Fluorescent protein resource
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Cyan | Green | Yellow | Orange | Red
photoconversion type | photochromism type | Non-fluorescent chromoprotein
Tools | Thermostable FP | Sensor & Visualization | Luminescent protein
Cyan
Kusabira Cyan
Emitting bright cyan light. There is less overlap of fluorescence spectra on yellow than ECFP.
Kusabira-Cyan CoralHue™ Fluorescent Protein | ||||||||
Abbr. | Form | Excitation maxima (nm) | Emission maxima (nm) | S1 vector# | MC1 vector$ | MN1 vector$ | MCLinker vector* | MNLinker vector* |
---|---|---|---|---|---|---|---|---|
KCy1 | Dimer | 453 | 486 | RDB18310 |
Midoriishi Cyan
Emitting cyan light. There is less overlap of fluorescence spectra on yellow than ECFP
Midoriishi-Cyan CoralHue™ Fluorescent Protein | ||||||||
Abbr. | Form | Excitation maxima (nm) | Emission maxima (nm) | S1 vector# | MC1 vector$ | MN1 vector$ | MCLinker vector* | MNLinker vector* |
---|---|---|---|---|---|---|---|---|
MiCy1 | Dimer | 472 | 495 | RDB18265 | ||||
mMiCy1 | Monomer | 470 | 496 | RDB18289 | ||||
hmMiCy1 | Monomer | 470 | 496 | RDB18290 | RDB18291 | RDB18292 | RDB18288 |
Protein | Resource (cat.num.) | Reference |
---|---|---|
Midoriishi-Cyan | mMiCy1/pRSETB (RDB15241) | Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312 (2004). PubMed PMID 15065984. |
CFP
Protein | Resource (cat.num.) | Reference |
---|---|---|
CFP | pdsCFP (RDB15993) (destabilized FP) | Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Ando, H. et al., Sci. Rep. 7 (1): 12642 (2017). PubMed PMID 28974737. |
Green
Umikinoko Green
Emitting brilliant green light. It has high pH stability.
Umikinoko-Green CoralHue™ Fluorescent Protein | ||||||||
Abbr. | Form | Excitation maxima (nm) | Emission maxima (nm) | S1 vector# | MC1 vector$ | MN1 vector$ | MCLinker vector* | MNLinker vector* |
---|---|---|---|---|---|---|---|---|
mUKG1 | Monomer | 483 | 499 | RDB18306 | ||||
hmUKG1 | Monomer | 483 | 499 | RDB18307 | RDB18308 | RDB18309 |
Azami Green
Emitting green light.
Azami-Green CoralHue™ Fluorescent Protein | ||||||||
Abbr. | Form | Excitation maxima (nm) | Emission maxima (nm) | S1 vector# | MC1 vector$ | MN1 vector$ | MCLinker vector* | MNLinker vector* |
---|---|---|---|---|---|---|---|---|
AG | Tetramer | 492 | 505 | RDB18244 | ||||
mAG1 | Monomer | 492 | 505 | RDB18246 | RDB18247 | RDB18248 | ||
hmAG1 | Monomer | 492 | 505 | RDB18249 | RDB18250 | RDB18251 | RDB18252 | RDB18245 |
hmAG407 | Monomer | 407 | 498 | RDB18328 |
Protein | Resource (cat.num.) | Reference |
---|---|---|
Azami Green | hmAG407/pRSETB (RDB15229) hmAG1/pRSETB (RDB15230). |
A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. Karasawa, S. et al., J. Biol. Chem. 278 (36): 34167-34171 (2003). PubMed PMID 12819206. |
h2-3
Protein | Resource (cat.num.) | Reference |
---|---|---|
h2-3 | h2-3/pRSETb (RDB18219) | Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. Ando, R. et al., bioRxiv (2020) doi: https://doi.org/10.1101/2020.03.30.015156. |
GFP
Protein | Resource (cat.num.) | Reference |
---|---|---|
GFP | pdsGFP (RDB15992) (destabilized FP) | Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Ando, H. et al., Sci. Rep. 7 (1): 12642 (2017). PubMed PMID 28974737. |
Yellow
Achilles
Protein | Resource (cat.num.) | Reference |
