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Clontech Laboratories, Inc. and RIKEN BioResource Research Center concluded license agreement on preservation and distribution of Fluorescent Proteins, DsRed2 and mCherry for academic use

BRC News

HLA Clones


HLA Clones

Outline

Alleles of human HLA class I, which are frequently observed in Japanese people, cloned into an expression vector were deposited by Dr. Yoshiki Akatsuka, Aichi Cancer Ctr. Res. Inst. The other classes of human HLA cDNA clones are also available.
Click for the list of available clones.

Related Information

EBV transformed B cell lines derived from Japanese (HEV) with information of HLA haplotypes

Japanese Society for Histocomaptibility and Immunogenetics

International Histocompatibility Workshop Group

HLA Infomatics Group – The Anthony Nolan Trust

Order Forms

Please specify catalog number (ex. RDB02867) of individual clone(s).
Please write Terms and Conditions for Distribution in MTA as indicated in a data sheet of each clone.
Please complete forms:

    Order Form, FormA (MS-Word)
    Material Transfer Agreement, FormC (pdf)
    Approval Form (Form D) (If indicated in a data sheet of each clone.)

 

Order Form (FormA)
Please complete the form with your shipping information including your account number of an international courier
(FedEx. World Courier, TNT Express, DHL Global Forwarding and others).
NOTE: User registration on our system is required before download the Order Form for credit card (Visa / Master).

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Material Transfer Agreement (Category I MTA)
For the use of our bioresource in research for not-for-profit academic purpose by a non-profit organization.

We ask a signature of the Authorized Representative of a recipient institution.
In the Section 2(a), please write your purpose of use of clone in 10 to 20 words.

  • Please ask us if applicable for the following use:
     1. For research to be conducted by for-profit organizations.
     2. For collaborative research between for-profit organization and not-for-profit organization.
     3, For research by not-for- organization outsourced and sponsored by for-profit organization.
     4. For for-profit research by not-for-profit organization including R&D with the aim of patent acquisition.
Word
Approval Form (Form D)
Please complete this form in the case Depositor and/or Developer require a written consent for distribution of material.
Please send this form to the depositor or developer for permission. Then, please send a photocopy of the completed
orm together with other forms to us.
MS-Word

Distribution fee per clone:
8,640 YEN (For use in research for not-for-profit academic purpose).
(Shipping cost is not included.)

For details for fee and payment, please visit Information of Request for Distribution.
Revision of distribution fees for the bioresources (Sep 18, 2019)

Please visit this page for further information of distribution.

Send forms:

  • Gene Engineering Division, RIKEN BioResource Research Center,
    3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan
    E-mail: dnabank.brc@riken.jp


 

