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Clontech Laboratories, Inc. and RIKEN BioResource Research Center concluded license agreement on preservation and distribution of Fluorescent Proteins, DsRed2 and mCherry for academic use

BRC News

DNA Bank Mail News No. 110


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RIKEN BioResource Research Center DNA Bank Mail News No. 110
[HP] https://dna.brc.riken.jp/en/mailen/mailnewsen
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CONTENT
1.Conference exhibition
2.Focused Resource
3.New available resources
4.Request for deposition of your genetic materials
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This E-mail news is sent to those who use the RIKEN BRC Gene Engineering Division (DNA Bank) and those who have subscribed to the E-mail news.

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1. Conference exhibition
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We will have a booth on the following schedule. If you are searching for appropriate genetic materials for your researcher, please feel free to ask us. May be able to help you. Please visit our booth when you attend the conferences.

– The 42nd Annual Meeting of the Molecular Biology Society of Japan
Date: Dec. 3 (Tue) ? Dec. 6 (Fri), 2019.
Place: Marine Messe Fukuoka arena 1F, NBRP corner in poster/exhibition hall
Learn more, URL: https://www2.aeplan.co.jp/mbsj2019/english/general/index.html

– The 48th Annual Meeting of the Japanese Society for Immunology
Date: Dec. 11 (Wed) ? Dec. 13 (Fri), 2019.
Place: ACT CITY Hamamatsu, In poster/exhibition event hall
Learn more, URL: http://icongroup.co.jp/48immunology/english/

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2. Focused Resource
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– Novel vectors for efficient PCR cloning
https://dna.brc.riken.jp/DataSheet/GRP0055e#EXR0101e

Novel three vectors for efficient cloning of PCR products by TA-cloning, blunt-end cloning and either cloning methods were recently developed and published in April of this year as described below, by Dr. Ken Motohashi of the Kyoto Sangyo University. These vectors are now available from us. We are looking forward to receiving your order!

Deposited resources :

– TA cloning vector (cat # RDB17479 pCRT)
By digestion with Xcm I restriction enzyme, it can be used for TA cloning.
clone information : http://dna.brc.riken.jp/DataSheet/RDB17479

– Blunt-end cloning vector (cat # RDB17481 pCRZero)
By digestion with EcoR V restriction enzyme, it can be used for blunt-end cloning.
clone information : http://dna.brc.riken.jp/DataSheet/RDB17481

– TA- and Blunt-end cloning vector (cat # RDB17480 pCRZeroT)
This vector can be used for the TA cloning or blunt-end cloning by digestion with either Xcm I or Sma I, respectively.
clone information : http://dna.brc.riken.jp/DataSheet/RDB17480

Reference :
Motohashi, K. A novel series of high-efficiency vectors for TA cloning and blunt-end cloning of PCR products. Sci. Rep. 9 (1): 6417, 2019. PMID: 31015513.

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– Fluorescent protein probe that can visualize the multiple inter-organelle contact sites in cells.
https://dna.brc.riken.jp/DataSheet/GRP0055e#EXR0090e

Split GFP-fragments have a feature that they can emit fluorescence again by reconstitution when both fragments are in close proximity.
When labelled organelles such as mitochondria and ERs by the divided GFP fragments exist together, the contact sites in cells can be visualized by the reconstituted fluorescent protein. The vectors of fluorescent protein probes developed by Dr. Yasushi Tamura of the Yamagata University, Dr. Toshiya Endo of the Kyoto Sangyo University and their colleague are available from the DNA Bank.

Reference :
Kakimoto, Y et al. Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep., 8 (1): 6175, 2018. PMID: 29670150.

DNA resource :
clone information : https://dna.brc.riken.jp/DataSheet/GRP0058e#rdb16037

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3. New available resources
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The following DNA materials have become newly available from the DNA Bank. We are looking forward to receiving your order!

– Depositor : Dr. Takata Minoru, Kyoto University.
Expression vectors of RFWD3, responsible gene of Fanconi anemia.
Inano, S. et al., Mol. Cell, 66 (5): 622-634, 2017. PMID: 28575658
clone information : https://rrc.nbrp.jp/references/54484

– Depositor : Dr. Nitta Ryo, Kobe University Graduate School of Medicine.
Constructs for biochemical analysis of proteins involved in axonal microtubule formation.
Sumi, T. et al. Cell Struct. Funct. 43 (1): 15-23, 2018. PMID: 29479005
clone information : https://rrc.nbrp.jp/references/57702

– Depositor : Dr. Narikawa Rei, Shizuoka University.
Biliverdin acceptable cyanobacteriochrome photoreceptors, NpF2164g5_BV4, emitting near-infrared fluorescence in mammalian cells and organs.
Fushimi, K. et al., Proc. Natl. Acad. Sci. U S A, 116 (17): 8301-8309, 2019. PMID: 30948637
clone information : https://rrc.nbrp.jp/references/57683

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4. Request for deposition of your genetic materials
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– Please deposit new genetic materials that you have developed to us. We also ask you to deposit modified genetic materials that were derived from our materials.

– Depositors of valuable genetic materials are appreciated and admired by the global research community. Deposition of your genetic materials leads to increasing citation of your original papers. Furthermore, you will contribute the progress of life science enormously.

– How to make a deposit
[HP] https://dna.brc.riken.jp/en/deposition

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We hope you enjoyed receiving this message. However, if you would rather not receive future e-mails from us, please send a blank email with ‘[RIKEN BRC] unsubscribe DNA Bank Mail News’ in the subject to dna_sec.brc@riken.jp from the email address you are subscribed under.
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RIKEN BioResource Research Center DNA Bank
3-1-1 Koyadai, Tsukuba Ibaraki, 305-0074 Japan
[e-mail] dna_sec.brc@riken.jp
[HP] https://dna.brc.riken.jp/en/
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2019.11.27.