---|---|---|
Achilles | Achilles/pRSETB (RDB15982) | Coupling delay controls synchronized oscillation in the segmentation clock. Yoshioka-Kobayashi, K. et al., Nature. 2020 Jan 8. doi: 10.1038/s41586-019-1882-z. [Epub ahead of print]. PMID 31915376. |
Venus
Protein | Resource (cat.num.) | Reference |
---|---|---|
Venus | Venus/pCS2 (RDB15116) mVenus/pRSETB (RDB15117) |
A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nagai, T. et al., Nat. Biotechnol. 20 (1): 87-90 (2002). PubMed PMID 11753368. |
Venus | cp49Venus (RDB15120) cp145Venus (RDB15121) cp157Venus (RDB15122) cp173Venus (RDB15123) cp195Venus (RDB15124) cp229Venus (RDB15125) |
Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Nagai, T. et al., Proc. Natl. Acad. Sci. U.S.A. 101 (29): 10554-10559 (2004). PubMed PMID 15247428. |
Venus | pdsVenus (RDB15994) (destabilized FP) | Time-lapse imaging of microRNA activity reveals the kinetics of microRNA activation in single living cells. Ando, H. et al., Sci. Rep. 7 (1): 12642 (2017). PubMed PMID 28974737. |
Cy11.5
Protein | Resource (cat.num.) | Reference |
---|---|---|
Cy11.5 | Cy11.5/pRSETB (RDB15706) | Concatenation of cyan and yellow fluorescent proteins for efficient resonance energy transfer. Shimozono, S. et al., Biochemistry 45 (20): 6267-6271 (2006). PubMed PMID 16700538. |
Orange
Kusabira Orange
Emitting orange light. It has pH stability. Its sharp fluorescence spectrum enables multicolor fluorescence imaging with red fluorescent proteins which are difficult to use other orange fluorescent protein.
Kusabira-Orange CoralHue™ Fluorescent Protein | ||||||||
Abbr. | Form | Excitation maxima (nm) | Emission maxima (nm) | S1 vector# | MC1 vector$ | MN1 vector$ | MCLinker vector* | MNLinker vector* |
---|---|---|---|---|---|---|---|---|
KO1 | Dimer | 548 | 561 | RDB18253 | ||||
mKO1 | Monomer | 548 | 559 | RDB18258 | RDB18259 | RDB18260 | ||
mKO2 | Monomer | 551 | 565 | RDB18299 | ||||
hKO1 | Dimer | 548 | 561 | RDB18254 | RDB18255 | RDB18256 | ||
hmKO1 | Monomer | 548 | 559 | RDB18261 | RDB18262 | RDB18263 | RDB18264 | RDB18257 |
hmKO2 | Monomer | 551 | 565 | RDB18300 | RDB18301 | RDB18302 | RDB18298 |
Protein | Resource (cat.num.) | Reference |
---|---|---|
Kusabira Orange | hmKO1/pRSETB (RDB15312) hmKO2/pRSETB (RDB15313) |
Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Karasawa, S. et al., Biochem. J. 381 (Pt 1): 307-312 (2004). PubMed PMID 15065984. |
Kusabira Orange | hmKO-K/pRSETB (RDB15314) | Improving membrane voltage measurements using FRET with new fluorescent proteins. Tsutsui, H. et al., Nat. Methods 5 (8): 683-685 (2008). PubMed PMID 18622396. |
Red
AzaleaB5
Protein | Resource (cat.num.) | Reference |
---|---|---|
AzaleaB5 | AzaleaB5/pRSETb (RDB18218) | Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. Ando, R. et al., bioRxiv (2020) doi: https://doi.org/10.1101/2020.03.30.015156. |
Keima570 and Keima-Red
Emitting orange light (maximum fluorescence 570 nm, Keima570) and red light (maximum fluorescence 620 nm, Keima-Red), respectively. Both have large Stokes shift. They are suitable for multicolor fluorescence imaging, because they are hardly affected by other colors. It is also suitable for multi-wavelength measurement by single-wavelength excitation by a laser source.
