Genetic Resources for Optogenetics Research

Optogenetics resource

Light-induced silencing of neural activity in Rosa26 knock-in and BAC transgenic mice conditionally expressing the microbial halorhodopsin eNpHR3.

Imayoshi, I., Tabuchi, S., Matsumoto, M., Kitano, S., Miyachi, H., Kageyama, R., Yamanaka, A.
Sci. Rep. 10 (1): 3191 (2020). PubMed PMID 32081938.

Mouse resource

Catalog No. Name of resource
RBRC05159 B6;129S6-Gt(ROSA)26Sor<tm1(CAG-eNpHR3/EYFP)Imayo>
RBRC05160 B6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-eNpHR3/EYFP)Imayo>
RBRC05161 B6;129S6-Gt(ROSA)26Sor<tm1.2(CAG-eNpHR3/EYFP)Imayo>
RBRC06583 C57BL/6-Tg(Gt(ROSA)26Sor-eNpHR3/EYFP)1Imayo
RBRC06584 C57BL/6-Tg(Gt(ROSA)26Sor-eNpHR3/EYFP)1.1Imayo

Cholecystokinin-Expressing Interneurons of the Medial Prefrontal Cortex Mediate Working Memory Retrieval.

Nguyen, R., Venkatesan, S., Binko, M., Bang, J.Y., Cajanding, J.D., Briggs, C., Sargin, D., Imayoshi, I., Lambe, E.K., Kim, J.C.
J. Neurosci. 40 (11): 2314-2331 (2020). PubMed PMID 32005764

Mouse resource

Catalog No. Name of resource
RBRC06585 C57BL/6-Tg(Gt(ROSA)26Sor-ArchT/EGFP)1Imayo
RBRC06586 C57BL/6-Tg(Gt(ROSA)26Sor-ArchT/EGFP)1.1Imayo
RBRC06587 C57BL/6-Tg(Gt(ROSA)26Sor-ArchT/EGFP)1.2Imayo

Organelle Optogenetics: Direct Manipulation of Intracellular Ca2+ Dynamics by Light.

Asano, T., Igarashi, H., Ishizuka, T., Yawo, H.
Front. Neurosci. 12: 561 (2018). PubMed PMID 30174581.

DNA resource

Catalog No. Name of resource description
RDB16748 pCAGGS-ChRGR-Venus Expression vector of organelle optogenetics channelrhodopsin.
RDB16750 pCAGGS-ChRGR(ER)-Venus Expression vector of organelle optogenetics channelrhodopsin.

Expression vector of the channelrhodopsin-green receiver (ChRGR) (cat# RDB16748) which induces membrane potential oscillation by green light irradiation.
Expression vector of the ChRGR(ER) (cat# RDB16750) which localizes ChRGR to endoplasmic/sarcoplasmic reticulum (ER/SR). ChRGR(ER) is used as a tool to manipulation of intracellular calcium ion release.


A Chimera Na+-Pump Rhodopsin as an Effective Optogenetic Silencer.

Hoque, M.R., Ishizuka, T., Inoue, K., Abe-Yoshizumi, R., Igarashi, H., Mishima, T., Kandori, H., Yawo, H.
PLoS One 11 (11): e0166820 (2016). PubMed PMID 27861619.

