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Genetic Resources for Optgenetics Research


Organelle Optogenetics: Direct Manipulation of Intracellular Ca2+ Dynamics by Light.

Asano, T., Igarashi, H., Ishizuka, T., Yawo, H.
Front. Neurosci. 12: 561 (2018). PubMed PMID 30174581.

Catalog No. Name of resource description
RDB16748 pCAGGS-ChRGR-Venus Expression vector of organelle optogenetics channelrhodopsin.
RDB16750 pCAGGS-ChRGR(ER)-Venus Expression vector of organelle optogenetics channelrhodopsin.

Expression vector of the channelrhodopsin-green receiver (ChRGR) (cat# RDB16748) which induces membrane potential oscillation by green light irradiation.
Expression vector of the ChRGR(ER) (cat# RDB16750) which localizes ChRGR to endoplasmic/sarcoplasmic reticulum (ER/SR). ChRGR(ER) is used as a tool to manipulation of intracellular calcium ion release.
Depositor: Dr. Toru Ishizuka.


A Chimera Na+-Pump Rhodopsin as an Effective Optogenetic Silencer.

Hoque, M.R., Ishizuka, T., Inoue, K., Abe-Yoshizumi, R., Igarashi, H., Mishima, T., Kandori, H., Yawo, H.
PLoS One 11 (11): e0166820 (2016). PubMed PMID 27861619.

Catalog No. Name of resource description
RDB16777 pCMV-KR2-3.0-EYFP Mammalian expression vector encoding a light-driven sodiun ion pump from Krokinobacter eikastus (KR2) fused with a membrane targeting signal (TS), eYFP, and an ER export signal (ERex) at the C-terminus under CMV promoter.
RDB16778 pCaMKIIα-KR2-3.0-EYFP-WPRE Mammalian expression vector encoding a light-driven sodiun ion pump from Krokinobacter eikastus (KR2) fused with a membrane targeting signal (TS), eYFP, and an ER export signal (ERex) at the C-terminus under CaMKIIα promoter.
RDB16779 pCMV-IaNaR-3.0-EYFP Mammalian expression vector encoding a light-driven sodiun ion pump from Indibacter alkaliphilus (IaNaR) fused with a membrane targeting signal (TS), eYFP, and an ER export signal (ERex) at the C-terminus under CMV promoter.
RDB16780 pCMV-I1K6NaR-3.0-EYFP Mammalian expression vector encoding a chimeric NaR between IaNaR and KR2 fused with a membrane targeting signal (TS), eYFP, and ER export signal (ERex) at the C-terminus under CMV promoter.
RDB16781 pCMV-I2K5NaR-3.0-EYFP Mammalian expression vector encoding a chimeric NaR between IaNaR and KR2 fused with a membrane targeting signal (TS), eYFP, and ER export signal (ERex) at the C-terminus under CMV promoter.
RDB16782 pCMV-I3K4NaR-3.0-EYFP Mammalian expression vector encoding a chimeric NaR between IaNaR and KR2 fused with a membrane targeting signal (TS), eYFP, and ER export signal (ERex) at the C-terminus under CMV promoter.
RDB16783 pCMV-I4K3NaR-3.0-EYFP Mammalian expression vector encoding a chimeric NaR between IaNaR and KR2 fused with a membrane targeting signal (TS), eYFP, and ER export signal (ERex) at the C-terminus under CMV promoter.
RDB16784 pCMV-I5K2NaR-3.0-EYFP Mammalian expression vector encoding a chimeric NaR between IaNaR and KR2 fused with a membrane targeting signal (TS), eYFP, and ER export signal (ERex) at the C-terminus under CMV promoter.
RDB16785 pCMV-I6K1NaR-3.0-EYFP Mammalian expression vector encoding a chimeric NaR between IaNaR and KR2 fused with a membrane targeting signal (TS), eYFP, and ER export signal (ERex) at the C-terminus under CMV promoter.

Depositor: Dr. Toru Ishizuka.


The regulatory mechanism of ion permeation through a channelrhodopsin derived from Mesostigma viride (MvChR1).

Watanabe, S., Ishizuka, T., Hososhima, S., Zamani, A., Hoque, M.R., Yawo, H.
Photochem. Photobiol. Sci. 15 (3): 365-374 (2016). PubMed PMID 26853505.

Catalog No. Name of resource description
RDB16786 pCMV-MvChR1-Venus Mammalian expression vector encoding Met1-Arg323 of channelrhodopsin 1 from Mesostigma viride (MvChR1) tagged with Venus at the C-terminus under CMV promoter.
RDB16787 pCMV-eMvChR1#1-Venus Mammalian expression vector encoding an N-terminal variant of MvChR1 (eMvChR1#1) tagged with Venus at the C-terminus under CMV promoter.
RDB16788 pCMV-eMvChR1#2-Venus Mammalian expression vector encoding an N-terminal variant of MvChR1 (eMvChR1#2) tagged with Venus at the C-terminus under CMV promoter.
RDB16789 pCMV-eMvChR1#2(A97D)-Venus Mammalian expression vector encoding an N-terminal variant of MvChR1 (eMvChR1#2) tagged with Venus at the C-terminus under CMV promoter. A97D mutation was introduced.
RDB16790 pCMV-eMvChR1#2(A97E)-Venus Mammalian expression vector encoding an N-terminal variant of MvChR1 (eMvChR1#2) tagged with Venus at the C-terminus under CMV promoter. A97E mutation was introduced.
RDB16791 pCMV-eMvChR1#2(A97Q)-Venus Mammalian expression vector encoding an N-terminal variant of MvChR1 (eMvChR1#2) tagged with Venus at the C-terminus under CMV promoter. A97Q mutation was introduced.
RDB16792 pCMV-eMvChR1#2(A97R)-Venus Mammalian expression vector encoding an N-terminal variant of MvChR1 (eMvChR1#2) tagged with Venus at the C-terminus under CMV promoter. A97R mutation was introduced.

