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Nakamura and White RFLP clones


Nakamura & White RFLP clone List

RDB No. HPR01001 Name of clone pMCR3
Chromosome 1p Locus
Vector pUC18 Cloning site EcoR I Insert size 6.0 kb
Comment N – Ras Tight linkage to NGFb Linkage analysis FY NGFb PGMI
Reference Human Genome Mapping 9
Nucleic Acids Research, 15, 9623 (1987)
Developer M. Carlson, Y. Nakamura and R. White Depositor Y. Nakamura
Description A 6.0 kb EcoR I fragment from the cosmid cMCR3 (isolated by N-Ras cDNA probe) was subcloned into EcoR I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP EcoR I (E1 : 6.0 kb, E2 : 4.8 kb). Not polymorphic for Msp I, Taq I, Rsa I, Bgl II, Pst I, Pvu II, BamH I, Hind III.
RDB No. HPR01002 Name of clone pTHI54
Chromosome 1p Locus D1S62
Vector pBR322 Cloning site Hind III Insert size 13.0 kb
Comment Insulin Linkage analysis FY NRAS NGFb RH
Reference Nucleic Acids Research, 16, 3115 (1988)
Human Genome Mapping 9
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 13.0 kb Hind III fragment from the phage THI54 (isolated by the oligonucleotides of insulin coding region) was subcloned into the Hind III site of pBR322.
Host HB101 Genetic Marker Amp
RFLP Pvu II, BamH I, Bgl II, EcoR I, Hind III, Msp I, Pst I, Rsa I, Taq I (P1 : 6.0 kb, P2 : 5.0 kb).
RDB No. HPR01004 Name of clone pEFZ13
Chromosome 1p Locus D1S64
Vector pUC18 Cloning site Taq I / Acc I Insert size 5.5 kb
Comment Linkage analysis FY NRAS NGFb (multipoint linkage analysis)
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 5704 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 5.5 kb Taq I fragment from the cosmid cEFZ13 was subcloned into the Acc I site of pUC18.
Host Genetic Marker Amp
RFLP Msp I (M1 : 5.0 kb, M2 : 3.3 kb). Constant bands (1.6 kb, 0.7 kb, 0.6 kb). Not polymorphic for Bgl II, Pst I, Pvu II, Rsa I, Taq I.
RDB No. HPR01006 Name of clone pYNZ2
Chromosome 1p Locus D1S57
Vector pBR322 Cloning site Hind III Insert size 3.0 kb
Comment VNTR Linkage analysis (multipoint linkage analysis)
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 4747 (1988)
Developer Depositor Y. Nakamura and R. White
Description A 3.0 kb Hind III fragment from the cosmid cYNZ2 (isolated by the oligonucleotides of zetaglobin coding region) was subcloned into the Hind III site of pBR322.
Host HB101 Genetic Marker Amp
RFLP Msp I, Rsa I, Taq I, Bgl II, Pst I, Pvu II, EcoR I, BamH I, Hind III (VNTR polymorphism with bands between 1.0 kb and 3.0 kb).
RDB No. HPR01007 Name of clone pYNZ23
Chromosome 1q Locus D1S58
Vector pBR322 Cloning site Msp I / Cla I Insert size 5.0 kb
Comment Linkage analysis REN FY (multipoint linkage analysis)
Reference Human Genome Mapping 9
Nucleic Acids Research, 15, 9620 (1987)
Developer Depositor Y. Nakamura and R. White
Description A 5.0 kb Msp I fragment from the cosmid cYNZ23 (isolated by the oligonucleotides of zetaglobin coding regin) was subcloned into the Cla I site of pBR322.
Host HB101 Genetic Marker Amp
RFLP Msp I, BamH I, Bgl II, EcoR I, Hind III, Pst I, Pvu II, Rsa I, Taq I (M1 : 5.0 kb, M2 : 4.5 kb).
RDB No. HPR01008 Name of clone pMLAJ1
Chromosome 1 Locus D1S61
Vector pBR322 Cloning site EcoR I – Hind III Insert size 0.8 kb
Comment VNTR Myoglobin Linkage analysis REN FY NGFb
Reference Human Genome Mapping 9
Nucleic Acids Research, 19, 4801 (1991)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 0.8 kb Hind III – EcoR I fragment (isolated from cMLAJ1 using the oligonucleotides of myoglobin coding region) was subcloned into Hind III-EcoR I site of pBR322.
Host HB101 Genetic Marker Amp
RFLP Hinf I, Bgl II, EcoR I, Hind III, Msp I, Pst I, Pvu II, Rsa I, Taq I (VNTR polymorphism with alleles between 1.5 kb and 3.0 kb).
RDB No. HPR01010 Name of clone pHBI40
Chromosome 1q Locus D1S66
Vector pUC18 Cloning site Msp I / Acc I Insert size 4.4 kb
Comment Linkage analysis REN FY NRAS
Reference Human Genome Mapping 9
Nucleic Acids Research, 15, 9622 (1987)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 4.4 kb Msp I fragment from the cosmid cHBI40 was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I (M1 : 8.0 kb, M2 : 4.4 kb). Not polymorphic for Taq I, Rsa I.
RDB No. HPR01011 Name of clone pHHH106
Chromosome 1 Locus D1S67
Vector pUC18 Cloning site Taq I / Acc I Insert size 2.2 kb
Comment Linkage analysis FY NRAS NGFb
Reference Human Genome Mapping 9
Nucleic Acids Research , 16, 5218 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 2.2 kb Taq I fragment from the cosmid cHHH106 was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I (M1 : 2.3 kb, M2 : 2.0 kb). Not polymorphic for Rsa I, Taq I.
RDB No. HPR01012 Name of clone pEFD53.2
Chromosome 1 Locus D1S73
Vector pUC18 Cloning site Taq I / Acc I Insert size 4.7 kb
Comment Linkage analysis FY NRAS NGFb
Reference Nucleic Acids Research, 16, 3116 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 4.7 kb Taq I fragment from the cosmid cEFD53 was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Taq I (T1 : 7.0 kb, T2 : 4.7 kb). Msp I (M1 : 2.3 kb, M2 : 1.9 kb). Not polymorphic for Bgl II, Pst I, Pvu II and Rsa I.
RDB No. HPR01013 Name of clone pTHH33
Chromosome 1 Locus D1S81
Vector pUC18 Cloning site EcoR I Insert size 5.6 kb
Comment VNTR Linkage analysis
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 9363 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 5.6 kb EcoR I fragment from the cosmid cTHH33 (isolated with the oligonucleotides of HBV-2 coding region) was subcloned into the EcoR I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Rsa I, Taq I, Bgl II, Pst I, Pvu II, BamH I, EcoR I, Msp I, Hind III (VNTR polymorphism with bands between 4.0 kb and 6.5 kb).
RDB No. HPR01014 Name of clone pMCT118
Chromosome 1p Locus D1S80
Vector pUC18 Cloning site Pst I Insert size 3.1 kb
Comment VNTR Linkage analysis
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 9364 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 3.1 kb Pst I fragment from the cosmid cMCT118 [isolated with the oligonucleotides (GTGTGTGTGTGTGTGTGTGT ) ] was subcloned into the Pst I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Hinf I, Rsa I, Taq I, Msp I, Pvu II, Hae III, Pst I (VNTR polymorphism with bands between 0.3 kb and 1.2 kb).
RDB No. HPR01015 Name of clone pMCT58
Chromosome 1p Locus D1S77
Vector pUC18 Cloning site Pst I Insert size 3.0 kb
Comment VNTR Linkage analysis
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 9367 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 3.0 kb Pst I fragment from the cosmid cMCT58 [isolated by the oligonucleotides (GTGTGTGTGTGTGTGTGTGT) ] was subcloned into the Pst I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Pvu II, Rsa I, Taq I, Bgl II, Pst I (VNTR polymorphism with bands between 1.3 kb and 1.6 kb).
RDB No. HPR01016 Name of clone pCMM12(pCMM12.1)
Chromosome 1 Locus D1S76
Vector pUC18 Cloning site Taq I / Acc I Insert size 5.3 kb
Comment VNTR Linkage analysis (multipoint linkage analysis)
Reference Nucleic Acids Research, 16, 9368 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 5.3 kb Taq I fragment from the cosmid cCMM12 (isolated by the oligonucleotides of myoglobin-2 coding region) was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Taq I, Bgl II, Pst I, BamH I, Hind III (VNTR polymorphism with bands between 3.0 kb and 4.0 kb).
RDB No. HPR02001 Name of clone pYNH24
Chromosome 2 Locus D2S44
Vector pUC18 Cloning site Msp I / Acc I Insert size 2.0 kb
Comment VNTR Linkage analysis APOB D2S6
Reference Human Genome Mapping 9
Nucleic Acids Research, 15, 10073 (1987)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 2.0 kb Msp I fragment from the cosmid cYNH24 [isolated by HBV-2 oligonucleotides (GGAGTTGGGGGAGGAG) ]was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I, Taq I, Bgl II, Pvu II, Pst I, BamH I (VNTR polymorphism with bands between 1.0 kb and 5.0 kb). Not polymorphic for Rsa I.
RDB No. HPR02002 Name of clone pYNZ15
Chromosome 2 Locus D2S50
Vector pBR322 Cloning site BamH I Insert size 3.1 kb
Comment Linkage analysis APOB D2S6
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 5702 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 3.1 kb BamH I fragment from the cosmid cYNZ15 was subcloned into the BamH I site of pBR322.