Keima570 CoralHue™ Fluorescent Protein | ||||||||
Abbr. | Form | Excitation maxima (nm) | Emission maxima (nm) | S1 vector# | MC1 vector$ | MN1 vector$ | MCLinker vector* | MNLinker vector* |
---|---|---|---|---|---|---|---|---|
dKeima570 | Dimer | 440 | 570 | RDB18294 | ||||
hdKeima570 | Dimer | 440 | 570 | RDB18295 | RDB18296 | RDB18293 |
Keima-Red
Keima-Red CoralHue™ Fluorescent Protein Kogure T et al. A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nature Biotechnology. 24: 577-581 (2006) (PMID: 16648840) |
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Abbr. | Form | Excitation maxima (nm) | Emission maxima (nm) | S1 vector# | MC1 vector$ | MN1 vector$ | MCLinker vector* | MNLinker vector* |
---|---|---|---|---|---|---|---|---|
dKeima-Red | Dimer | 440 | 616 | RDB18285 | ||||
mKeima-Red | Monomer | 440 | 620 | RDB18280 | RDB18281 | |||
hdKeima-Red | Dimer | 440 | 616 | RDB18286 | RDB18287 | RDB18284 | ||
hmKeima-Red | Monomer | 440 | 620 | RDB18282 | RDB18283 | RDB18279 |
Protein | Resource (cat.num.) | Reference |
---|---|---|
Keima-red | tdKeima/pRSETB (RDB15232) mKeima/pcDNA3 (RDB15233) dKeima/pRSETB (RDB15234) dKeima570/pRSETB (RDB15235) |
A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Kogure, T. et al., Nat. Biotechnol. 24 (5): 577-581 (2006). PubMed PMID 16648840. |
Keima-red | mKeima (h)/pCS2 (RDB15236) dKeima (h)/pCS2 (RDB15237) |
A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Katayama, H. et al., Chem. Biol. 18 (8): 1042-1052 (2011). PubMed PMID 21867919. |
NpF2164g5_BV4
Protein | Resource (cat.num.) | Reference |
---|---|---|
NpF2164g5_BV4 | pCAG-EGFP-NpF2164g5_BV4 (RDB17331) | Rational conversion of chromophore selectivity of cyanobacteriochromes to accept mammalian intrinsic biliverdin. Fushimi, K., et al., Proc. Natl. Acad. Sci. U.S.A. 116 (17): 8301-8309 (2019). PubMed PMID 30948637. |
Photoconversion type
Kaede
Emiting green light and can be changed irreversibly its fluorescence to red by irradiation with UV or violet (360 nm-410 nm).
Kaede CoralHue™ Fluorescent Protein Ando R et al. An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc Natl Acad Sci U S A. (2002) 99(20):12651-6. (PMID: 12271129) |
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Abbr. | Form | Excitation maxima (nm) | Emission maxima (nm) | S1 vector# | MC1 vector$ | MN1 vector$ | MCLinker vector* | MNLinker vector* |
---|---|---|---|---|---|---|---|---|
Kaede | Tetramer | 508/572 | 518/580 | RDB18241 | RDB18242 | RDB18243 |
Protein | Resource (cat.num.) | Reference |
---|---|---|
Kaede | Kaede/pRSETB (RDB15231) | An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Ando, R. et al., Proc. Natl. Acad. Sci. U.S.A. 99 (20): 12651-12656 (2002). PubMed PMID 12271129. |
Kikume Green-Red
Emiting green light and can be changed irreversibly its fluorescence to red by irradiation with UV or violet (360 nm-410 nm). Shows higher photoconversion efficiency than Kaede.
