Fucci – Fluorescent Ubiquitination-based Cell Cycle Indicator
Mouse lines
RIKEN BRC Experimental Animal Division (Search Fucci mouse strains)
| B6;129-Gt(ROSA)26Sor<tm1(Fucci2aR)Jkn> (RBRC 06511) | Mort, R.L. et al., 2014 |
| B6.Cg-Tg(FucciS/G2/M)#474Bsi (RBRC02704) B6.Cg-Tg(FucciG1)#610Bsi (RBRC02708) |
Sakaue-Sawano, A. et al., 2013. |
| B6.Cg-Tg(FucciS/G2/M)#492Bsi (RBRC02705) B6.Cg-Tg(FucciG1)#639Bsi (RBRC02709) |
Aiba, Y, et al., 2010 |
| B6.Cg-Tg (FucciS/G2/M) #504Bsi (RBRC02706) B6.Cg-Tg (FucciG1) #596Bsi (RBRC02707) |
Sakaue-Sawano, A. et al., 2008a |
Cell lines
RIKEN BRC Cell Bank (Search Fucci cells)
| HeLa/Fucci(SA)5#16 (RCB4917) HeLa/Fucci(CA)5#16 (RCB4919) |
Ando, R. et al., 2020 |
| HeLa/Fucci(CA)2 (RCB4676) NMuMG/Fucci(CA)2.1 (RCB4675) |
Sakaue-Sawano, A. et al., 2017 |
| HeLa/Fucci2 (RCB2867) NMuMG/Fucci2 (RCB2868) |
Sakaue-Sawano, A. et al., 2011 |
| HeLa/Fucci (RCB2812) NMuMG/Fucci (RCB2813) COS/Fucci (RCB2814) |
Sakaue-Sawano, A. et al., 2008a |
DNA materials
Plamid clones
| Fucci5 tFucci(SA)5/pCS2 (RDB18220) tFucci(CA)5/pCS2 (RDB18221) |
Ando, R. et al., 2020 |
|
pCDNA5-Frt-CAG-Arl13bCerulean-Fucci2a (RDB16057) pRosa26-CAG-floxNeo-Arl13bCerulean-Fucci2a (RDB16058) pCAG-Arl13bCerulean-Fucci2a (RDB16059) |
Ford, M.J. et al., 2018 |
| mCherry-hCdt1(1/100)Cy(-)/pcDNA3 (RDB15459) | Sakaue-Sawano, A. et al., 2017 |
|
pCAG-Fucci2a (RDB13080) pROSA-floxNeo-Fucci2a (RDB13081) pCDNA5-Frt-Fucci2a (RDB16056) |
Mort, R.L. et al., 2014 |
| mCherry-hCdt1(30/120)/pcDNA3 (RDB15457) mVenus-hGeminin(1/110)/pcDNA3 (RDB15458) |
Sakaue-Sawano, A. et al., 2011 |
| Tol2 plasmid mCherry-zCdt1(1/190) / pT2KXIGdeltain (RDB15278) mVenus-zGeminin (1/100) / pT2KXIGdeltain (RDB15279) mKO2-zCdt1(1/190) / pT2KXIGdeltain (RDB15275) mAG-zGeminin(1/100) / pT2KXIGdeltain (RDB15276) mAG-hGeminin (1/60) / pT2KXIGdeltain (RDB15277) |
Sugiyama, M. et al., 2009 |
Lentiviral vectors
Cell cycle indicator Fucci(CA) and Fucci(SA)
Sakaue-Sawano, A. et al., Mol. Cell 68 (3): 626-640.e5 (2017).