Host HB101 Genetic Marker Amp
RFLP Taq I (T1 : 1.8 kb, T2 : 0.9 kb). Not polymorphic for BamH I, Bgl II, EcoR I, Hind III, Pst I, Pvu II, Msp I.
RDB No. HPR02003 Name of clone pHHH133
Chromosome 2 Locus D2S45
Vector pUC18 Cloning site EcoR I Insert size 7.0 kb
Comment Linkage analysis APOB D2S6
Reference Human Genome Mapping 9
Nucleic Acids Research, 15, 10075 (1987)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 7.0 kb fragment from the cosmid cHHH133 was subcloned into the EcoR I site of pUC 18.
Host JM109 Genetic Marker Amp
RFLP Msp I (M1 : 1.4 kb, M2 : 1.3 kb) with a constant band at 4.0 kb, 1.0 kb. Not polymorphic for Rsa I, Pst I, Bgl II, Pvu II, Taq I.
RDB No. HPR02004 Name of clone pYNZ9.1
Chromosome 2 Locus D2S46
Vector pUC18 Cloning site Msp I / Acc I Insert size 6.6 kb
Comment Linkage analysis APOB D2S6
Reference Human Genome Mapping 9
Nucleic Acids Research, 15, 10074 (1987)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 6.6 kb Msp I fragment from the cosmid cYNZ9.1 was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Taq I (T1 : 1.1 kb, M2 : 1.0 kb). Not polymorphic for BamH I, Bgl II, EcoR I, Hind III, Pst I, Pvu II, Msp I.
RDB No. HPR02005 Name of clone pTBAB5.7
Chromosome 2 Locus D2S47
Vector pUC18 Cloning site Msp I / Acc I Insert size 3.3 kb
Comment VNTR Linkage analysis ACP D2S1
Reference Human Genome Mapping 9
Nucleic Acids Research, 15, 10072 (1987)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 3.3 kb Msp I fragment from the cosmid cTBA5.7 was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Pvu II, Rsa I, Bgl II (VNTR polymorphism with bands between 4.1 kb and 5.0 kb). Not polymorphic for Taq I, BamH I, Hind III.
RDB No. HPR02006 Name of clone pEFD122
Chromosome 2 Locus D2S48
Vector pUC18 Cloning site Msp I / Acc I Insert size 2.1 kb
Comment Linkage analysis APOB D2S6
Reference Human Genome Mapping 9
Nucleic Acids Research, 15, 10078 (1987)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 2.1 kb Msp I fragment from the cosmid cEFD122 was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I (M1 : 2.3 kb, M2 : 2.1 kb) without a constant band. Not polymorphic for Rsa I, Taq I.
RDB No. HPR02007 Name of clone pYNA15.1
Chromosome 2 Locus D2S49
Vector pUC18 Cloning site EcoR I Insert size 2.2 kb
Comment Linkage analysis ACP D2S1
Reference Human Genome Mapping 9 Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 2.2 kb EcoR I fragment from the cosmid cYNA15 was subcloned into the EcoR I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I (M1 : 4.1 kb, M2 : 3.5 kb). Not polymorphic for Taq I, Rsa I.
RDB No. HPR02009 Name of clone pCMM63
Chromosome 2 Locus D2S51
Vector pUC18 Cloning site Rsa I / Hinc II Insert size 3.9 kb
Comment Myoglobin – 2 Linkage analysis APOB D2S6
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 9361 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 3.9 kb Rsa I fragment from the cosmid cMM63 (isolated by the oligonucleotides of the myoglobin-2 coding region) was subcloned into the Hinc II site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I, Taq I, Rsa I, Pst I (M1: 6.0 kb, M2 : 4.5 kb, M3 : 4.4 kb). Not polymorphic for Pvu II.
RDB No. HPR02010 Name of clone pEKZ105
Chromosome 2 Locus D2S55
Vector pUC18 Cloning site Rsa I / Hinc II Insert size 3.0 kb
Comment Linkage analysis CRYG D2S3
Reference Human Genome Mapping 9
Nucleic Acids Research, 15, 10076 (1987)
Developer Depositor Y. Nakamura and R. White
Description A 3.0 kb Rsa I fragment from the cosmid cEKZ105 was subcloned into the Hinc II site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Rsa I (R1 : 3.0 kb, R2 : 2.6 kb). Not polymorphic for BamH I, Bgl II, EcoR I, Hind III, Pst I, Pvu II, Rsa I, Msp I.
RDB No. HPR02012 Name of clone pHHH115.2
Chromosome 2 Locus D2S54
Vector pUC18 Cloning site Msp I / Acc I Insert size 1.7 kb
Comment Linkage analysis APOB D2S6
Reference Human Genome Mapping 9
Nucleic Acids Research, 15, 10077 (1987)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 1.7 kb Msp I fragment from the cosmid cHHH115 was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I (M1 : 2.4 kb, M2 : 1.7 kb) without a constant band. Not polymorphic for Rsa I, Pst I, Bgl II, Pvu II, Taq I.
RDB No. HPR02013 Name of clone pMCT106
Chromosome 2 Locus D2S61
Vector pUC18 Cloning site Msp I / Acc I Insert size 1.7 kb
Comment VNTR Linkage analysis ACP D2S1
Reference Nucleic Acids Research, 16, 9359 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 1.7 kb Msp I fragment from the cosmid cMCT106 [isolated by the oligonucleotides (GTGTGTGTGTGTGTGTGTGT) ]was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Pvu II, Msp I, Taq I, Rsa I, Pst I (VNTR polymorphism with bands between 2.0 kb and 3.5 kb) without constant band.
RDB No. HPR02014 Name of clone pEFZ38
Chromosome 2 Locus D2S60
Vector pUC18 Cloning site Rsa I / Hinc II Insert size 4.0 kb
Comment Linkage analysis ACP D2S1
Reference Nucleic Acids Research, 16, 9360 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 4.0 kb Rsa I fragment from the cosmid cEFZ38 was subcloned into the Hinc II site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Rsa I (R1 : 4.0 kb, R2 : 3.4 kb) without a constant band. Taq I (2.0 kb, 1.7 kb)—this is not yet examined by three generations of family. Not polymolphic for Msp I.
RDB No. HPR03001 Name of clone pMS1-37
Chromosome 3p Locus (D3S3)
Vector pBR322 Cloning site EcoR I – Hind III Insert size 2.5 kb
Comment Linkage analysis
Reference Human Genome Mapping 9 Hum. Genet. 83, 127-132 (1989) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description
Host HB101 Genetic Marker Amp
RFLP Msp I (3.65 – 1.2 kb, 4.85 kb). Not polymorphic for Mbo I, Taq I, EcoR I, Hind III, Pst I, BamH I, Kpn I, Pvu II, Bgl II, Bcl I, Hinc II, Sst I, Xma I, Hae III, BstN I, BstE II, Acc I, Stu I, Rsa I, EcoR V, Sin I, Hinf I, Xba I, Dde I, Nae I.
RDB No. HPR03002 Name of clone pYNZ86.1
Chromosome 3 Locus D3S30
Vector pUC18 Cloning site Hind III Insert size 3.3 kb
Comment Linkage analysis DNF15S2 D3S4
Reference Human Genome Mapping 9
Nucleic Acids Research, 15, 10079 (1987)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 3.3 kb Hind III fragment from the cosmid cYNZ86 was subcloned into the Hind III site of pUC18.
Host HB101 Genetic Marker Amp
RFLP Msp I (M1 : 2.0 kb, M2 : 1.9 kb) with constant bands at 2.6 and 1.7 kb. Pvu II (P1 : 1.1 kb, p2 : 0.8 kb) with a constant band at 1.5 kb (This observation has not been verified by pedigree studies). Not polymorphic for Taq I, Rsa I, Pst I, Bgl II, EcoR I, BamH I and Hind III.
RDB No. HPR03003 Name of clone pMCT32.1
Chromosome 3 Locus D3S31
Vector pUC18 Cloning site Taq I / Acc I Insert size 2.9 kb
Comment Linkage analysis CP
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 9358 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 2.9 kb Taq I fragment from the cosmid cMCT32 was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Pvu II (P1 : 15.0 kb, P2 : 14.0 kb, P3 : 12.0 kb) with constant band at 1.0 kb. Msp I and Taq I (M1 : 10.0 kb,M2 : 7.0 kb and T1 : 5.5 kb, T2 : 2.9 kb) without a constant band (This observation has not been verified by pedigree studies). Not polymorphic for Rsa I, Pst I, Bgl II, EcoR I, BamH I and Hind III.
RDB No. HPR03004 Name of clone pEFD145.1(pEFD145)
Chromosome 3 Locus D3S32
Vector pUC18 Cloning site Taq I / Acc I Insert size 4.1 kb
Comment Linkage analysis DNF15S2 D3S4
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 9357 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 4.1 kb Taq I fragment from the cosmid cEFD145 was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Rsa I (R1 : 2.4 kb, M2 : 1.4 kb and 1.0 kb) with a constant band at 0.9 kb. Taq I (T1 : 5.0 kb, T2 : 4.1 kb) without a constant band (This observation has not been verified by pedigree studies). Not polymorphic for Msp I.
