BRC RESOURCE NEWS
Mammalian Expression Vector, pEF-BOS series (2022/03/19)
- pEF-BOS and pEF-BOS-EX are mammalian expression vectors developed by Dr. Nagata Shigekazu of Immunology Frontier Research Center, Osaka University (Mizushima, S and Nagata, S. Nucleic Acids Res. 18 (17): 5322, 1990. Murai, K. et al. Proc. Natl. Acad. Sci. USA. 95 (7): 3461-3466, 1998). These vectors carry the human elongation factor one alpha promoter (EF-1 alpha) with high promoter activity independent of cell type and are used in gene transfer experiments into various cell types.
- We will now provide the new modified pEF-BOS-EX vectors deposited by Dr. Nagata. pEF-Flag-EX (RDB18972) is utilized to express gene products with a FLAG-tag. pNEF-BOS-EX (RDB18970) and pPEF-BOS-EX (RDB18971) with neomycin and puromycin resistance markers, respectively, are utilized to establish stable expression cell lines. We are looking forward to receiving your request for pEF-BOS vector series.
Cat.# Resource name Remarks RDB07939 pEF-BOS RDB07940 pEF-BOS-EX RDB18972 pEF-Flag-EX N-terminal FLAG-tag RDB18970 pNEF-BOS-EX Selectable markers in mammalian cells, Neomycin RDB18971 pPEF-BOS-EX Selectable markers in mammalian cells, Puromycin
(GRP0071e 2022.03.19 N.N)