Gene Engineering Division - DNA BANK - > Research tools > Fucci

Fucci

FacebookXBluesky

Fucci – Fluorescent Ubiquitination-based Cell Cycle Indicator

Fucci5

Ando, R., et al. Cell Struct. Funct. 48 (2): 135-144 (2023). PMID 37394513.

DNA materials

Cat. no. Resource name Description
RDB18220 tFucci(SA)5/pCS2 CMV promoter, hCdt1, AzaleaB5, hGeminin, h2-3
RDB18221 tFucci(CA)5/pCS2 CMV promoter, hCdt1, AzaleaB5, hGeminin, h2-3

Cell line

Cat. no. Resource name
RCB4917 HeLa/Fucci(SA)5#16
RCB4919 HeLa/Fucci(CA)5#16

Cell cycle indicator Fucci(CA) and Fucci(SA)

Sakaue-Sawano, A. et al., Mol. Cell 68 (3): 626-640.e5 (2017). PMID 29107535.

DNA materials

Cat. no. Resource name Description
RDB15459 mCherry-hCdt1(1/100)Cy(-)/pcDNA3 CMV promoter, hCdt1, mCherry
RDB15273 mCherry-hCdt1(30/120) / pCSII-EF EF-1α promoter, hCtd1, mCherry, lentivirus vector
RDB15441 mCherry-hCdt1(1/100)Cy(-)/pCSII-EF EF-1α promoter, hCtd1, mCherry, lentivirus vector
RDB15442 mCherry-hCdt1(1/100)/pCSII-EF EF-1α promoter, hCtd1, mCherry, lentivirus vector
RDB15271 mVenus-hGeminin(1/110) / pCSII-EF EF-1α promoter, hGeminin, mVenus, lentivirus vector
RDB15446 tFucci(CA)2/pCSII-EF EF-1α promoter, hCtd1, mCherry, hGeminin, mVenus, lentivirus vector
RDB15452 tFucci(CA)2/pCSII-CMV CMV promoter, hCtd1, mCherry, hGeminin, mVenus, lentivirus vector
RDB15447 tFucci(CA)2.1/pCSII-EF EF-1α promoter, hCtd1, mCherry, hGeminin, AmCyan, lentivirus vector
RDB15453 tFucci(CA)2.1/pCSII-CMV CMV promoter, hCtd1, mCherry, hGeminin, AmCyan, lentivirus vector
RDB15448 tFucci(CA)2.2/pCSII-EF EF-1α promoter, hCtd1, mCherry, hGeminin, mTurquoise, lentivirus vector
RDB15454 tFucci(CA)2.2/pCSII-CMV CMV promoter, hCtd1, mCherry, hGeminin, mTurquoise, lentivirus vector
RDB15443 tFucci(SA)2/pCSII-EF EF-1α promoter, hCtd1, mCherry, hGeminin, mVenus, lentivirus vector
RDB15449 tFucci(SA)2/pCSII-CMV CMV promoter, hCtd1, mCherry, hGeminin, mVenus, lentivirus vector
RDB15444 tFucci(SA)2.1/pCSII-EF EF-1α promoter, hCtd1, mCherry, hGeminin, AmCyan, lentivirus vector
RDB15450 tFucci(SA)2.1/pCSII-CMV CMV promoter, hCtd1, mCherry, hGeminin, AmCyan, lentivirus vector
RDB15445 tFucci(SA)2.2/pCSII-EF EF-1α promoter, hCtd1, mCherry, hGeminin, mTurquoise, lentivirus vector
RDB15451 tFucci(SA)2.2/pCSII-CMV CMV promoter, hCtd1, mCherry, hGeminin, mTurquoise, lentivirus vector

Cell line

Cat. no. Resource name
RCB4676 HeLa/Fucci(CA)2
RCB4675 NMuMG/Fucci(CA)2.1

Cell cycle indicator Fucci2a

Ford, M.J. et al., Dev. Cell 47 (4): 509-523.e5 (2018). PMID 30458140.
Mort, R.L. et al., Cell Cycle 13 (17): 2681-2696 (2014). PMID 25486356.

DNA materials

Cat. no. Resource name Description
RDB16057 pCDNA5-Frt-CAG-Arl13bCerulean-Fucci2a CAG promoter, Arl13b, Cerulean, hGem(1/110), mVenus, hCdt1(30/120), mCherry
RDB16058 pRosa26-CAG-floxNeo-Arl13bCerulean-Fucci2a >CAG promoter, Arl13b, Cerulean, hGem(1/110), mVenus, hCdt1(30/120), mCherry
RDB16059 pCAG-Arl13bCerulean-Fucci2a >CAG promoter, Arl13b, Cerulean, hGem(1/110), mVenus, hCdt1(30/120), mCherry
RDB13080 pCAG-Fucci2a CAG promoter, hGem(1/110), mVenus, hCdt1(30/120), mCherry
RDB13081 pROSA-floxNeo-Fucci2a CAG promoter, hGem(1/110), mVenus, hCdt1(30/120), mCherry
RDB16056 pCDNA5-Frt-Fucci2a CMV promoter, hGem(1/110), mVenus, hCdt1(30/120), mCherry