RDB No. HPR03005 Name of clone pEFD64.1
Chromosome 3 Locus D3S42
Vector pUC18 Cloning site Taq I / Acc I Insert size 5.2 kb
Comment VNTR HBV-3 Linkage analysis APOD
Reference Nucleic Acids Research, 16, 10947 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 5.2 kb Taq I fragment of the cosmid cEFD64 (isolated by the oligonucleotides of HBV-3) was subcloned into Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Hinf I (VNTR polymorphisms with bands between 0.5kb and 2.5 kb [system 1], or 1.7 kb and 1.9 kb [system 2]). Taq I, Rsa I, Bgl II, EcoR I, BamH I, Hind III and Pvu II (system 1 only). Not polymorphic for Bgl II.
RDB No. HPR03006 Name of clone pEFD64.2
Chromosome 3 Locus D3S46
Vector pUC18 Cloning site Taq I / Acc I Insert size 4.0 kb
Comment VNTR HBV-3 Linkage analysis APOD
Reference Nucleic Acids Research, 16, 9354 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 4.0 kb Taq I fragment of the cosmid cEFD 64 (isolated by the oligonucleotides of HBV-3) was subcloned into Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I, Rsa I, Taq I, EcoR I, BamH I, Hind III and Pvu II (VNTR polymorphism with bands between 2.6 kb and 4.6 kb). Not polymorphic for Bgl II.
RDB No. HPR03007 Name of clone pEFD134.7
Chromosome 3 Locus D3S45
Vector pUC18 Cloning site Msp I / Acc I Insert size 4.3 kb
Comment VNTR HBV-3 Linkage analysis APOD
Reference Nucleic Acids Research, 16, 9355 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 4.3 kb Msp I fragment of the cosmid cEFD134 (isolated by the oligonucleotides of HBV-3) was subcloned into Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I, Rsa I and Taq I (VNTR polymorphism with bands between 1.5 kb and 2.7 kb).
RDB No. HPR04001 Name of clone pMCOC14
Chromosome 4p Locus D4S125(D4S10)
Vector pUC18 Cloning site Rsa I / Hinc II Insert size 3.5 kb
Comment VNTR Linkage analysis D4S10
Reference Nucleic Acids Research, 16, 6254 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 3.5 kb Pst I fragment from the cosmid cMCOC14 [isolated by the oligonucleotides (GGGNGTGGGGGNGTNNNG) ] was subcloned into the Pst I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Pst I, Taq I, Rsa I, Msp I (VNTR polymorphism with bands between 2.5 kb and 2.7 kb) without a constant band.
RDB No. HPR06001 Name of clone pHHH157
Chromosome 6p Locus D6S29
Vector pUC18 Cloning site BamH I Insert size 5.5 kb
Comment Linkage analysis GLO D6S8 HLA -D D6S10
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 5217 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 5.5 kb BamH I fragment from the cosmid cHHH157 was subcloned into BamH I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP BamH I (B1 : 13.0 kb, B2 : 5.5 kb). Not polymorphic for Msp I, Rsa I, Taq I, Bgl II, Pst I, Pvu II, EcoR I, Hind III.
RDB No. HPR06003 Name of clone p7H4
Chromosome 6 Locus D6S7
Vector pBR322 Cloning site EcoR I – Hind III Insert size 2.0 kb
Comment Linkage analysis
Reference Human Genome Mapping 9 Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description
Host HB101 Genetic Marker Amp
RFLP EcoR V (11 kb, 10 kb, 9.5 kb, 9.0 kb). BamH I (8 kb, 6 kb). Not polymorphic for Msp I, Taq I, Bal I, Bgl II, Pvu II, EcoR V.
RDB No. HPR06004 Name of clone pHHH171
Chromosome 6p Locus D6S38
Vector pUC18 Cloning site Msp I / Acc I Insert size 2.3 kb
Comment Linkage analysis GLO D6S8 HLA-D D6S10
Reference Nucleic Acids Research, 16, 4742 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 2.3 kb Msp I fragment from the cosmid cHHH171 was subcloned into Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I (M1: 2.3 kb, M2 : 1.9 kb). Not polymorphic for Rsa I, Taq I, Bgl II, Pvu II, EcoR I, Hind III.
RDB No. HPR06005 Name of clone pJCZ30
Chromosome 6 Locus D6S37
Vector pUC18 Cloning site Rsa I / Hinc II Insert size 3.0 kb
Comment VNTR Linkage analysis D6S2
Reference Nucleic Acids Research, 16, 4743 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 3.0 kb Rsa I fragment from the cosmid cJCZ30 (isolated with the oligonucleotides of zetaglobin) was subcloned into the Hinc II site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Hind III, Msp I, Taq I, Rsa I, Pst I, Pvu II, EcoR I, BamH I (VNTR polymorphism with bands between1.0 kb and 3.0 kb).
RDB No. HPR06006 Name of clone pYNZ132
Chromosome 6 Locus D6S40
Vector pUC18 Cloning site Msp I / Acc I Insert size 1.6 kb
Comment VNTR Zetaglobin Linkage analysis D6S2
Reference Nucleic Acids Research, 16, 9889 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 1.6 kb Msp I fragment from the cosmid cYNZ132 (isolated with the oligonucleotides of the zetaglobin) was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Taq I, Msp I, Rsa I, Pst I, Pvu II (VNTR polymorphism with bands between 1.2 kb and 1.8 kb). Not polymorphic for Bgl II, EcoR I.
RDB No. HPR06007 Name of clone pEFD6
Chromosome 6 Locus D6S41
Vector pUC18 Cloning site Pst I Insert size 4.0 kb
Comment Linkage analysis D6S2
Reference Nucleic Acids Research, 16, 9888 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 4.0 kb Pst I fragment from the cosmid cEFD6 (isolated with the oligonucleotides of HBV-3) was subcloned into the Pst I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Pst I, Bgl II, Msp I, Rsa I, Pvu II (VNTR polymorphism with a band 4.0 kb or 3.5 kb).
RDB No. HPR06008 Name of clone pTHH5
Chromosome 6 Locus D6S39
Vector pBR322 Cloning site Pst I Insert size 5.4 kb
Comment VNTR HBV – I Linkage analysis D6S2
Reference Human Genome Mapping 9 Genomics, 16, 426-430 (1993) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 5.4 kb Pst I fragment from the cosmid cTHH5 (isolated with the oligonucleotides of HBV-1) was subcloned into the Pst I site of pBR322.
Host HB101 Genetic Marker Amp
RFLP Hind III, Msp I, Taq I, Rsa I, Pst I, Pvu II, Bgl II, EcoR I, BamH I (VNTR polymorphism with bands between 2.5 kb and 4.0 kb).
RDB No. HPR06009 Name of clone pMCOB12
Chromosome 6 Locus
Vector pUC18 Cloning site Pst I / Hinc II Insert size 3.8 kb
Comment VNTR Linkage analysis D6S2
Reference Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 3.8 kb Pst I fragment from the cosmid cMCOB12 (isolated with the oligonucleotides) was subcloned into the Hinc II site of pUC18.
Host Genetic Marker Amp
RFLP Hind III, Msp I, Taq I, Rsa I, Pst I, Pvu II, EcoR I, BamH I (VNTR polymorphism with bands between 1.0 kb and 3.0 kb).
RDB No. HPR06010 Name of clone p3B10
Chromosome 6 Locus
Vector pBR322 Cloning site Hind III Insert size 2.0 kb
Comment Linkage analysis
Reference Developer Depositor Y. Nakamura and R. White
Description RL
Host Genetic Marker Amp
RFLP Msp I (11 kb, 10 kb, 7 kb).
RDB No. HPR07001 Name of clone pRMU7.4
Chromosome 7p Locus D7S370
Vector pUC18 Cloning site Msp I / Acc I Insert size 2.3 kb
Comment Linkage analysis COL1A2 TCRG
Reference Nucleic Acids Research, 16, 3591 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 2.3 kb Msp I fragment from the cosmid cRMU7 [isolated with probe 742 (P. Pers) ] was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I (M1 : 5.5 kb, M2 : 3.4 kb). Not polymorphic for BamH I, EcoR I, Pst I, Pvu II, Rsa I, Taq I.
RDB No. HPR07002 Name of clone pTHH28
Chromosome 7 Locus D7S371
Vector pBR322 Cloning site Msp I / Cla I Insert size 2.5 kb
Comment Linkage analysis COL1A2 TCRG
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 9887 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 2.5 kb Msp I fragment from the cosmid cTHH28 was subcloned into Cla I site of pBR322.
Host HB101 Genetic Marker Amp
RFLP Msp I (M1 : 3.5 kb, M2 : 2.5 kb).
RDB No. HPR07003 Name of clone pYNB3.1
Chromosome 7q Locus
Vector pUC18 Cloning site Rsa I / Hinc II Insert size 4.0 kb
Comment Linkage analysis COL1A2 MET TCRB
Reference Human Genome Mapping 9 Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description 4.0 kb Rsa I fragment from the cosmid cYNB 3.1 (isolated by pMKA) cloned into Hinc II site for pUC18.
Host JM109 Genetic Marker Amp
RFLP Rsa I (R1 : 3.8 kb, R2 : 2.4 kb, 1.4 kb).
