Import Quarantine Material

Current information relevant to adenovirus

Type of adenovirus Human adenovirus type 5
Treatment of material PRIOR to importation into your country (processing/purification methods, including time at specific temperatures, pH, other treatment, disease safeguard, etc.) The cells infected with recombinant adenovirus were sonicated and frozen at -80oC.
Cell line or reference number (for the cells used to produce the adenovirus) HEK293 obtained from ATCC.
Cell line passage history Within 20 passages after obtaining from ATCC. The cells were cultured in Japan and dispatched from Japan.
Type of culture medium (basic or special) DMEM
Identify nutritive factors in medium (protein/nitrogen sources used and/or added: specify country of origin, in particular in the case of supplements or nitrogen sources of animal origin) Supplemented with 5% fetal bovine serum (FBS) that was inactivated by incubation at 56oC for 45 min. The FBS (Invitrogen/Life Technologies) was imported from Australia to Japan. This FBS is derived from animals that received ante and post mortem inspection at the time of slaughter and were found free of infectious diseases.
Country of origin and sources of enzymes used (e.g. trypsin) Trypsin, Canada (Invitrogen)
Names of any animal viruses studied in the lab in which the cell line originated The cells were not exposed to infectious agents of agricultural importance.
If recombinant, specify genetic insert Refer to the web catalog for specific information about each recombinant virus.



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