Clone Search

Resources for

Clones & Vectors for Gene Expression
  in E. coli
  in T. thermophilus HB8
  in S. pombe
  in S. cerevisiae
  in Mammalian Cells

Research Tools
  Genome Editing
  Fluorescent Proteins
  Luminescent Proteins
  Sensor & Visualization
  Plant gene resources

Clone Set, Library & Genomic DNA
  Genomic clone
  cDNA clone
  Expression clone
  Genomic DNA

Recombinant Virus
  Recombinant Adenovirus
  Shuttle Vectors

Gene Set Collection
  Circadian Clock
  Notch Signaling
  Sphingolipid Signaling

Search and Browse
  Key word search
  Browsing by category
  Browsing by species
  Depositors List

Takara Bio USA, Inc. and RIKEN BioResource Research Center concluded license agreement on preservation and distribution of Fluorescent Proteins, such as DsRed2 and mCherry for academic use

BRC News


Fucci – Fluorescent Ubiquitination-based Cell Cycle Indicator

Fucci related materials
B6.Cg-Tg (FucciS/G2/M) #504Bsi (RBRC02706)
B6.Cg-Tg (FucciG1) #596Bsi (RBRC02707)
Sakaue-Sawano, A. et al., 2008a
B6.Cg-Tg(FucciS/G2/M)#492Bsi (RBRC02705)
B6.Cg-Tg(FucciG1)#639Bsi (RBRC02709)
Aiba, Y, et al., 2010
B6.Cg-Tg(FucciS/G2/M)#474Bsi (RBRC02704)
B6.Cg-Tg(FucciG1)#610Bsi (RBRC02708)
Sakaue-Sawano, A. et al., 2013.
B6;129-Gt(ROSA)26Sor<tm1(Fucci2aR)Jkn> (RBRC 06511) Mort, R.L. et al., 2014
Search Fucci mouse strains
HeLa/Fucci (RCB2812)
NMuMG/Fucci (RCB2813)
COS/Fucci (RCB2814)
Sakaue-Sawano, A. et al., 2008a
HeLa/Fucci2 (RCB2867)
NMuMG/Fucci2 (RCB2868)
Sakaue-Sawano, A. et al., 2011
HeLa/Fucci(CA)2 (RCB4676) Sakaue-Sawano, A. et al., 2017
Search Fucci cells
DNA materials
DNA Bank
Tol2 plasmid
mCherry-zCdt1(1/190) / pT2KXIGdeltain (RDB15278)
mVenus-zGeminin (1/100) / pT2KXIGdeltain (RDB15279)
mKO2-zCdt1(1/190) / pT2KXIGdeltain (RDB15275)
mAG-zGeminin(1/100) / pT2KXIGdeltain (RDB15276)
mAG-hGeminin (1/60) / pT2KXIGdeltain (RDB15277)
Sugiyama, M. et al., 2009
mCherry-hCdt1(30/120)/pcDNA3 (RDB15457)
mVenus-hGeminin(1/110)/pcDNA3 (RDB15458)
Sakaue-Sawano, A. et al., 2011
pCAG-Fucci2a (RDB13080)
pROSA-floxNeo-Fucci2a (RDB13081)
pCDNA5-Frt-Fucci2a (RDB16056)
Mort, R.L. et al., 2014
mCherry-hCdt1(1/100)Cy(-)/pcDNA3 (RDB15459) Sakaue-Sawano, A. et al., 2017
pCDNA5-Frt-CAG-Arl13bCerulean-Fucci2a (RDB16057)
pRosa26-CAG-floxNeo-Arl13bCerulean-Fucci2a (RDB16058)
pCAG-Arl13bCerulean-Fucci2a (RDB16059)
Ford, M.J. et al., 2018
Lentiviral vector
mCherry-hGeminin(1/110) / pCSII-EF
mCherry-hGeminin(1/60) / pCSII-EF
mVenus-hGeminin(1/60) / pCSII-EF
Sakaue-Sawano, A. et al., 2008b
mCherry-hCdt1(30/120) / pCSII-EF
mVenus-hGeminin(1/110) / pCSII-EF
Sakaue-Sawano, A. et al., 2008b
Sakaue-Sawano, A. et al., 2011
tFucci(CA) and tFucci(SA)
Sakaue-Sawano, A. et al., 2017
Other Fucci related materials
(RIKEN Center for Brain Science (CBS), Dr. Miyawaki lab.)


Important notice regarding nomenclature of Fucci

Original papers

  • Aiba, Y, et al. (2010) Preferential localization of IgG memory B cells adjacent to contracted germinal centers. Proc. Natl. Acad. Sci. USA. 107 (27): 12192-12197. PMID: 20547847.
  • Ford, M.J. et al. (2018) A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice. Dev. Cell 47 (4): 509-523.e5. PMID: 30458140.
  • Mort, R.L. et al. (2014) Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice. Cell Cycle 13 (17): 2681-2696. PMID: 25486356.
  • Sakaue-Sawano, A. et al. (2008a) Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498. PMID: 18267078.
  • Sakaue-Sawano, A. et al. (2008b) Tracing the silhouette of individual cells in S/G2/M phases with fluorescence. Chem. Biol. 15 (12): 1243-1248. PMID: 19101468.
  • Sakaue-Sawano, A. et al. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2. PMID: 21226962.
  • Sakaue-Sawano, A. et al. (2013) Visualizing developmentally programmed endoreplication in mammals using ubiquitin oscillators. Development 140 (22): 4624-4632. PMID: 24154524.
  • Sakaue-Sawano, A. et al. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5. PMID: 29107535.
  • Sugiyama, M. et al. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. USA. 106 (49): 20812-20817. PMID: 19923430.

Mouse of the Month

(GRP0044e 2017.09.18 T.M.)