Gene Engineering Division > Research tools > Fluorescent protein resources

Fluorescent protein resources

Fluorescent protein resource

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Cyan | Green | Yellow | Orange | Red
 
photoconversion type | photochromism type | Destabilized- and Secreted-FPs | Non-fluorescent chromoprotein
 
Tools | Thermostable FP | Sensor & Visualization | Luminescent protein

Cyan

Kusabira Cyan

Emitting bright cyan light. There is less overlap of fluorescence spectra on yellow than ECFP.

Kusabira-Cyan CoralHue™ Fluorescent Protein
Kikuchi A et al. Crystal structure of a new cyan fluorescent protein and its hue-shifted variants. Biochemistry 48, 5276-83 (2009) (PMID: 19402703)
Abbr. Form Excitation maxima (nm) Emission maxima (nm) S1 vector# MC1 vector$ MN1 vector$ MCLinker vector* MNLinker vector*
KCy1 Dimer 453 486 RDB18310        

Midoriishi Cyan

Emitting cyan light. There is less overlap of fluorescence spectra on yellow than ECFP

Midoriishi-Cyan CoralHue™ Fluorescent Protein
Karasawa S et al. Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem J. 381, 307-312 (2004) (PMID: 15065984)
Abbr. Form Excitation maxima (nm) Emission maxima (nm) S1 vector# MC1 vector$ MN1 vector$ MCLinker vector* MNLinker vector*
MiCy1 Dimer 472 495 RDB18265        
mMiCy1 Monomer 470 496 RDB18289        
hmMiCy1 Monomer 470 496   RDB18290 RDB18291 RDB18292 RDB18288
Protein Resource (cat.num.) Reference
Midoriishi-Cyan mMiCy1/pRSETB (RDB15241) Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer.
Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312 (2004). PubMed PMID 15065984.

CFP

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Green

Umikinoko Green

Emitting brilliant green light. It has high pH stability.

Umikinoko-Green CoralHue™ Fluorescent Protein
Tsutsui H et al. Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat Methods. 5, 683-685 (2008) (PMID: 18622396)
Abbr. Form Excitation maxima (nm) Emission maxima (nm) S1 vector# MC1 vector$ MN1 vector$ MCLinker vector* MNLinker vector*
mUKG1 Monomer 483 499 RDB18306        
hmUKG1 Monomer 483 499 RDB18307 RDB18308 RDB18309    

Azami Green

Emitting green light.

Azami-Green CoralHue™ Fluorescent Protein
Karasawa S et al. A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J Biol Chem. 278, 34167-34171 (2003) (PMID: 12819206)
Abbr. Form Excitation maxima (nm) Emission maxima (nm) S1 vector# MC1 vector$ MN1 vector$ MCLinker vector* MNLinker vector*
AG Tetramer 492 505 RDB18244        
mAG1 Monomer 492 505 RDB18246 RDB18247 RDB18248    
hmAG1 Monomer 492 505 RDB18249 RDB18250 RDB18251 RDB18252 RDB18245
hmAG407 Monomer 407 498 RDB18328        
Protein Resource (cat.num.) Reference
Azami Green hmAG407/pRSETB (RDB15229)
hmAG1/pRSETB (RDB15230). 		A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling.
Karasawa, S. et al., J. Biol. Chem. 278 (36): 34167-34171 (2003). PubMed PMID 12819206.

h2-3

Protein Resource (cat.num.) Reference
h2-3 h2-3/pRSETb (RDB18219) Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression.
Ando, R. et al., bioRxiv (2020) doi: https://doi.org/10.1101/2020.03.30.015156.

GFP

Protein Resource (cat.num.) Reference
GFPS1 pGFPS1 (RDB09057)
pUC-GFPS1 (RDB09059)		 Cell-free protein synthesis system from Escherichia coli cells cultured at decreased temperatures improves productivity by decreasing DNA template degradation.
Seki, E. et al., Anal. Biochem. 377 (2): 156-161. PMID 18375196.

