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RIKEN BioResource Research Center DNA Bank Mail News No. 116
[HP] https://dna.brc.riken.jp/en/mailen/mailnewsen
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This E-mail news is sent to those who use the RIKEN BRC Gene Engineering Division (DNA Bank) and those who have subscribed to the E-mail news.
:: CONTENTS ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
1. Focused Resource
2. New available resources
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1. Focused Resource
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– Achilles, a novel rapid-maturating fluorescent protein
For live imaging using fluorescent proteins, it is important to use a fluorescent protein with rapid maturation. Dr. Yusuke Niino and Dr. Atsushi Miyawaki of RIKEN Center for Brain Science (CBS) have developed Achilles which has shorter maturation time than Venus.
Dr. Ryoichiro Kageyama of Kyoto University and his colleagues have succeeded in analyzing the dynamics of the segmentation clock Hes7 protein, which has a short half-life, by using Achilles-Hes7 fusion protein (Yoshioka-Kobayashi, K. et al. Nature, 2020). Dr. Olivier Pourquie of Harvard Medical School and his colleagues established a cell line in which destabilized Achilles with a proteolytic signal was inserted into the 3 ‘end of the Hes7 gene. They were able to detect oscillatory expression of the Hes7-Achilles gene at the single-cell level. (Diaz-Cuadros, M. et al. Nature, 2020). These experiments became possible by utilizing the taking advantage of the rapid-maturation characteristics of Achilles. Achilles is available now from DNA Bank.
We are looking forward to hearing your order!
– clone information
Achilles_pRSETB (cat# RDB15982)
URL: https://brc.riken.jp/dna/GRP0055e#EXR0091e
– Reference
Yoshioka-Kobayashi, K. et al. Coupling delay controls synchronized oscillation in the segmentation clock. Nature, 2020. PMID: 31915376
Diaz-Cuadros, M. et al. In vitro characterization of the human segmentation clock. Nature, 2020. PMID: 31915384
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2. New available resources
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The following DNA materials have become newly available from us. We are looking forward to hearing your order!
– Depositor: Dr. Shiro Minami and Dr. Tomoko Nakata, Institute for Advanced Medical Sciences, Nippon Medical School.
Expression vector of rat liver activating protein (Lap), etc.
Nakata, T. et al., Endocr. J., 67 (2):185-200, 2020. PMID: 31748431
– clone information
https://rrc.nbrp.jp/references/58453
– Depositor: Dr. Takanori Amano, RIKEN BioResource Research Center Next Generation Human Disease Model Team.
Luciferase reporter for the mouse, rat and rabbit Sonic hedgehog (Shh) transcriptional regulatory regions, etc.
Tsukiji, N. et al., Mech. Dev., 131: 127-136, 2014. PMID: 24157522
– clone information
https://rrc.nbrp.jp/references/58303
– Depositor: Dr. Kentaro Miyazaki, Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST).
Disruption vector of 16s rRNA gene in Thermus thermophilus HB27
Miyazaki, K., Tomariguchi N., Sci. Rep., 9 (1): 11233, 2019.
PMID: 31375780
– clone information
https://rrc.nbrp.jp/references/58471
– Depositor: Dr. Atsushi Miyawaki and Dr. Hiroyuki Katayama, RIKEN Center for Brain Science
Fluorescent protein vectors for visualizing mitophagy
Katayama, H. et al., Cell, 181(5): 1176-1187.e16, 2020. PMID: 32437660
– clone information
https://rrc.nbrp.jp/references/60760
– Depositor: Dr. Atsushi Miyawaki, Dr. Asako Sakaue-Sawano and Dr. Ryoko Ando, RIKEN Center for Brain Science
New fluorescent proteins, AzaleaB5 and h2-3, and Fucci cell cycle indicator (tFucci(SA)5, tFucci(CA)5)
Ando, R. et al., bioRxiv, 2020.
– clone information
https://rrc.nbrp.jp/references/60761
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We hope you enjoyed receiving this message. However, if you would prefer to unsubscribe from receiving future email news from us, please send an email to dna_sec.brc@riken.jp.
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RIKEN BioResource Research Center DNA Bank
3-1-1 Koyadai, Tsukuba Ibaraki, 305-0074 Japan
[e-mail] dna_sec.brc@riken.jp
[HP] https://dna.brc.riken.jp/en/
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2020.08.28.