| Cat. no. | Plasmid Name | Description |
|---|---|---|
| RDB15273 | mCherry-hCdt1(30/120) / pCSII-EF | EF-1α promoter, hCtd1, mCherry |
| RDB15441 | mCherry-hCdt1(1/100)Cy(-)/pCSII-EF | EF-1α promoter, hCtd1, mCherry |
| RDB15442 | mCherry-hCdt1(1/100)/pCSII-EF | EF-1α promoter, hCtd1, mCherry |
| RDB15271 | mVenus-hGeminin(1/110) / pCSII-EF | EF-1α promoter, hGeminin, mVenus |
| RDB15446 | tFucci(CA)2/pCSII-EF | EF-1α promoter, hCtd1, mCherry, hGeminin, mVenus |
| RDB15452 | tFucci(CA)2/pCSII-CMV | CMV promoter, hCtd1, mCherry, hGeminin, mVenus |
| RDB15447 | tFucci(CA)2.1/pCSII-EF | EF-1α promoter, hCtd1, mCherry, hGeminin, AmCyan |
| RDB15453 | tFucci(CA)2.1/pCSII-CMV | CMV promoter, hCtd1, mCherry, hGeminin, AmCyan |
| RDB15448 | tFucci(CA)2.2/pCSII-EF | EF-1α promoter, hCtd1, mCherry, hGeminin, mTurquoise |
| RDB15454 | tFucci(CA)2.2/pCSII-CMV | CMV promoter, hCtd1, mCherry, hGeminin, mTurquoise |
| RDB15443 | tFucci(SA)2/pCSII-EF | EF-1α promoter, hCtd1, mCherry, hGeminin, mVenus |
| RDB15449 | tFucci(SA)2/pCSII-CMV | CMV promoter, hCtd1, mCherry, hGeminin, mVenus |
| RDB15444 | tFucci(SA)2.1/pCSII-EF | EF-1α promoter, hCtd1, mCherry, hGeminin, AmCyan |
| RDB15450 | tFucci(SA)2.1/pCSII-CMV | CMV promoter, hCtd1, mCherry, hGeminin, AmCyan |
| RDB15445 | tFucci(SA)2.2/pCSII-EF | EF-1α promoter, hCtd1, mCherry, hGeminin, mTurquoise |
| RDB15451 | tFucci(SA)2.2/pCSII-CMV | CMV promoter, hCtd1, mCherry, hGeminin, mTurquoise |
Cell cycle indicator Fucci2
Sakaue-Sawano, A. et al., Chem. Biol. 15 (12): 1243-1248 (2008).
Sakaue-Sawano, A. et al., BMC Cell Biol. 12: 2 (2011).
| Cat. no. | Plasmid Name | Description |
|---|---|---|
| RDB15273 | mCherry-hCdt1(30/120) / pCSII-EF | EF-1α promoter, hCtd1, mCherry |
| RDB15271 | mVenus-hGeminin(1/110) / pCSII-EF | EF-1α promoter, hGeminin, mVenus |
| RDB15274 | AmCyan-hGem(1/110) / pCSII-EF | EF-1α promoter, hGeminin, AmCyan |
Cell cycle indicator Fucci
Sakaue-Sawano, A., Cell 132 (3): 487-498 (2008).
| Cat. no. | Plasmid Name | Description |
|---|---|---|
| RDB15267 | mKO2-hCdt1(30/120) / pCSII-EF | EF-1α promoter, hCtd1, mKO2 |
| RDB15268 | mAG-hGeminin(1/110) / pCSII-EF | EF-1α promoter, hGeminin, mAG |
Cell cycle indicator
Sakaue-Sawano, A. et al., Chem. Biol. 15 (12): 1243-1248 (2008).
| Cat. no. | Plasmid Name | Description |
|---|---|---|
| RDB15269 | mCherry-hGeminin(1/110) / pCSII-EF | EF-1α promoter, hGeminin, mCherry |
| RDB15270 | mCherry-hGeminin(1/60) / pCSII-EF | EF-1α promoter, hGeminin, mCherry |
| RDB15272 | mVenus-hGeminin(1/60) / pCSII-EF | EF-1α promoter, hGeminin, mVenus |
| RDB15274 | AmCyan-hGem(1/110)/pCSII-EF | EF-1α promoter, hGeminin, AmCyan |
Packaging Plasmids
Please visit Lentivirus Vector.
Other Fucci related materials
RIKEN Center for Brain Science (CBS), Dr. Miyawaki lab.
References
Important notice regarding nomenclature of Fucci
- Please read this statement by Dr. Miyawaki [link]
Original papers
- Aiba, Y, et al. (2010) Preferential localization of IgG memory B cells adjacent to contracted germinal centers. Proc. Natl. Acad. Sci. USA. 107 (27): 12192-12197. PMID: 20547847.
- Ando, R., et al. (2020) Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv doi: https://doi.org/10.1101/2020.03.30.015156.
- Ford, M.J. et al. (2018) A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice. Dev. Cell 47 (4): 509-523.e5. PMID: 30458140.
- Mort, R.L. et al. (2014) Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice. Cell Cycle 13 (17): 2681-2696. PMID: 25486356.
- Sakaue-Sawano, A. et al. (2008a) Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498. PMID: 18267078.
- Sakaue-Sawano, A. et al. (2008b) Tracing the silhouette of individual cells in S/G2/M phases with fluorescence. Chem. Biol. 15 (12): 1243-1248. PMID: 19101468.
- Sakaue-Sawano, A. et al. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2. PMID: 21226962.
- Sakaue-Sawano, A. et al. (2013) Visualizing developmentally programmed endoreplication in mammals using ubiquitin oscillators. Development 140 (22): 4624-4632. PMID: 24154524.
- Sakaue-Sawano, A. et al. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5. PMID: 29107535.
- Sugiyama, M. et al. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. USA. 106 (49): 20812-20817. PMID: 19923430.
Mouse of the Month
(GRP0044e 2017.09.18 T.M.)
2022.12.23
