Tetracycline inducible system

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Tetracycline inducible system

Lentivirus vector

Tet-inducible shRNA expression

Cat. no. Plasmid Name Description Data sheet
RDB07916 pENTR4-H1tetOx1 Gateway entry vector, Human H1 promoter, tet operator [Data sheet]
RDB07917 CS-RfA-ETBsd Gateway, EF-1α promoter, TetR-2A-Blasticidin [Data sheet]
RDB08020 CS-RfA-ETV Gateway, EF-1α promoter, TetR-2A-Venus [Data sheet]
RDB08362 CS-RfA-ETR Gateway, EF-1α promoter, TetR-2A-mRFP1 [Data sheet]

Tet-inducible cDNA expression

Cat. no. Plasmid Name Description Data sheet
RDB12876 CSIV-TRE-RfA-CMV-KT Tet-responsive promoter, Gateway, CMV promoter, hKO1-2A-rtTA, loxP [Data sheet]
RDB12878 CSIV-TRE-RfA-UbC-KT Tet-responsive promoter, Gateway, UbC promoter, hKO1-2A-rtTA, loxP [Data sheet]
RDB12872 CSIV-RfA-TRE-EF-KT Tet-responsive promoter, Gateway, EF-1α promoter, hKO1-2A-rtTA, loxP [Data sheet]

Tet-inducible GFP expression

Cat. no. Plasmid Name Description Data sheet
RDB12875 CSIV-TRE-GFP-CMV-KT Tet-responsive promoter, EGFP, CMV promoter, hKO1-2A-rtTA, loxP [Data sheet]
RDB12877 CSIV-TRE-GFP-UbC-KT Tet-responsive promoter, EGFP, UbC promoter, hKO1-2A-rtTA, loxP [Data sheet]
RDB12871 CSIV-GFP-TRE-EF-KT Tet-responsive promoter, EGFP, EF-1α promoter, hKO1-2A-rtTA, loxP [Data sheet]

Plasmid vector

Vector backbone

RDB09232 pBlKS hCMV*-1 b-globinpA
Expression vector of tet-inducible CMV promoter b-globin poly(A) casette.
RDB06012 pAdex TRE-SwaI
Shuttle vector to generate rAd with Tet-responsive element (TRE) promoter

 

Tet regulatory protein expression

RDB18685 pAAV2-SYN-tTAad-BGHpA
AAV2 expression vector of tetracycline-controlled transactivator (tTA Advanced, a.k.a tTAad) driven by the neuron-specific promoter, human synapsin I.
RDB15056 pK039.CAG-loxP-stop-loxP-AmCyan-ires-tTA-WPRE (Supernova)
Cre-based Supernova series. Cre-mediated recombination, a loxP-stop-loxP is excised and the downstream AmCyan is expressed.
RDB15055 pK224.CAG-Rox-STOP-Rox-GFP-ires-tTA-WPRE (Supernova)
Dre-based Supernova series. Expression vector of Rox-stop-Rox-GFP under the CAG promoter.
RDB15047 pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)
Flippase (Flpe)-based Supernova series. Expression vector of FRT-stop-FRT-EGFP under the CAG promoter.
RDB15045 pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)
Cre-based Supernova series. Expression vector of loxP-stop-loxP-EGFP under the CAG promoter.
RDB13212 pAAV-SP-FtTA
AAV vector of FLAG-tTA under the control of Substance P promoter
RDB13211 pAAV-Enk-FtTA
AAV vector of FLAG-tTA under the control of Enkephalin promoter
RDB12109 pBRPyCAG-tTAFF-IN
RDB02351 Ax1 CM-NtTA
Recombinant adenovirus expressing Tet-transactivator with nuclear localization signal
RDB01733 Ax1 CM-NtTA
Recombinant adenovirus expressing Tet-transactivator with nuclear localization signal
RDB01732 Ax1-CA-NtTA
Recombinant adenovirus expressing Tet-transactivator with nuclear localization signal
RDB01731 Ax1-Tet NtTA
Recombinant adenovirus expressing Tet-transactivator with nuclear localization signal
RDB01730 Ax1-RxNtTA
Recombinant adenovirus expressing Tet-transactivator with nuclear localization signal
RDB01711 pAx1-CM-NtTA
Shuttle vector to generate rAd expressing NtTA
RDB01710 pAx1-CA-NtTA
Shuttle vector to generate rAd expressing NtTA
RDB01709 pAx1-Tet NtTA
Shuttle vector to generate rAd expressing NtTA
RDB01708 pAx1-RxNtTA
Recombinant adenoviral vector expressing NtTA (Tet-transactivator)
RDB01684 pCM-NtTA
CM-NtTA (Tet-transactivator driven by CM promoter)
RDB01683 pTet NtTA
Tet-NtTA (Tet-transactivator driven by tet promoter)
RDB01583 pAxCMtTA
Shuttle vector to generate rAd expressing E. coli Tet-inducible tTA gene

 

Positive control vector

RDB01584 pAxTetZ
Shuttle vector to generate rAd expressing tet-inducible lacZ
RDB01574 AxTetZ
Recombinant adenovirus expressing Tet-inducible lacZ gene

2025.05.03 (T.M.)



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