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Clontech Laboratories, Inc. and RIKEN BioResource Research Center concluded license agreement on preservation and distribution of Fluorescent Proteins, DsRed2 and mCherry for academic use

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Renilla Luciferase Construct


Renilla Luciferase ConstructRenilla

List of cells used

Cells Organism Description
B16 mouse Skin, melanoma
C2C12 mouse Muscle, myoblast
CHO hamstar, Chinese Ovary
COS-7 monkey, African green Kidney, SV40 transformed, T antigen expressing, derived from CV-1
F9 mouse Embryonal carcinoma
HEK293 human Kidney, transformed with adenovirus 5 DNA
HeLa human Cervix, epithelial, adenocarcinoma
HepG2 human Liver, hepatocellular carcinoma
K562 human Erythro leukemia, bone marrow, chronic myelogenous leukemia (CML)
LNCaP human Prostate, metastatic site, left supraclavicular lymph nodecarcinoma
MCF-7 human Mammary gland, brest, epithelial, metastatic site, pleural effusion adenocarcinoma
MIN7 mouse Insulinoma (Dr. Miyazaki, Osaka Univ., Grad. Sch. Med.)
MtT/SM rat Pituitary (growth hormone, prolactine producing)
NEC8 human Embryonal carcinoma, testis
NIH3T3 mouse Normal fibroblast
PC12 rat Adrenal gland, pheochromocytoma
SaOS-2 human Bone, osteocarcoma
WI-38 human Lung, fibroblast, normal

Promoter activity

-, less than 2-fold activity compared with empty pKM2L vector (RDB04026).
+, 2 to 5-fold activity.
++, 5 to 10-fold activity.
actual value, more than 10-fold activity.

CMV cytomegalovirus immediate enhancer/promoter
pKM2L-pvCMV (RDB05551)

Cell Activity
HeLa 456.0
HepG2 205.8
B16 +++
Saos-2 +
WI38 +

SV40 simian virus 40 early enhancer/promoter region
pKM2L-pvSV40 (RDB05550)

Cell Activity
HeLa 175.4
HepG2 70.7
COS-7 +++
C2C12 +++
PC12 ++
CHO-K1 +++
LNCaP +++
B16 +++
MCF-7 +++
MtT/SM +++
MIN7 +++
F9 ++
Saos-2
HEK293 ++
K562 +++
WI38
NIH3T3 +++

HSV herpes simplex virus thymidine kinase promoter
pKM2L-pvTK (RDB05549)

Cell Activity
HeLa +
HepG2 +
PC12 +
B16
MtT/SM ++
Saos-2
WI38

AFP | alpha-fetoprotein
pKM2L-phAFP (RDB05532)

Cell Activity
HeLa
HepG2
HepG2 +dexamethasone ++
Saos-2

AKR1B1 | aldose reductase
pKM2L-phAR (RDB05467)

Cell Activity
HeLa +
HepG2 ++
B16 +
Saos-2
HEK293
WI38

ALOX15 | 15-lipoxygenase
pKM2L-ph15LO (RDB05533)

Cell Activity
HeLa
HepG2
K562 +
K562 +TPA

ALPP | placental-type alkaline phosphatase 535aa
pKM2L-phPLAP1 (RDB05902)

Cell Activity
HeLa
HepG2
CHO-K1 +
NEC8
NIH3T3

ALPPL2 | placental-like alkaline phosphatase 532aa
pKM2L-phALP1 (RDB05903)

Cell Activity
HeLa
HepG2
CHO-K1 +
NEC8 372.4
Saos-2

B2M | beta-2 microglobulin
pKM2L-phB2M (RDB05472)

Cell Activity
HeLa 12.2
HepG2 +
HepG2 +dexamethasone ++
HepG2 heat shock ++
C2C12 ++
C2C12 +DM
MCF-7 11.8
K562 40.5
K562 +TPA 205.1

BMP5 | bone morphogenetic protein 5
pKM2L-phBMP5 (RDB05475)

Cell Activity
HeLa
HepG2 +
COS-7
MCF-7

BMP6 | bone morphogenetic protein 6
pKM2L-phBMP6 (RDB05474)

Cell Activity
HeLa
HepG2
PC12 +
PC12 +NGF +
CHO-K1
B16

CCNA1 | cyclin A1
pKM2L-phCYCA1 (RDB05482)

Cell Activity
HeLa ++
HepG2 +
NIH3T3 +
NIH3T3 serum starvation

CDC25A | cdc25A
pKM2L-phCDC25A (RDB05476)

Cell Activity
HeLa
HepG2 +
NIH3T3
NIH3T3 serum starvation

CDC25C | cdc25c
pKM2L-phCDC25C(2) (RDB05908)