StayGold

Protein Resource (cat.num.) Reference
StayGold (n1)StayGold/pRSET (RDB19605)
(n1)StayGold(c4)/pRSET (RDB19606)
(n1)oxStayGold/pRSET (RDB19607)
(n1)oxStayGold(c4)/pRSET (RDB19608)
tdStayGold/pcDNA3 (RDB19609)
tdStayGold(c4)/pBS Coupler (RDB19610)
tdoxStayGold/pcDNA3 (RDB19611)
tdoxStayGold(c4)/pBS Coupler (RDB19612)		 A highly photostable and bright green fluorescent protein.
Hirano, M. et al., Nat. Biotechnol. 2022 Apr 25. doi: 10.1038/s41587-022-01278-2. Epub ahead of print. PMID 35468954.

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Yellow

Achilles

Protein Resource (cat.num.) Reference
Achilles Achilles/pRSETB (RDB15982) Coupling delay controls synchronized oscillation in the segmentation clock.
Yoshioka-Kobayashi, K. et al., Nature. 2020 Jan 8. doi: 10.1038/s41586-019-1882-z. [Epub ahead of print]. PMID 31915376.

Venus

Protein Resource (cat.num.) Reference
Venus Venus/pCS2 (RDB15116)
mVenus/pRSETB (RDB15117) 		A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications.
Nagai, T. et al., Nat. Biotechnol. 20 (1): 87-90 (2002). PubMed PMID 11753368.
Venus cp49Venus (RDB15120)
cp145Venus (RDB15121)
cp157Venus (RDB15122)
cp173Venus (RDB15123)
cp195Venus (RDB15124)
cp229Venus (RDB15125) 		Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins.
Nagai, T. et al., Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PubMed PMID 15247428.

Cy11.5

Protein Resource (cat.num.) Reference
Cy11.5 Cy11.5/pRSETB (RDB15706) Concatenation of cyan and yellow fluorescent proteins for efficient resonance energy transfer.
Shimozono, S. et al., Biochemistry 45 (20): 6267-6271 (2006). PubMed PMID 16700538.

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Orange

Kusabira Orange

Emitting orange light. It has pH stability. Its sharp fluorescence spectrum enables multicolor fluorescence imaging with red fluorescent proteins which are difficult to use other orange fluorescent protein.

Kusabira-Orange CoralHue™ Fluorescent Protein
Karasawa S et al. Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochem J. 381, 307-312 (2004) (PMID: 15065984)
Abbr. Form Excitation maxima (nm) Emission maxima (nm) S1 vector# MC1 vector$ MN1 vector$ MCLinker vector* MNLinker vector*
KO1 Dimer 548 561 RDB18253        
mKO1 Monomer 548 559 RDB18258 RDB18259 RDB18260    
mKO2 Monomer 551 565 RDB18299        
hKO1 Dimer 548 561 RDB18254 RDB18255 RDB18256    
hmKO1 Monomer 548 559 RDB18261 RDB18262 RDB18263 RDB18264 RDB18257
hmKO2 Monomer 551 565   RDB18300 RDB18301 RDB18302 RDB18298
Protein Resource (cat.num.) Reference
Kusabira Orange hmKO1/pRSETB (RDB15312)
hmKO2/pRSETB (RDB15313) 		Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer.
Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312 (2004). PubMed PMID 15065984.
Kusabira Orange hmKO-K/pRSETB (RDB15314) Improving membrane voltage measurements using FRET with new fluorescent proteins.
Tsutsui, H. et al., Nat. Methods 5 (8): 683-685 (2008). PubMed PMID 18622396.

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Red

AzaleaB5

Protein Resource (cat.num.) Reference
AzaleaB5 AzaleaB5/pRSETb (RDB18218) Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression.
Ando, R. et al., bioRxiv (2020) doi: https://doi.org/10.1101/2020.03.30.015156.

Keima570 and Keima-Red

Emitting orange light (maximum fluorescence 570 nm, Keima570) and red light (maximum fluorescence 620 nm, Keima-Red), respectively. Both have large Stokes shift. They are suitable for multicolor fluorescence imaging, because they are hardly affected by other colors. It is also suitable for multi-wavelength measurement by single-wavelength excitation by a laser source.

