BRC RESOURCE NEWS

BRC RESOURCE NEWS

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Mammalian Expression Vector, pEF-BOS series (2022/03/19)

  • pEF-BOS and pEF-BOS-EX are mammalian expression vectors developed by Dr. Nagata Shigekazu of Immunology Frontier Research Center, Osaka University (Mizushima, S and Nagata, S. Nucleic Acids Res. 18 (17): 5322, 1990. Murai, K. et al. Proc. Natl. Acad. Sci. USA. 95 (7): 3461-3466, 1998). These vectors carry the human elongation factor one alpha promoter (EF-1 alpha) with high promoter activity independent of cell type and are used in gene transfer experiments into various cell types.
  • We will now provide the new modified pEF-BOS-EX vectors deposited by Dr. Nagata. pEF-Flag-EX (RDB18972) is utilized to express gene products with a FLAG-tag. pNEF-BOS-EX (RDB18970) and pPEF-BOS-EX (RDB18971) with neomycin and puromycin resistance markers, respectively, are utilized to establish stable expression cell lines. We are looking forward to receiving your request for pEF-BOS vector series.
    Cat.# Resource name Remarks
    RDB07939 pEF-BOS  
    RDB07940 pEF-BOS-EX  
    RDB18972 pEF-Flag-EX N-terminal FLAG-tag
    RDB18970 pNEF-BOS-EX Selectable markers in mammalian cells, Neomycin
    RDB18971 pPEF-BOS-EX Selectable markers in mammalian cells, Puromycin

     


(GRP0071e 2022.03.19 N.N)

2022.03.19



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