Cell Activity
HeLa
HepG2
NIH3T3 +
NIH3T3 serum starvation

CDKN1A | p21 cyclin dependenk kinase inhibitor/WAF1/CIP1
pKM2L-phP21 (RDB05893)

Cell Activity
HeLa
HepG2 ++
NIH3T3 23.3
NIH3T3 serum starvation 13.2

CDKN1B | p27 cyclin-dependent kinase inhibitor
pKM2L-phKIP1 (RDB05508)

Cell Activity
HeLa +
HepG2 +
NIH3T3 +
NIH3T3 serum starvation +

CDKN2A | p16 cyclin-dependent kinase inhibitor 2
pKM2L-phCDKN2(2) (RDB05811)

Cell Activity
HeLa
HepG2 +
NIH3T3

CDKN2A | p14 alternative reding frame
pKM2L-phP14ARF (RDB05510)

Cell Activity
HeLa
HepG2
COS-7 +
NIH3T3

CGA | glycoprotein hormone alpha-subunit
pKM2L-phGCA (RDB05524)

Cell Activity
HeLa
HepG2 +
MtT/SM 16.7

CRHR1 | corticotropin releasing hormone receptor 1
pKM2L-phCRHR1 (RDB05536)

Cell Activity
HeLa
HepG2
PC12 ++
PC12 +NGF +
MCF-7

CXCR4 | CXC chemokine SDF1 receptor
pKM2L-phCXCR4 (RDB05481)

Cell Activity
HeLa
HepG2 +
K562 +
K562 +TPA +

DCT | DOPAchrome tautomerase
pKM2L-phDT (RDB05492)

Cell Activity
HeLa
HepG2
B16 15.1

E2F1 | transcription factor E2F1
pKM2L-phE2F1(1) (RDB05483)

Cell Activity
HeLa
HepG2 +
NIH3T3
NIH3T3 serum starvation

E2F1 | transcription factor E2F1
pKM2L-phE2F1(2) (RDB05537)

Cell Activity
HeLa
HepG2 +
NIH3T3
NIH3T3 serum starvation

EEF1A2 | elongation factor 1 A-2
pKM2L-phEF1A2 (RDB05484)

Cell Activity
HeLa
HepG2
COS-7
C2C12 +
C2C12 +DM
PC12 11.0
PC12 +NGF ++

EGR1 | early growth response protein 1
pKM2L-phEGR1 (RDB05485)

Cell Activity
HeLa
HepG2 342.1
Saos-2 ++
K562 702.7
K562 +TPA 1046.0

FASLG | Fas ligand
pKM2L-phFASL (RDB05488)

Cell Activity
HeLa
HepG2
K562 +
K562 +TPA

GH1 | somatomammotropin
pKM2L-phCS1 (RDB05531)

Cell Activity
HeLa
HepG2 +
MtT/SM 12.0
MIN7
NIH3T3

GH1 | growth hormone
pKM2L-phGH1(2) (RDB05905)

Cell Activity
HeLa +
HepG2 ++
MtT/SM +

HIF1A | hypoxia-inducible factor 1 alpha subunit
pKM2L-phHIF1A (RDB05523)

Cell Activity
HeLa
HepG2 +
HepG2 +CoCl2

HK2 | hexokinase II
pKM2L-phHKII (RDB05882)

Cell Activity
HeLa
HepG2
C2C12 ++
C2C12 +2% horse serum ++

HSP70-1 | 72-kDa heat shock protein
pKM2L-phHSP701 (RDB05541)

Cell Activity
HeLa 10.2
HepG2 +
HepG2 heat shock
HEK293 10.5

ID2 | inhibitor of differentiation-2
pKM2L-phID2 (RDB05519)

Cell Activity
HeLa 37.3
HeLa +20% FCS 43.4
HepG2 125.2
HepG2 +dexamethasone 151.0
HepG2 heat shock 13.6
COS-7 ++
C2C12 ++
C2C12 +DM
C2C12 +2% horse serum ++
PC12 33.9
PC12 +NGF 23.5
MCF-7 ++
K562 65.0
K562 +TPA 44.2

IGF2 | insulin-like growth factor II,P4 promoter
pKM2L-phIGFIIP4 (RDB05528)

Cell Activity
HeLa
HepG2 ++
Saos-2

IL1B | interleukin 1 beta
pKM2L-phIL1B (RDB05526)

Cell Activity
HeLa
HepG2
F9
F9 +RA
K562
K562 +TPA 22.2

INHBA | activin beta-A
pKM2L-phABA (RDB05466)

Cell Activity
HeLa
HepG2
CHO-K1
NEC8 +
LNCaP
LNCaP +Dex
B16
MtT/SM +
Saos-2
HEK293

INS | insulin
pKM2L-phINS (RDB05889)

Cell Activity
HeLa
HepG2
MIN7 +

ITGA2 | integrin alpha 2 subunit
pKM2L-phIGNA2 (RDB05527)