HLA Class I

Antigen Allele Cat. No. Name of Clone Frequency in Japanese population
freq.1 (%) freq.2 (%) freq.3 (%) freq.4 (%)
HLA-A1 A*01:01 RDB03368 Human HLA-A*01:01 cDNA 1.8 0.2 0.57 0.25
HLA-A2 A*02:06 RDB02867 Human HLA-A*02:06 cDNA 8.4 10.4 8.99 8.74
HLA-A2 A*02:07 RDB02868 Human HLA-A*02:07 cDNA 4.0 3.4 2.87 3.39
HLA-A2 A*02:01:01 RDB02866 Human HLA-A*02:01:01 cDNA 10.6 10.9 10.71 11.64
HLA-A3 A*03:01 RDB03367 Human HLA-A*03:01 cDNA r 0.8 0.57 0.25
HLA-A11 A*11:01:01 RDB02869 Human HLA-A*11:01:01 cDNA 11.1 8.1 10.71 10.22
HLA-A24 RDB01176 pA01
HLA-A24 A*24:02:01 RDB02871 Human HLA-A*24:02:01 cDNA 32.7 35.6 36.52 36.20
HLA-A24 A*24:20 RDB03365 Human HLA-A*24:20 cDNA vr vr 0.83
HLA-A26 A*26:01 RDB02870 Human HLA-A*26:01 cDNA 6.2 9.8 11.28 7.66
HLA-A26 A*26:03 RDB03366 Human HLA-A*26:03 cDNA 1.3 2.1 1.91 1.47
HLA-A31 A*31:01:02 RDB02872 Human HLA-A*31:01:02 cDNA 9.3 7.7 6.88 9.14
HLA-A33 A*33:03 RDB02873 Human HLA-A*33:03 cDNA 12.8 7.9 7.84 6.68
98.2 96.9 98.85 96.47
Antigen Allele Cat. No. Name of Clone Frequency in Japanese population
freq.1 (%) freq.2 (%) freq.3 (%) freq.4 (%)
HLA-B7 B*07:02:01 RDB02874 Human HLA-B*07:02:01 cDNA 5.2 5.2 3.63 5.70
HLA-B8 B*08:01 RDB03369 Human HLA-B*08:01 cDNA
HLA-B15 B*15:01:01 RDB02875 Human HLA-B*15:01:01 cDNA 6.5 7.2 9.37 7.51
HLA-B35 B*35:01:01 RDB02876 Human HLA-B*35:01:01 cDNA 7.8 8.6 8.80 7.76
HLA-B38 B*38:01 RDB03370 Human HLA-B*38:01 cDNA
HLA-B39 B*39:01:01 RDB03371 Human HLA-B*39:01:01 cDNA 3.5 r 4.02 3.44
HLA-B40 B*40:01:02 RDB02877 Human HLA-B*40:01:02 cDNA 6.1 5.1 5.35 5.40
HLA-B40 B*40:02 RDB02878 Human HLA-B*40:02 cDNA 6.1 8.2 8.41 8.25
HLA-B44 B*44:01 RDB01178 AM2.60
HLA-B44 B*44:03:01 RDB02879 Human HLA-B*44:03:01 cDNA 12.2 6.9 6.69 6.24
HLA-B46 B*46:01 RDB02880 Human HLA-B*46:01 cDNA 6.1 3.8 3.44 4.96
HLA-B48 B*48:01 RDB03372 Human HLA-B*48:01 cDNA 4.3 2.7 5.54 2.65
HLA-B51 B*51:01:01 RDB02881 Human HLA-B*51:01:01 cDNA 7.0 7.9 6.88 8.35
HLA-B52 B*52:01:01 RDB02882 Human HLA-B*52:01:01 cDNA 10.4 13.7 0.19 10.90
HLA-B54 B*54:01 RDB03373 Human HLA-B*54:01 cDNA 7.0 6.5 7.07 8.79
HLA-B55 B*55:02 RDB03374 Human HLA-B*55:02 cDNA 2.2 3.2 2.87 1.92
HLA-B55 RDB01180 pB55
HLA-B59 B*59:01 RDB03375 Human HLA-B*59:01 cDNA 0.9 1.7 1.72 1.96
85.3 80.7 73.98 83.83
Antigen Allele Cat. No. Name of Clone Frequency in Japanese population
freq.1 (%) freq.2 (%) freq.3 (%) freq.4 (%)
HLA-Cw1 C*01:02 RDB02883 Human HLA-C*01:02 cDNA 17.0 16.63
HLA-Cw1 C*01:03 RDB03376 Human HLA-C*01:03 cDNA r
HLA-Cw3 C*03:03:01 RDB02884 Human HLA-C*03:03:01 cDNA 7.8 13.19
HLA-Cw3 C*03:04:01 RDB02885 Human HLA-C*03:04:01 cDNA 11.3 13.77
HLA-Cw4 C*04:01:01 RDB02886 Human HLA-C*04:01:01 cDNA 6.5 4.40
HLA-Cw6 C*06:02 RDB03377 Human HLA-C*06:02 cDNA 1.7 1.34
HLA-Cw7 C*07:02 RDB02887 Human HLA-C*07:02 cDNA 11.3 10.33
HLA-Cw8 C*08:01 RDB02888 Human HLA-C*08:01 cDNA 10.9 9.75
HLA-Cw12 C*12:02:02 RDB02889 Human HLA-C*12:02:02 cDNA 10.4 11.09
HLA-Cw14 C*14:02:01 RDB03378 Human HLA-C*14:02:01 cDNA 5.7 4.78
HLA-Cw14 C*14:03 RDB02890 Human HLA-C*14:03 cDNA 12.2 7.07
HLA-Cw15 C*15:02:01 RDB03379 Human HLA-C*15:02:01 cDNA 1.7 4.40
HLA-Cx 52 (Cw 12) C*12:01 RDB01177 pAC201
HLA-Cx52 (Cw12) C*12:01 RDB01179 pC68
96.5 96.75
HLA-G RDB01246 HLA-G.7.0 E