Kikume Green-Red CoralHue™ Fluorescent Protein Habuchi S et al. mKikGR, A monomeric photoswitchable fluorescent protein. PLoS ONE 3, e3944 (2008) (PMID: 19079591) Tsutsui H. et al. Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6, 233-238 (2005) (PMID: 15731765) |
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Abbr. | Form | Excitation maxima (nm) | Emission maxima (nm) | S1 vector# | MC1 vector$ | MN1 vector$ | MCLinker vector* | MNLinker vector* |
---|---|---|---|---|---|---|---|---|
KikGR1 | Tetramer | 507/583 | 517/593 | RDB18272 | RDB18273 | RDB18274 | ||
mKikGR1 | Monomer | 505/580 | 517/591 | RDB18304 | ||||
hKikGR1 | Tetramer | 507/583 | 517/593 | RDB18275 | RDB18276 | RDB18277 | RDB18278 | RDB18271 |
hmKikGR1 | Monomer | 505/580 | 517/591 | RDB18305 | RDB18303 |
Protein | Resource (cat.num.) | Reference |
---|---|---|
Kikume Green-Red | KikGR/pRSETB (RDB15259) | Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. Tsutsui, H. et al., EMBO Rep. 6 (3): 233-238 (2005). PubMed PMID 15731765. |
Kikume Green-Red | mKikGR/pRSETB (RDB15261) | mKikGR, a monomeric photoswitchable fluorescent protein. Habuchi, S. et al., PLoS One 3 (12): e3944 (2008). PubMed PMID 19079591. |
h41pkve6 (derivative of Kikume Green-Red) | Xpa (RDB15264) Xpa H62Q (RDB15265) |
A diffraction-quality protein crystal processed as an autophagic cargo. Tsutsui, H. et al., Mol. Cell 58 (1): 186-193 (2015). PubMed PMID 25773597. |
Photochromism type
Dronpa Green
Emiting green light and can be turned fluorescence on and off. It can be bleached by strong irradiation at about 500 nm. Bleached Dronpa Green can be restored by irradiation at about 400 nm. It is suitable for super-resolution microscope observation by Photoactivated localization microscopy (PALM) method.
Dronpa Green CoralHue™ Fluorescent Protein Ando R et al. Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science 306, 1370-1373 (2004) (PMID: 15550670) |
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Abbr. | Form | Excitation maxima (nm) | Emission maxima (nm) | S1 vector# | MC1 vector$ | MN1 vector$ | MCLinker vector* | MNLinker vector* |
---|---|---|---|---|---|---|---|---|
DG1 | Monomer | 503 | 518 | RDB18267 | RDB18268 | RDB18269 | ||
hDG1 | Monomer | 503 | 518 | RDB18270 | RDB18266 | |||
DG3 | Monomer | 491 | 514 | RDB18297 |
Protein | Resource (cat.num.) | Reference |
---|---|---|
Dronpa-Green | Dronpa/pRSETB (RDB15256) 22G/pRSETB (RDB15255) |
Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Ando, R. et al., Science 306 (5700): 1370-1373 (2004). PubMed PMID 15550670. |
Dronpa-Green | Dronpa2/pRSETB (RDB15257) Dronpa3/pRSETB (RDB15258) |
Highlighted generation of fluorescence signals using simultaneous two-color irradiation on Dronpa mutants. Ando, R. et al., Biophys. J. 92 (12): L97-99 (2007). PubMed PMID 17384059. |
Timer type
Kusabira Green Orange
Protein | Resource (cat.num.) | Reference |
---|---|---|
Kusabira Green Orange | mK-GO (RDB15244) | Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. Tsuboi, T. et al., Mol. Biol. Cell. 21 (1): 87-94 (2010). PubMed PMID 19889833. |
Non-fluorescent chromoprotein
cjBlue
Protein | Resource (cat.num.) | Reference |
---|---|---|
cjBlue | cjBlue/pRSETB (RDB15242) cjBlue Y64L/pRSETB (RDB15243) |
Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. Chan, M.C. et al., J. Biol. Chem. 281 (49): 37813-37819 (2006). PubMed PMID 17028187. |
Thermostable fluorescent protein
- Sea cactus thermostable green fluorescent protein, CoGFP
Thermostable fluorescent protein and its utility.
Murai M, Ogoh K, Kinebuchi T, Suzuki H.
Japan patent unregistered 2014-60947.
Upper, before heating. Lower, after heating at 100℃ for 10 minutes.
Observed at room temperature.Relative fluorescent intencity. 25℃ 70℃ 100℃ GFP of A. victoria 1072 1087 629 WT 4770 4229 703 Th1wt 8067 7236 2162 Th2wt 7668 7525 3711 Catalog no. Name of clone Characteristic RDB14364 pCoGFP-Th1wt wild type RDB14366 pCoGFP-Th2wt wild type RDB14365 pCoGFP-Th1co Optimize codons for transfection into mammalian cells RDB14367 pCoGFP-Th2co Optimize codons for transfection into mammalian cells
References
Sensor & Visualization
- Sensor & Visualization
- Bilirubin indicator UnaG, BReleaCa
- cAMP indicator
- Caspase activity indicator
- pH sensor
- Protein concentration in cells
- RA indicator
- Voltage indicator
- Autophagy indicator
- Chaperone mediated autophagy activity by the GAPDH-HT indicator by Dr. Takahiro Seki’s lab as well as fluorescent protein probes of LC3B accumulation developed by Dr. Noboru Mizushima’s lab, Dr. Itaru Hamachi’s lab and Dr. Keiji Kimura’s lab are available.