RDB No. HPR07004 Name of clone pCMI37
Chromosome 7 Locus D7S368
Vector pBR322 Cloning site Msp I / Cla I Insert size 3.5 kb
Comment Linkage analysis
Reference Nucleic Acids Research, 16, 4192 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 3.5 kb Msp I fragment from the cosmid cCMI37 identified by the oligonucleotides of the insulin coding region cloned into Cla I site of pBR322.
Host HB101 Genetic Marker Amp
RFLP System A : Rsa I (RA1 : 5.5 kb, RA2 : 4.8 kb, RA3 : 4.6 kb). System B : Rsa I, Msp I, Taq I (VNTR polymorphism from 2.5 kb – 3.0 kb). System C : Rsa I (RC1 : 2.4 kb, RC2 : 3.3 kb).Not polymorphic for EcoR I, Hind III, Pvu II, Bgl II, Pst I, BamH I neither detect the site polymorphism nor adequately resolve the VNTR polymorphism.
RDB No. HPR07005 Name of clone JCZ67.14I2
Chromosome 7 Locus
Vector Cloning site Insert size kb
Comment Linkage analysis
Reference Developer Depositor
Description
Host Genetic Marker
RFLP
RDB No. HPR07006 Name of clone HHM232.14J1
Chromosome 7 Locus
Vector Cloning site Insert size kb
Comment Linkage analysis
Reference Developer Depositor
Description
Host Genetic Marker
RFLP
RDB No. HPR08001 Name of clone pYNM3
Chromosome 8 Locus D8S38
Vector pUC18 Cloning site Hind III Insert size 3.0 kb
Comment VNTR Linkage analysis D8S5
Reference Human Genome Mapping 9
Nucleic Acids Research, 15, 10080 (1987)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 3.0 kb Hind III fragment from the cosmid cYNM 3 (isolated by the oligonucleotides of myoglobin-1 coding region) was subcloned into the Hind III site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I, Pvu II, EcoR I, Taq I, Rsa I, BamH I, Pst I, Hind III (VNTR polymorphism with bands between 3.2 kb and 3.6 kb).
RDB No. HPR08002 Name of clone pMCT128.2
Chromosome 8 Locus D8S39
Vector pUC18 Cloning site Taq I / Acc I Insert size 3.8 kb
Comment VNTR Linkage analysis D8S5
Reference Nucleic Acids Research, 16, 3590 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 3.8 kb Taq I fragment from the cosmid cMCT128 [isolated by the oligonucleotides (GTGTGTGTGTGTGTGTGTGTGT) ]was subcloned into the AccI site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Pst I, Pvu II, Taq I (VNTR polymorphism with bands between 1.0 kb and 1.6 kb).
RDB No. HPR09004 Name of clone pEFD126.3
Chromosome 9q Locus D9S7
Vector pUC18 Cloning site BamH I / Acc I Insert size 4.2 kb
Comment HBV-4 VNTR Linkage analysis ABO ABL AK1(Adenylate kinase 1) ORM
Reference Human Genome Mapping 9
Nucleic Acids Research, 15, 10607 (1987)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 4.2 kb BamH I fragment from EFD126 isolated by the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Taq I, Bgl II, Msp I, Pst I, Pvu II, Rsa I (VNTR polymorphism with alleles between 1.5 kb and 2.0 kb).
RDB No. HPR09005 Name of clone pMHZ10
Chromosome 9q Locus D9S11
Vector pGEM4 Cloning site Pst I Insert size 3.8 kb
Comment VNTR Linkage analysis ABO AK1(Adenylate kinase 1) ABL
Reference Human Genome Mapping 9 NAR, 15, 10609 (1987) Genomics, 3, 361 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 3.8 kb Pst I fragment from the cosmid cMHZ10 isolated with the oligonucleotides of Z23 was subcloned into the Pst I site of pGEM4.
Host Genetic Marker
RFLP Msp I, EcoR I, Hind III, Pst I, Pvu II (VNTR polymorphism with alleles between 1.4 kb and 2.0 kb). Not polymorphic for Rsa I, Taq I.
RDB No. HPR09006 Name of clone pMCOA12
Chromosome 9q Locus D9S28
Vector pUC18 Cloning site Msp I / Acc I Insert size 3.0 kb
Comment Linkage analysis ABBO AK1 (Adenylate kinase 1) ABL
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 4744 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 3.0 kb Msp I fragment from the cosmid cMCOA12 was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I (M1 : 3.0 kb, M2 : 2.5 kb). Taq I also reveals polymorphism, but this observation has not been confirmed by pedigree studies. Not polymorphic for Bgl II, Pst I, Pvu II, Rsa I.
RDB No. HPR09007 Name of clone pEFD40.3
Chromosome 9 Locus D9S8
Vector pUC18 Cloning site Taq I / Acc I Insert size 3.0 kb
Comment Linkage analysis ORM( Orosomucoid ) D9S1 AS1 GALT (Galactose 1-P-uridyltransferase)
Reference Human Genome Mapping 9
Nucleic Acids Research, 15, 10612 (1987)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 3.0 kb Taq I fragment from the cosmid cEFD40 was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I (M1: 5.3 kb, M2 : 4.4 kb). Not polymorphic for Bgl II, Pst I, Pvu II, Rsa I, Taq I.
RDB No. HPR09008 Name of clone pEKZ130
Chromosome 9 Locus D9S9
Vector pUC18 Cloning site Taq I / Acc I Insert size 2.8 kb
Comment Linkage analysis ORM D9S1 AS1 GALT (Galactose 1-P-uridyltransferase)
Reference Human Genome Mapping 9 NAR, 15, 10611 (1987) Genomics, 3, 361 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 2.8 kb Taq I fragment from the cosmid cEKZ130 was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I (M1 : 6.2 kb, M2 : 4.5 kb). Not polymorphic for Bgl II, Pst I, Pvu II, Rsa I, Taq I.
RDB No. HPR09009 Name of clone pTHH22
Chromosome 9q Locus D9S12
Vector pUC18 Cloning site Pst I Insert size 3.1 kb
Comment Linkage analysis ASSP3 D9S1 GALT (Galactose 1-P-uridyltransferase)
Reference Human Genome Mapping 9 NAR, 16, 5216 (1988) Genomics, 3, 361 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 3.1 kb Pst I fragment from the cosmid cTHH22 was subcloned into the Pst I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Taq I (T1 : 12.0 kb, T2 : 8.0 kb). Pst I shows a two allele polymorphism showing a high degree of linkage diequilibrium with the Taq I RFLP. Not polymorphic for BamH I, Bgl II, EcoR I, Hind III, Msp I, Pvu II, Rsa I.
RDB No. HPR09010 Name of clone pEKZ19.3
Chromosome 9q Locus D9S17
Vector pUC18 Cloning site Taq I / Acc I Insert size 3.2 kb
Comment Linkage analysis ABO AK1 (Adenylate kinase 1) ABL
Reference Human Genome Mapping 9 NAR, 15, 10610 (1987) Gemomics, 3, 361 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 3.2 kb Taq I fragment into the Acc I site of pUC 18.
Host JM109 Genetic Marker Amp
RFLP Taq I (T1 : 3.2 kb, T2 : 2.5 kb). Not polymorphic for BamH I, Bgl II, EcoR I, Hind III, Msp I, Pst I, Pvu II, Rsa I.
RDB No. HPR09011 Name of clone pMHZ21
Chromosome 9 Locus
Vector pGEM3 Cloning site Hind III Insert size 2.6 kb
Comment RW-254 VNTR Linkage analysis
Reference Nucleic Acids Research, 16, 10409 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 2.6 kb Hind III fragment from the cosmid MHZ21 (isolated by the oligonucleotides of zetaglobin coding region) into Hind III site of pGEM3.
Host Genetic Marker Amp
RFLP Msp I, Taq I, Rsa I, Pst I, Pvu II (VNTR polymorphism with a band between 0.8 kb and 1.2 kb) without constant band.
RDB No. HPR10001 Name of clone pHHH105.1
Chromosome 10 Locus D10S13
Vector pUC18 Cloning site Pvu II / Hinc II Insert size 2.1 kb
Comment Linkage analysis D10S1 D10S4 IRBP (Intestitial retinol binding protein)
Reference Human Genome Mapping 9 Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 2.1 kb Pvu II fragment from the cosmid cHHH105 was subcloned into the Hinc II site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Bgl II (B1 : 9.0 kb, B2 : 8.0 kb) with a constant band at 6.0 kb. Pvu II, Pst I also appear polymorphic, but these observations have not been verified by pedigree studies. Not polymorphic for EcoR I, BamH I, Hind III.
RDB No. HPR10003 Name of clone pMCK2
Chromosome 10 Locus D10S15
Vector pUC18 Cloning site Msp I / Acc I Insert size 2.0 kb
Comment VNTR Linkage analysis D10S1 D10S4 IRBP (Intestitial retinol binding protein)
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 374 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 2.0 kb Msp I fragment from the cosmid cMCK2 [isolated by the oligonucleotides (GTGTGTGTGTGTGTGTGTGTGT) ] was subcloned into the Acc I site of pUC18.
Host HB101 Genetic Marker Amp
RFLP Rsa I, Msp I, Pst I, Pvu II (VNTR polymorphism with bands between 0.8 kb and 1.2 kb) without a constant band. Not polymorphic for Taq I, Bgl II.
