Transposon resource
piggyBac®
SLFN11-mediated ribosome biogenesis impairment induces TP53-independent apoptosis.
Ogawa, A., Izumikawa, K., Tate, S., Isoyama, S., Mori, M., Fujiwara, K., Watanabe, S., Ohga, T., Jo, U., Taniyama, D., Kitajima, S., Tanaka, S., Onji, H., Kageyama, S.I., Yamamoto, G., Saito, H., Morita, T.Y., Okada, M., Natsumeda, M., Nagahama, M., Kobayashi, J., Ohashi, A., Sasanuma, H., Higashiyama, S., Dan, S., Pommier, Y., Murai, J. Mol Cell 85 (5): 894-912.e10 (2025). PubMed PMID 39909041. [PubMed] [Article] [RRC of NBRP]
| Catalog no. | Name of resource | Short Description |
|---|---|---|
| RDB20967 | pPCIH-vector | PiggyBac control vector, including hygromycin resistance gene. |
The crucial role of single-stranded DNA binding in enhancing sensitivity to DNA-damaging agents for Schlafen 11 and Schlafen 13.
Fujiwara, K., Maekawa, M., Iimori, Y., Ogawa, A., Urano, T., Kono, N., Takeda, H., Higashiyama, S., Arita, M., Murai, J. iScience 26 (12): 108529 (2023). PubMed PMID 38125019. [PubMed] [Article] [RRC of NBRP]
| Catalog no. | Name of resource | Short Description |
|---|---|---|
| RDB20961 | pPCIP-vector | PiggyBac control vector, including puromycin resistance gene. |
Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons.
Kim, S.I., Oceguera-Yanez, F., Sakurai, C., Nakagawa, M., Yamanaka, S., Woltjen, K. Methods Mol. Biol. 1357: 111-131 (2016). PubMed PMID 26025620 [PubMed] [Article] [RRC of NBRP]
| Catalog no. | Name of resource | Short Description |
|---|---|---|
| RDB13247 | PB-TAG-ERP2 | All-in-One piggyBac transposon system with constitutive rtTA transactivator and puromycin resistance for dox-inducible expression of Gateway cloned cDNAs and GFP reporter. |
| RDB13246 | PB-TAC-ERP2 | All-in-One piggyBac transposon system with constitutive rtTA transactivator and puromycin resistance for dox-inducible expression of Gateway cloned cDNAs and mCherry reporter. |
| RDB13245 | PB-TA-ERP2 | All-in-One piggyBac transposon system with constitutive rtTA transactivator and puromycin resistance for dox-inducible expression of Gateway cloned cDNAs. |
| RDB13244 | PB-TAG-ERN | All-in-One piggyBac transposon system with constitutive rtTA transactivator and neomycin resistance for dox-inducible expression of Gateway cloned cDNAs and GFP reporter. |
| RDB13243 | PB-TAC-ERN | All-in-One piggyBac transposon system with constitutive rtTA transactivator and neomycin resistance for dox-inducible expression of Gateway cloned cDNAs and mCherry reporter. |
| RDB13242 | PB-TA-ERN | All-in-One piggyBac transposon system with constitutive rtTA transactivator and neomycin resistance for dox-inducible expression of Gateway cloned cDNAs. |
KLF4 N-terminal variance modulates induced reprogramming to pluripotency.