Keima570 CoralHue™ Fluorescent Protein
Kogure T et al. A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nature Biotechnology. 24: 577-581 (2006) (PMID: 16648840)
Abbr. Form Excitation maxima (nm) Emission maxima (nm) S1 vector# MC1 vector$ MN1 vector$ MCLinker vector* MNLinker vector*
dKeima570 Dimer 440 570 RDB18294        
hdKeima570 Dimer 440 570 RDB18295     RDB18296 RDB18293

Keima-Red

Keima-Red CoralHue™ Fluorescent Protein
Kogure T et al. A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nature Biotechnology. 24: 577-581 (2006) (PMID: 16648840)
Abbr. Form Excitation maxima (nm) Emission maxima (nm) S1 vector# MC1 vector$ MN1 vector$ MCLinker vector* MNLinker vector*
dKeima-Red Dimer 440 616 RDB18285        
mKeima-Red Monomer 440 620 RDB18280   RDB18281    
hdKeima-Red Dimer 440 616 RDB18286     RDB18287 RDB18284
hmKeima-Red Monomer 440 620 RDB18282     RDB18283 RDB18279
Protein Resource (cat.num.) Reference
Keima-red tdKeima/pRSETB (RDB15232)
mKeima/pcDNA3 (RDB15233)
dKeima/pRSETB (RDB15234)
dKeima570/pRSETB (RDB15235) 		A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy.
Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581 (2006). PubMed PMID 16648840.
Keima-red mKeima (h)/pCS2 (RDB15236)
dKeima (h)/pCS2 (RDB15237) 		A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery.
Katayama, H. et al., Chem. Biol. 18 (8): 1042-1052 (2011). PubMed PMID 21867919.

NpF2164g5_BV4

Protein Resource (cat.num.) Reference
NpF2164g5_BV4 pCAG-EGFP-NpF2164g5_BV4 (RDB17331) Rational conversion of chromophore selectivity of cyanobacteriochromes to accept mammalian intrinsic biliverdin.
Fushimi, K., et al., Proc. Natl. Acad. Sci. U.S.A. 116 (17): 8301-8309 (2019). PubMed PMID 30948637.

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Photoconversion type

Kaede

Emiting green light and can be changed irreversibly its fluorescence to red by irradiation with UV or violet (360 nm-410 nm).

Kaede CoralHue™ Fluorescent Protein
Ando R et al. An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc Natl Acad Sci U S A. (2002) 99(20):12651-6. (PMID: 12271129)
Abbr. Form Excitation maxima (nm) Emission maxima (nm) S1 vector# MC1 vector$ MN1 vector$ MCLinker vector* MNLinker vector*
Kaede Tetramer 508/572 518/580 RDB18241 RDB18242 RDB18243    
Protein Resource (cat.num.) Reference
Kaede Kaede/pRSETB (RDB15231) An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein.
Ando, R. et al., Proc. Natl. Acad. Sci. U.S.A. 99 (20): 12651-12656 (2002). PubMed PMID 12271129.

Kikume Green-Red

Emiting green light and can be changed irreversibly its fluorescence to red by irradiation with UV or violet (360 nm-410 nm). Shows higher photoconversion efficiency than Kaede.

Kikume Green-Red CoralHue™ Fluorescent Protein
Habuchi S et al. mKikGR, A monomeric photoswitchable fluorescent protein. PLoS ONE 3, e3944 (2008) (PMID: 19079591)
Tsutsui H. et al. Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6, 233-238 (2005) (PMID: 15731765)
Abbr. Form Excitation maxima (nm) Emission maxima (nm) S1 vector# MC1 vector$ MN1 vector$ MCLinker vector* MNLinker vector*
KikGR1 Tetramer 507/583 517/593 RDB18272 RDB18273 RDB18274    
mKikGR1 Monomer 505/580 517/591 RDB18304        
hKikGR1 Tetramer 507/583 517/593 RDB18275 RDB18276 RDB18277 RDB18278 RDB18271
hmKikGR1 Monomer 505/580 517/591       RDB18305 RDB18303
Protein Resource (cat.num.) Reference
Kikume Green-Red KikGR/pRSETB (RDB15259) Semi-rational engineering of a coral fluorescent protein into an efficient highlighter.
Tsutsui, H. et al., EMBO Rep. 6 (3): 233-238 (2005). PubMed PMID 15731765.
Kikume Green-Red mKikGR/pRSETB (RDB15261) mKikGR, a monomeric photoswitchable fluorescent protein.
Habuchi, S. et al., PLoS One 3 (12): e3944 (2008). PubMed PMID 19079591.
h41pkve6 (derivative of Kikume Green-Red) Xpa (RDB15264)
Xpa H62Q (RDB15265) 		A diffraction-quality protein crystal processed as an autophagic cargo.
Tsutsui, H. et al., Mol. Cell 58 (1): 186-193 (2015). PubMed PMID 25773597.