RDB No. HPR10004 Name of clone pCMM17.1
Chromosome 10 Locus D10S16
Vector pUC18 Cloning site Msp I / Acc I Insert size 2.5 kb
Comment Linkage analysis D10S1 D10S4 IRBP (Intestitial retinol binding protein)
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 3121 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 2.5 kb Msp I fragment from the cosmid cCMM17 was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I (M1 : 2.3 kb, M2 : 1.5 kb) without a constant band. Not polymorphic for Taq I, Rsa I, Pst I, Bgl II, Pvu II.
RDB No. HPR10005 Name of clone pCMM17.4
Chromosome 10 Locus D10S23
Vector pUC18 Cloning site Msp I / Acc I Insert size 2.2 kb
Comment Linkage analysis D10S1 D10S4 IRBP (Intestitial retinol binding protein)
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 4190 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 2.2 kb Msp I fragment from the cosmid cCMM17 was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Pvu II (P1 : 4.3 kb, P2 : 3.2 kb) with a constant band at 2.3 kb. Not polymorphic for Taq I, Rsa I, Pst I, Bgl II, Msp I.
RDB No. HPR10006 Name of clone pMHZ15
Chromosome 10 Locus D10S17
Vector pUC18 Cloning site Msp I / Acc I Insert size 3.5 kb
Comment Linkage analysis D10S1 D10S4 IRBP (Intestitial retinol binding protein)
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 373 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 3.5 kb Msp I fragment from the cosmid cMHZ15 was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I (M1 : 3.5 kb, M2 : 2.1 kb) without a constant band. Not polymorphic for Taq I, Rsa I, Pst I, Bgl II, Pvu II.
RDB No. HPR10007 Name of clone pYNZ156
Chromosome 10 Locus D10S18
Vector pUC18 Cloning site EcoR I Insert size 4.6 kb
Comment Linkage analysis D10 S1 D10 S4 IRBP (Intestitial retinol binding protein)
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 375 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 4.6 kb EcoR I fragment from the cosmid cYNZ156 was subcloned into the EcoR I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I (M1 : 11.5 kb, M2 : 9.6 kb) without a constant band. Not polymorphic for Taq I, Rsa I, Pst I, Bgl II, Pvu II.
RDB No. HPR10008 Name of clone pTB10.171
Chromosome 10 Locus D10S19
Vector pUC18 Cloning site EcoR I Insert size 1.9 kb
Comment Linkage analysis D10S1 D10S4 IRBP (Intestitial retinol binding protein)
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 4187 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 1.9 kb EcoR I fragment from phage TB10.171 was subcloned into the EcoR I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Pvu II (P1 : 8.0 kb, P2 : 6.5 kb) without a constant band. Not polymorphic for Taq I, Rsa I, Pst I, Bgl II, Msp I.
RDB No. HPR10009 Name of clone TBQ10.163
Chromosome 10 Locus D10S22
Vector pUC18 Cloning site EcoR I Insert size 2.7 kb
Comment Linkage analysis D10S1 D10S4 IRBP (Intestitial retinol binding protein)
Reference Genomics, 2, 157 (1988) Nucleic Acids Research, 16, 4185 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 2.7 kb EcoR I fragment from phage TB10.163 was subcloned into the EcoR I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I (M1 : 15.0 kb, M2 : 12.0 kb, M3 : 8.0 kb) without a constant band. Not poolymorphic for Taq I, Rsa I, Pst I, Bgl II, Pvu II.
RDB No. HPR10010 Name of clone EFD70.2
Chromosome 10q Locus D10S26
Vector pUC18 Cloning site Pst I Insert size 2.6 kb
Comment HBV – 3 VNTR Linkage analysis
Reference Genomics, 2, 157 (1988) Nucleic Acids Research, 16, 10407 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 2.6 kb Pst I fragment from the cosmid cEFD70 (isolated by the oligonucleotides of HBV-3) was subcloned into the Pst I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Pvu II, Pst I (VNTR polymorphism with bands between 1.5 kb and 3.0 kb) without a constant band. Not polymorphic for Bgl II.
RDB No. HPR10012 Name of clone pEFD75
Chromosome 10 Locus D10S
Vector pUC18 Cloning site Taq I / Acc I Insert size 2.4 kb
Comment HBV – 3 VNTR Linkage analysis D10S1 D10S4 IRBP (Intestitial retinol binding protein)
Reference Genomics, 2, 157 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 2.4 kb Taq I fragment from the cosmid cEFD75 (isolated by the oligonucleotides of HBV-3) was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Tet
RFLP Taq I, Rsa I, Pvu II, EcoR I, Hind III, Pst I (VNTR polymorphism with bands between 2.0 kb and 3.0 kb) without constant band. Not polymorphic for Bgl II, Msp I.
RDB No. HPR11002 Name of clone pMCT128.1
Chromosome 11q Locus D11S285
Vector pUC18 Cloning site Taq I / Acc I Insert size 4.0 kb
Comment Linkage analysis PGA APOA1
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 378 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 4.0 kb Taq I fragment from the cosmid cMCT128 was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I (M1 : 2.9 kb, M2 : 2.6 kb). Not polymorphic for Bgl II, Pvu II, Taq I.
RDB No. HPR11003 Name of clone pMCT117
Chromosome 11q Locus D11S145
Vector pUC18 Cloning site Bal II / BamH I Insert size 2.4 kb
Comment Linkage analysis PGA APOA1
Reference Nucleic Acids Research, 16, 4745 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 2.4 kb Bgl II fragment from the cosmid cMCT117 was subcloned into the BamH I site of pUC18.
Host HB101 Genetic Marker Amp
RFLP Msp I (M1 : 2.9 kb, M2 : 2.7 kb, M3 : 2.5 kb). Taq I also polymorphic but this has not been examined by family studies. Not polymorphic for Bgl II, Pst I, Pvu II, Rsa I.
RDB No. HPR11004 Name of clone pHBI59
Chromosome 11 Locus D11S146
Vector pUC18 Cloning site Msp I / Acc I Insert size 1.6 kb
Comment Linkage analysis PGA
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 376 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 1.6 kb Msp I fragment from the cosmid cHB159 was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I (M1: 4.3 kb, M2 : 3.8 kb, 0.5 kb). Taq I also polymorphism but this observation has not been verified by pedigree studies. Not polymorphic for Bgl II, Pst I, Pvu II, Rsa I.
RDB No. HPR11005 Name of clone pHBI18P2
Chromosome 11 Locus D11S147
Vector pUC18 Cloning site Pst I Insert size 4.0 kb
Comment Linkage analysis PGA APOA1 D11S29
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 377 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 4.0 kb Pst I fragment from the cosmid cHBI18P2 was subcloned into the Pst I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Pst I (P1 : 5.0 kb, P2 : 4.0 kb). Not polymorphic for Pvu II, BamH I, Bgl II, EcoR I, Hind III, Msp I, Rsa I, Taq I.
RDB No. HPR11006 Name of clone pYNB3.12
Chromosome 11 Locus
Vector pUC18 Cloning site Msp I / Acc I Insert size 2.4 kb
Comment Band III Linkage analysis PGA
Reference Human Genome Mapping 9 Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 2.4 kb Msp I fragment from the cosmid cYNB3.12 (isolated with Band III cDNA) and subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I (M1 : 2.8 kb, M2 : 2.4 kb). Not polymorphic for BamH I, Bgl II, EcoR I, Hind III, Pst I, Pvu II, Rsa I, Taq I.
RDB No. HPR11007 Name of clone pTHH26
Chromosome 11 Locus D11S149
Vector pUC18 Cloning site Pvu II / Hinc II Insert size 3.0 kb
Comment Linkage analysis PGA APOA1
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 4746 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 3.0 kb Pvu II fragment from the cosmid cTHH26 was subcloned into the Hinc II site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Pvu II (P1 : 5.2 kb, P2 : 3.0 kb). Not polymorphic for BamH I, Bgl II, EcoR I, Hind III, Msp I, Pst I, Rsa I, Taq I.
RDB No. HPR12001 Name of clone p7G11
Chromosome 12 Locus D12S8
Vector pBR322 Cloning site EcoR I – Hind III Insert size 1.0 kb
Comment Linkage analysis
Reference Human Genome Mapping 9 Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description
Host HB101 Genetic Marker Amp
RFLP Msp I (M1 : 7.0 kb, M2 : 6.0 kb).
RDB No. HPR12002 Name of clone pYNH15
Chromosome 12q Locus D12S17
Vector pUC18 Cloning site Msp I / Acc I Insert size 3.2 kb
Comment Linkage analysis D12S6 D12 S4 COLL2A1 D12S2
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 779 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 3.2 kb Msp I fragment from the cosmid cYNH15 was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I (M1 : 4.0 kb, M2 : 3.2 kb, M3 : 2.6 kb). Rsa I (R1 : 3.0 kb, R2 : 1.7 kb) but this has not been tested in pedigree studies. Not polymorphic for BamH I, Bgl II, EcoR I, Hind III, Pst I, Pvu II, Taq I.
