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Takara Bio USA, Inc. and RIKEN BioResource Research Center concluded license agreement on preservation and distribution of Fluorescent Proteins, such as DsRed2 and mCherry for academic use

BRC News

DNA Bank Mail News No. 111


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RIKEN BioResource Research Center DNA Bank Mail News No. 111
[HP] https://dna.brc.riken.jp/en/mailen/mailnewsen
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== CONTENT ==
1. Focused Resource
2. New available resources
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This E-mail news is sent to those who use the RIKEN BRC Gene Engineering Division (DNA Bank) and those who have subscribed to the E-mail news.
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1. Focused Resource
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– Distribution of BAC clones
https://dna.brc.riken.jp/DataSheet/GRP0055e#EXR0107e

RIKEN BRC DNA Bank distributes the following Bacterial Artificial Chromosomes (BAC) clones; Mouse (C57BL/6N and MSM/Ms strains), Rat (F344/Stm and LE/Stm strains), Japanese macaque (M. fuscacta), and Drosophila flies (5 species, including D. melanogaster). These BAC clones can be searched by a gene symbols as a key word, and the position of the clone on the genome region can be visually confirmed.
We look forward to receiving your order.

BAC clones are used to construct gene knock out (KO) vectors and reporter genes having fluorescent proteins and LacZ to develop KO and transgenic animals. The BAC clones are also used for cloning the transcriptional regulatory region of genes. One of successful use of BAC clone is described in the following section.

– Glucocorticoid up-regulates PD-1 on T cells
T cell activation is known to be suppressed by an immunosuppressant Glucocorticoid (GC) and by inductively expressed PD-1.
Maeda, N. et al. found the enhancement of PD-1 expression by GC during their study of the effect of immunosuppressants on PD-1 expression. Furthermore, they found that the up-regulation of PD-1 was caused by the transcriptional activation of PD-1 via the GC response element on the transcription regulatory region located upstream of the PD-1 gene.
In their study, the genomic fragment of the transcriptional regulatory region of PD-1 used for this analysis was amplified by PCR from the B6N BAC clone B6Ng01-240G08 provided by us.

– Reference
Maeda, N. et al. Glucocorticoids potentiate the inhibitory capacity of programmed cell death 1 by up-regulating its expression on T cells. J. Biol. Chem., 294 (52): 19896-19906, 2019. PMID: 31723031

– clone information
[HP] https://dna.brc.riken.jp/en/cloneseten

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2. New available resources
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The following DNA materials have become newly available from us. We are looking forward to hearing your order!

– Depositor: Dr. Ian Jackson and Dr. Richard Mort, The University of Edinburgh.
An improved probe of the cell cycle indicator, Fucci.
Ford, MJ. et al., Dev. Cell, 47(4):509-523, 2018. PMID: 30458140
– clone information
https://rrc.nbrp.jp/references/54489

– Depositor: Dr. Takuhiro Ito, RIKEN Center for Biosystems Dynamics Research.
Expression vector of human translation initiation factor eIF2B.
Kashiwagi, K. et al., Science, 364 (6439):495-499, 2019.
PMID: 31048492
– clone information
https://rrc.nbrp.jp/references/58308

– Depositor: Dr. Mitsuo Tagaya, School of Life Sciences, Tokyo University of Pharmacy and Life Sciences.
Expression vector of rat MAP1B-light chain 1
K Arasaki, K. et al., EMBO Rep., 19 (8). pii: e45584, 2018.
PMID: 29925525
– clone information
https://rrc.nbrp.jp/references/58322

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RIKEN BioResource Research Center DNA Bank
3-1-1 Koyadai, Tsukuba Ibaraki, 305-0074 Japan
[HP] https://dna.brc.riken.jp/en/
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2020.03.05.