Freq.1: frequency in Japanese population (N=117), Tokunaga K. et al. Immunogenetics 1997; 46(3)199-205.
Freq.2: frequency in Japanese population (N=493), Tanaka H. et al. Clinical Transplants 1996; 139-144.
Freq.3: frequency in Japanese population (N=523), Nakajima, et al. MHC Vol.8 No.1 p1-32.
Freq.4: frequency in Japanese population (N=1018), Azuma F., et al., MHC, p166, 2004.
r: rare; vr: very rare.


Gene Symbol Product Name Synonyms
HLA-A major histocompatibility complex, class I, A HLAA
HLA-B major histocompatibility complex, class I, B AS|B-4901|HLAB
HLA-C major histocompatibility complex, class I, C D6S204|HLA-JY3|HLAC|HLC-C|MHC|PSORS1
HLA-E major histocompatibility complex, class I, E HLA-6.2|QA1
HLA-F major histocompatibility complex, class I, F CDA12|HLA-5.4|HLA-CDA12|HLAF
HLA-G major histocompatibility complex, class I, G MHC-G

HLA Class II

Antigen Allele Cat. No. Name of Clone
HLA DR beta 5 RDB01222 TOK H5 DR beta
HLA-DR beta 4 RDB01223 YT158
HLA-DQA1 RDB01227 pgDQ4A
HLA-DQB1 RDB01228 pg DQ1B
HLA-DQB1 RDB01230 pg DQ1BS
HLA-DRA RDB01232 DRA2EH
HLA-DPA1 RDB01233 DPA 02022
HLA-DPB1 RDB01234 DPB0202
HLA-DRB1 RDB01235 K^b DRB1 0803
HLA-DRB1 RDB01236 K^b DRB1 1201
HLA-DRB1 RDB01237 K^b DRB1 1302
HLA-DRB3 RDB01238 DRB30301 EMJ-4
HLA-DQA1 RDB01239 DQA10501 AMALA-4
HLA-DQB1 RDB01240 DQB10301 AMALA-4
HLA-DQA1 RDB01241 DQA10101 KAS116 3-6
HLA-DQB1 RDB01242 DQB10503 EK2-4

 


Gene Symbol Product Name Synonyms
HLA-DMA major histocompatibility complex, class II, DM alpha D6S222E|DMA|HLADM|RING6
HLA-DMB major histocompatibility complex, class II, DM beta D6S221E|RING7
HLA-DOA major histocompatibility complex, class II, DO alpha HLA-DNA|HLA-DZA|HLADZ
HLA-DOB major histocompatibility complex, class II, DO beta DOB|HLA_DOB
HLA-DPA1 major histocompatibility complex, class II, DP alpha 1 DP(W3)|DP(W4)|HLA-DP1A|HLADP|HLASB|PLT1
HLA-DPB1 major histocompatibility complex, class II, DP beta 1 DPB1|HLA-DP|HLA-DP1B|HLA-DPB
HLA-DPB2 major histocompatibility complex, class II, DP beta 2 (pseudogene) DP2B|DPB2|DPbeta2|HLA-DP2B
HLA-DQA1 major histocompatibility complex, class II, DQ alpha 1 CELIAC1|DQ-A1|HLA-DQA
HLA-DQB1 major histocompatibility complex, class II, DQ beta 1 CELIAC1|HLA-DQB|IDDM1
HLA-DQB2 major histocompatibility complex, class II, DQ beta 2 HLA-DQB1|HLA-DXB
HLA-DRA major histocompatibility complex, class II, DR alpha HLA-DRA1
HLA-DRB1 major histocompatibility complex, class II, DR beta 1 DRB1|HLA-DR1B|HLA-DRB|SS1
HLA-DRB3 major histocompatibility complex, class II, DR beta 3 HLA-DR1B|HLA-DR3B
HLA-DRB4 major histocompatibility complex, class II, DR beta 4 DR4|DRB4|HLA-DR4B
HLA-DRB5 major histocompatibility complex, class II, DR beta 5

REFERENCES FOR HLA

Akatsuka Y., Morishima Y., Kuzushima K., Kodera Y., Takahashi T. (2007) Clinical relevance of a newly identified HLA-A24-restricted minor histocompatibility antigen epitope derived from BCL2A1, ACC-1, in patients receiving HLA genotypically matched unrelated bone marrow transplant.
Cancer Science 98 (8): 1139-1146.

