Fluorescent probes for sphingomyelin and cholesterol lipid domains.(2018/02/06 N.N.)
- Lipid rafts are small lipid domains on the cell membrane and are thought to play an important role in signal transduction, endocytosis and more. Because of the lack of appropriate probes to label sphingomyelin and cholesterol individually, detailed analysis of lipid rafts was difficult.
- Dr. Toshihide Kobayashi and his colleagues of the former Lipid Biology Laboratory at RIKEN have developed methods to visualize the lipid rafts using probes of lipid binding proteins fused with fluorescent proteins.
- DNA Bank provides plasmids of the mCherry and GFP fluorescent probes that can label lipid rafts, sphingomyelin and cholesterol. We are looking forward to receiving your request soon.
- Reference
Abe, M. et al. Cell Rep. 37 (6): 109935, 2021.
Makino, A. et al. FASEB J., 31 (4): 1301-1322, 2017. [PMID: 27492925]
Makino, A. et al. FASEB J., 29 (2): 477-493, 2015. [PMID: 25389132]
Mizuno, H. et al. Chem. Sci. 2: 1548-1553, 2011.
Shimada, Y. et al. Eur. J. Biochem., 269 (24): 6195-6203, 2002. [PMID: 12473115] - Protocol
We hope the literature by Drs. Abe and Kobayashi will help you.
Abe, M., Kobayashi, T. Methods Cell Biol. 137: 15-24 (2017). [PMID: 28065303]
Markers
| Catalog no. | Name of clone | Running title |
|---|---|---|
| RDB13496 | pET28/EqtII-EGFP-His6 | Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of Eqinatoxin II. |
| RDB13497 | pET28/EqtII(8-69)-EGFP-His6 | Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Eqinatoxin II. |
| RDB13498 | pET28/His6-EGFP-NT-Lys | Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin. |
| RDB13499 | pET28/His6-mCherry-NT-Lys | Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin. |
| RDB13501 | pCAG/tdiRFP-CAAX | Marker of plasma membrane. Mammalian expression plasmid of CAAX box protein. |
| RDB13958 | pMAL/MBP-lysenin | Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of MBP-tagged lysenin. |
| RDB13959 | pQE30/His6-Venus-NT-Lys | Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of His6-Venus-tagged non toxic lysenin. |
| RDB13960 | pQE30/His6-mRFP-NT-Lys | Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of His6-mRFP-tagged non toxic lysenin. |
| RDB13961 | pET28/His6-EGFP-D4 | Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of His6-EGFP-tagged perfringolysin theta toxin D4 of C. perfringens. |
| RDB13962 | pET28/PlyA2-EGFP-His6 | Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged PlyA2. |
| RDB13963 | pET28/Oly-EGFP-His6 | Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged Oly. |
| RDB13964 | pET28/EryA-EGFP-His6 | Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged EryA. |
| RDB14300 | pET28/His6-mCherry-D4 | Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of His6-mCherry-tagged perfringolysin theta toxin D4 of C. perfringens. |
| RDB14301 | pET28/His6-EGFP-Nakanori | Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of His6-EGFP-tagged G. frondosa Nakaniori. |
| RDB15469 | pET28/Dronpa-theta-D4 | Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of perfringolysin theta toxin D4 of C. perfringens. |
| RDB15470 | pET28/Dronpa-NT-Lys | Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Lysenin. |
| RDB19208 | pHalo-NT-Lys | Mammalian expression vector of HaloTag(R) 7-tagged nontoxic Lysenin. |
| RDB19215 | pET28/His6-NT-Lys | Expression vector of His-tagged nontoxic Lysenin for E. coli. |
Sphingomyelinase C
| Catalog no. | Name of clone | Running title |
|---|---|---|
| RDB19219 | pTRE-SaSMaseC | Mammalian expression vector of Staphylococcus aureus sphingomyelinase C (SMase C), Tet-On/Off Advanced IRES mCherry system. |
Link to KEGG Pathway – Sphingolipid signaling pathway
List of gene resources
- Human gene resources [open/close]
- Mouse gene resources [open/close]
(GSB0027e, T.M. 2016.10.28)
2023.04.18
