p53 dependent and independent G1 arrest
DNA damaging agent activates the kinases named Atm and Atr that phosphorylate chk1 and chk2. The phosphorylation of p53 on ser20 by chks induces p53 stabilization by inhibiting MDM2 binding. p21induced by p53 binds to Cyclin E/cdk2 complex to make it inactive form.Chks also help ubiquitination and degradation of CDC25A that is required for activation of Cyclin E/cdk2 complex. (Contributed by Dr. Koji Nakade)
Reporters
List of gene resources
| Locus | RDB no. | Type | Name of clone | Running title |
|---|---|---|---|---|
| Trp53 | 1285 | DNA clone | p53-LTR pUC18 (Anti Sense#9) | Expression vector, Antisense p53, cDNA |
| Trp53 | 1284 | DNA clone | p53-LTR pUC18 (Sense#3) | Expression vector, Sense p53, cDNA |
| TP53 | 5507 | DNA clone | pKM2L-phP53 | Promoter Bank clone, Human p53 promoter |
| TP53 | 3187 | Recombinant adenovirus | AxCAhp53KP1065 | Recombinant adenovirus harboring human p53 cDNA (Ser96Pro) |
| TP53 | 3186 | Recombinant adenovirus | AxCAhp53KP1010 | Recombinant adenovirus harboring human p53 cDNA (Gly105Asp) |
| TP53 | 3185 | Recombinant adenovirus | AxCAhp53KP1007 | Recombinant adenovirus harboring human p53 cDNA (Gly374Cys) |
| TP53 | 3184 | Recombinant adenovirus | AxCAhp53KP1094 | Recombinant adenovirus harboring human p53 cDNA (Pro89His) |
| TP53 | 3183 | Recombinant adenovirus | AxCAhp53KP1070 | Recombinant adenovirus harboring human p53 cDNA (Ser240Asn) |
| TP53 | 2807 | Recombinant adenovirus | AxCALNLhp53R273H (forward) | Recombinant adenovirus harboring human p53 cDNA (R273H) |
| TP53 | 2802 | Recombinant adenovirus | AxCALNLhp53.clone 28 (forward) | Recombinant adenovirus harboring human p53 cDNA (deletion) |
| TP53 | 2799 | Recombinant adenovirus | AxCALNLhp53P70 (forward) | Recombinant adenovirus harboring human p53 cDNA (Pro128Thr) |
| TP53 | 2798 | Recombinant adenovirus | AxCALNLhp53P68 (forward) | Recombinant adenovirus harboring human p53 cDNA |
| TP53 | 2795 | Recombinant adenovirus | AxCALNLhp53WT (forward) | Recombinant adenovirus harboring human p53 cDNA |
| TP53 | 2785 | Recombinant adenovirus | AxCAhp53KP1070 (forward) | Recombinant adenovirus harboring human p53 cDNA (Ser240Asn) |
| TP53 | 2784 | Recombinant adenovirus | AxCAhp53KP1065 (forward) | Recombinant adenovirus harboring human p53 cDNA (Ser96Pro) |
| TP53 | 2783 | Recombinant adenovirus | AxCAhp53KP1010 (forward) | Recombinant adenovirus harboring human p53 cDNA (Gly105Asp) |
| TP53 | 2782 | Recombinant adenovirus | AxCAhp53KP1007 (forward) | Recombinant adenovirus harboring human p53 cDNA (Gly374Cys) |
| TP53 | 2771 | Recombinant adenovirus | AxCAhp53.clone 28 (forward) | Recombinant adenovirus harboring human p53 cDNA (deletion) |
| TP53 | 2757 | Recombinant adenovirus | AxCAhp53P70 (forward) | Recombinant adenovirus harboring human p53 cDNA (Pro128Thr) |
| TP53 | 2756 | Recombinant adenovirus | AxCAhp53P68 (forward) | Recombinant adenovirus harboring human p53 cDNA |
| TP53 | 2755 | Recombinant adenovirus | AxCAhp53WT (forward) | Recombinant adenovirus harboring human p53 cDNA |
| TP53 | 2713 | ShuttleVector | pAxCALNLhp53R273H (reverse) | Shuttle vector to produce rAd expressing human p53 (R273H) |
| TP53 | 2712 | ShuttleVector | pAxCALNLhp53R273H (forward) | Shuttle vector to produce rAd expressing human p53 (R273H) |
| TP53 | 2630 | ShuttleVector | pAxCAhp53R273H (reverse) | Shuttle vector to produce rAd expressing human p53 (R273H) |
| TP53 | 2629 | ShuttleVector | pAxCAhp53R273H (forward) | Shuttle vector to produce rAd expressing human p53 (R273H) |
| TP53 | 2509 | ShuttleVector | pAxCAhp53P26 (reverse) | Shuttle vector to generate rAd harboring human p53 |
| TP53 | 2508 | ShuttleVector | pAxCAhp53P26 (forward) | Shuttle vector to produce rAd expressing human p53 |
| TP53 | 2507 | ShuttleVector | pAxCAhp53P25 (reverse) | Shuttle vector to generate rAd harboring human p53 |
| TP53 | 2506 | ShuttleVector | pAxCAhp53P25 (forward) | Shuttle vector to produce rAd expressing human p53 |
| TP53 | 2505 | ShuttleVector | pAxCAhp53P24 (reverse) | Shuttle vector to generate rAd harboring human p53 |
| TP53 | 2504 | ShuttleVector | pAxCAhp53P24 (forward) | Shuttle vector to produce rAd expressing human p53 |