RDB No. HPR12003 Name of clone pEFD33.2
Chromosome 12 Locus
Vector pUC18 Cloning site Taq I / Acc I Insert size 3.2 kb
Comment Linkage analysis
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 778 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 3.2 kb Taq I fragment from the cosmid cEFD33 cloned into Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Taq I (A1 : 3.2 kb, A2 : 4.3 kb). Msp I (M1 : 3.2 kb, M2 : 7.0 kb, M3 : 9.0 kb). EcoR I (E1 : 6.2 kb, E2 : 6.0 kb). Not polymorphic for Rsa I, Bgl II, Pst I, Pvu II, BamH I, Hind III.
RDB No. HPR12004 Name of clone pTHIZ53
Chromosome 12 Locus D12S18
Vector pBR322 Cloning site EcoR I – Sal I / Cla I Insert size 2.3 kb
Comment Linkage analysis D12S4 COL2A1 D12S2 KRAS2
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 5701 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 2.3 kb EcoR I / Sal I fragment from the cosmid cTHIZ53 was subcloned into the Cla I site of pBR322.
Host HB101 Genetic Marker Amp
RFLP Rsa I, BamH I, Bgl II, EcoR I, Hind III, Msp I, Pst I, Pvu II, Taq I resolve the 2 allele insertion / deletion (R1 : 2.4 kb, R2 : 2.2 kb).
RDB No. HPR13007 Name of clone pMHZ17
Chromosome 13q Locus D13S51
Vector pGEM4 Cloning site Taq I / Acc I Insert size 4.2 kb
Comment Linkage analysis D13S6 D13S1 ESD D13S4
Reference Nucleic Acids Research, 16, 10401 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 4.2 kb Taq I fragment isolated from the cosmid MHZ17 has been subcloned into Acc I site of pGEM4.
Host Genetic Marker
RFLP Taq I (T1 : 4.2 kb, H2 : 3.2 kb). Not polymorphic for Msp I, Rsa I, Pst I, Pvu II, Bgl II.
RDB No. HPR13008 Name of clone pYNH20
Chromosome 13 Locus D13S50
Vector pUC18 Cloning site Hind III Insert size 4.0 kb
Comment Linkage analysis D13S6 D13S1 ESD D13S4
Reference NAR, 16, 10402 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 4.0 kb Hind III fragment isolated from the cosmid YNN20 has been subcloned into Hind III site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Hind III (H1 : 11.0 kb, H2 : 7.5 kb). Not polymorphic for Msp I, Taq I, Rsa I, Pst I, Pvu II, Bgl II.
RDB No. HPR13009 Name of clone pTHI62
Chromosome 13 Locus
Vector pBR322 Cloning site Hind III Insert size 12.0 kb
Comment VNTR Linkage analysis D13S6 D13S1 ESD D13S4
Reference Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 12.0 kb Hind III fragment from the phage THI62 (isolated by the oligonucleotides of insulin coding region) was subcloned into the Hind III site of pBR322.
Host HB101 Genetic Marker Amp
RFLP Bgl II, Msp I, Taq I, Rsa I, Pvu II, Pst I, EcoR I, BamH I, Hind III (VNTR polymorphism with allele between 6.0 kb and 9.0 kb) and a large number of non-polymorphic bands above and below the VNTR region.
RDB No. HPR14001 Name of clone pAW101
Chromosome 14 Locus D14S1
Vector pBR322 Cloning site EcoR I Insert size 5.0 kb
Comment Linkage analysis
Reference Genomics, 4, 76 (1989)
Human Genome Mapping 9 PNAS, 77, 6754 (1980)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description
Host HB101 Genetic Marker Amp
RFLP EcoR I (* major DNA fragments between 14 kb and 29 kb with many minor bands).
RDB No. HPR14002 Name of clone pEFZ18.2
Chromosome 14 Locus D14S17
Vector pUC18 Cloning site Taq I / Acc I Insert size 3.5 kb
Comment Linkage analysis
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 380 (1988)
Developer Depositor Y. Nakamura and R. White
Description
Host JM109 Genetic Marker Amp
RFLP
RDB No. HPR14004 Name of clone pTHH37
Chromosome 14 Locus D14S16
Vector pUC18 Cloning site Pst I Insert size 2.4 kb
Comment HBV – 1 Linkage analysis D14S1 GM PI
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 3593 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 2.4 kb Pst I fragment from the cosmid cTHH37 (isolated by the oligonucleotides of HBV-1) was subcloned into the Pst I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Pst I, Taq I, Msp I (P1 : 3.0 kb, P2 : 2.4 kb, P3 : 2.1 kb).
RDB No. HPR14006 Name of clone pCMM62
Chromosome 14q Locus D14S21
Vector pUC18 Cloning site Pvu II / Hinc II Insert size 3.1 kb
Comment Linkage analysis GM D14S1 PI
Reference Nucleic Acids Research, 16, 5220 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 3.1 kb Pvu II fragment from the cosmid cCMM62 was subcloned into the Hinc II site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Hind III (H1 : 4.0 kb, H2 : 2.5 kb). Not polymorphic for Msp I, Taq I, Bgl II, Pst I, Pvu II, Rsa I.
RDB No. HPR14007 Name of clone pCMM66
Chromosome 14q Locus D14S22
Vector pUC18 Cloning site Taq I / Acc I Insert size 4.8 kb
Comment Myoglobin VNTR Linkage analysis D14S1 GM PI
Reference Nucleic Acids Research, 16, 6255 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 4.8 kb Taq I fragment from the cosmid cCMM66 isolated by the oligonucleotides of myoglobin-2 coding regin was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Pst I, Rsa I (VNTR polymorphism with a band between 4.0 kb and 6.0 kb) with constant band at 1.5 kb and 1.2 kb. Not polymorphic for Rsa I, Taq I.
RDB No. HPR14008 Name of clone pMCOC12
Chromosome 14 Locus D14S20
Vector pUC18 Cloning site Pst I Insert size 3.4 kb
Comment VNTR Linkage analysis D14S1 GM PI
Reference Nucleic Acids Research, 16, 6257 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura and R. White
Description A 3.4 kb Pst I fragment from the cosmid cMCOC12 [isolated by the oligonucleotides (GAGGGTGGGGGNTCNNNG) ] was subcloned into the Pst I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I, Rsa I, Taq I, Hae III, Pst I (VNTR polymorphism with a band between 1.0 kb and 2.0 kb).
RDB No. HPR14009 Name of clone pCMM101
Chromosome 14 Locus
Vector pUC18 Cloning site Msp I / Acc I Insert size 2.2 kb
Comment Myoglobin – 2 Linkage analysis
Reference Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 2.2 kb Msp I fragment from the cosmid cCMM101 (isolated by the oligonucleotides of myoglobin-2 coding region) cloned into Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I, Taq I, Rsa I, Bgl II, Pst I (> 10 allele polymorphism with a band between 1.5 kb and 6.0 kb without constant band).
RDB No. HPR15001 Name of clone pTHH114
Chromosome 15q Locus D15S25
Vector pUC18 Cloning site Rsa I / Hinc II Insert size 2.4 kb
Comment Linkage analysis D15S1 D15S2 D15S3
Reference Human Genome Mapping 9 Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 2.4 kb Rsa I fragment from the cosmid cTHH114 was subcloned into the Hinc II site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Rsa I (R1 : 2.5 kb, R2 : 2.4 kb). Not polymorhic for BamH I, Bgl II, EcoR I, Hind III, Msp I, Pst I, Pvu II, Taq I.
RDB No. HPR15002 Name of clone pMCT46.2
Chromosome 15q Locus D15S26
Vector pUC18 Cloning site BamH I Insert size 5.3 kb
Comment Linkage analysis D15S1 D15S2 D15S3
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 1226 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 5.3 kb BamH I fragment from the cosmid cMCT46 was subcloned into the BamH I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Pvu II (P1 : 5.9 kb, P2 : 5.3 kb). Not polymorphic fore Msp I, TaqU I, Rsa I, Bgl II, Pst I.
RDB No. HPR15003 Name of clone pTHH55
Chromosome 15q Locus D15S27
Vector pUC18 Cloning site BamH I Insert size 7.0 kb
Comment Linkage analysis D15S1 D15S2 D15S3
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 10945 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 7.0 kb BamH I fragment from the cosmid cTHH55 was subcloned into the BamH I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I (M1 : 4.5 kb, M2 : 3.3 kb, M3 : 1.2 kb) with constant bands at 2.6 kb, 0.85 kb, 0.80 kb. Not polymorphic for BamH I, Bgl II, EcoR I, Hind III, Rsa I, Taq I.
RDB No. HPR15004 Name of clone pYNZ90 (pYNZ90.1)
Chromosome 15 Locus D15S28
Vector pUC18 Cloning site BamH I Insert size 6.0 kb
Comment Linkage analysis D15S2 D15S1 D15S3
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 780 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 6.0 kb BamH I fragment from the cosmid cYNZ90 was subcloned into the BamH I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP BamH I (B1 : 6.0 kb, B2 : 5.8 kb). Bgl II, Rsa I appear to be polymorphic, but, this observation has not been verified by pedigree studies. Not polymorphic for EcoR I, Hind III, Msp I, Pst I, Pvu II, Taq I.
RDB No. HPR15005 Name of clone pEFD49.3
Chromosome 15q Locus
Vector pUC18 Cloning site Msp I / Acc I Insert size 2.0 kb
Comment Linkage analysis D15S1 D15S2 D15S3
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 10944 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 2.0 kb Msp I fragment from the cosmid cEFD49 was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I (M1 : 3.0 kb, M2 : 2.1 kb, M3 : 2.0 kb) without a constant band. Taq I (T1 : 2.45 kb, T2 : 2.1 kb, T3 : 2.0 kb) without a constant band. Not polymorphic for Rsa I.