DNA resource

Catalog No. Name of resource description
RDB16777 pCMV-KR2-3.0-EYFP Mammalian expression vector encoding a light-driven sodiun ion pump from Krokinobacter eikastus (KR2) fused with a membrane targeting signal (TS), eYFP, and an ER export signal (ERex) at the C-terminus under CMV promoter.
RDB16778 pCaMKIIα-KR2-3.0-EYFP-WPRE Mammalian expression vector encoding a light-driven sodiun ion pump from Krokinobacter eikastus (KR2) fused with a membrane targeting signal (TS), eYFP, and an ER export signal (ERex) at the C-terminus under CaMKIIα promoter.
RDB16779 pCMV-IaNaR-3.0-EYFP Mammalian expression vector encoding a light-driven sodiun ion pump from Indibacter alkaliphilus (IaNaR) fused with a membrane targeting signal (TS), eYFP, and an ER export signal (ERex) at the C-terminus under CMV promoter.
RDB16780 pCMV-I1K6NaR-3.0-EYFP Mammalian expression vector encoding a chimeric NaR between IaNaR and KR2 fused with a membrane targeting signal (TS), eYFP, and ER export signal (ERex) at the C-terminus under CMV promoter.
RDB16781 pCMV-I2K5NaR-3.0-EYFP Mammalian expression vector encoding a chimeric NaR between IaNaR and KR2 fused with a membrane targeting signal (TS), eYFP, and ER export signal (ERex) at the C-terminus under CMV promoter.
RDB16782 pCMV-I3K4NaR-3.0-EYFP Mammalian expression vector encoding a chimeric NaR between IaNaR and KR2 fused with a membrane targeting signal (TS), eYFP, and ER export signal (ERex) at the C-terminus under CMV promoter.
RDB16783 pCMV-I4K3NaR-3.0-EYFP Mammalian expression vector encoding a chimeric NaR between IaNaR and KR2 fused with a membrane targeting signal (TS), eYFP, and ER export signal (ERex) at the C-terminus under CMV promoter.
RDB16784 pCMV-I5K2NaR-3.0-EYFP Mammalian expression vector encoding a chimeric NaR between IaNaR and KR2 fused with a membrane targeting signal (TS), eYFP, and ER export signal (ERex) at the C-terminus under CMV promoter.
RDB16785 pCMV-I6K1NaR-3.0-EYFP Mammalian expression vector encoding a chimeric NaR between IaNaR and KR2 fused with a membrane targeting signal (TS), eYFP, and ER export signal (ERex) at the C-terminus under CMV promoter.

The regulatory mechanism of ion permeation through a channelrhodopsin derived from Mesostigma viride (MvChR1).

Watanabe, S., Ishizuka, T., Hososhima, S., Zamani, A., Hoque, M.R., Yawo, H.
Photochem. Photobiol. Sci. 15 (3): 365-374 (2016). PubMed PMID 26853505.

DNA resource

Catalog No. Name of resource description
RDB16786 pCMV-MvChR1-Venus Mammalian expression vector encoding Met1-Arg323 of channelrhodopsin 1 from Mesostigma viride (MvChR1) tagged with Venus at the C-terminus under CMV promoter.
RDB16787 pCMV-eMvChR1#1-Venus Mammalian expression vector encoding an N-terminal variant of MvChR1 (eMvChR1#1) tagged with Venus at the C-terminus under CMV promoter.
RDB16788 pCMV-eMvChR1#2-Venus Mammalian expression vector encoding an N-terminal variant of MvChR1 (eMvChR1#2) tagged with Venus at the C-terminus under CMV promoter.
RDB16789 pCMV-eMvChR1#2(A97D)-Venus Mammalian expression vector encoding an N-terminal variant of MvChR1 (eMvChR1#2) tagged with Venus at the C-terminus under CMV promoter. A97D mutation was introduced.
RDB16790 pCMV-eMvChR1#2(A97E)-Venus Mammalian expression vector encoding an N-terminal variant of MvChR1 (eMvChR1#2) tagged with Venus at the C-terminus under CMV promoter. A97E mutation was introduced.
RDB16791 pCMV-eMvChR1#2(A97Q)-Venus Mammalian expression vector encoding an N-terminal variant of MvChR1 (eMvChR1#2) tagged with Venus at the C-terminus under CMV promoter. A97Q mutation was introduced.
RDB16792 pCMV-eMvChR1#2(A97R)-Venus Mammalian expression vector encoding an N-terminal variant of MvChR1 (eMvChR1#2) tagged with Venus at the C-terminus under CMV promoter. A97R mutation was introduced.

Near-infrared (NIR) up-conversion optogenetics.

Hososhima, S., Yuasa, H., Ishizuka, T., Hoque, M.R., Yamashita, T., Yamanaka, A., Sugano, E., Tomita, H., Yawo, H.
Sci. Rep. 5: 16533 (2015). PubMed PMID 26552717.