Mouse

Cat. no. Resource name
RBRC 06511 B6;129-Gt(ROSA)26Sor<tm1(Fucci2aR)Jkn>

Cell line

Cat. no. Resource name
AES0223 MESC Fucci2aR
AES0224 MESC Fucci2a
RCB5029 NIH3T3 Flp Arl13bCerulean-Fucci2a
RCB5030 NIH3T3 Flp Fucci2a
AES0225 MESC Arl13b-Fucci2aR
AES0226 MESC Arl13b-Fucci2a

Cell cycle indicator Fucci2

Sakaue-Sawano, A. et al., BMC Cell Biol. 12: 2 (2011). PMID 21226962.
Sakaue-Sawano, A. et al., Chem. Biol. 15 (12): 1243-1248 (2008). PMID 9101468.

DNA materials

Cat. no. Resource name Description
RDB15457 mCherry-hCdt1(30/120)/pcDNA3 CMV promoter, hCdt1, mCherry
RDB15458 mVenus-hGeminin(1/110)/pcDNA3 CMV promoter, hGeminin, mVenus
RDB15273 mCherry-hCdt1(30/120) / pCSII-EF EF-1α promoter, hCtd1, mCherry, lentivirus vector
RDB15271 mVenus-hGeminin(1/110) / pCSII-EF EF-1α promoter, hGeminin, mVenus, lentivirus vector
RDB15274 AmCyan-hGem(1/110) / pCSII-EF EF-1α promoter, hGeminin, AmCyan, lentivirus vector

Cell line

Cat. no. Resource name
RCB2867 HeLa/Fucci2
RCB2868 NMuMG/Fucci2

Cell cycle indicator Fucci

Sakaue-Sawano, A. et al., Development 140 (22): 4624-4632 (2013). PMID 24154524.
Aiba, Y, et al., Proc. Natl. Acad. Sci. USA. 107 (27): 12192-12197 (2010). PMID 20547847.
Sakaue-Sawano, A., Cell 132 (3): 487-498 (2008). PMID 18267078.

DNA materials

Cat. no. Resource name Description
RDB15267 mKO2-hCdt1(30/120) / pCSII-EF EF-1α promoter, hCtd1, mKO2, lentivirus vector
RDB15268 mAG-hGeminin(1/110) / pCSII-EF EF-1α promoter, hGeminin, mAG, lentivirus vector

Mouse

Cat. no. Resource name
RBRC02706 B6.Cg-Tg (FucciS/G2/M) #504Bsi
RBRC02707 B6.Cg-Tg (FucciG1) #596Bsi
RBRC02704 B6.Cg-Tg(FucciS/G2/M)#474Bsi
RBRC02708 B6.Cg-Tg(FucciG1)#610Bsi
RBRC02705 B6.Cg-Tg(FucciS/G2/M)#492Bsi
RBRC02709 B6.Cg-Tg(FucciG1)#639Bsi

Cell line

Cat. no. Resource name
RCB2812 HeLa/Fucci
RCB2813 NMuMG/Fucci
RCB2814 COS/Fucci

Cell cycle indicator

Sakaue-Sawano, A. et al., Chem. Biol. 15 (12): 1243-1248 (2008). PMID 19101468.

DNA materials

Cat. no. Resource name Description
RDB15269 mCherry-hGeminin(1/110) / pCSII-EF EF-1α promoter, hGeminin, mCherry, lentivirus vector
RDB15270 mCherry-hGeminin(1/60) / pCSII-EF EF-1α promoter, hGeminin, mCherry, lentivirus vector
RDB15272 mVenus-hGeminin(1/60) / pCSII-EF EF-1α promoter, hGeminin, mVenus, lentivirus vector
RDB15274 AmCyan-hGem(1/110)/pCSII-EF EF-1α promoter, hGeminin, AmCyan, lentivirus vector

Tol2 plasmid

Sugiyama, M. et al., Proc. Natl. Acad. Sci. USA. 106 (49): 20812-20817 (2009). PMID 19923430.

DNA materials

Cat. no. Resource name
RDB15278 mCherry-zCdt1(1/190) / pT2KXIGdeltain
RDB15279 mVenus-zGeminin (1/100) / pT2KXIGdeltain
RDB15275 mKO2-zCdt1(1/190) / pT2KXIGdeltain
RDB15276 mAG-zGeminin(1/100) / pT2KXIGdeltain
RDB15277 mAG-hGeminin (1/60) / pT2KXIGdeltain

Other Fucci related materials

RIKEN Center for Brain Science (CBS), Dr. Miyawaki lab.

References

Important notice regarding nomenclature of Fucci

Mouse of the Month

(GRP0044e 2017.09.18 T.M.)

2024.09.13



 

Comments are closed.