Cell Activity
HeLa
HepG2 +
COS-7 +
B16
MCF-7 ++
F9 +
F9 +RA ++

ITGA6 | alpha 6 integrin sudunit
pKM2L-phITGA6 (RDB05469)

Cell Activity
HeLa
HepG2 ++
C2C12
C2C12 +DM
MCF-7 +
MCF7 +progesterone

ITGA6 | alpha 6 integrin sudunit
pKM2L-phITGA6(2) (RDB05912)

Cell Activity
HeLa
HepG2 15.3
MCF-7 +
MCF7 +progesterone +

ITGAV | integrin alpha v
pKM2L-phIGNA5 (RDB05518)

Cell Activity
HeLa
HepG2 ++
F9 17.9
F9 +RA 40.8
K562 29.1
K562 +TPA ++

ITGB3 | integrin beta-3
pKM2L-phIGNB3 (RDB05517)

Cell Activity
HeLa
HepG2
COS-7
F9
F9 +RA +
Saos-2
K562
K562 +TPA +

KIT | c-kit
pKM2L-phKIT (RDB05812)

Cell Activity
HeLa +
HepG2 11.1
C2C12 18.2
C2C12 +2% horse serum 18.4
CHO-K1 ++
B16 ++

KLK3 | proatate-specific antigen/kallikrein 3
pKM2L-phPSA (RDB05546)

Cell Activity
HeLa
HepG2 +
B16

KLK3 | proatate-specific antigen/kallikrein 3
pKM2L-phPSA(3) (RDB05910)

Cell Activity
HeLa +
HepG2
LNCaP +
LNCaP +Dex +
B16 +
MCF-7

KRT17 | keratin 17
pKM2L-phK17 (RDB05548)

Cell Activity
HeLa
HepG2
B16 +

KRT17 | keratin 17
pKM2L-phK17(2) (RDB05907)

Cell Activity
HeLa
HepG2 +
B16 33.3

KRT5 | keratin K5
pKM2L-phK5 (RDB07923)

Cell Activity
HeLa
HepG2
B16
MCF-7 +

LCP1 | L-plastin
pKM2L-phLPLT (RDB05516)

Cell Activity
HeLa
HepG2 +
MCF-7
Saos-2
K562 20.4
K562 +TPA 103.0

MBP | myelin basic protein (MBP)
pKM2L-phMBP (RDB05890)

Cell Activity
HeLa
HepG2
PC12
PC12 +NGF +

MC1R | melanocortin 1 receptor
pKM2L-phMC1R (RDB05515)

Cell Activity
HeLa
HepG2
B16
K562 +
K562 +TPA

MDK | midkine
pKM2L-phMK (RDB05514)

Cell Activity
HeLa
HepG2 ++
CHO-K1 +
B16 ++
MCF-7 ++
Saos-2

MMP1 | collagenase-1
pKM2L-phMMP1 (RDB05478)

Cell Activity
HeLa
HepG2 +
C2C12 33.2
B16 11.3
K562 ++
K562 +TPA ++
NIH3T3 45.4

MMP2 | collagenase type IV
pKM2L-phCLG4 (RDB05479)

Cell Activity
HeLa
HepG2 44.5
C2C12 +
C2C12 +DM
B16 +
MCF-7
Saos-2
NIH3T3 46.9

MSX2 | MSX2(Hox8)
pKM2L-phMSX2 (RDB05915)

Cell Activity
HeLa ++
HepG2 42.8
CHO-K1 15.4
B16 65.3

MUC1 | breast carcinoma-associated antigen
pKM2L-phDF3 (RDB05538)

Cell Activity
HeLa
HepG2
CHO-K1
MCF-7 ++
Saos-2

MYH7 | cardiac beta-myosin heavy chain
pKM2L-phBMHC (RDB05901)

Cell Activity
HeLa
HepG2 +
C2C12

MYL2 | Sus scrofa myosin regulatory light chain ventricular isoform
pKM2L-phMLC2V (RDB05900)

Cell Activity
HeLa
HepG2
C2C12 +
C2C12 +2% horse serum +

NFKBIA | I kappa B-alpha/(MAD3)
pKM2L-phIKBA (RDB05520)

Cell Activity
HeLa
HeLa +TNFalpha
HepG2 ++
F9
F9 +RA

NR4A1 | TR3 orphan receptor
pKM2L-phTR3 (RDB05884)

Cell Activity
HeLa
HepG2 +
CHO-K1 10.9
NEC8 +
LNCaP 11.2
LNCaP +Dex ++

OAT | ornithine aminotransferase
pKM2L-phOAT (RDB05513)

Cell Activity
HeLa
HepG2 ++

P4HB | proly 4-hydroxylase beta-subunit and disulfide isomerase
pKM2L-phP4HB (RDB05511)