DNA resource

Catalog No. Name of resource description
RDB16793 pCMV-C1V1-Venus Mammalian expression vector encoding a chimeric channelrhodopsin (C1V1) tagged with Venus at the C-terminus under CMV promoter.
RDB16794 pCMV-PsChR-Venus Mammalian expression vector encoding a channelrhodopsin from Platymonas (Tetraselmis) subcordiformis (PsChR) tagged with Venus at the C-terminus under CMV promoter.
RDB16795 pCaMKIIα-C1V1-Venus Mammalian expression vector encoding a chimeric channelrhodopsin (C1V1) tagged with Venus at the C-terminus under CaMKIIa promoter.
RDB16796 pCaMKIIα-PsChR-Venus Mammalian expression vector encoding a channelrhodopsin from Platymonas (Tetraselmis) subcordiformis (PsChR) tagged with Venus at the C-terminus under CaMKIIa promoter.

Structural basis for Na(+) transport mechanism by a light-driven Na(+) pump.

Kato, H.E., Inoue, K., Abe-Yoshizumi, R., Kato, Y., Ono, H., Konno, M., Hososhima, S., Ishizuka, T., Hoque, M.R., Kunitomo, H., Ito, J., Yoshizawa, S., Yamashita, K., Takemoto, M., Nishizawa, T., Taniguchi, R., Kogure, K., Maturana, A.D., Iino, Y., Yawo, H., Ishitani, R., Kandori, H., Nureki, O.
Nature 521 (7550): 48-53 (2015). PubMed PMID 25849775.

DNA resource

Catalog No. Name of resource description
RDB16777 pCMV-KR2-3.0-EYFP Mammalian expression vector encoding a light-driven sodiun ion pump from Krokinobacter eikastus (KR2) fused with a membrane targeting signal (TS), eYFP, and an ER export signal (ERex) at the C-terminus under CMV promoter.
RDB16778 pCaMKIIα-KR2-3.0-EYFP-WPRE Mammalian expression vector encoding a light-driven sodiun ion pump from Krokinobacter eikastus (KR2) fused with a membrane targeting signal (TS), eYFP, and an ER export signal (ERex) at the C-terminus under CaMKIIa promoter.

Optogenetic manipulation of activity and temporally controlled cell-specific ablation reveal a role for MCH neurons in sleep/wake regulation.

Tsunematsu, T., Ueno, T., Tabuchi, S., Inutsuka, A., Tanaka, K.F., Hasuwa, H., Kilduff, T.S., Terao, A., Yamanaka, A.
J. Neurosci. 34 (20): 6896-6909 (2014). PubMed PMID 24828644.

Mouse resource

Catalog No. Name of resource
RBRC05843 B6.Cg-Actb<tm1(tetO-ChR2*E123T/T159C)Ahky>

See also
December 2013 Mouse of the Month. New reporter line for optogenetic stimulation at high frequencies


Long-lasting silencing of orexin/hypocretin neurons using archaerhodopsin induces slow-wave sleep in mice.

Tsunematsu, T., Tabuchi, S., Tanaka, K.F., Boyden, E.S., Tominaga, M., Yamanaka, A.
Behav. Brain Res. 255: 64-74 (2013). PubMed PMID 23707248.

Mouse resource

Catalog No. Name of resource
RBRC05842 C57BL/6-Tg(tetO-ArchT/EGFP)1Ahky

Expanding the repertoire of optogenetically targeted cells with an enhanced gene expression system.

Tanaka, K.F., Matsui, K., Sasaki, T., Sano, H., Sugio, S., Fan, K., Hen, R., Nakai, J., Yanagawa, Y., Hasuwa, H., Okabe, M., Deisseroth, K., Ikenaka, K., Yamanaka, A.
Cell Rep. 2 (2): 397-406 (2012). PubMed PMID 22854021.

