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Bioengineering misc

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Bioengineering misc

Blasticidin S deaminase

  • Cloning of the blasticidin S deaminase gene (BSD) from Aspergillus terreus and its use as a selectable marker for Schizosaccharomyces pombe and Pyricularia oryzae.
    Kimura M, Kamakura T, Tao QZ, Kaneko I, Yamaguchi I.
    Mol. Gen. Genet. 242 (2): 121-129 (1994). PMID 8159161.

    Catalog no. Name of resource Descriptiion
    RDB19904 pBSA712 Expression vector of lacZ:BSD (blasticidin-S deaminase) fusion in E. coli.
    RDB19905 pBF101 Expression vector of BSD (blasticidin-S deaminase) in a filamentous fungus, such as Pyricularia oryzae.

Nanoparticle

  • Multiplexed 129Xe HyperCEST MRI Detection of Genetically Reconstituted Bacterial Protein Nanoparticles in Human Cancer Cells.
    Mizushima R, Inoue K, Fujiwara H, Iwane AH, Watanabe TM, Kimura A.
    Contrast Media Mol. Imaging 2020: 5425934 (2020). PMID 32256252.

    Catalog no. Name of resource Descriptiion
    RDB16904 pT2b_mT_gvpC16-T2A-EGFP_pP_rm Tol2 transposon expression vector of Planktothrix rubescens gvpC16 and EGFP marker (C).
    RDB16905 pT2b_mT_gvpC20-T2A-mKO2_pP_rm Tol2 transposon expression vector of Planktothrix rubescens gvpC20 and mKO2 marker (C).
    RDB16906 pT2b_mT_gvpC28-T2A-EGFP_pP_rm Tol2 transposon expression vector of Planktothrix agardhii gvpC28 and EGFP marker (C).

Streptavidin

  • Streptavidin-aequorin fusion protein for bioluminescent immunoassay.
    Inouye S, Sato J, Sasaki S, Sahara Y.
    Biosci. Biotechnol. Biochem. 75 (3): 568-571 (2011). PMID 21389603.

    Catalog no. Name of resource Descriptiion
    RDB19843 pSTA51 Expression vector of S. avidinii Streptavidin.

ZZ domain of Staphylococcal Protein A

  • Soluble protein expression in E. coli cells using IgG-binding domain of protein A as a solubilizing partner in the cold induced system.
    Inouye S, Sahara Y.
    Biochem. Biophys. Res. Commun. 376 (3): 448-453 (2008). PMID 18789309.

    Catalog no. Name of resource Descriptiion
    RDB19844 pJN-ZZ Expression vector of ZZ domain (Synthetic IgG-binding domain from Staphylococcal Protein A).

Artificial enzymatic RNA editing system

  • RNA editing of BFP, a point mutant of GFP, using artificial APOBEC1 deaminase to restore the genetic code.
    Bhakta S, Sakari M, Tsukahara T.
    Sci. Rep. 10 (1): 17304 (2020). PMID 33057101.

    Catalog no. Name of resource Descriptiion
    RDB19359 Target BFP gene expression plasmid Expression vector of target BFP gene, a point mutant (T199C) of GFP for artificial enzymatic RNA editing system.
    RDB19360 pCS2+MT-MS2HB-APOBEC1 Expression vector of the catalytic domain of human APOBEC1 tagged with MS2HB at N-terminus for artificial enzymatic RNA editing system.
    RDB19361 MS2-guideRNA21bp Expression vector of guideRNA, a 21 ntd sequence complementary to the target BFP mRNA tagged with MS2 stem loop at C-terminus for artificial enzymatic RNA editing system.

ChIP-seq analysis

  • ChIP-seq analysis of genomic binding regions of five major transcription factors highlights a central role for ZIC2 in the mouse epiblast stem cell gene regulatory network.
    Matsuda, K., Mikami, T., Oki, S., Iida, H., Andrabi, M., Boss, J.M., Yamaguchi, K., Shigenobu, S., Kondoh, H.
    Development 144 (11): 1948-1958 (2017). PubMed PMID 28455373.

    Catalog no. Name of resource Descriptiion
    RDB15774 pCAGGS-BLRP-BirA Expression vector to perform ChIP-seq analysis using biotinylated proteins.

    ChIP-seq is a powerful method of comprehensive analysis of transcription factor (TF) binding sites. However, the bottle neck of ChIP-seq analysis is the quality of antibody, and thus many researchers have been given up. To overcome, authors constructed the vector expressing “biotinylated-tag” transcription factors. With this vector, you can get acceptable level of TF-DNA complex for ChIP-seq analysis by streptavidin-beads for any kinds of TF.

Open-Sandwich Immunoassay (OS-IA)

  • Homogeneous Noncompetitive Luminescent Immunodetection of Small Molecules by Ternary Protein Fragment Complementation.
    Ohmuro-Matsuyama, Y., Ueda, H.
    Anal. Chem. 90 (5): 3001-3004 (2018). PubMed PMID 29446920.

    Catalog no. Name of resource Descriptiion
    RDB16003 pET-LnBiT Bacterial expression vector of LnBiT, a comporntnt of the Open Sandwich Bioluminescent Immunoassay (OS-BLIA).

Thermostable kanamycin for microbial gene disruption

  • Directed evolution of thermostable kanamycin-resistance gene: a convenient selection marker for Thermus thermophilus.
    Hoseki, J., Yano, T., Koyama, Y., Kuramitsu, S., Kagamiyama, H.
    J. Biochem. 126 (5): 951-956 (1999). PubMed PMID 10544290.

    Catalog no. Name of resource Descriptiion
    RDB03436 pUC18-HTK (with promoter) Expression vector of Thermus highly thermostable kanamycin nucleotidyltransferase (HTK) gene

    Depositor: Dr. Jun Hoseki

2025.05.04 (T.M.)



 

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