DNA Bank Mail News 2022 Sep 22

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RIKEN BioResource Research Center DNA Bank Mail News No. 126 (2022.09.22)
[hp] https://dna.brc.riken.jp/en/mailen/mailnewsen
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This E-mail news is sent to those who use the RIKEN BRC Gene Engineering Division (DNA Bank) and those who have subscribed to the E-mail news.

— CONTENTS —
1. Focused Resource
2. Conference exhibition
3. ANRRC 2022 ONLINE

1. Focused Resource

– StayGold, highly photostable GFP

– Since the discovery of green fluorescent protein (GFP) from Aequorea victoria, many fluorescent proteins with various wavelength ranges have been developed and used as visualization tools for analyzing the localization and dynamics of target genes in cells. However, fluorescent proteins have the weakness that they fade under strong or repeated excitation light exposure.

– A collaborative research group led by Dr. Atsushi Miyawaki of RIKEN Neuroscience Research Center and Tohoku University, Kitasato University, and Kao Corporation has isolated a novel GFP, CU17S gene derived from the jellyfish Cytaeis uchidae. They introduced the CU17S gene into cultured cells and E. coli and found that it was highly photostable. Furthermore, they succeeded in substantially increasing the brightness of the fluorescent protein without compromising its photostability by introducing a specific mutation in the CU17S gene, and this fluorescent protein was called “StayGold” (Hirano, M. et al., Nat. Biotechnol. 2022. PMID: 35468954).

– The photostability of StayGold is up to hundredfold better than existing fluorescent proteins. Taking advantage of the photostability of StayGold, Dr. Miyawaki and his colleagues conducted time-dependent observations to reveal structural changes in intracellular organelles. In addition, by combining StayGold with VHH antibody to spike protein of SARS-CoV-2, they succeeded in observing the maturation process of virus particles in infected cells.

– Dr. Miyawaki has deposited a total of 10 plasmids, including StayGold (cat# RDB19607: (n1)oxStayGold/pRSET, cat# RDB19608: (n1)oxStayGold(c4)/pRSET), which can fuse target genes to the N-terminus or C-terminus and tandem dimer StayGold (cat# RDB19611: tdoxStayGold/pcDNA3). We look forward to receiving your orders!

– Article
Hirano, M. et al. A highly photostable and bright green fluorescent protein. Nat. Biotechnol. 40 (7): 1132-1142 (2022).
doi: 10.1038/s41587-022-01278-2. PMID 35468954.

– Clone information
https://brc.riken.jp/dna/GRP0071e#EXR0118e
(n1)oxStayGold/pRSET (cat# RDB19607)
(n1)oxStayGold(c4)/pRSET (cat# RDB19608)
tdoxStayGold/pcDNA3 (cat# RDB19611)
tdoxStayGold(c4)/pBS Coupler (cat# RDB19612)

2. Conference exhibition

We will have a booth on the following schedule. If you are searching for appropriate genetic materials for your researcher, please feel free to ask us. May be able to help you. Please visit our booth when you attend the conferences.

– The 81st Annual Meeting of the Japanese Cancer Association Date: Sep. 29 (Thu) – Oct. 1 (Sat), 2022.
Venue: Pacific Convention Plaza Yokohama (PACIFICO Yokohama), Exhibition Hall (hall B and C, No.84)
Learn more: https://site.convention.co.jp/jca2022/en/

3. ANRRC 2022 ONLINE

The 13th International Meeting of the Asian Network of Research Resource Centers (ANRRC 2022), hosted by RIKEN BioResource Research Center, will be held online.

Date: Nov. 8 (Tue) – Oct. 9 (Wed), 2022.
Learn more: https://anrrc.info/anrrc2022/

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RIKEN BioResource Research Center DNA Bank
3-1-1 Koyadai, Tsukuba Ibaraki, 305-0074 Japan
[e-mail] dna_sec.brc@riken.jp
[hp] https://dna.brc.riken.jp/en/
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2022.09.22.



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