Mouse resource

Catalog No. Name of resource
RBRC05453 B6(Cg)-Tg(tetO-ChR2*C128S/EYFP)1Kftnk
RBRC05454 B6;129B6(Cg)-Actb<tm1(tetO-ChR2*C128S/EYFP)Kftnk>

See also
April 2013 Mouse of the MonthExpanding the repertoire of optogenetic control


Light-induced silencing of neural activity in Rosa26 knock-in mice conditionally expressing the microbial halorhodopsin eNpHR2.0.

Imayoshi, I., Tabuchi, S., Hirano, K., Sakamoto, M., Kitano, S., Miyachi, H., Yamanaka, A., Kageyama, R.
Neurosci. Res. 75 (1): 53-58 (2013). PubMed PMID 22465523.

Mouse resource

Catalog No. Name of resource
RBRC05150 B6;129S6-Gt(ROSA)26Sor<tm1(CAG-eNpHR2/EYFP)Imayo>
RBRC05151 B6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-eNpHR2/EYFP)Imayo>
RBRC05152 B6;129S6-Gt(ROSA)26Sor<tm1.2(CAG-eNpHR2/EYFP)Imayo>

Optically controlled contraction of photosensitive skeletal muscle cells.

Asano, T., Ishizua, T., Yawo, H..
Biotechnolm Bioeng. 2012 109(1): 199-204 (2012). PubMed PMID 21809334.

Cell resource

Catalog No. Name of resource description
RCB4693 ChR2-C2C12 C2C12 cells stably expressing ChR2

Opto-current-clamp actuation of cortical neurons using a strategically designed channelrhodopsin.

Wen, L., Wang, H., Tanimoto, S., Egawa, R., Matsuzaka, Y., Mushiake, H., Ishizuka, T., Yawo, H.
PLoS One 5 (9): e12893 (2010). PubMed PMID 20886118.

DNA resource

Catalog No. Name of resource description
RDB16775 pCMV-chopGR-Venus Mammalian expression vector encoding a chimeric channelopsin (channelopsin/green-receiver, chopGR) between chop1 and chop2 from Chlamydomonas reinhardtii, tagged with Venus at the C-terminus under CMV promoter.
RDB16776 pCMV-ChRGR-Venus Mammalian expression vector encoding a mammalian codon-optimized chopGR tagged with Venus at the C-terminus under CMV promoter.

Molecular determinants differentiating photocurrent properties of two channelrhodopsins from Chlamydomonas.

Wang, H., Sugiyama, Y., Hikima, T., Sugano, E., Tomita, H., Takahashi, T., Ishizuka, T., Yawo, H.
J. Biol. Chem. 284 (9): 5685-5696 (2009). PubMed PMID 19103605.

DNA resource

Catalog No. Name of resource description
RDB16769 pCMV-chop1-Venus Mammalian expression vector encoding channelopsin-1 (ChR1) from Chlamydomonas reinhardtii, tagged with Venus at the C-terminus under CMV promoter.
RDB16770 pCMV-chop2-Venus Mammalian expression vector encoding channelopsin-2 (ChR2) from Chlamydomonas reinhardtii, tagged with Venus at the C-terminus under CMV promoter.
RDB16771 pCMV-chopFR-Venus Mammalian expression vector encoding a chimeric channelopsin (channelopsin/fast-receiver, chopFR) between chop1 and chop2 from Chlamydomonas reinhardtii, tagged with Venus at the C-terminus under CMV promoter.
RDB16772 pCMV-chopWR-Venus Mammalian expression vector encoding a chimeric channelopsin (channelopsin/wide-receiver, chopWR) between chop1 and chop2 from Chlamydomonas reinhardtii, tagged with Venus at the C-terminus under CMV promoter.
RDB16773 pCMV-ChRFR-Venus Mammalian expression vector encoding a mammalian codon-optimized chopFR tagged with Venus at the C-terminus under CMV promoter.
RDB16774 pCMV-ChRWR-Venus Mammalian expression vector encoding a mammalian codon-optimized chopWR tagged with Venus at the C-terminus under CMV promoter.

(GRP0057e 2019.01.10 T.M.)

2023.06.08



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