S. pombe ORFeome Clones

Schizosaccharomyces pombe ORFeome clones were deposited by Dr. Minoru Yoshida, Chemical Genetics Lagoratory, RIKEN; These consists of 4,900 entry clones. Clones are provided individually.

Establishing an entire protein assay system of “fission yeast”: Trends from genetic information to entire products in genomic analysis [2006.06.26 RIKEN Press release (pdf)]
Localization data is available at
S. pombe Postgenome Database

  • The Schizosaccharomyces pombe Postgenome Database (SPD) contains the results of systematic analysis of protein expression and localization based on fission yeast ORFeome cloning as well as basic information on genes and proteins.
  • The ORF collection currently covers 99.2% of the fission yeast protein-coding genes and pseudogenes that are reported by the Wellcome Trust Sanger Institute.

Distribution of clones

in Japanese

1. . Distribution of clones

1.1 Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the USER must cite the following literature(s). (Matsuyama et al., 2006, Nature Biotech., 24, 841-847.).
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the USER must state an acknowledgement to the DEPOSITOR.
When the USER wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the USER must obtain a prior written permission from the DEPOSITOR .
When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the USER’s name and his/her affiliation.

1.2 How to obtain clones

Clones are shipped as DNA solution in TE buffer in an individual tube.

Order Forms

Please specify ID of individual clones.

There are three kinds of clones as follows.
Entry clone (SpENT), Cat#. RDB 5820
FLAG2-His6-tagged ORF (SpFFH), Cat#. RDB 6106
YFP-FLAG-His6-tagged ORF (SpYFH), Cat#. RDB 6107
Please reffer Data Sheet for datail.

Please write clone ID in FormA, for example,
01/A01 SPBC1711.15c (SpENT)
01/A01 SPBC1711.15c (SpYFH)
01/B11 SPAC16A10.05c (SpYFH)
01/E01 SPCC191.04c (SpFFH)
  • Order Form (FormA, download site)
    Please complete the form with your shipping information including your account number of an international courier, for example FedEx, World Courier, TNT Express, DHL Global Forwarding and others.
  • Material Transfer Agreement for S. pombe ORFeome Clones FormC6107np, PDF
    We would like to ask a signature of the Authorized Representative of a recipient institution.
    In the the Section 2(a), please write your purpose of use of clone in 10 to 20 words.
  • SCHEDULE A, if your clone contains GFP, CFP and YFP. (GFP bioresources)

Please visit Ordering Information for further information of distribution and fees.

Send forms by regular mail or scanned PDF by e-mail:

The DNA Bank, RIKEN BioResource Research Center,
3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan
E-mail: dnabank.brc@riken.jp
Fax: (+81)-29-836-9120
Distribution fee per clone:
8,440 YEN (For use in research for not-for-profit academic purpose).
(Shipping cost is not included.)

For details for fee and payment, please visit Information of Request for Distribution.
The distribution fee was revised from October 1, 2019

DATA SHEET

data sheet

1. Schizosaccharomyces pombe ORFeome clones
1.2 Clone information
Entry clones
Vector pDONR201 (Kanr), if not specified individually
Insert PCR amplification of GENOMIC DNA of the wild-type strain JY3 Amplified with 5′-G GGG ACA AGT TTG TAC AAA AAA GCA GGC TCT CAT ATG-(template specific sequence)-3′ and 5′-GGG GAC CAC TTT GTA CAA GAA AGC TGG GTA-(template specific sequence)-3′ as primers.
Host DH5alpha, E. coli
FLAG2-His6-tagged ORFs
Vector pDUAL-FFH1c (Ampr), if not specified individually
Insert Corresponding gene was cloned from the entry clone to pDUAL-FFH1c (RDB06142) using the Gateway technology.
Host DH5alpha, E. coli
YFH-tagged ORFs
Vector pDUAL-YFH1c (Ampr), if not specified individually
Insert Corresponding gene was cloned from the entry clone to pDUAL-YFH1c (RDB06151) using the Gateway technology.
Host DH5alpha, E. coli
1.2 Reference
ORFeome_Localizome_data.csv (1.2 MB; 2007.07.02), provided by the depositor of S. pombe ORFeome Clone.

Reference

  • Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Horaoka Y., Horinouchi S., Yoshida M. (2006) ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. Nature Biotech., 24, 841-847.(link)
  • A dataset including the cloning status of the ORFeome entry clones, protein expression levels, and localizome data is available. Please obtain “Supplementary Table 6” of Nat. Biotechnol. 24 (7): 841-847 (2006).
  • Bauer F, Matsuyama A, Candiracci J, Dieu M, Scheliga J, Wolf DA, Yoshida M, Hermand D. (2012) Translational control of cell division by elongator. Cell Rep. 1 (5): 424-433.
  • Bauer F, Matsuyama A, Yoshida M, Hermand D. (2012) Determining proteome-wide expression levels using reverse protein arrays in fission yeast. Nat. Protoc. 7 (10): 1830-1835.
  • Users’ publication Informaion site
    Entry clone
    FLAG2-His6-tagged ORF
    YFP-FLAG-His6-tagged ORF .

Related information

pDUAL vector series
pDUAL2 vector series
pREP vector series
Please find vectors.
Clone(s) is shipped as DNA solution in TE buffer in an individual tube.
Please specify ID of individual clone(s).
 
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(GRP0054e 2006.08.10 T.M.)

2019.08.12



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