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Photochromism type

Dronpa Green

Emiting green light and can be turned fluorescence on and off. It can be bleached by strong irradiation at about 500 nm. Bleached Dronpa Green can be restored by irradiation at about 400 nm. It is suitable for super-resolution microscope observation by Photoactivated localization microscopy (PALM) method.

Dronpa Green CoralHue™ Fluorescent Protein
Ando R et al. Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306, 1370-1373 (2004) (PMID: 15550670)
Abbr. Form Excitation maxima (nm) Emission maxima (nm) S1 vector# MC1 vector$ MN1 vector$ MCLinker vector* MNLinker vector*
DG1 Monomer 503 518 RDB18267 RDB18268 RDB18269    
hDG1 Monomer 503 518       RDB18270 RDB18266
DG3 Monomer 491 514 RDB18297        
Protein Resource (cat.num.) Reference
Dronpa-Green Dronpa/pRSETB (RDB15256)
22G/pRSETB (RDB15255) 		Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting.
Ando, R. et al., Science 306 (5700): 1370-1373 (2004). PubMed PMID 15550670.
Dronpa-Green Dronpa2/pRSETB (RDB15257)
Dronpa3/pRSETB (RDB15258) 		Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants.
Ando, R. et al., Biophys. J. 92 (12): L97-99 (2007). PubMed PMID 17384059.

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Timer type

Kusabira Green Orange

Protein Resource (cat.num.) Reference
Kusabira Green Orange mK-GO (RDB15244) Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein.
Tsuboi, T. et al., Mol. Biol. Cell. 21 (1): 87-94 (2010). PubMed PMID 19889833.

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Destabilized- and Secreted-FPs

Protein Resource (cat.num.) Reference
CFP pdsCFP (RDB15993) (destabilized FP) Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells.
Ando, H. et al., Sci. Rep. 7 (1): 12642 (2017). PubMed PMID 28974737.
GFP pdsGFP (RDB15992) (destabilized FP) Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells.
Ando, H. et al., Sci. Rep. 7 (1): 12642 (2017). PubMed PMID 28974737.
Venus pdsVenus (RDB15994) (destabilized FP) Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells.
Ando, H. et al., Sci. Rep. 7 (1): 12642 (2017). PubMed PMID 28974737.

 

Protein Resource (cat.num.) Reference
GFP pMRX-bsr-ss-sfGFP (RDB19002) (secreted FP) Knockout analysis of Rab6 effector proteins revealed the role of VPS52 in the secretory pathway.
Homma, Y., Fukuda, M., Biochem0 Biophys. Res. Commun. 561: 151-157 (2021). PubMed PMID 34023780.

FPs with signal sequence and tags

Protein Resource (cat.num.) Reference
GFP pcDNA3.1-myc-GFP (RDB14213) (myc-tagged FP) Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus.
Nishi, K. et al., RNA 19 (1): 17-35 (2013). PMID 23150874. (original).
GFP pCMFlag_EGFP (RDB06071) (FLAG-tagged FP) unpublished

 

Non-fluorescent chromoprotein

cjBlue

Protein Resource (cat.num.) Reference
cjBlue cjBlue/pRSETB (RDB15242)
cjBlue Y64L/pRSETB (RDB15243) 		Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus.
Chan, M.C. et al., J. Biol. Chem. 281 (49): 37813-37819 (2006). PubMed PMID 17028187.

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Thermostable fluorescent protein

  • Sea cactus thermostable green fluorescent protein, CoGFP
    Thermostable fluorescent protein and its utility.
    Murai M, Ogoh K, Kinebuchi T, Suzuki H.
    Japan patent unregistered 2014-60947.