Depositor: Dr. Toru Ishizuka.


Near-infrared (NIR) up-conversion optogenetics.

Hososhima, S., Yuasa, H., Ishizuka, T., Hoque, M.R., Yamashita, T., Yamanaka, A., Sugano, E., Tomita, H., Yawo, H.
Sci. Rep. 5: 16533 (2015). PubMed PMID 26552717.

Catalog No. Name of resource description
RDB16793 pCMV-C1V1-Venus Mammalian expression vector encoding a chimeric channelrhodopsin (C1V1) tagged with Venus at the C-terminus under CMV promoter.
RDB16794 pCMV-PsChR-Venus Mammalian expression vector encoding a channelrhodopsin from Platymonas (Tetraselmis) subcordiformis (PsChR) tagged with Venus at the C-terminus under CMV promoter.
RDB16795 pCaMKIIα-C1V1-Venus Mammalian expression vector encoding a chimeric channelrhodopsin (C1V1) tagged with Venus at the C-terminus under CaMKIIa promoter.
RDB16796 pCaMKIIα-PsChR-Venus Mammalian expression vector encoding a channelrhodopsin from Platymonas (Tetraselmis) subcordiformis (PsChR) tagged with Venus at the C-terminus under CaMKIIa promoter.

Depositor: Dr. Toru Ishizuka.


Structural basis for Na(+) transport mechanism by a light-driven Na(+) pump.

Kato, H.E., Inoue, K., Abe-Yoshizumi, R., Kato, Y., Ono, H., Konno, M., Hososhima, S., Ishizuka, T., Hoque, M.R., Kunitomo, H., Ito, J., Yoshizawa, S., Yamashita, K., Takemoto, M., Nishizawa, T., Taniguchi, R., Kogure, K., Maturana, A.D., Iino, Y., Yawo, H., Ishitani, R., Kandori, H., Nureki, O.
Nature 521 (7550): 48-53 (2015). PubMed PMID 25849775.

Catalog No. Name of resource description
RDB16777 pCMV-KR2-3.0-EYFP Mammalian expression vector encoding a light-driven sodiun ion pump from Krokinobacter eikastus (KR2) fused with a membrane targeting signal (TS), eYFP, and an ER export signal (ERex) at the C-terminus under CMV promoter.
RDB16778 pCaMKIIα-KR2-3.0-EYFP-WPRE Mammalian expression vector encoding a light-driven sodiun ion pump from Krokinobacter eikastus (KR2) fused with a membrane targeting signal (TS), eYFP, and an ER export signal (ERex) at the C-terminus under CaMKIIa promoter.

Depositor: Dr. Toru Ishizuka.


Opto-current-clamp actuation of cortical neurons using a strategically designed channelrhodopsin.

Wen, L., Wang, H., Tanimoto, S., Egawa, R., Matsuzaka, Y., Mushiake, H., Ishizuka, T., Yawo, H.
PLoS One 5 (9): e12893 (2010). PubMed PMID 20886118.

Catalog No. Name of resource description
RDB16775 pCMV-chopGR-Venus Mammalian expression vector encoding a chimeric channelopsin (channelopsin/green-receiver, chopGR) between chop1 and chop2 from Chlamydomonas reinhardtii, tagged with Venus at the C-terminus under CMV promoter.
RDB16776 pCMV-ChRGR-Venus Mammalian expression vector encoding a mammalian codon-optimized chopGR tagged with Venus at the C-terminus under CMV promoter.

Depositor: Dr. Toru Ishizuka.


Molecular determinants differentiating photocurrent properties of two channelrhodopsins from Chlamydomonas.

Wang, H., Sugiyama, Y., Hikima, T., Sugano, E., Tomita, H., Takahashi, T., Ishizuka, T., Yawo, H.
J. Biol. Chem. 284 (9): 5685-5696 (2009). PubMed PMID 19103605.

Catalog No. Name of resource description
RDB16769 pCMV-chop1-Venus Mammalian expression vector encoding channelopsin-1 (ChR1) from Chlamydomonas reinhardtii, tagged with Venus at the C-terminus under CMV promoter.
RDB16770 pCMV-chop2-Venus Mammalian expression vector encoding channelopsin-2 (ChR2) from Chlamydomonas reinhardtii, tagged with Venus at the C-terminus under CMV promoter.
RDB16771 pCMV-chopFR-Venus Mammalian expression vector encoding a chimeric channelopsin (channelopsin/fast-receiver, chopFR) between chop1 and chop2 from Chlamydomonas reinhardtii, tagged with Venus at the C-terminus under CMV promoter.
RDB16772 pCMV-chopWR-Venus Mammalian expression vector encoding a chimeric channelopsin (channelopsin/wide-receiver, chopWR) between chop1 and chop2 from Chlamydomonas reinhardtii, tagged with Venus at the C-terminus under CMV promoter.
RDB16773 pCMV-ChRFR-Venus Mammalian expression vector encoding a mammalian codon-optimized chopFR tagged with Venus at the C-terminus under CMV promoter.
RDB16774 pCMV-ChRWR-Venus Mammalian expression vector encoding a mammalian codon-optimized chopWR tagged with Venus at the C-terminus under CMV promoter.

Depositor: Dr. Toru Ishizuka.

(GRP0057e 2019.01.10 T.M.)

2019.01.15