Nishida T, Akatsuka Y, Morishima Y, Hamajima N, Tsujimura K, Kuzushima K, Kodera Y, Takahashi T. (2004) Clinical relevance of a newly identified HLA-A24-restricted minor histocompatibility antigen epitope derived from BCL2A1, ACC-1, in patients receiving HLA genotypically matched unrelated bone marrow transplant.
Br. J. Haematol. 124 (5): 629-635.

Akatsuka Y, Nishida T, Kondo E, Miyazaki M, Taji H, Iida H, Tsujimura K, Yazaki M, Naoe T, Morishima Y, Kodera Y, Kuzushima K, Takahashi T. (2003) Identification of a Polymorphic Gene, BCL2A1, Encoding Two Novel Hematopoietic Lineage-specific Minor Histocompatibility Antigens.
J. Exp. Med. 197 (11): 1489-1500.

Akatsuka Y, Goldberg TA, Kondo E, Martin EG, Obata Y, Morishima Y, Takahashi T, Hansen JA (2002) Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines.
Tissue Antigens, 59 (6): 502-511.

Akatsuka Y, Kondo E, Taji H, Morishima Y, Yazaki M, Obata Y, Kodera Y, Riddell SR, Takahashi T. (2002) Targeted cloning of cytotoxic T cells specific for minor histocompatibility antigens restricted by HLA class I molecules of interest.
Transplantation, 74: 1773-1780.

Ando H, Miyata Y, Takata H, Inoko H. (1993) Identification of alloantisera reacting with HLA-C blank (Cx52) using a mouse L-cell transfected with the HLA-Cw*1201 allele.
Hum Immunol. 36 (2): 76-80

Senju S, Kimura A, Yasunami M, Kamikawaji N, Yoshizumi H, Nishimura Y, Sasazuki T. (1992) Allele-specific expression of the cytoplasmic exon of HLA-DQB1 gene.
Immunogenetics 36 (5): 319-325.

Zemmour J, Parham P. (1991) HLA class I nucleotide sequences, 1991.
Tissue Antigens 37 (4): 174-180. Erratum in: Tissue Antigens. 1992 Nov;40(5):221-228.

Fujisawa K, Kamikawaji N, Yasunami M, Kimura A, Nishimura Y, Sasazuki T. (1991) High precursor frequency of human T cells reactive to HLA-DQ molecules expressed on mouse L cell transfectants.
Eu.r J Immunol. 21 (10): 2341-2347.

Kamikawaji N, Fujisawa K, Yoshizumi H, Fukunaga M, Yasunami M, Kimura A, Nishimura Y, Sasazuki T. (1991) HLA-DQ-restricted CD4+ T cells specific to streptococcal antigen present in low but not in high responders.
J Immunol. 146 (8): 2560-2567.

Tamaki J. (1992)[Analysis of polymorphism at the human class I MHC locus, HLA-G]
Hokkaido Igaku Zasshi (Hokkaido J. Med. Sci.) 67 (5): 583-594. Japanese.

Nishimura Y, Iwanaga T, Inamitsu T, Yanagawa Y, Yasunami M, Kimura A, Hirokawa K, Sasazuki T. (1990) Expression of the human MHC, HLA-DQW6 genes alters the immune response in C57BL/6 mice.
J Immunol. 1990 Jul 1;145(1):353-60.

Takata H, Inoko H, Ando A, Haranaka M, Watanabe B, Tsuji K, Iri H. (1988) Cloning and analysis of HLA class I cDNA encoding a new HLA-C specificity Cx52.
Immunogenetics 28 (4): 265-270.

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2018.05.31