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RIKEN BioResource Research Center DNA Bank Mail News No. 125 (2022.05.17)
[hp] https://dna.brc.riken.jp/en/mailen/mailnewsen
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This E-mail news is sent to those who use the RIKEN BRC Gene Engineering Division (DNA Bank) and those who have subscribed to the E-mail news.
— CONTENTS —
1. Focused Resource
2. SARS-CoV-2 genes
1. Focused Resource
– Mammalian Expression Vector, pEF-BOS series
pEF-BOS and pEF-BOS-EX are mammalian expression vectors developed by Dr. Nagata Shigekazu of Immunology Frontier Research Center, Osaka University. These vectors carry the human elongation factor one alpha promoter (EF-1 alpha) with high promoter activity independent of cell type and are used in gene transfer experiments into various cell types.
We will now provide the new modified pEF-BOS-EX vectors deposited by Dr. Nagata. pEF-Flag-EX is utilized to express gene products with a FLAG-tag. pNEF-BOS-EX and pPEF-BOS-EX with neomycin and puromycin resistance markers, respectively, are utilized to establish stable expression cell lines. We are looking forward to receiving your request for pEF-BOS vector series.
– Article
Mizushima, S and Nagata, S. Nucleic Acids Res. 18 (17): 5322, 1990.
PMID: 1698283.
Murai, K. et al. Proc. Natl. Acad. Sci. USA. 95 (7): 3461-3466, 1998.
PMID: 9520388.
– clone information
https://brc.riken.jp/dna/GRP0071e#EXR0117e
pEF-BOS (cat# RDB07939)
pEF-BOS-EX (cat# RDB07940)
pEF-Flag-EX (cat# RDB18972)
pNEF-BOS-EX (cat# RDB18970)
pPEF-BOS-EX (cat# RDB18971)
2. SARS-CoV-2 genes
RIKEN BioResource Research Center (BRC) is a unique repository in the world, as it collects and distributes bioresources of three different levels, i.e., genetic materials (genomic and cDNA clones) and cell lines of human and animal origins as well mouse strains. With this unique feature, RIKEN BRC hopes to contribute to the comprehensive research and development on COVID-19.
Gene Engineering Division plays a role as a core facility of genetic material such as genomic and cDNA clones of human, animal and microbe origin, and genetic research tools such as genome editing vectors, fluorescent/ luminescent protein genes and viral vectors.
We have now started to provide two SARS-CoV-2 clones: cDNA clone encoding the ORF2 (spike protein) and genomic clone cording ORF4 to 10.
We are looking forward to receiving your request.
Bioresources for COVID-19 research in RIKEN BRC
[hp] https://web.brc.riken.jp/en/archives/news/20201029_02
SARS-CoV-2 genes and COVID-19 relevant human genes
[hp] https://brc.riken.jp/dna/GSB0049e
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RIKEN BioResource Research Center DNA Bank
3-1-1 Koyadai, Tsukuba Ibaraki, 305-0074 Japan
[e-mail] dna_sec.brc@riken.jp
[hp] https://dna.brc.riken.jp/en/
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2022.05.17.