    Upper, before heating. Lower, after heating at 100℃ for 10 minutes.
    Observed at room temperature.     		Relative fluorescent intencity.
      25℃ 70℃ 100℃
    GFP of A. victoria 1072 1087 629
    WT 4770 4229 703
    Th1wt 8067 7236 2162
    Th2wt 7668 7525 3711

     

    Catalog no. Name of clone Characteristic
    RDB14364 pCoGFP-Th1wt wild type
    RDB14366 pCoGFP-Th2wt wild type
    RDB14365 pCoGFP-Th1co Optimize codons for transfection into mammalian cells
    RDB14367 pCoGFP-Th2co Optimize codons for transfection into mammalian cells

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References

Sensor & Visualization

  • Sensor & Visualization
    • Bilirubin indicator UnaG, BReleaCa
    • cAMP indicator
    • Caspase activity indicator
    • pH sensor
    • Protein concentration in cells
    • RA indicator
    • Voltage indicator
  • Autophagy indicator
    • Chaperone mediated autophagy activity by the GAPDH-HT indicator by Dr. Takahiro Seki’s lab as well as fluorescent protein probes of LC3B accumulation developed by Dr. Noboru Mizushima’s lab, Dr. Itaru Hamachi’s lab and Dr. Keiji Kimura’s lab are available.
  • Calcium-ion sensor
    • Fluorescent protein-based Ca2+ sensors, G-CaMPs, Yellow Cameleons and Pericams developed by Dr. Jin-ichi Nakai’s lab and Dr. Atsushi Miyawaki’s lab, which are composed with calmodulin, fluorescent protein and M13 peptide (CaM binding domain of myosin light chain kinase), are designated to visualize intracellular [Ca2+] dynamics.
    • G-CaMP
    • Pericam
    • Yellow Cameleon
  • Cell cycle indicator Fucci
    • To monitor cell cycle progression in living cells, cell cycle indicator Fucci probes deveoped by Dr. Atsushi Miyawaki’s lab are available.
  • Epigenetics reporter
    • Visualization of histone acetylation: The Histac fluorescent probes deposited by Dr. Kazuki Sasaki allow you monitoring the state of activity of acetylation of histone H4 by fluorescence in living cells.
    • Visualization of methylated DNA: The EGFP-MBD-nls protein recognizes the methylated DNA and you can follow status of the DNA methylation in situ under physiological conditions using the pEGFP-MBD-nls expression clone.
  • Knock in markers with CRISPR/Cas9 genome editing
    • As a part of Auxin Inducible Degron (AID) System clones, Dr. Masato Kanemaki provides a series of knock-in fluorescent and selection markers with CRISPR/Cas9 genome editing.
  • Notch signaling reporter
    • The pRBS-EGFP and RBP-J-Venus expression clones deposited by Dr. Makoto Mark Taketo and Dr. Kenji Tanigaki, respectively, allow you monitoring the state of activation of the Notch signaling by fluorescence in living cells.
  • Organelle marker/subcellular localization
  • Sphingolipid marker
    • Lipid rafts are small lipid domains on the cell membrane and are thought to play an important role in signal transduction, endocytosis and more. We provide fluorescent probes for sphingomyelin and cholesterol lipid domains.
    • Nakanori: sphingomyelin and cholesterol lipid domain (lipid raft)
    • D4 toxin: cholesterol rich domain
    • lysenin: sphingomyelin

Luminescent protein

  • Nano-lantern luminescent and fluorescent protein
    • Nano lantern (NL) consists of Renilla luciferase and adjacent fluorescence protein. The chemiluminescence of the luciferase provides light source for excitation and enables the fluorescence protein to be observed. Three colors of NLs, yellow, cyan and orange, have been developed. NLs do not require external light source and overcome problems such as autofluorescence, phototoxicity, and photobleaching.
  • Higher intensity luciferases having Green-, Yellow- or Red-emission by using D-luciferin
    • These luciferases have at most four-time maximum luminous intensity than that of widely used luciferase of the Photinus pyralis, a common North American firefly. Further more, Green-, Yellow- or Red-emission can be obtained by using D-luciferin as a substrate of each luciferase.
  • AkaLuc luciferase providing brighter and red-shifted luminescence
    • The artificial bioluminescence system AkaBLI enables noninvasive signal observation in deep tissue of living animals. It was developed by Dr. Atsushi Miyawaki and Dr. Satoshi Iwano of the RIKEN Center for Brain Science, and Dr. Shojiro Maki of the University of Electro-Communications. The AkaBLI consists of an artificial substrate AkaLumine with improved tissue permeability and an artificial luciferase Akaluc optimized to AkaLumine. The intensity of the luminescence of AkaBLI system is 100 to 1000 folds brighter than the conventional systems.

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(GRP0050e 2013.06.23 T.M.)

2022.05.13



 

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