Clone Search

Resources for

Clones & Vectors for Gene Expression
  in E. coli
  in T. thermophilus HB8
  in S. pombe
  in S. cerevisiae
  in Mammalian Cells

Research Tools
  Genome Editing
  Fluorescent Proteins
  Luminescent Proteins
  Sensor & Visualization
  Plant gene resources

Clone Set, Library & Genomic DNA
  Genomic clone
  cDNA clone
  Expression clone
  Libraries
  Genomic DNA

Recombinant Virus
  Recombinant Adenovirus
  Shuttle Vectors

Gene Set Collection
  Autophagy
  Circadian Clock
  Notch Signaling
  Sphingolipid Signaling

Search and Browse
  Key word search
  Browsing by category
  Browsing by species
  Depositors List

Clontech Laboratories, Inc. and RIKEN BioResource Research Center concluded license agreement on preservation and distribution of Fluorescent Proteins, DsRed2 and mCherry for academic use

BRC News

Notice regarding human JUN cDNA pAxCALNLhc-jun(forward) cosmid clone


January 23, 2015

Notice regarding human JUN cDNA pAxCALNLhc-jun(forward) cosmid clone

We, the RIKEN BioResource Research Center, regret to inform you that human JUN cDNA pAxCALNLhc-jun (forward) cosmid clone contains a defect.

The clone was constructed from the human JUN cDNA plasmid clone which was provided by a Japanese researcher. A fragment containing human JUN cDNA was isolated after restriction enzyme digestion and ligated into a cosmid vector. The presence of human JUN was confirmed by terminal end nucleotide sequences (about 500 bp of both ends) of the cosmid clone. The cosmid clone has been for distribution since 2005.

Recently, there was a request for this clone. The sequence analysis of the terminal ends revealed unfortunately that pAxCALNLhc-jun(forward) cosmid clone lacked 567 to 1703 nucleotide, 1137 bp missing (477 bp of 5′ untranslated region and 660 bp of coding region corresponding human JUN gene NM_002228.3 of NCBI database, U.S.A), while it contains 72 to 566 nucleotide and 1704 to 2063 nucleotide. We are afraid that there was a possibility that researchers might not be able to obtain expected results because of this 1137 bp deletion. Therefore, we have decided to delete the pAxCALNLhc-jun (forward) cosmid clone from the web catalog. There is an alternative human JUN cDNA clone in our stock.

We have sent a note to the past users by December 22, 2014, expressing our sincere apology and explaining this matter. In the same note, we also offered an alternative human cDNA to the user.

Recent improvement of sequence analysis of clones makes the great expansion of readable sequences. The advanced technology may reveal that we happened to have unintentionally distributed some resources with defects, such as this clone. We will keep doing our best to improve the quality of our resources to contribute to life science research. We ask you for your understanding and continuous support.

If you have any question and concern on this matter, please feel free to contact us (dnabank.brc@riken.jp ).

(T.M.)