Cell Activity
HeLa +
HepG2 18.9

PAX3 | PAX3
pKM2L-phPAX3 (RDB05914)

Cell Activity
HeLa +
HepG2 +++
COS-7 ++
B16 ++

PAX6 | Paired box gene 6
pKM2L-phPAX6 (RDB05898)

Cell Activity
HeLa
HepG2
COS-7 ++
K562 ++
K562 +TPA +

PCSK2 | proprotein convertase2
pKM2L-phPC2 (RDB05885)

Cell Activity
HeLa
HepG2
PC12 ++
PC12 +NGF +

PDK4 | pyruvate dehydrogenase kinase isoform 4
pKM2L-phPDK4 (RDB05505)

Cell Activity
HeLa
HepG2 +
HepG2 +dexamethasone +
COS-7 +
C2C12 25.5
C2C12 +DM 14.6

PLAU | urokinase plasminoden activtor
pKM2L-phUPA (RDB05547)

Cell Activity
HeLa
HepG2 ++
F9 +
F9 +RA +
Saos-2

PPP1R12A | myosin phosphatase target subunit 1
pKM2L-phMYPT1 (RDB05535)

Cell Activity
HeLa ++
HepG2 69.5
C2C12 24.8
C2C12 +DM
C2C12 +2% horse serum 28.8

PTGS2 | prostaglandin synthase-2/cyclooxygenase
pKM2L-phPGHS2(1) (RDB05480)

Cell Activity
HeLa +
HepG2
LNCaP +
LNCaP +Dex
B16 +

PTGS2 | prostaglandin synthase-2/cyclooxygenase
pKM2L-phPGHS2(2) (RDB05504)

Cell Activity
HeLa +
HepG2
LNCaP +
LNCaP +Dex +
B16

PTPN1 | protein tyrosine phosphatase 1B
pKM2L-phPTP1B (RDB05493)

Cell Activity
HeLa
HepG2 10.4
Saos-2
K562 97.0
K562 +TPA 92.9

PTPN1 | protein tyrosine phosphatase 1B
pKM2L-phPTP1B(2) (RDB05909)

Cell Activity
HeLa 475.5
HepG2 1861.3
Saos-2 498.8
K562 1302.7
K562 +TPA 609.1

SELL | L-selectin
pKM2L-phLSEL (RDB05895)

Cell Activity
HeLa
HepG2
K562 12.7
K562 +TPA ++

SERPINA1 | alpha-1-antitrypsin
pKM2L-phAAT (RDB05465)

Cell Activity
HeLa
HepG2 388.3
HepG2 +dexamethasone 549.6
HepG2 heat shock 32.4
COS-7
C2C12
C2C12 +DM
PC12
PC12 +NGF
B16
MCF-7
F9
F9 +RA

SERPINE1 | plasminogen activator inhibitor 1
pKM2L-phPAI1 (RDB05506)

Cell Activity
HeLa
HepG2 55.8

SLPI | leukoprotease inhibitor
pKM2L-phSLPI (RDB05500)

Cell Activity
HeLa +
HepG2 +
CHO-K1 ++
NEC8

SMAD7 | Smad7
pKM2L-phSMAD7 (RDB05888)

Cell Activity
HeLa
HepG2 21.2
COS-7 66.2
HEK293 81.3

SOX17 | SRY-box 17
pKM2L-phSOX17 (RDB07896)

Cell Activity
HeLa
HepG2
COS-7 +
CHO-K1 +
F9
F9 +RA
HEK293

T | Brachyury/T protein
pKM2L-phBRC (RDB05896)

Cell Activity
HeLa
HepG2
COS-7
K562 +
K562 +TPA
WI38

TERC | telomerase RNA
pKM2L-phTR (RDB05522)

Cell Activity
HeLa
HepG2 56.4
Saos-2

TGFBR2 | transforming growth factor-beta type IIreceptor
pKM2L-phTGFBRII (RDB05497)

Cell Activity
HeLa
HepG2 ++

TP53 | p53
pKM2L-phP53 (RDB05507)

Cell Activity
HeLa
HepG2 15.2

TP73 | p73 tumor protein
pKM2L-phTP73 (RDB05529)

Cell Activity
HeLa
HepG2
C2C12
Saos-2
Saos-2 serum starvation
NIH3T3 +

TYR | tyrosinase Alu(-)
pKM2L-phTYR(Alu-) (RDB05494)

Cell Activity
HeLa
HepG2
HepG2 +dexamethasone
HepG2 heat shock
C2C12
C2C12 +DM
PC12
PC12 +NGF
B16 104.6
MCF-7

TYRP1 | tyrosinase-related protein 1
pKM2L-phTRP1 (RDB05883)

Cell Activity
HeLa
HepG2
B16 +

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(GRP0034e 2014.05.19 T.M.)

2019.02.02