| TP53 | 2503 | ShuttleVector | pAxCAhp53P21 (reverse) | Shuttle vector to generate rAd harboring human p53 |
| TP53 | 2502 | ShuttleVector | pAxCAhp53P21 (forward) | Shuttle vector to produce rAd expressing human p53 |
| TP53 | 2501 | ShuttleVector | pAxCAhp53P19 (reverse) | Shuttle vector to generate rAd harboring human p53 |
| TP53 | 2500 | ShuttleVector | pAxCAhp53P19 (forward) | Shuttle vector to produce rAd expressing human p53 |
| TP53 | 2499 | ShuttleVector | pAxCAhp53P17 (reverse) | Shuttle vector to generate rAd harboring human p53 |
| TP53 | 2498 | ShuttleVector | pAxCAhp53P17 (forward) | Shuttle vector to produce rAd expressing human p53 |
| TP53 | 2497 | ShuttleVector | pAxCAhp53P16 (reverse) | Shuttle vector to generate rAd harboring human p53 |
| TP53 | 2496 | ShuttleVector | pAxCAhp53P16 (forward) | Shuttle vector to produce rAd expressing human p53 |
| TP53 | 2495 | ShuttleVector | pAxCAhp53P50 (reverse) | Shuttle vector to generate rAd harboring human p53 |
| TP53 | 2494 | ShuttleVector | pAxCAhp53P50 (forward) | Shuttle vector to produce rAd expressing human p53 |
| TP53 | 2493 | ShuttleVector | pAxCAhp53P13 (reverse) | Shuttle vector to generate rAd harboring human p53 |
| TP53 | 2492 | ShuttleVector | pAxCAhp53P13 (forward) | Shuttle vector to produce rAd expressing human p53 |
| TP53 | 2437 | DNA clone | pCAGhp53R273H (Reverse) | Mammalian vector expressing mutant of p53 gene (R273H) |
| TP53 | 2436 | DNA clone | pCAGhp53P70 (Reverse) | Mammalian vector expressing mutant of p53 gene (Pro128Thr) |
| TP53 | 2435 | DNA clone | pCAGhp53P68 (Forward) | Mammalian vector expressing mutant of p53 gene (Ile 162 Val, Gly 266 Asp, frame shift) |
| TP53 | 2434 | DNA clone | pCAGhp53WT (Reverse) | Mammalian vector expressing wild type of p53 gene |
| TP53 | 2433 | DNA clone | pCAGhp53R273H (Forward) | Mammalian vector expressing mutant of p53 gene (R273H) |
| TP53 | 2432 | DNA clone | pCAGhp53clone28 (Forward) | Mammalian vector expressing the deletion mutant of p53 gene |
| TP53 | 2431 | DNA clone | pCAGhp53P70 (Forward) | Mammalian vector expressing mutant of p53 gene (Pro128Thr) |
| TP53 | 2430 | DNA clone | pCAGhp53P68 (Reverse) | Mammalian vector expressing mutant of p53 gene (Ile 162 Val, Gly 266 Asp, frame shift) |
| TP53 | 2429 | DNA clone | pCAGhp53WT (Forward) | Mammalian vector expressing wild type p53 gene |
| TP53 | 1982 | Recombinant Adenovirus | AxCALNLhp53 | Recombinant adenovirus expressing human p53 |
| MDM2 | 2198 | Recombinant Adenovirus | AxCAhMDM2 | Recombinant adenovirus expressing human MDM2 regulated by CAG promoter |
| chk1 | 2949 | DNA clone | chk1+ | Plasmid clone of fission yeast Chk1 |
| Chek1 | 6946 | H3042B08 | Chek1, NIA/NIH Mouse 15K cDNA Clone (full CDS) | |
| CDKN1A | 5893 | DNA clone | pKM2L-phP21 | Promoter Bank clone, Human p21 cyclin dependent kinase inhibitor/WAF1/CIP1 promoter |
| CDKN1A | 3576 | ShuttleVector | pAxCALNLhp21(forward) | Shuttle vector to generate rAd harboring Sugano full-length cDNA clone |
| CDKN1A | 1999 | ShuttleVector | pAxCAhp21 | Shuttle vector to generate rAd expressing human CDKI p21 |
| CDK2 | 5250 | ShuttleVector | pAxCALNLhCDK2(reverse) | Shuttle vector to generate rAd expressing human CDK2 |
| CDK2 | 5249 | ShuttleVector | pAxCALNLhCDK2(forward) | Shuttle vector to generate rAd expressing human CDK2 |
| CDK2 | 4061 | ShuttleVector | pAxCALNLhCDK2 | Shuttle vector to generate rAd harboring Sugano full-length cDNA clone |
| CDK2 | 3492 | ShuttleVector | pAxCALNLhCDK2(forward) | Shuttle vector to generate rAd harboring Sugano full-length cDNA clone |
| CDK2 | 3472 | ShuttleVector | pAxCALNLhCDK2(forward) | Shuttle vector to generate rAd harboring Sugano full-length cDNA clone |
| Cdk2 | 6947 | H3117F10 | Cdk2, NIA/NIH Mouse 15K cDNA Clone (full CDS) | |
| CDC25A | 5476 | DNA clone | pKM2L-phCDC25A | Promoter Bank clone, Human cdc25A promoter |
| CDC25A | 4653 | ShuttleVector | pAxCALNLhCDC25A(forward) | Shuttle vector to generate rAd expressing human CDC25A |
| CDC25A | 4646 | ShuttleVector | pAxCALNLhCDC25A(reverse) | Shuttle vector to generate rAd expressing human CDC25A |
(GSB0009e,T.M. 2004.04.14)
2018.09.24