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Takara Bio USA, Inc. and RIKEN BioResource Research Center concluded license agreement on preservation and distribution of Fluorescent Proteins, such as DsRed2 and mCherry for academic use

BRC News

fucci


Fucci – Fluorescent Ubiquitination-based Cell Cycle Indicator

Fucci related materials
B6.Cg-Tg (FucciS/G2/M) #504Bsi (RBRC02706)
B6.Cg-Tg (FucciG1) #596Bsi (RBRC02707)
Sakaue-Sawano, A. et al., 2008a
B6.Cg-Tg(FucciS/G2/M)#492Bsi (RBRC02705)
B6.Cg-Tg(FucciG1)#639Bsi (RBRC02709)
Aiba, Y, et al., 2010
B6.Cg-Tg(FucciS/G2/M)#474Bsi (RBRC02704)
B6.Cg-Tg(FucciG1)#610Bsi (RBRC02708)
Sakaue-Sawano, A. et al., 2013.
B6;129-Gt(ROSA)26Sor<tm1(Fucci2aR)Jkn> (RBRC 06511) Mort, R.L. et al., 2014
Search Fucci mouse strains
HeLa/Fucci (RCB2812)
NMuMG/Fucci (RCB2813)
COS/Fucci (RCB2814)
Sakaue-Sawano, A. et al., 2008a
HeLa/Fucci2 (RCB2867)
NMuMG/Fucci2 (RCB2868)
Sakaue-Sawano, A. et al., 2011
HeLa/Fucci(CA)2 (RCB4676) Sakaue-Sawano, A. et al., 2017
Search Fucci cells
DNA materials
(RIKEN BRC
DNA Bank
)
Tol2 plasmid
mCherry-zCdt1(1/190) / pT2KXIGdeltain (RDB15278)
mVenus-zGeminin (1/100) / pT2KXIGdeltain (RDB15279)
mKO2-zCdt1(1/190) / pT2KXIGdeltain (RDB15275)
mAG-zGeminin(1/100) / pT2KXIGdeltain (RDB15276)
mAG-hGeminin (1/60) / pT2KXIGdeltain (RDB15277)
Sugiyama, M. et al., 2009
mCherry-hCdt1(30/120)/pcDNA3 (RDB15457)
mVenus-hGeminin(1/110)/pcDNA3 (RDB15458)
Sakaue-Sawano, A. et al., 2011
pCAG-Fucci2a (RDB13080)
pROSA-floxNeo-Fucci2a (RDB13081)
pCDNA5-Frt-Fucci2a (RDB16056)
Mort, R.L. et al., 2014
mCherry-hCdt1(1/100)Cy(-)/pcDNA3 (RDB15459) Sakaue-Sawano, A. et al., 2017
pCDNA5-Frt-CAG-Arl13bCerulean-Fucci2a (RDB16057)
pRosa26-CAG-floxNeo-Arl13bCerulean-Fucci2a (RDB16058)
pCAG-Arl13bCerulean-Fucci2a (RDB16059)
Ford, M.J. et al., 2018
Lentiviral vector
(RIKEN BRC)
mCherry-hGeminin(1/110) / pCSII-EF
mCherry-hGeminin(1/60) / pCSII-EF
mVenus-hGeminin(1/60) / pCSII-EF
Sakaue-Sawano, A. et al., 2008b
Fucci2
mCherry-hCdt1(30/120) / pCSII-EF
mVenus-hGeminin(1/110) / pCSII-EF
Sakaue-Sawano, A. et al., 2008b
Sakaue-Sawano, A. et al., 2011
tFucci(CA) and tFucci(SA)
mCherry-hCdt1(1/100)Cy(-)/pCSII-EF
mCherry-hCdt1(1/100)/pCSII-EF
Sakaue-Sawano, A. et al., 2017
Other Fucci related materials
(RIKEN Center for Brain Science (CBS), Dr. Miyawaki lab.)

References

Important notice regarding nomenclature of Fucci

Original papers

  • Aiba, Y, et al. (2010) Preferential localization of IgG memory B cells adjacent to contracted germinal centers. Proc. Natl. Acad. Sci. USA. 107 (27): 12192-12197. PMID: 20547847.
  • Ford, M.J. et al. (2018) A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice. Dev. Cell 47 (4): 509-523.e5. PMID: 30458140.
  • Mort, R.L. et al. (2014) Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice. Cell Cycle 13 (17): 2681-2696. PMID: 25486356.
  • Sakaue-Sawano, A. et al. (2008a) Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498. PMID: 18267078.
  • Sakaue-Sawano, A. et al. (2008b) Tracing the silhouette of individual cells in S/G2/M phases with fluorescence. Chem. Biol. 15 (12): 1243-1248. PMID: 19101468.
  • Sakaue-Sawano, A. et al. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2. PMID: 21226962.
  • Sakaue-Sawano, A. et al. (2013) Visualizing developmentally programmed endoreplication in mammals using ubiquitin oscillators. Development 140 (22): 4624-4632. PMID: 24154524.
  • Sakaue-Sawano, A. et al. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5. PMID: 29107535.
  • Sugiyama, M. et al. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. USA. 106 (49): 20812-20817. PMID: 19923430.

Mouse of the Month

(GRP0044e 2017.09.18 T.M.)

2020.01.31