COS-7 | ++ |
| K562 | ++ |
| K562 +TPA | + |
PCSK2 | proprotein convertase2
pKM2L-phPC2 (RDB05885)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | – |
| PC12 | ++ |
| PC12 +NGF | + |
PDK4 | pyruvate dehydrogenase kinase isoform 4
pKM2L-phPDK4 (RDB05505)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | + |
| HepG2 +dexamethasone | + |
| COS-7 | + |
| C2C12 | 25.5 |
| C2C12 +DM | 14.6 |
PLAU | urokinase plasminoden activtor
pKM2L-phUPA (RDB05547)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | ++ |
| F9 | + |
| F9 +RA | + |
| Saos-2 | – |
PPP1R12A | myosin phosphatase target subunit 1
pKM2L-phMYPT1 (RDB05535)
| Cell | Activity |
|---|---|
| HeLa | ++ |
| HepG2 | 69.5 |
| C2C12 | 24.8 |
| C2C12 +DM | – |
| C2C12 +2% horse serum | 28.8 |
PTGS2 | prostaglandin synthase-2/cyclooxygenase
pKM2L-phPGHS2(1) (RDB05480)
| Cell | Activity |
|---|---|
| HeLa | + |
| HepG2 | – |
| LNCaP | + |
| LNCaP +Dex | – |
| B16 | + |
PTGS2 | prostaglandin synthase-2/cyclooxygenase
pKM2L-phPGHS2(2) (RDB05504)
| Cell | Activity |
|---|---|
| HeLa | + |
| HepG2 | – |
| LNCaP | + |
| LNCaP +Dex | + |
| B16 | – |
PTPN1 | protein tyrosine phosphatase 1B
pKM2L-phPTP1B (RDB05493)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | 10.4 |
| Saos-2 | – |
| K562 | 97.0 |
| K562 +TPA | 92.9 |
PTPN1 | protein tyrosine phosphatase 1B
pKM2L-phPTP1B(2) (RDB05909)
| Cell | Activity |
|---|---|
| HeLa | 475.5 |
| HepG2 | 1861.3 |
| Saos-2 | 498.8 |
| K562 | 1302.7 |
| K562 +TPA | 609.1 |
SELL | L-selectin
pKM2L-phLSEL (RDB05895)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | – |
| K562 | 12.7 |
| K562 +TPA | ++ |
SERPINA1 | alpha-1-antitrypsin
pKM2L-phAAT (RDB05465)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | 388.3 |
| HepG2 +dexamethasone | 549.6 |
| HepG2 heat shock | 32.4 |
| COS-7 | – |
| C2C12 | – |
| C2C12 +DM | – |
| PC12 | – |
| PC12 +NGF | – |
| B16 | – |
| MCF-7 | – |
| F9 | – |
| F9 +RA | – |
SERPINE1 | plasminogen activator inhibitor 1
pKM2L-phPAI1 (RDB05506)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | 55.8 |
SLPI | leukoprotease inhibitor
pKM2L-phSLPI (RDB05500)
| Cell | Activity |
|---|---|
| HeLa | + |
| HepG2 | + |
| CHO-K1 | ++ |
| NEC8 | – |
SMAD7 | Smad7
pKM2L-phSMAD7 (RDB05888)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | 21.2 |
| COS-7 | 66.2 |
| HEK293 | 81.3 |
SOX17 | SRY-box 17
pKM2L-phSOX17 (RDB07896)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | – |
| COS-7 | + |
| CHO-K1 | + |
| F9 | – |
| F9 +RA | – |
| HEK293 | – |
T | Brachyury/T protein
pKM2L-phBRC (RDB05896)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | – |
| COS-7 | – |
| K562 | + |
| K562 +TPA | – |
| WI38 | – |
TERC | telomerase RNA
pKM2L-phTR (RDB05522)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | 56.4 |
| Saos-2 | – |
TGFBR2 | transforming growth factor-beta type IIreceptor
pKM2L-phTGFBRII (RDB05497)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | ++ |
TP53 | p53
pKM2L-phP53 (RDB05507)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | 15.2 |
TP73 | p73 tumor protein
pKM2L-phTP73 (RDB05529)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | – |
| C2C12 | – |
| Saos-2 | – |
| Saos-2 serum starvation | – |
| NIH3T3 | + |
TYR | tyrosinase Alu(-)
pKM2L-phTYR(Alu-) (RDB05494)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | – |
| HepG2 +dexamethasone | – |
| HepG2 heat shock | – |
| C2C12 | – |
| C2C12 +DM | – |
| PC12 | – |
| PC12 +NGF | – |
| B16 | 104.6 |
| MCF-7 | – |
TYRP1 | tyrosinase-related protein 1
pKM2L-phTRP1 (RDB05883)
| Cell | Activity |
|---|---|
| HeLa | – |
| HepG2 | – |
| B16 | + |
go to Head(GRP0034e 2014.05.19 T.M.)
2023.06.08