RDB No. HPR15006 Name of clone pEFD49.2
Chromosome 15 Locus D15S38
Vector pUC18 Cloning site Msp I / Acc I Insert size 2.8 kb
Comment Linkage analysis D15S1 D15S2 D15S3
Reference Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 2.8 kb Msp I fragment from the cosmid cEFD49 was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Taq I (T1: 4.4 kb, T2 : 2.3kb, T3 : 2.1 kb) without a constant band. Msp I (M1 : 3.7 kb, M2 : 2.8 kb). Not polymorphic for Rsa I.
RDB No. HPR15007 Name of clone pEKZ104.1
Chromosome 15q Locus D15S30
Vector pUC18 Cloning site Msp I / Acc I Insert size 4.0 kb
Comment Linkage analysis D15S1 D15S2 D15S3
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 1224 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 4.0 kb Msp I fragment from the cosmid cEKZ104 was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I (M1 : 4.0 kb, M2 : 3.7 kb). Bgl II, EcoR I also appear polymorphic, but these observation has been not verified by pedigree studies.
RDB No. HPR15008 Name of clone pMCA1-1
Chromosome 15q Locus D15S33
Vector pUC18 Cloning site Pvu II / Hinc II Insert size 4.6 kb
Comment Linkage analysis D15S1 D15S2 D15S3
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 1225 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 4.6 kb Pvu II fragment from the cosmid cMCA1 – 1 was subcloned into the Hinc II site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Pvu II (P1 : 5.7 kb, P2 : 5.2 kb). Not polmorphic for BamH I, Bgl II, EcoR I, Hind III, Pst I, Rsa I, Taq I.
RDB No. HPR15010 Name of clone pMCT149.2
Chromosome 15q Locus D15S34
Vector pUC18 Cloning site Msp I / Acc I Insert size 3.0 kb
Comment Linkage analysis D15S1 D15S2 D15S3
Reference Genomics, 3, 342-346 (1988) Nucleic Acids Research, 16, 10941 (1988) Developer Depositor Y. Nakamura and R. White
Description A 3.0 kb Msp I fragment from the cosmid cMCT149 was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I (M1 : 3.0 kb, M2 : 2.0 kb) without a constant band. Taq I (T1 : 2.4 kb, T2 : 1.2 kb) without a constant band, but this has not verified by our three generation families. Not polymorphic for Rsa I.
RDB No. HPR15011 Name of clone pEFD85.7
Chromosome 15q Locus D15S37
Vector pUC18 Cloning site EcoR I Insert size 2.4 kb
Comment Linkage analysis D15S1 D15S2 D15S3
Reference Gemomics, 3, 342-346 (1988) Nucleic Acids Research, 16, 6256 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 2.4 kb EcoR I fragment from the cosmid cEFD85 was subcloned into the EcoR I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP EcoR I (E1: 4.3 kb, E2 : 2.4 kb) without a constant band. Not polymorphic for BamH I, Bgl II, Msp I, Hind III, Rsa I, Taq I.
RDB No. HPR15012 Name of clone pEFD52.1
Chromosome 15q Locus D15S44
Vector pUC18 Cloning site Taq I / Acc I Insert size 2.3 kb
Comment Linkage analysis D15S1 D15S2 D15S3
Reference Genomics, 3, 342-346 (1988) Nucleic Acids Research, 16, 10942 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 2.3 kb Taq I fragment from the cosmid cEFD52 was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I (M1 : 2.1 kb, M2 : 1.4 kb + 0.7 kb) without a constant band. Not polymorphic for Taq I, Rsa I.
RDB No. HPR15013 Name of clone pYNM18.1
Chromosome 15q Locus D15S35
Vector pUC18 Cloning site Pst I Insert size 6.6 kb
Comment Linkage analysis D15S1 D15S2 D15S3
Reference Genomics, 3, 342-346(1988) Nucleic Acids Research, 16, 3592 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 6.6 kb Pst I fragment from the cosmid cYNM18 was subcloned into the Pst I site of pUC18.
Host Genetic Marker Amp
RFLP Taq I (T1 : 4.8 kb, T2 : 4.4 kb, T3 : 4.3 kb) with constant bands at 2.0 kb, 4.0 kb. Msp I (M1 : 3.3 kb, M2 : 2.6 kb) with constant bands at 0.8 kb, 0.7 kb, but this has not verified by our three generation families . Not polymorphic for Rsa I, Bgl II, Pvu II, Pst I.
RDB No. HPR15014 Name of clone pEFZ33
Chromosome 15q Locus D15S45
Vector pUC18 Cloning site EcoR I / Acc I Insert size 2.3 kb
Comment Linkage analysis D15S1 D15S2 D15S3
Reference Genomics, 3, 342-346(1988) Nucleic Acids Research, 16, 10946 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 2.3 kb EcoR I fragment from the cosmid cEFZ33 was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Hind III (H1 : 4.0 kb, H2 : 3.7 kb) without a constant band.
RDB No. HPR16001 Name of clone pEKXp3B
Chromosome 16 Locus
Vector Okayama – Berg expression vector Cloning site Xho I Insert size 1.1 kb
Comment Linkage analysis
Reference Human Genome Mapping 9 Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description
Host Genetic Marker Amp
RFLP BamH I, EcoR I, Pvu II, Bgl II, Hind III, Taq I.
RDB No. HPR16002 Name of clone pCMM65
Chromosome 16 Locus D16S84
Vector pUC18 Cloning site Rsa I / Hinc II Insert size 3.2 kb
Comment Myoglobin – 2 Linkage analysis HBZP1
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 3122 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 3.2 kb Rsa I fragment from the cosmid cCMM65 (isolatid by the oligonucleotides of myoglobin-2) was subcloned into the Hinc II site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I, Taq I, Rsa I, Bgl II, Pst I, EcoR I, BamH I, Hind III, Pvu II (VNTR polymorphism with a band at 1.5 kb or 1.3 kb) without a constant band.
RDB No. HPR16003 Name of clone pEKMDA2-I
Chromosome 16 Locus D16S83
Vector pUC18 Cloning site Rsa I / Hinc II Insert size 3.5 kb
Comment VNTR Linkage analysis HBZP1
Reference Nucleic Acids Research, 16, 9885 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 3.5 kb Rsa I fragment from the cosmid cEKMDA2 was subcloned into the Hinc II site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Hinf I, Taq I, Rsa I, Bgl II, Pst I, EcoR I, BamH I, Hind III, Pvu II (VNTR polymorphism with bands between 0.8 kb and 1.5 kb).
RDB No. HPR16004 Name of clone pMCOC36
Chromosome 16 Locus
Vector pUC18 Cloning site Bal II / BamH I Insert size 6.4 kb
Comment VNTR RW – 304 Linkage analysis
Reference Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 6.4 kb Bgl II fragment from the cosmid cMCO36 (isolated by the oligonucleotides) cloned into BamH I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I, Rsa I, Taq I, Hae III, Pst I (M1 : 1.1 kb, M2 : 2.0 kb) without a constamt band.
RDB No. HPR16005 Name of clone pKKA22
Chromosome 16 Locus
Vector pUC18 Cloning site Msp I / Acc I Insert size 2.8 kb
Comment VNTR RW – 332 Linkage analysis
Reference Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 2.8 kb Msp I fragment fron the cosmid cKKA22 cloned into Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Pst I (A1 : 13.0 kb, A2 : 6.0 kb, A3: 4.4 kb) with constant band at 7.0 kb. Not polymorphic for Msp I, Taq I, Rsa I, Pvu II, Bgl II.
RDB No. HPR17003 Name of clone pRMU3
Chromosome 17 Locus D17S24
Vector pUC18 Cloning site BamH I-Sac I Insert size 2.0 kb
Comment Linkage analysis
Reference Nucleic Acids Research, 16, 784 (1988) Developer Depositor
Description
Host Genetic Marker
RFLP
RDB No. HPR17005 Name of clone pRMU1
Chromosome 17q Locus D17S27
Vector pUC18 Cloning site BamH I Insert size 2.0 kb
Comment Linkage analysis D17Z1 TK1 pTHH59
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 785 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 2.0 kb BamH I fragment from the cosmid cRMU1 was subcloned into the BamH I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Pst I (P1 : 2.0 kb, P2 : 1.0 kb) with a constant band at 4.0 kb. Not polymorphic for Msp I, Taq I, Rsa I, EcoR I, Hind III, BamH I, Pvu II, Bgl II.
RDB No. HPR17006 Name of clone pHHH152
Chromosome 17q Locus D17S32
Vector pUC18 Cloning site BamH I Insert size 7.0 kb
Comment Linkage analysis D17Z1 TK1
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 787 (1988)
Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 7.0 kb BamH I fragment from the cosmid cHHH152 subcloned into BamH I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP BamH I (B1 : 10.5 kb, B2 : 9.6 kb) with a constant band at 2.6 kb. Not polymorphic for Bgl II, EcoR I, Hind III, Msp I, Pst I, Pvu II, Rsa I, Taq I.
