DNA Bank Mail News 2020 Jun 15

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RIKEN BioResource Research Center DNA Bank Mail News No. 120 (2021.06.15)
[hp] https://dna.brc.riken.jp/en/mailen/mailnewsen
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This E-mail news is sent to those who use the RIKEN BRC Gene Engineering Division (DNA Bank) and those who have subscribed to the E-mail news.

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1. Focused Resource
2. New available resources
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1. Focused Resource
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– Mitophagy visualization fluorescent sensor, mito-SRAI

Mitophagy is a selective autophagy degrading stress-damaged mitochondria.

Dr. Miyawaki Atsushi and Dr. Katayama Hiroyuki of RIKEN Center for Brain Science (CBS) and Dr. Hioki Hiroyuki of Juntendo University and their colleagues have developed mito-SRAI, a fluorescent sensor that can quantitatively visualize mitophagy both in live and fixed conditions.
The mito-SRAI is composed of TOLLES (cyan) fluorescent protein that is resistant to acidic condition and protein degradation in lysosomes, and YPet (yellow) fluorescent protein whose fluorescence intensity changes according to pH. The mito-SRAI is specifically localized in mitochondria and the mitophagy can be observed by representative ratio (TOLLES/YPet) images by expressing in cultured cells. The mito-SRAI has been genetically engineered to confer tolerance of fluorescence in fixed conditions.

The mito-SRAI might support drug discovery assays testing a huge amount of fixed biological samples and experimental animal studies (Katayama, H. et al., Cell, 181 (5): 1176-1187, 2020. PMID: 32437660).

– clone information
mito-SRAI_pcDNA3 (cat# RDB18223)
https://brc.riken.jp/dna/GSB0021e

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2. New available resources
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The following DNA materials have become newly available from us. We are looking forward to hearing your order!

– Dr. Takayuki Iwaki, Department of Pharmacology, Hamamatsu University School of Medicine.
A vector for expressing Nanobody (single chain antibody) in E. coli.
Iwaki, T. et al., Protein Expr. Purif., 170: 105607, 2020. PMID 32062022
– Resource and article information
https://rrc.nbrp.jp/references/61150

– Dr. Hiroaki Yuasa, Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Nagoya City University.
Transporter PCFT expression clone.
Nakai, Y. et al., J. Pharmacol. Exp. Ther., 322 (2): 469-476, 2007.
PMID 17475902
Inoue, K. et al., Am. J. Physiol. Gastrointest. Liver Physiol., 294 (3): G660-668, 2008. PMID 18174275
Yamashiro, T. et al., Sci. Rep., 9 (1): 18105, 2019. PMID 31792273
– Resource and article information
https://rrc.nbrp.jp/references/61151
https://rrc.nbrp.jp/references/61152
https://rrc.nbrp.jp/references/61153

– Dr. Nozomu Nish, Life Science Research Center Kagawa University.
Galectin expression vectors for E. coli.
Nishi, N. Glycoforum, 23 (5), A15, 2020.
– Resource and article information
https://rrc.nbrp.jp/references/61020
https://brc.riken.jp/dna/GSB0048e

– Dr. Toshiaki Watanabe, Central Institute For Experimental Animals.
P53 dominant negative clones for in vitro transcription.
Watanabe, T. et al., Genes Cells, 24 (7): 473-484, 2019. PMID 31099158
– Resource and article information
https://rrc.nbrp.jp/references/62517

– Dr. Masato Kanemaki, Molecular Cell Engineering Laboratory, National Institute of Genetics.
Auxin-inducible degron (AID) system clone
Yesbolatova, A. et al., Nat. Commun., 11 (1): 5701, 2020. PMID 33177522
– Resource and article information
https://rrc.nbrp.jp/references/63909
https://brc.riken.jp/dna/GRP0016e

– Dr. Shigeaki Aoki, Laboratory of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Chiba University.
Expression vector encoding Keima-Red that targets mitochondria.
Shiratori, R. et al., Sci. Rep., 9 (1): 18699, 2019. PMID 31822748
– Resource and article information
https://rrc.nbrp.jp/references/62699

– Dr. Kentaro Miyazaki, Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST).
Cloning vectors (counter selection).
Miyazaki, K. et al., Biotechniques 58 (2): 86-88 (2015). PMID 25652032
– Resource and article information
https://rrc.nbrp.jp/references/58474
https://brc.riken.jp/dna/GRP0048e

– Dr. Hidekazu Iioka, Niigata University Graduate School of Medical and Dental Science.
Expression vector of Crb3 and FGFR.
Iioka, H. et al., Int. J. Cancer, 145 (10): 2740-2753, 2019.
PMID 30980524
– Resource and article information
https://rrc.nbrp.jp/references/62682

– Dr. Mitsuhiro Iwaki, RIKEN Center for Biosystems Dynamics Research.
Expression vector of ACTN1 and subfragment 1 (S1) of MYH2.
Fujita, K. et al., Commun. Biol., 2: 437, 2019. PMID 31799438
– Resource and article information
https://rrc.nbrp.jp/references/62697

– Dr. Yasuhiro Yamada, Institute of Medical Science, University of Tokyo.
Expression vector of Cas9 with puromycin resistance gene.
Sakakura, M. et al., Biochem. Biophys. Res. Commun., 519 (4):
705-713, 2019. PMID 31543342
– Resource and article information
https://rrc.nbrp.jp/references/63313

– Medical & Biological Laboratories (MBL) Co., Ltd.
Coral Hue (TM), fluorescent protein clones.
– Resource and article information
[hp] https://dna.brc.riken.jp/en/mailen/tec20200928_01en

– Dr. Fukuda Mitsunori, Department of Integrative Life Sciences, Graduate School of Life Sciences, Tohoku University.
Expression vector of Rab family protein.
Oguchi, M. E. et al., J. Biol. Chem., 295 (36): 12674-12685, 2020.
PMID 32669361
– Resource and article information
https://rrc.nbrp.jp/references/63318

– Dr. Yasushi Tamura, Faculty of Science, Yamagata University.
A split-GFP clone that visualize the organelles interaction site in living cells.
Kakimoto Y. et al., Sci. Rep., 8 (1): 6175, 2018. PMID 29670150
– Resource and article information
https://rrc.nbrp.jp/references/53900

– Dr. Susumu Imaoka, Department of Biomedical Chemistry, School of Science and Technology, Kwansei Gakuin University.
Expression vector of genes involved in Nuclear-cytoplasmic transport.
Oguro, A. et al., Sci. Rep., 9 (1): 15296, 2019. PMID 31653923
– Resource and article information
https://rrc.nbrp.jp/references/63319

– Dr. Eisuke Itakura, Department of Biology, Graduate school of Science, Chiba University.
Fluorescent protein that monitors Lysosome activity.
Ishii, S. et al., Sci. Rep., 9 (1): 11635, 2019. PMID 31406169
– Resource and article information
https://rrc.nbrp.jp/references/63488

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RIKEN BioResource Research Center DNA Bank
3-1-1 Koyadai, Tsukuba Ibaraki, 305-0074 Japan
[e-mail] dna_sec.brc@riken.jp
[hp] https://dna.brc.riken.jp/en/
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2021.06.15



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