Transposon resource
piggyBac®
Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons.
Kim, S.I., Oceguera-Yanez, F., Sakurai, C., Nakagawa, M., Yamanaka, S., Woltjen, K. Methods Mol. Biol. 1357: 111-131 (2016). PubMed PMID 26025620 [PubMed] [Article] [RRC of NBRP]
| Catalog no. | Name of resource | Short Description |
|---|---|---|
| RDB13247 | PB-TAG-ERP2 | All-in-One piggyBac transposon system with constitutive rtTA transactivator and puromycin resistance for dox-inducible expression of Gateway cloned cDNAs and GFP reporter. |
| RDB13246 | PB-TAC-ERP2 | All-in-One piggyBac transposon system with constitutive rtTA transactivator and puromycin resistance for dox-inducible expression of Gateway cloned cDNAs and mCherry reporter. |
| RDB13245 | PB-TA-ERP2 | All-in-One piggyBac transposon system with constitutive rtTA transactivator and puromycin resistance for dox-inducible expression of Gateway cloned cDNAs. |
| RDB13244 | PB-TAG-ERN | All-in-One piggyBac transposon system with constitutive rtTA transactivator and neomycin resistance for dox-inducible expression of Gateway cloned cDNAs and GFP reporter. |
| RDB13243 | PB-TAC-ERN | All-in-One piggyBac transposon system with constitutive rtTA transactivator and neomycin resistance for dox-inducible expression of Gateway cloned cDNAs and mCherry reporter. |
| RDB13242 | PB-TA-ERN | All-in-One piggyBac transposon system with constitutive rtTA transactivator and neomycin resistance for dox-inducible expression of Gateway cloned cDNAs. |
KLF4 N-terminal variance modulates induced reprogramming to pluripotency.
Kim, S.I., Oceguera-Yanez, F., Hirohata, R., Linker, S., Okita, K., Yamada, Y., Yamamoto, T., Yamanaka, S., Woltjen, K. Stem Cell Reports. 4 (4): 727-743 (2015). PubMed PMID 25772473 [PubMed] [Article] [RRC of NBRP]
| Catalog no. | Name of resource | Short Description |
|---|---|---|
| RDB13131 | PB-TAC | piggyBac transposon system for dox-inducible gene expression (with mCherry reporter) |
Unpublished Resource
| Catalog no. | Name of resource | Short Description |
|---|---|---|
| RDB12029 | pPB-hCMV*1-dmutCherryIRESEgfpd1-pA | piggyBac expression vector of dmut::mCherry and EGFP::PEST. TRE and a min-CMV promoter. |
| RDB12022 | pPB-hCMV*1-Egfpd1-pA | piggyBac expression vector of dmut::mCherry and EGFP::PEST. TRE and a min-CMV promoter. |
| RDB12021 | pPB-hCMV*1-dmutCherryP2AEgfpd1-pA | piggyBac expression vector of mutated dmut::mCherry::P2A::EGFP::PEST. TRE and a min-CMV promoter. |
| RDB11977 | pPB-CAG-pac-pA | piggyBac expression vector backbone. CAG promoter, HA-tag and puromycin resistance gene. |
| RDB11975 | pPB-CAG-CreGR-IH | piggyBac expression vector of Cre recombinase with GR ligand binding domain. CAG promoter and hygromycin resistance gene. |
| RDB11913 | pPB-PGKneopA(p) | piggyBac expression vector backbone. PGK promoter and neomycin resistance gene. |
| RDB11902 | pPBCAG-mCherryd2-IP | piggyBac expression vector of mCherry::PEST::PEST. CAG promoter and puromycin resistance gene. |
| RDB11901 | pPBCAG-dCherryd2-IP | piggyBac expression vector of PEST::mCherry::PEST::PEST. CAG promoter and puromycin resistance gene. |
| RDB11900 | pPBCAG-mCherryd1-IP | piggyBac expression vector of mCherry with PEST. CAG promoter and puromycin resistance gene. |
| RDB11899 | pPBCAG-dCherryd1-IP | piggyBac expression vector of PEST::mCherry::PEST. CAG promoter and puromycin resistance gene. |
| RDB11847 | pPBlox-PGKpacpA | piggyBac expression vector backbone. PGK promoter and puromycin resistance gene sandwiched between loxP. |
| RDB11659 | pPBCAG-dEGFP-IP | piggyBac expression vector of EGFP::PEST. CAG promoter and puromycin resistance gene. |
| RDB11630 | pPBCAGcHApA | piggyBac expression vector backbone. CAG promoter and HA-tag. |
| RDB11629 | pPBhCMV*1cHApA | piggyBac expression vector backbone. TRE, min-CMV promoter and HA-tag. |
| RDB11616 | pPB-Ins2-CAG-Egfp-IP | Expression plasmid of EGFP in piggyBac vector. CAG promoter and puromycin resistance gene and flanked by the Evx2::Hoxd13 intergenic regions. |
| RDB11615 | pPB-Ins2-CMVp-mCherry-IP | piggyBac expression vector contains mCherry and puromycin resistance gene via IRES driven by a minimal CMV promoter, inserted between the Evx2::Hoxd13 Intergenic regions. |
| RDB11612 | pPB-CMVp-mCherry-IP | piggyBac expression vector of mCherry. minimal CMV promoter and puromycin resistance gene. |
| RDB11600 | pPB-InsMCSIns(1) | piggyBac vector containing Evx2-Hoxd13 Intergenic Region. |
| RDB11571 | pPB-hCMV*1-mCherry-IH(1) | piggyBac expression vector of mCherry. TRE, min-CMV promoter, hygromycin resistance gene. |
| RDB11569 | pPB-CMVp-mCherry-IP(1) | piggyBac expression vector of mCherry. Minimal CMV promoter and puromycin resistance gene. |
| RDB11568 | pPB-tkp-mCherry-IP | piggyBac expression vector of mCherry. Minimal HSV tk promoter and puromycin resistance gene. |
| RDB11526 | pPB-PGKhphpA(1) | piggyBac expression vector backbone. PGK promoter and hygromycin resistance gene. |
| RDB11517 | pPBCAG-EGFP-IZ | piggyBac expression vector of EGFP. CAG promoter and Zeocin resistance gene. |
Sleeping beauty
Spontaneous development of intratumoral heterogeneity in a transposon-induced mouse model of glioma.
Sumiyoshi, K., Koso, H., Watanabe, S. Cancer Sci. 109 (5): 1513-1523 (2018). PubMed PMID 29575648 [PubMed] [Article] [RRC of NBRP]
| Catalog no. | Name of resource | Short Description |
|---|---|---|
| RDB16060 | pMXs-SB11-IRES-puro | Retroviral expression vector of fish Sleeping Beauty 11 (SB11) transposase in mammalian cells. |
2025.07.08 (T.M. GRP0079e)