Kim, S.I., Oceguera-Yanez, F., Hirohata, R., Linker, S., Okita, K., Yamada, Y., Yamamoto, T., Yamanaka, S., Woltjen, K. Stem Cell Reports. 4 (4): 727-743 (2015). PubMed PMID 25772473 [PubMed] [Article] [RRC of NBRP]
| Catalog no. | Name of resource | Short Description |
|---|---|---|
| RDB13131 | PB-TAC | piggyBac transposon system for dox-inducible gene expression (with mCherry reporter) |
Unpublished Resource
| Catalog no. | Name of resource | Short Description |
|---|---|---|
| RDB12029 | pPB-hCMV*1-dmutCherryIRESEgfpd1-pA | piggyBac expression vector of dmut::mCherry and EGFP::PEST. TRE and a min-CMV promoter. |
| RDB12022 | pPB-hCMV*1-Egfpd1-pA | piggyBac expression vector of dmut::mCherry and EGFP::PEST. TRE and a min-CMV promoter. |
| RDB12021 | pPB-hCMV*1-dmutCherryP2AEgfpd1-pA | piggyBac expression vector of mutated dmut::mCherry::P2A::EGFP::PEST. TRE and a min-CMV promoter. |
| RDB11977 | pPB-CAG-pac-pA | piggyBac expression vector backbone. CAG promoter, HA-tag and puromycin resistance gene. |
| RDB11975 | pPB-CAG-CreGR-IH | piggyBac expression vector of Cre recombinase with GR ligand binding domain. CAG promoter and hygromycin resistance gene. |
| RDB11913 | pPB-PGKneopA(p) | piggyBac expression vector backbone. PGK promoter and neomycin resistance gene. |
| RDB11902 | pPBCAG-mCherryd2-IP | piggyBac expression vector of mCherry::PEST::PEST. CAG promoter and puromycin resistance gene. |
| RDB11901 | pPBCAG-dCherryd2-IP | piggyBac expression vector of PEST::mCherry::PEST::PEST. CAG promoter and puromycin resistance gene. |
| RDB11900 | pPBCAG-mCherryd1-IP | piggyBac expression vector of mCherry with PEST. CAG promoter and puromycin resistance gene. |
| RDB11899 | pPBCAG-dCherryd1-IP | piggyBac expression vector of PEST::mCherry::PEST. CAG promoter and puromycin resistance gene. |
| RDB11847 | pPBlox-PGKpacpA | piggyBac expression vector backbone. PGK promoter and puromycin resistance gene sandwiched between loxP. |
| RDB11659 | pPBCAG-dEGFP-IP | piggyBac expression vector of EGFP::PEST. CAG promoter and puromycin resistance gene. |
| RDB11630 | pPBCAGcHApA | piggyBac expression vector backbone. CAG promoter and HA-tag. |
| RDB11629 | pPBhCMV*1cHApA | piggyBac expression vector backbone. TRE, min-CMV promoter and HA-tag. |
| RDB11616 | pPB-Ins2-CAG-Egfp-IP | Expression plasmid of EGFP in piggyBac vector. CAG promoter and puromycin resistance gene and flanked by the Evx2::Hoxd13 intergenic regions. |
| RDB11615 | pPB-Ins2-CMVp-mCherry-IP | piggyBac expression vector contains mCherry and puromycin resistance gene via IRES driven by a minimal CMV promoter, inserted between the Evx2::Hoxd13 Intergenic regions. |
| RDB11612 | pPB-CMVp-mCherry-IP | piggyBac expression vector of mCherry. minimal CMV promoter and puromycin resistance gene. |
| RDB11600 | pPB-InsMCSIns(1) | piggyBac vector containing Evx2-Hoxd13 Intergenic Region. |
| RDB11571 | pPB-hCMV*1-mCherry-IH(1) | piggyBac expression vector of mCherry. TRE, min-CMV promoter, hygromycin resistance gene. |
| RDB11569 | pPB-CMVp-mCherry-IP(1) | piggyBac expression vector of mCherry. Minimal CMV promoter and puromycin resistance gene. |
| RDB11568 | pPB-tkp-mCherry-IP | piggyBac expression vector of mCherry. Minimal HSV tk promoter and puromycin resistance gene. |
| RDB11526 | pPB-PGKhphpA(1) | piggyBac expression vector backbone. PGK promoter and hygromycin resistance gene. |
| RDB11517 | pPBCAG-EGFP-IZ | piggyBac expression vector of EGFP. CAG promoter and Zeocin resistance gene. |
Sleeping beauty
Spontaneous development of intratumoral heterogeneity in a transposon-induced mouse model of glioma.
Sumiyoshi, K., Koso, H., Watanabe, S. Cancer Sci. 109 (5): 1513-1523 (2018). PubMed PMID 29575648 [PubMed] [Article] [RRC of NBRP]
| Catalog no. | Name of resource | Short Description |
|---|---|---|
| RDB16060 | pMXs-SB11-IRES-puro | Retroviral expression vector of fish Sleeping Beauty 11 (SB11) transposase in mammalian cells. |
2025.09.01 (T.M. GRP0079e)