- Calcium-ion sensor
- Fluorescent protein-based Ca2+ sensors, G-CaMPs, Yellow Cameleons and Pericams developed by Dr. Jin-ichi Nakai’s lab and Dr. Atsushi Miyawaki’s lab, which are composed with calmodulin, fluorescent protein and M13 peptide (CaM binding domain of myosin light chain kinase), are designated to visualize intracellular [Ca2+] dynamics.
- G-CaMP
- Pericam
- Yellow Cameleon
- Cell cycle indicator Fucci
- To monitor cell cycle progression in living cells, cell cycle indicator Fucci probes deveoped by Dr. Atsushi Miyawaki’s lab are available.
- Epigenetics reporter
- Visualization of histone acetylation: The Histac fluorescent probes deposited by Dr. Kazuki Sasaki allow you monitoring the state of activity of acetylation of histone H4 by fluorescence in living cells.
- Visualization of methylated DNA: The EGFP-MBD-nls protein recognizes the methylated DNA and you can follow status of the DNA methylation in situ under physiological conditions using the pEGFP-MBD-nls expression clone.
- Knock in markers with CRISPR/Cas9 genome editing
- As a part of Auxin Inducible Degron (AID) System clones, Dr. Masato Kanemaki provides a series of knock-in fluorescent and selection markers with CRISPR/Cas9 genome editing.
- Notch signaling reporter
- The pRBS-EGFP and RBP-J-Venus expression clones deposited by Dr. Makoto Mark Taketo and Dr. Kenji Tanigaki, respectively, allow you monitoring the state of activation of the Notch signaling by fluorescence in living cells.
- Organelle marker/subcellular localization
- We are providing genetic resources for visualization of organelles such as mitochondria and nucleus. Each clone contains an organelle localization signal sequence fused with fluorescent proteins or epitope tags. Organelles can be detected by fluorescence or by detecting epitope tags with antibodies.
- Visualization of organelle contact sites using the organelle-targeted split-GFP system developed by Dr. Yasushi Tamura
- Sphingolipid marker
- Lipid rafts are small lipid domains on the cell membrane and are thought to play an important role in signal transduction, endocytosis and more. We provide fluorescent probes for sphingomyelin and cholesterol lipid domains.
- Nakanori: sphingomyelin and cholesterol lipid domain (lipid raft)
- D4 toxin: cholesterol rich domain
- lysenin: sphingomyelin
Luminescent protein
- Nano-lantern luminescent and fluorescent protein
- Nano lantern (NL) consists of Renilla luciferase and adjacent fluorescence protein. The chemiluminescence of the luciferase provides light source for excitation and enables the fluorescence protein to be observed. Three colors of NLs, yellow, cyan and orange, have been developed. NLs do not require external light source and overcome problems such as autofluorescence, phototoxicity, and photobleaching.
- Higher intensity luciferases having Green-, Yellow- or Red-emission by using D-luciferin
- These luciferases have at most four-time maximum luminous intensity than that of widely used luciferase of the Photinus pyralis, a common North American firefly. Further more, Green-, Yellow- or Red-emission can be obtained by using D-luciferin as a substrate of each luciferase.
- AkaLuc luciferase providing brighter and red-shifted luminescence
- The artificial bioluminescence system AkaBLI enables noninvasive signal observation in deep tissue of living animals. It was developed by Dr. Atsushi Miyawaki and Dr. Satoshi Iwano of the RIKEN Center for Brain Science, and Dr. Shojiro Maki of the University of Electro-Communications. The AkaBLI consists of an artificial substrate AkaLumine with improved tissue permeability and an artificial luciferase Akaluc optimized to AkaLumine. The intensity of the luminescence of AkaBLI system is 100 to 1000 folds brighter than the conventional systems.
(GRP0050e 2013.06.23 T.M.)
2021.03.26