RDB No. HPR17008 Name of clone pCMM86
Chromosome 17p Locus D17S74
Vector pUC18 Cloning site Msp I / Acc I Insert size 4.3 kb
Comment Myoglobin VNTR Linkage analysis D17S1 MYH2 D17Z1
Reference Human Genome Mapping 9
Nucleic Acids Research, 16, 5223 (1988)
Developer Depositor Y. Nakamura and R. White
Description A 4.3 kb Msp I fragment from the cosmid cCNN86 (isolated by the oligonucleotides of myoglobin-2) was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Hinf I, Taq I, Msp I, Rsa I, Bgl II (VNTR polymorphism with bands between 1.0 kb and 5.0 kb). Not polymorphic for Pst I.
RDB No. HPR17009 Name of clone pJCZ16.2
Chromosome 17 Locus
Vector pUC18 Cloning site Pst I Insert size 4.3 kb
Comment Zetaglobin, VNTR, RW – 208 Linkage analysis
Reference Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 4.3 kb Pst I fragment from the cosmid cJCZ3 (isolated by the oligonucleotides of zetaglobin) cloned into Pst I site of pUC18.
Host Genetic Marker Amp
RFLP Taq I, Hinf I, Msp I, Rsa I, Bgl II, Pst I, Pvu II, BamH I (VNTR polymorphism between 2.0 kb and 3.3 kb).
RDB No. HPR18001 Name of clone pMS1-3
Chromosome 18q Locus D18S19
Vector pUC18 Cloning site EcoR I – Hind III Insert size 1.3 kb
Comment Linkage analysis
Reference Human Genome Mapping 9 Genomics, 3, 367 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description
Host JM109 Genetic Marker Amp
RFLP Pst I, Bcl I, BstN I Not polymorphic for Mbo I, Msp I, Taq I, EcoR I, Hind III, BamH I, Pvu II, Bgl II Hinc II, Nci I, Sst I, Xma I, Hae III, Acc I, Rsa I, EcoR V, Alu I, Sin I, Hinf I, Xba I, Dde I, Apa I, Nco I, Tth I, Xma I, Bst I,Taq II.
RDB No. HPR18002 Name of clone pEFZ10
Chromosome 18 Locus D18S20
Vector pUC18 Cloning site Pvu II / Hinc II Insert size 3.8 kb
Comment VNTR Linkage analysis D18S1 D18S3
Reference Human Genome Mapping 9 NAR, 16, 1227 (1988) Genomics, 3, 367 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 3.8 kb Pvu II fragment from the cosmid cEFZ10 was subcloned into the Hinc II site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Pvu II, Pst I (VNTR polymorphism with bands between 2.5 kb and 5.0 kb) with a constant band at 2.0 kb. Not polymorphic for Msp I, Bgl II.
RDB No. HPR18003 Name of clone pHHH163
Chromosome 18 Locus D18S21
Vector pUC18 Cloning site EcoR I Insert size 3.2 kb
Comment Linkage analysis D18S1 D18S3
Reference Human Genome Mapping 9 NAR, 16, 4189 (1988) Genomics, 3, 367 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 3.2 kb EcoR I fragment from the cosmid cHHH163 was subcloned into the EcoR I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Pvu II (P1 : 4.7 kb, P2 : 2.8 kb) without a constant band. Not polymorphic for Msp I, Taq I, Rsa I, Pst I, EcoR I, Hind III, BamH I, Bgl II.
RDB No. HPR18004 Name of clone pMCT108.2
Chromosome 18 Locus D18S24
Vector pUC18 Cloning site Msp I Insert size 3.2 kb
Comment Linkage analysis D18S1 D18S3
Reference Genomics, 3, 367 (1988) Nucleic Acids Research, 16, 4188 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 3.2 kb Msp I fragmen from MCT108 was subcloned into the Msp I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I (M1 : 3.2 kb, M2 : 2.3 kb) without a constant band. Taq I (T1 : 3.8 kb,T2 : 2.3 kb) but this has noty veified with verifiedfamily studies. Not polymorphic for Rsa I, Pst I, Pvu II, Bgl II.
RDB No. HPR19001 Name of clone pJCZ3.1
Chromosome 19 Locus D19S20
Vector pUC18 Cloning site Pst I Insert size 4.5 kb
Comment Zetaglobin VNTR Linkage analysis LDLA APOC2
Reference Human Genome Mapping 9 Genomics, 3, 67-71 (1988) NAR, 16, 1229 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 4.5 kb Pst I fragment from the cosmid cJCZ3 (isolatid by the oligonucleotides of zetaglobin) was subcloned into the Pst I site of pUC 18.
Host JM109 Genetic Marker Amp
RFLP Hinf I, Msp I, Taq I, Rsa I, Bgl II, Pst I, Pvu II (VNTR polymorphism with bands between 1.5 kb and 4.0 kb) without a constant band.
RDB No. HPR19004 Name of clone pMCT6
Chromosome 19 Locus D19S24
Vector pUC18 Cloning site BamH I- EcoR I Insert size 7.7 kb
Comment Dora, RW – 10 Linkage analysis
Reference Human Genome Mapping 9 Genomics, 3, 67-71(1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description
Host JM109 Genetic Marker Amp
RFLP Bgl II, Msp I, Taq I, Rsa I, Bgl II, Pst I, Pvu II (9-3 kb). EcoR I (5.2 kb, 5.0 kb, 4.3 kb). BamH I (20 kb, 15.5 kb, 14.7 kb, 10.4 kb, 5.3 kb, 5.2 kb, 3.4 kb, 2.2kb).
RDB No. HPR20001 Name of clone pMS1-27
Chromosome 20 Locus D20S4 (D0SLC2)
Vector pBR322 Cloning site EcoR I – Hind III Insert size 1.2 kb
Comment Linkage analysis
Reference Human Genome Mapping 9 Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description
Host HB101 Genetic Marker Amp
RFLP Msp I (M1 : 1.5 kb, M2 : 6.5 kb). Not polymorphic for Mbo I, Taq I, EcoR I, Hind III, BamH I, Kpn I, Pvu II, Hinc II, Pst I, Nci I, Sst I, Xma I, Hae III, Bst N I, BgtE II,Acc I, Stu I, Rsa I, EcoR V, Alu I, Sin I, Hinf I, Xba I, Sau3A, Dde I, Nae I.
RDB No. HPR20002 Name of clone pCMM6
Chromosome 20 Locus D20S19
Vector pUC18 Cloning site Pst I Insert size 4.0 kb
Comment Myoglobin VNTR Linkage analysis D20S1
Reference Nucleic Acids Research, 16, 5222 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 4.0 kb Pst I fragment from the cosmid cCMM6 (isolated by the oligonucleotides of myoglobin-1) was subcloned into the Pst I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Taq I, Msp I, Rsa I, Pvu II, Pst I (VNTR polymorphism with a band between 2.3 kb and 6.0 kb).
RDB No. HPR20003 Name of clone pRMR6
Chromosome 20 Locus D20S20
Vector pUC18 Cloning site Bgl II / BamH I Insert size 7.5 kb
Comment Linkage analysis D20S1
Reference Nucleic Acids Research, 16, 9883 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 7.5 kb Bgl II fragment from the cosmid cRMR6 was subcloned into the BamH I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Taq I (T1 : 2.5 kb, T2 : 2.3 kb) with a constant band at 1.5 kb. Not polymorphic for Msp I, Rsa I, Bgl II, Pst I, Pvu II, EcoR I, BamH I, Hind III.
RDB No. HPR21001 Name of clone pMCT15
Chromosome 21 Locus D21S113
Vector pUC18 Cloning site Taq I / Acc I Insert size 2.7 kb
Comment Linkage analysis
Reference Nucleic Acids Research, 16, 9882 (1988) Developer Depositor Y. Nakamura and R. White
Description A 2.7 kb TaqI fragment from the cosmid cMCT15 [isolated by the oligonucleotides (GTGTGTGTGTGTGTGTGTGTGT) ] was subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I, BamH I, Pst I, Pvu II, Taq I (VNTR polymorphism with bands between 1.2 kb and 1.6 kb).
RDB No. HPR21002 Name of clone pEFD70.3
Chromosome 21 Locus D21S412
Vector pUC18 Cloning site Pst I Insert size 1.3 kb
Comment Linkage analysis D18S1 D18S3
Reference Hum. Mol. Genet. 2, 331 (1993) Developer Y. Nakamura and R. White Depositor Y. Nakamura and R. White
Description A 1.3 kb Pst I fragment from the cosmid cEFD70 was subcloned into the Pst I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Pst I (P1 : 1.5 kb, P2 : 1.3 kb). Not polymorphic for Msp I, Taq I, Rsa I, Pvu II, EcoR I, Hind III, BamH I, Bgl II.
RDB No. HPR22001 Name of clone pEFDZ31
Chromosome 22 Locus D22S32
Vector pUC18 Cloning site Msp I / Acc I Insert size 3.1 kb
Comment Linkage analysis D21S1
Reference Nucleic Acids Research, 16, 5221 (1988) Developer Y. Nakamura and R. White Depositor Y. Nakamura
Description A 3.1 kb Msp I fragment from the cosmid EFZ31 subcloned into the Acc I site of pUC18.
Host JM109 Genetic Marker Amp
RFLP Msp I (M1 : 3.5 kb, M2 : 2.6 kb). Not polymorphic for Rsa I, Bgl II, Pst I, Pvu II, BamH I, Hind III, EcoR I.