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RIKEN BioResource Research Center DNA Bank Mail News No. 129 (2023.06.05)
[hp] https://dna.brc.riken.jp/en/mailen/mailnewsen
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This E-mail news is sent to those who use the RIKEN BRC Gene Engineering Division (DNA Bank) and those who have subscribed to the E-mail news.
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1. Focused Resource
2. Conference exhibition
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1. Focused Resource
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High intensity forms of luciferase and luminescent proteins from various
organisms
– In nature, there are many bioluminescent organisms such as fireflies and a part of marine life. The bioluminescence is a result of oxidation of substrates. Wide variety of research methods applying bioluminescence, such as reporter assays, have been developed and used widely.
– Of reporter proteins, luciferase from the Photinus pyralis is a well-known luminescent enzyme, but in recent years, new luminescent enzymes have been derived from various marine organisms such as copepods and shrimp and artificially designed luminescent enzymes also have been developed. They have smaller molecular weight and higher intensity than that from fireflies. These characteristics less affect the function of proteins of interest fused to them, and the luminescent proteins are applied to imaging research observing the real-time dynamics of the target proteins in living cells.
– JNC CORPORATION (JNC), which sold bioluminescent plasmids developed in the course of bioluminescence research, deposited 27 plasmid DNAs carrying luminescent protein genes such as from Gaussia princeps, Oplophorus gracilirostris and Aquorin from Aequorea victoria. We will take over them and start to provide.
– Dr. KIM, Sung Bae and his colleagues at the National Institute of Advanced Industrial Science and Technology (AIST, Japan) have developed Artificial luciferase (ALuc), which is based on a luminescent enzymes derived from copepods and artificially reconstituted with optimal amino acid sequences. ALuc shows 100 times higher intensity and prolonged luminescence than original enzymes (Kim, S.B. et al. Bioconjug. Chem., 2013). They have deposited two types of ALuc plasmids to us this time.
– Other available products include red-shifted luciferase (Akaluc) and luciferase that is less susceptible to environmental factors (PmatLuc1, PsagRE1Luc, etc.). Choose according to your research application. We look forward to receiving your orders!
– Reference
Inouye, S. et al. Proc. Natl. Acad. Sci. U S A. 82 (10): 3154-3158, 1985. PMID 3858813
Inouye, S. et al. Protein Expr. Purif. 109: 47-54, 2015. PMID 25665506
Inouye, S. et al. Protein Expr. Purif. 52: 66-73, 2007. PMID 16997571
Inouye, S. FEBS Lett. 347 (2-3): 163-168, 1994. PMID 8033996
Inouye, S. et al. FEBS Lett. 315 (3): 343-346, 1993. PMID 8422928
Inouye, S. J. Biochem. 143 (5):711-717, 2008. PMID 18296715
Inouye, S. et al. FEBS Lett. 341 (2-3): 277-280, 1994. PMID 8137953
Inouye, S. et al. FEBS Lett. 481 (1): 19-25, 2000. PMID 10984608
Inouye, S. et al. Biochem. Biophys. Res. Commun. 445 (1): 157-162, 2014. PMID 24491536
Inouye, S. et al. Biochem. Biosci. Biotechnol. 75 (3): 568-571, 2011. PMID 21389603
Inouye, S. et al. Biochem. Biophys. Res. Commun. 376 (3): 448-453, 2008. PMID 18789309
Suzuki, T. et al. Anal. Bichem. 415 (2): 182-189, 2011. PMID 21477579
Suzuki, T. et al. Plos One. 6 (9): e25243, 2011. PMID 21969874
Inouye, S. et al. Protein Expr. Purif. 128: 93-100, 2016. PMID 27506135
Inouye, S. & Sahara, Y. Protein Expr. Purif. 53 (2): 384-389, 2007. PMID 17275329
Kim, S.B. et al. Bioconjug. Chem. 24 (12): 2067-2075, 2013. PMID 24237362
Kim, S.B. et al. ACS Comb. Sci. 19 (9): 594-599, 2017. PMID 28742969
– Clone information
JNC CORPORATION’s Resource
URL: https://dnaconda.riken.jp/search/depositor/dep104979.html
Sung-Bae Kim’s Resource
URL: https://dnaconda.riken.jp/search/depositor/dep100041.html
Higher intensity luciferase
URL: https://dna.brc.riken.jp/en/resource200en/rdb14359en
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2. Conference exhibition
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We will have a booth on the following schedule. Please visit our booth when you attend the conferences. If you are searching for appropriate genetic materials for your researcher, please feel free to ask us. May be able to help you.
– The 8th Annual Meeting of the Japanese Society for Genome Editing
Date: Jun. 6 (Tue) – Jun. 8 (Thu), 2023.
Venue: Tower Hall Funabori 1F, Exhibition Hall
Learn more: https://www2.aeplan.co.jp/ge2023/en_home/
– The 50th Annual Meeting of the Japanese Society of Toxicology
Date: Jun. 19 (Mon) – Jun. 21 (Wed), 2023.
Venue: Pacifico Yokohama JAPAN Conference Center 3F, NBRP booth, No.86
Learn more: https://jsot2023.jp/en/index.html
– The 75th Annual Meeting of the Japanese Society for Cell Biology
Date: Jun. 28 (Wed) – Jun. 30 (Fri), 2023.
Venue: Nara Prefectural Convention Center 1F, Convention Hall
Learn more: https://synonis.com/jscb2023
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RIKEN BioResource Research Center DNA Bank
3-1-1 Koyadai, Tsukuba Ibaraki, 305-0074 Japan
[e-mail] dna_sec.brc@riken.jp
[hp] https://dna.brc.riken.jp/en/
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2023.06.05.
